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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFB876
Library VF
(Link to library)
Clone ID VFB876
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16272-1
Original site URL
Representative seq. ID VFB876P
(Link to Original site)
Representative DNA sequence 
>VFB876 (VFB876Q) /CSM/VF/VFB8-D/VFB876Q.Seq.d/
CGTTAGTCACTTCTAAACAACAATAACAATATGAGTCATCGTAAATTTGAAGCTCCACGT
CACGGTAATCTCGGTTTCAGACCAAGAAAGAGAGCCGCCAGACATCAAGGTAAAGTCAAG
TCATTCCCAAAGGATGACCGTACCCAAAAAGTCCACTTAACTGCATTCATGGGTTACAAA
GCTGGTATGACTCACGTTGTCAGAGATTTAGAAAAACCAGGTTCAAAGATGCACAAAAAA
GAAATCGTTGAAGCCGTCACCATCATTGAATGTCCACCAATGTACATTGTTGGTTTAGTT
GGTTATGTTGAAACCGCTCAAGGTCTCAAAACCTACAAAACCGTCTGGGCTCAACATTTA
AGTGATAACTTCCGTAGAAGATTATACAAAAACTGGTACAAATCAAAXXXXXXXXXXAGT
CAATGGTGGTTCAATCGTCGAAAAAGTTAACTTTGCTGTTGCCAACTTTGAAAAGACCGT
CAACGTCACTGGTGTCTTTGCCGAAAATGAATTAATCGATGTTATCGGTGTAACCAAAGG
TAAAGGTTTCAATGGTGTCATTAAAAGATGGGGTGTCCGTAAATTACCAAGAAAGACCCA
TAAAGGTTTAAGAAAGGTTGCCTGTATTGGTGCATGGCATCCATCCCGTGTTAGTACCAC
CGTCCCAAGAGCTGGTCAACTCGGTTACCATCATCGTGTCGAAAGAAACAAGAAGATTTA
CAGAATCGGTCAAGCTCAACCAGAAGACGGTAAACAAATCTCAACCGGTAAGACTGAATT
CGATTTAACTGAAAAGACTATCAATCCAATGGGTGGTTTCGCTCACTACGGTATGGTTAA
ACATGAATTCTTAATGCTTAAAGGTTGTGTTGCTGGTCCAAGAAAGAGAGCCTTAACCTT
AAGAAAGAGTATCACCACTCAAACTGGTCGTGCTGCCCTCGAGAAAATCACTCTCAAATT
CATCGATACCTCCAGCAAGTTTGGTCACGGTCTTCATCAAACCGCTGAAGATAAGACTAA
ATATTTCGGCGTCAAGAAATCAAGATCAACTAAGGCTTAAATTCAATAAATTAGATTTCA
GAGAGTTATCAAACA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
r*SLLNNNNNMSHRKFEAPRHGNLGFRPRKRAARHQGKVKSFPKDDRTQKVHLTAFMGYK
AGMTHVVRDLEKPGSKMHKKEIVEAVTIIECPPMYIVGLVGYVETAQGLKTYKTVWAQHL
SDNFRRRLYKNWYKS---

---VNGGSIVEKVNFAVANFEKTVNVTGVFAENELIDVIGVTKGKGFNGVIKRWGVRKLP
RKTHKGLRKVACIGAWHPSRVSTTVPRAGQLGYHHRVERNKKIYRIGQAQPEDGKQISTG
KTEFDLTEKTINPMGGFAHYGMVKHEFLMLKGCVAGPRKRALTLRKSITTQTGRAALEKI
TLKFIDTSSKFGHGLHQTAEDKTKYFGVKKSRSTKA*iq*irfqrvik
Translated Amino Acid sequence (All Frames) 
Frame A:
r*SLLNNNNNMSHRKFEAPRHGNLGFRPRKRAARHQGKVKSFPKDDRTQKVHLTAFMGYK
AGMTHVVRDLEKPGSKMHKKEIVEAVTIIECPPMYIVGLVGYVETAQGLKTYKTVWAQHL
SDNFRRRLYKNWYKS---

---sqwwfnrrks*lcccql*kdrqrhwclcrk*inrcyrcnqr*rfqwch*kmgcp*it
kkdp*rfkkgclywcmasipc*yhrpkswstrlpsscrkkqedlqnrssstrrr*tnlnr
*d*irfn*kdyqsngwfrslryg*t*ilna*rlccwskkeslnlkkeyhhsnwsccpren
hsqihrylqqvwsrsssnr*r*d*ifrrqeikin*glnsin*isesyqt

Frame B:
vshf*ttiti*vivnlklhvtvisvsdqereppdikvksshsqrmtvpkkst*lhswvtk
lv*ltlsei*knqvqrctkkkslkpspslnvhqctllv*lvmlkplkvskptkpsglni*
vitsvedytktgtnq---

---VNGGSIVEKVNFAVANFEKTVNVTGVFAENELIDVIGVTKGKGFNGVIKRWGVRKLP
RKTHKGLRKVACIGAWHPSRVSTTVPRAGQLGYHHRVERNKKIYRIGQAQPEDGKQISTG
KTEFDLTEKTINPMGGFAHYGMVKHEFLMLKGCVAGPRKRALTLRKSITTQTGRAALEKI
TLKFIDTSSKFGHGLHQTAEDKTKYFGVKKSRSTKA*iq*irfqrvik

Frame C:
lvtskqq*qyess*i*sstsr*srfqtkkesrqtsr*sqvipkg*pypksplncihglqs
wydsrcqrfrktrfkdaqkrnr*srhhh*mstnvhcwfswlc*nrsrsqnlqnrlgstfk
**lp*kiiqklvqik---

---smvvqsskkltlllptlkrpstslvslpkmn*smlsv*pkvkvsmvslkdgvsvnyq
erpikv*erlpvlvhgihpvlvppsqelvnsvtiivsketrrftesvklnqktvnksqpv
rlnsi*lkrlsiqwvvslttvwlnmns*clkvvllvqerep*p*ervsplklvvlpsrks
lsnssippaslvtvfikplkirlnisasrnqdqlrlkfnkldfrelsn
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFB876 (VFB876Q) /CSM/VF/VFB8-D/VFB876Q.Seq.d/                       2111   0.0
VFL717 (VFL717Q) /CSM/VF/VFL7-A/VFL717Q.Seq.d/                       1344   0.0
VFL402 (VFL402Q) /CSM/VF/VFL4-A/VFL402Q.Seq.d/                       1344   0.0
VFI836 (VFI836Q) /CSM/VF/VFI8-B/VFI836Q.Seq.d/                       1344   0.0
VFI645 (VFI645Q) /CSM/VF/VFI6-B/VFI645Q.Seq.d/                       1344   0.0
VFI571 (VFI571Q) /CSM/VF/VFI5-C/VFI571Q.Seq.d/                       1344   0.0
VFI112 (VFI112Q) /CSM/VF/VFI1-A/VFI112Q.Seq.d/                       1344   0.0
VFH886 (VFH886Q) /CSM/VF/VFH8-D/VFH886Q.Seq.d/                       1344   0.0
VFF813 (VFF813Q) /CSM/VF/VFF8-A/VFF813Q.Seq.d/                       1344   0.0
VFF444 (VFF444Q) /CSM/VF/VFF4-B/VFF444Q.Seq.d/                       1344   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

L08391|L08391.1 Dictyostelium discoideum ribosomal protein (L3) gene, complete cds.   888  0.0  4
AX489150|AX489150.1 Sequence 6450 from Patent WO02053728.         78  7e-39  8
Z74971|Z74971.1 S.cerevisiae chromosome XV reading frame ORF YOR063w.    70  1e-31  7
J01351|J01351.1 S.cerevisiae ribosomal protein L3 (tcm1) gene.    62  1e-30  7
Z70678|Z70678.1 S.cerevisiae chromosome XV DNA, 54.7 kb region.    70  1e-24  8
AX765804|AX765804.1 Sequence 13 from Patent WO03008440.           74  2e-19  4
AY052743|AY052743.1 Arabidopsis thaliana At1g43170/F1I21_18 mRNA, complete cds.    74  2e-19  4
AF361101|AF361101.1 Arabidopsis thaliana putative L3 cytoplasmic ribosomal protein (F1I21.L3) mRNA, complete cds.    74  2e-19  4
AY057487|AY057487.1 Arabidopsis thaliana At1g43170/F1I21_18 mRNA, complete cds.    74  3e-19  4
AF361809|AF361809.1 Arabidopsis thaliana At1g43170/F1I21_18 gene, complete cds.    74  3e-19  4
dna update 2003. 9.10
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AK092695_1(AK092695|pid:none) Homo sapiens cDNA FLJ35376 fis, cl...   261   e-122
(P34113) RecName: Full=60S ribosomal protein L3;  &JC4254(JC4254...   432   e-119
Z49936_3(Z49936|pid:none) Caenorhabditis elegans Cosmid F13B10, ...   232   e-109
DQ241849_1(DQ241849|pid:none) Solanum tuberosum clone 187E04 rib...   278   2e-73
BC158279_1(BC158279|pid:none) Xenopus tropicalis hypothetical pr...   277   4e-73
EF070522_1(EF070522|pid:none) Maconellicoccus hirsutus clone WHM...   276   9e-73
BT070643_1(BT070643|pid:none) Picea sitchensis clone WS02742_A15...   276   1e-72
BT034517_1(BT034517|pid:none) Zea mays full-length cDNA clone ZM...   275   2e-72
BT036034_1(BT036034|pid:none) Zea mays full-length cDNA clone ZM...   275   2e-72
AY395739_1(AY395739|pid:none) Nicotiana tabacum ribosomal protei...   275   2e-72
protein update 2009. 6.17
PSORT 

 psg: 0.60  gvh: 0.20  alm: 0.36  top: 0.53  tms: 0.00  mit: 0.37  mip: 0.06
 nuc: 0.27  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.50  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 80.0 %: cytoplasmic
	 16.0 %: mitochondrial
	  4.0 %: nuclear

>> prediction for VFB876 is cyt
5' end seq. ID VFB876F
5' end seq. 
>VFB876F.Seq
CGTTAGTCACTTCTAAACAACAATAACAATATGAGTCATCGTAAATTTGAAGCTCCACGT
CACGGTAATCTCGGTTTCAGACCAAGAAAGAGAGCCGCCAGACATCAAGGTAAAGTCAAG
TCATTCCCAAAGGATGACCGTACCCAAAAAGTCCACTTAACTGCATTCATGGGTTACAAA
GCTGGTATGACTCACGTTGTCAGAGATTTAGAAAAACCAGGTTCAAAGATGCACAAAAAA
GAAATCGTTGAAGCCGTCACCATCATTGAATGTCCACCAATGTACATTGTTGGTTTAGTT
GGTTATGTTGAAACCGCTCAAGGTCTCAAAACCTACAAAACCGTCTGGGCTCAACATTTA
AGTGATAACTTCCGTAGAAGATTATACAAAAACTGGTACAAATCAAA----------
Length of 5' end seq. 407
3' end seq. ID VFB876Z
3' end seq. 
>VFB876Z.Seq
----------AGTCAATGGTGGTTCAATCGTCGAAAAAGTTAACTTTGCTGTTGCCAACT
TTGAAAAGACCGTCAACGTCACTGGTGTCTTTGCCGAAAATGAATTAATCGATGTTATCG
GTGTAACCAAAGGTAAAGGTTTCAATGGTGTCATTAAAAGATGGGGTGTCCGTAAATTAC
CAAGAAAGACCCATAAAGGTTTAAGAAAGGTTGCCTGTATTGGTGCATGGCATCCATCCC
GTGTTAGTACCACCGTCCCAAGAGCTGGTCAACTCGGTTACCATCATCGTGTCGAAAGAA
ACAAGAAGATTTACAGAATCGGTCAAGCTCAACCAGAAGACGGTAAACAAATCTCAACCG
GTAAGACTGAATTCGATTTAACTGAAAAGACTATCAATCCAATGGGTGGTTTCGCTCACT
ACGGTATGGTTAAACATGAATTCTTAATGCTTAAAGGTTGTGTTGCTGGTCCAAGAAAGA
GAGCCTTAACCTTAAGAAAGAGTATCACCACTCAAACTGGTCGTGCTGCCCTCGAGAAAA
TCACTCTCAAATTCATCGATACCTCCAGCAAGTTTGGTCACGGTCTTCATCAAACCGCTG
AAGATAAGACTAAATATTTCGGCGTCAAGAAATCAAGATCAACTAAGGCTTAAATTCAAT
AAATTAGATTTCAGAGAGTTATCAAACA
Length of 3' end seq. 678
Connected seq. ID VFB876P
Connected seq. 
>VFB876P.Seq
CGTTAGTCACTTCTAAACAACAATAACAATATGAGTCATCGTAAATTTGAAGCTCCACGT
CACGGTAATCTCGGTTTCAGACCAAGAAAGAGAGCCGCCAGACATCAAGGTAAAGTCAAG
TCATTCCCAAAGGATGACCGTACCCAAAAAGTCCACTTAACTGCATTCATGGGTTACAAA
GCTGGTATGACTCACGTTGTCAGAGATTTAGAAAAACCAGGTTCAAAGATGCACAAAAAA
GAAATCGTTGAAGCCGTCACCATCATTGAATGTCCACCAATGTACATTGTTGGTTTAGTT
GGTTATGTTGAAACCGCTCAAGGTCTCAAAACCTACAAAACCGTCTGGGCTCAACATTTA
AGTGATAACTTCCGTAGAAGATTATACAAAAACTGGTACAAATCAAA----------AGT
CAATGGTGGTTCAATCGTCGAAAAAGTTAACTTTGCTGTTGCCAACTTTGAAAAGACCGT
CAACGTCACTGGTGTCTTTGCCGAAAATGAATTAATCGATGTTATCGGTGTAACCAAAGG
TAAAGGTTTCAATGGTGTCATTAAAAGATGGGGTGTCCGTAAATTACCAAGAAAGACCCA
TAAAGGTTTAAGAAAGGTTGCCTGTATTGGTGCATGGCATCCATCCCGTGTTAGTACCAC
CGTCCCAAGAGCTGGTCAACTCGGTTACCATCATCGTGTCGAAAGAAACAAGAAGATTTA
CAGAATCGGTCAAGCTCAACCAGAAGACGGTAAACAAATCTCAACCGGTAAGACTGAATT
CGATTTAACTGAAAAGACTATCAATCCAATGGGTGGTTTCGCTCACTACGGTATGGTTAA
ACATGAATTCTTAATGCTTAAAGGTTGTGTTGCTGGTCCAAGAAAGAGAGCCTTAACCTT
AAGAAAGAGTATCACCACTCAAACTGGTCGTGCTGCCCTCGAGAAAATCACTCTCAAATT
CATCGATACCTCCAGCAAGTTTGGTCACGGTCTTCATCAAACCGCTGAAGATAAGACTAA
ATATTTCGGCGTCAAGAAATCAAGATCAACTAAGGCTTAAATTCAATAAATTAGATTTCA
GAGAGTTATCAAACA
Length of connected seq. 1085
Full length Seq ID -
Full length Seq. -
Length of full length seq. -