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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFB874
Library VF
(Link to library)
Clone ID VFB874
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16176-1
Original site URL
Representative seq. ID VFB874P
(Link to Original site)
Representative DNA sequence 
>VFB874 (VFB874Q) /CSM/VF/VFB8-D/VFB874Q.Seq.d/
AAAAAAAAAATTTTCAAATCACAATTTTAACTTATCATCATTCAAAAATGTTGTCTTCAA
TTTTAAAAAAAATTCAACCATCATTATTAGTTAACTCTAGAATTATCACTAGAACTTATG
CTACTAAAGAGGTTACAGTTCGTGATGCAATTAATAGTGCATTGGATGAAGAATTAGCAA
GAGATGAAAAAGTTTTTATTATGGGTGAAGAAGTAGCTCAATATAATGGTGCATACAAGA
TCACTAAAGGATTATTCGATAAATATGGTGGTGATAGAATTATTGATACTCCAATTACAG
AAGCTGGTTTTGCTGGTATTGGTGTTGGTGCTGCTATGGCAGGTNCTAGACCAATCATTG
AATTTATGACTTTCAACTTTGCTATGCAAGCAATTGATCACATCATCAATTCATCTGCTA
AAACTCATTATNTGTCTGGTGGTAAAGTTTTCAATCCAATCGTTTGGAGAGGTCCAAATG
GTCCACCAACTGCTGTTGGTXXXXXXXXXXGNNGNNAAAAAAGNTNCCCNAAAAAANNAA
NNAATTTTAAAAAAAAAAAGTCCTTAATTAATTTTTTCCCCCNAAAAAAAAGAAANAANN
ATAAAGAAAAAATAAAAAAAAAAAAAAATT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
KKNFQITILTYHHSKMLSSILKKIQPSLLVNSRIITRTYATKEVTVRDAINSALDEELAR
DEKVFIMGEEVAQYNGAYKITKGLFDKYGGDRIIDTPITEAGFAGIGVGAAMAGXRPIIE
FMTFNFAMQAIDHIINSSAKTHYXSGGKVFNPIVWRGPNGPPTAVG---

---XXKXXPKKXXNFKKKKSLINFFPXKKRXXXKEKIKKKKN
Translated Amino Acid sequence (All Frames) 
Frame A:
kkkifksqf*liiiqkcclqf*kkfnhhy*ltlelslelmllkrlqfvmqlivhwmkn*q
emkkfllwvkk*lnimvhtrslkdysinmvviellilqlqklvllvlvlvllwqvldqsl
nl*lstllckqlitssihllklixclvvkfsiqsfgevqmvhqlll---

---xxkkxxxkxxxilkkksp*lifspxkkexxikkk*kkkki

Frame B:
kkkfsnhnfnlssfknvvfnfkknstiiis*l*nyh*nlcy*rgyss*cn**cig*risk
r*ksfyyg*rsssi*wciqdh*riir*iww**ny*ysnyrswfcwywcwccygrx*tnh*
iydfqlcyasn*shhqfic*nslxvww*sfqsnrlerskwstnccw---

---xxkkxpxkxxxf*kkkvln*ffppkkkkxx*rknkkkkk

Frame C:
KKNFQITILTYHHSKMLSSILKKIQPSLLVNSRIITRTYATKEVTVRDAINSALDEELAR
DEKVFIMGEEVAQYNGAYKITKGLFDKYGGDRIIDTPITEAGFAGIGVGAAMAGXRPIIE
FMTFNFAMQAIDHIINSSAKTHYXSGGKVFNPIVWRGPNGPPTAVG---

---XXKXXPKKXXNFKKKKSLINFFPXKKRXXXKEKIKKKKN
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFO292 (VFO292Q) /CSM/VF/VFO2-D/VFO292Q.Seq.d/                        912   0.0
VFB874 (VFB874Q) /CSM/VF/VFB8-D/VFB874Q.Seq.d/                        912   0.0
FC-BN12 (FC-BN12Q) /CSM/FC/FC-BN/FC-BN12Q.Seq.d/                      912   0.0
CHB533 (CHB533Q) /CSM/CH/CHB5-B/CHB533Q.Seq.d/                        912   0.0
FC-537 (FC-537Q) /CSM/CG/CG-FC/FC-537Q.Seq.d/                         912   0.0
VFI440 (VFI440Q) /CSM/VF/VFI4-B/VFI440Q.Seq.d/                        900   0.0
VFK807 (VFK807Q) /CSM/VF/VFK8-A/VFK807Q.Seq.d/                        896   0.0
SFC894 (SFC894Q) /CSM/SF/SFC8-D/SFC894Q.Seq.d/                        896   0.0
SFD157 (SFD157Q) /CSM/SF/SFD1-C/SFD157Q.Seq.d/                        894   0.0
VFO521 (VFO521Q) /CSM/VF/VFO5-A/VFO521Q.Seq.d/                        892   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC114263|AC114263.2 Dictyostelium discoideum chromosome 2 map 215673-367476 strain AX4, complete sequence.   724  0.0  3
BQ855000|BQ855000.1 QGB24M14.yg.ab1 QG_ABCDI lettuce salinas Lactuca sativa cDNA clone QGB24M14, mRNA sequence.    60  2e-19  5
BQ862971|BQ862971.1 QGC22J22.yg.ab1 QG_ABCDI lettuce salinas Lactuca sativa cDNA clone QGC22J22, mRNA sequence.    60  3e-19  5
BQ860256|BQ860256.1 QGC15E06.yg.ab1 QG_ABCDI lettuce salinas Lactuca sativa cDNA clone QGC15E06, mRNA sequence.    60  7e-19  5
BU881003|BU881003.1 UM57TE08 Populus flower cDNA library Populus balsamifera subsp. trichocarpa cDNA 5 prime, mRNA sequence.    64  3e-18  3
BI073208|BI073208.1 PIP2B_G6 Sugar Beet germination cDNA library Beta vulgaris cDNA 5' similar to pyruvate dehydrogenase, mRNA sequence.    64  4e-18  4
BY227294|BY227294.1 Mus musculus erythroblast cDNA, RIKEN full-length enriched library, clone:K0C0009D03, 5' end partial sequence.    52  1e-17  4
BI121766|BI121766.1 F046P35Y Populus flower cDNA library Populus balsamifera subsp. trichocarpa cDNA, mRNA sequence.    64  3e-17  2
BI120224|BI120224.1 F012P16Y Populus flower cDNA library Populus balsamifera subsp. trichocarpa cDNA, mRNA sequence.    64  3e-17  2
BQ862298|BQ862298.1 QGC20L13.yg.ab1 QG_ABCDI lettuce salinas Lactuca sativa cDNA clone QGC20L13, mRNA sequence.    60  4e-17  4
dna update 2003. 9.10
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q86HX0) RecName: Full=Pyruvate dehydrogenase E1 component subun...   262   5e-69
EU962529_1(EU962529|pid:none) Zea mays clone 243474 pyruvate deh...   170   3e-41
AF069910_1(AF069910|pid:none) Zea mays pyruvate dehydrogenase E1...   170   3e-41
AK104686_1(AK104686|pid:none) Oryza sativa Japonica Group cDNA c...   164   2e-39
AM494962_228(AM494962|pid:none) Leishmania braziliensis chromoso...   164   2e-39
AK104686_2(AK104686|pid:none) Oryza sativa Japonica Group cDNA c...   163   3e-39
AM502243_181(AM502243|pid:none) Leishmania infantum chromosome 25.    162   6e-39
(Q38799) RecName: Full=Pyruvate dehydrogenase E1 component subun...   162   6e-39
U09137_1(U09137|pid:none) Arabidopsis thaliana pyruvate dehydrog...   162   6e-39
EF144284_1(EF144284|pid:none) Populus trichocarpa clone PX0015_F...   162   6e-39
protein update 2009. 6.17
PSORT 

 psg: 0.86  gvh: 0.44  alm: 0.41  top: 0.60  tms: 0.00  mit: 0.50  mip: 0.06
 nuc: 0.20  erl: 0.00  erm: 0.80  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: mitochondrial
	 40.0 %: nuclear
	 12.0 %: cytoplasmic
	  4.0 %: endoplasmic reticulum
	  4.0 %: peroxisomal

>> prediction for VFB874 is mit
5' end seq. ID VFB874F
5' end seq. 
>VFB874F.Seq
AAAAAAAAAATTTTCAAATCACAATTTTAACTTATCATCATTCAAAAATGTTGTCTTCAA
TTTTAAAAAAAATTCAACCATCATTATTAGTTAACTCTAGAATTATCACTAGAACTTATG
CTACTAAAGAGGTTACAGTTCGTGATGCAATTAATAGTGCATTGGATGAAGAATTAGCAA
GAGATGAAAAAGTTTTTATTATGGGTGAAGAAGTAGCTCAATATAATGGTGCATACAAGA
TCACTAAAGGATTATTCGATAAATATGGTGGTGATAGAATTATTGATACTCCAATTACAG
AAGCTGGTTTTGCTGGTATTGGTGTTGGTGCTGCTATGGCAGGTNCTAGACCAATCATTG
AATTTATGACTTTCAACTTTGCTATGCAAGCAATTGATCACATCATCAATTCATCTGCTA
AAACTCATTATNTGTCTGGTGGTAAAGTTTTCAATCCAATCGTTTGGAGAGGTCCAAATG
GTCCACCAACTGCTGTTGGT----------
Length of 5' end seq. 500
3' end seq. ID VFB874Z
3' end seq. 
>VFB874Z.Seq
----------GNNGNNAAAAAAGNTNCCCNAAAAAANNAANNAATTTTAAAAAAAAAAAG
TCCTTAATTAATTTTTTCCCCCNAAAAAAAAGAAANAANNATAAAGAAAAAATAAAAAAA
AAAAAAAATT
Length of 3' end seq. 120
Connected seq. ID VFB874P
Connected seq. 
>VFB874P.Seq
AAAAAAAAAATTTTCAAATCACAATTTTAACTTATCATCATTCAAAAATGTTGTCTTCAA
TTTTAAAAAAAATTCAACCATCATTATTAGTTAACTCTAGAATTATCACTAGAACTTATG
CTACTAAAGAGGTTACAGTTCGTGATGCAATTAATAGTGCATTGGATGAAGAATTAGCAA
GAGATGAAAAAGTTTTTATTATGGGTGAAGAAGTAGCTCAATATAATGGTGCATACAAGA
TCACTAAAGGATTATTCGATAAATATGGTGGTGATAGAATTATTGATACTCCAATTACAG
AAGCTGGTTTTGCTGGTATTGGTGTTGGTGCTGCTATGGCAGGTNCTAGACCAATCATTG
AATTTATGACTTTCAACTTTGCTATGCAAGCAATTGATCACATCATCAATTCATCTGCTA
AAACTCATTATNTGTCTGGTGGTAAAGTTTTCAATCCAATCGTTTGGAGAGGTCCAAATG
GTCCACCAACTGCTGTTGGT----------GNNGNNAAAAAAGNTNCCCNAAAAAANNAA
NNAATTTTAAAAAAAAAAAGTCCTTAATTAATTTTTTCCCCCNAAAAAAAAGAAANAANN
ATAAAGAAAAAATAAAAAAAAAAAAAAATT
Length of connected seq. 620
Full length Seq ID -
Full length Seq. -
Length of full length seq. -