>VFB864 (VFB864Q) /CSM/VF/VFB8-C/VFB864Q.Seq.d/
GTAGTATTTTCTGTACATATATTAATAGAATTGTAATAAAATGTCAAAAGTAATTACAGG
AAAGCAATACTCTTGGTCAGAGTTAGCCAAACATAACACTGAAAATGATTGTTGGGTTGC
AGTCGATGGTAAAGTTTATGATATTACAAGATGGGTACCATTACATCCAGGTGGTAAAGA
AGTTTTACTTTTAGCAGCAGGTAGAGATGTTACAAATTTATTTGAAAGTTATCATCCAAT
GTCAGATAAACCAACATCAATTTTAAAGAATTATGAAATTGGATATATTTCATCATATGA
ACATCCAAAATTTGTTCAAAAATCAGATTTCTACAAGACATTAAAAGAGAGAGTTAGAAA
ACATTTTAAAGCAACCGATCAAGATCCACAAATGGCAGTTAGTATTTTCTCACGTTTAGC
ATTGGTTTATCTTTTAGTGTTTGTAACTTACTATTTAGCACATTATACCAGTAACAATTT
TTATTTAAATTGTTTCTTGGCAATCGTTTACGCACTTTGCAACTCATTATTCTCAATGCA
TATGATGCATGATTCATGTCATGCTGCCATTTCACATTATCCAGGCGTATGGAAATGGAT
GGGCGCATCATTTGATTTCGTCACTGGTGCTTCATTCCTTTCATGGTGTCATCAACATGT
AATTGGTCATCAXXXXXXXXXXTTGTCACTCCATTCCAAACTCGTTCATGGTATCATAAA
TATCAACACATTTACGCTCCACTCCTCTATGGTATTTACACACTCAAATATCGTACTCAA
GATTGGGAAGCTTTTGTAAAGGATGGTAAAAATGGTGCAATTCGTGTTAGTGTCGCCACA
AATTTCGATAAGGCCGCTTACGTCATTGGTAAATTGTCTTTTGTTTTCTTCCGTTTCATC
CTTCCACTCCGTTATCATAGCTTTACAGATTTAATTTGTTATTTCCTCATTGCTGAATTC
GTCTTTGGTTGGTATCTCACAATTAATTTCCAAGTTAGTCATGTCGCTGAAGATCTCAAA
TTCTTTGCTACCCCTGAAAGACCAGATGAACCATCTCAAATCAATGAAGATTGGGCAATC
CTTCAACTTAAAACTACTCAAGATTATGGTCATGGTTCACTCCTTTGTACCTTTTTTAGT
GGTTCTTTAAATCATCAAGTTGTTCATCATTTATTCCCATCAATTGCTCAAGATTTCTAC
CCACAACTTGTACCAATTGTAAAAGAAGTTTGTAAAGAACATAACATTACTTACCACATT
AAACCAAACTTCACTGAAGCTATTATGTCACACATTAATTACCTTTACAAAATGGGTAAT
GATCCAGATTATGTTAAAAAACCATAGCCTCAAAAGA

*yflyiy**NCNKMSKVITGKQYSWSELAKHNTENDCWVAVDGKVYDITRWVPLHPGGKE
VLLLAAGRDVTNLFESYHPMSDKPTSILKNYEIGYISSYEHPKFVQKSDFYKTLKERVRK
HFKATDQDPQMAVSIFSRLALVYLLVFVTYYLAHYTSNNFYLNCFLAIVYALCNSLFSMH
MMHDSCHAAISHYPGVWKWMGASFDFVTGASFLSWCHQHVIGH---

---VTPFQTRSWYHKYQHIYAPLLYGIYTLKYRTQDWEAFVKDGKNGAIRVSVATNFDKA
AYVIGKLSFVFFRFILPLRYHSFTDLICYFLIAEFVFGWYLTINFQVSHVAEDLKFFATP
ERPDEPSQINEDWAILQLKTTQDYGHGSLLCTFFSGSLNHQVVHHLFPSIAQDFYPQLVP
IVKEVCKEHNITYHIKPNFTEAIMSHINYLYKMGNDPDYVKKP*pqk

Frame A:
vvfsvhiliel**nvksnyrkaillvrvsqt*h*k*llgcsrw*sl*yykmgtitsrw*r
sftfssr*rcykfi*klssnvr*tninfkel*nwiyfii*tskicskirflqdikres*k
tf*snrsrstngs*yfltfsiglsfsvcnllfstlyq*qflfklflgnrlrtlqliilna
yda*fmscchftlsrrmemdgrii*frhwcfipfmvsstcnwss---

---lslhsklvhgiinintftlhssmvfthsnivlkigkll*rmvkmvqfvlvspqisir
pltslvncllfssvssfhsviialqi*fvissllnsslvgisqlisklvmslkisnsllp
lkdqmnhlksmkigqsfnlkllkimvmvhsfvpflvvl*iiklfiiyshqllkisthnly
ql*kkfvknitllttlnqtslkllchtlitftkwvmiqimlknhslkr

Frame B:
*yflyiy**NCNKMSKVITGKQYSWSELAKHNTENDCWVAVDGKVYDITRWVPLHPGGKE
VLLLAAGRDVTNLFESYHPMSDKPTSILKNYEIGYISSYEHPKFVQKSDFYKTLKERVRK
HFKATDQDPQMAVSIFSRLALVYLLVFVTYYLAHYTSNNFYLNCFLAIVYALCNSLFSMH
MMHDSCHAAISHYPGVWKWMGASFDFVTGASFLSWCHQHVIGH---

---chsipnsfmvs*isthlrstplwylhtqisysrlgsfckgw*kwcnsc*crhkfr*g
rlrhw*ivfcflpfhpstpls*lyrfnllfphc*irlwlvshn*fps*scr*rsqilcyp
*ktr*tisnq*rlgnpst*nysrlwswftplylf*wffkssscssfipincsrflpttct
nckrsl*rt*hylph*tklh*syyvth*lplqng**srlc*ktiask

Frame C:
sifctyinrivikcqk*lqesntlgqs*pnitlkmivglqsmvkfmilqdgyhyiqvvkk
fyf*qqvemlqiylkviiqcqinqhqf*rimkldifhhmniqnlfknqistrh*krelen
ilkqpikihkwqlvfshv*hwfif*cl*lti*hiipvtifi*ivswqsfthfathysqci
*cmihvmlpfhiiqaygngwahhlisslvlhsfhgvinm*lvi---

---VTPFQTRSWYHKYQHIYAPLLYGIYTLKYRTQDWEAFVKDGKNGAIRVSVATNFDKA
AYVIGKLSFVFFRFILPLRYHSFTDLICYFLIAEFVFGWYLTINFQVSHVAEDLKFFATP
ERPDEPSQINEDWAILQLKTTQDYGHGSLLCTFFSGSLNHQVVHHLFPSIAQDFYPQLVP
IVKEVCKEHNITYHIKPNFTEAIMSHINYLYKMGNDPDYVKKP*pqk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFB864 (VFB864Q) /CSM/VF/VFB8-C/VFB864Q.Seq.d/                       2631   0.0
VFL212 (VFL212Q) /CSM/VF/VFL2-A/VFL212Q.Seq.d/                       1332   0.0
VFD240 (VFD240Q) /CSM/VF/VFD2-B/VFD240Q.Seq.d/                       1332   0.0
VFA219 (VFA219Q) /CSM/VF/VFA2-A/VFA219Q.Seq.d/                       1332   0.0
VFF662 (VFF662Q) /CSM/VF/VFF6-C/VFF662Q.Seq.d/                       1330   0.0
VFF269 (VFF269Q) /CSM/VF/VFF2-C/VFF269Q.Seq.d/                       1328   0.0
VFK616 (VFK616Q) /CSM/VF/VFK6-A/VFK616Q.Seq.d/                       1324   0.0
VFI746 (VFI746Q) /CSM/VF/VFI7-B/VFI746Q.Seq.d/                       1324   0.0
VFE188 (VFE188Q) /CSM/VF/VFE1-D/VFE188Q.Seq.d/                       1324   0.0
VFA320 (VFA320Q) /CSM/VF/VFA3-A/VFA320Q.Seq.d/                       1324   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

BD092935|BD092935.1 Methods and compositions for synthesis of long chain polyunsaturated fatty acids in plants.  1312  0.0  2
BD082645|BD082645.1 Methods and compositions for synthesis of long chain polyunsaturated fatty acids.  1312  0.0  2
BD082630|BD082630.1 Methods and compositions for synthesis of long chain poly-unsaturated fatty acids.  1312  0.0  2
AB029311|AB029311.1 Dictyostelium discoideum Dd des5 gene for fatty acid desaturase, complete cds.  1041  0.0  5
AB022097|AB022097.1 Dictyostelium discoideum mRNA for delta 5 fatty acid desaturase, complete cds.   149  e-126  12
AL355612|AL355612.8 Human DNA sequence from clone RP11-343P23 on chromosome 6 Contains  GSSs.    42  0.19  3
AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    34  0.25  16
AC137050|AC137050.1 Rattus norvegicus clone CH230-unknown, *** SEQUENCING IN PROGRESS ***, 2 unordered pieces.    48  0.30  1
AC117308|AC117308.7 Rattus norvegicus clone CH230-216M16, *** SEQUENCING IN PROGRESS ***, 2 unordered pieces.    48  0.30  1
AC117128|AC117128.8 Rattus norvegicus clone CH230-230O8, *** SEQUENCING IN PROGRESS ***, 5 unordered pieces.    48  0.30  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q9Y1W0) RecName: Full=Delta(5) fatty acid desaturase A;        ...   467   0.0
(O96099) RecName: Full=Delta(5) fatty acid desaturase B;        ...   323   e-165
(Q1ZXQ5) RecName: Full=Probable Delta(5) fatty acid desaturase C...   273   e-145
AF419297_1(AF419297|pid:none) Pythium irregulare delta-5 fatty a...   170   5e-83
AB188307_1(AB188307|pid:none) Mortierella alpina gene for delta5...   162   9e-78
(O74212) RecName: Full=Delta(5) fatty acid desaturase;          ...   157   6e-77
CQ831428_1(CQ831428|pid:none) Sequence 9 from Patent WO2004057001.    142   9e-63
EF678404_1(EF678404|pid:none) Picea sitchensis clone WS0291_A04 ...   145   9e-63
CP001574_328(CP001574|pid:none) Micromonas sp. RCC299 chromosome...   146   2e-60
AM949596_1(AM949596|pid:none) Mantoniella squamata d5 gene for A...   132   4e-60

 psg: 0.70  gvh: 0.24  alm: 0.25  top: 0.63  tms: 0.07  mit: 0.31  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.80  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.05  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 1.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: cytoplasmic
	 24.0 %: mitochondrial
	 24.0 %: nuclear
	  8.0 %: plasma membrane
	  8.0 %: endoplasmic reticulum
	  4.0 %: vesicles of secretory system
	  4.0 %: peroxisomal

>> prediction for VFB864 is cyt

>VFB864F.Seq
GTAGTATTTTCTGTACATATATTAATAGAATTGTAATAAAATGTCAAAAGTAATTACAGG
AAAGCAATACTCTTGGTCAGAGTTAGCCAAACATAACACTGAAAATGATTGTTGGGTTGC
AGTCGATGGTAAAGTTTATGATATTACAAGATGGGTACCATTACATCCAGGTGGTAAAGA
AGTTTTACTTTTAGCAGCAGGTAGAGATGTTACAAATTTATTTGAAAGTTATCATCCAAT
GTCAGATAAACCAACATCAATTTTAAAGAATTATGAAATTGGATATATTTCATCATATGA
ACATCCAAAATTTGTTCAAAAATCAGATTTCTACAAGACATTAAAAGAGAGAGTTAGAAA
ACATTTTAAAGCAACCGATCAAGATCCACAAATGGCAGTTAGTATTTTCTCACGTTTAGC
ATTGGTTTATCTTTTAGTGTTTGTAACTTACTATTTAGCACATTATACCAGTAACAATTT
TTATTTAAATTGTTTCTTGGCAATCGTTTACGCACTTTGCAACTCATTATTCTCAATGCA
TATGATGCATGATTCATGTCATGCTGCCATTTCACATTATCCAGGCGTATGGAAATGGAT
GGGCGCATCATTTGATTTCGTCACTGGTGCTTCATTCCTTTCATGGTGTCATCAACATGT
AATTGGTCATCA----------

>VFB864Z.Seq
----------TTGTCACTCCATTCCAAACTCGTTCATGGTATCATAAATATCAACACATT
TACGCTCCACTCCTCTATGGTATTTACACACTCAAATATCGTACTCAAGATTGGGAAGCT
TTTGTAAAGGATGGTAAAAATGGTGCAATTCGTGTTAGTGTCGCCACAAATTTCGATAAG
GCCGCTTACGTCATTGGTAAATTGTCTTTTGTTTTCTTCCGTTTCATCCTTCCACTCCGT
TATCATAGCTTTACAGATTTAATTTGTTATTTCCTCATTGCTGAATTCGTCTTTGGTTGG
TATCTCACAATTAATTTCCAAGTTAGTCATGTCGCTGAAGATCTCAAATTCTTTGCTACC
CCTGAAAGACCAGATGAACCATCTCAAATCAATGAAGATTGGGCAATCCTTCAACTTAAA
ACTACTCAAGATTATGGTCATGGTTCACTCCTTTGTACCTTTTTTAGTGGTTCTTTAAAT
CATCAAGTTGTTCATCATTTATTCCCATCAATTGCTCAAGATTTCTACCCACAACTTGTA
CCAATTGTAAAAGAAGTTTGTAAAGAACATAACATTACTTACCACATTAAACCAAACTTC
ACTGAAGCTATTATGTCACACATTAATTACCTTTACAAAATGGGTAATGATCCAGATTAT
GTTAAAAAACCATAGCCTCAAAAGA

>VFB864P.Seq
GTAGTATTTTCTGTACATATATTAATAGAATTGTAATAAAATGTCAAAAGTAATTACAGG
AAAGCAATACTCTTGGTCAGAGTTAGCCAAACATAACACTGAAAATGATTGTTGGGTTGC
AGTCGATGGTAAAGTTTATGATATTACAAGATGGGTACCATTACATCCAGGTGGTAAAGA
AGTTTTACTTTTAGCAGCAGGTAGAGATGTTACAAATTTATTTGAAAGTTATCATCCAAT
GTCAGATAAACCAACATCAATTTTAAAGAATTATGAAATTGGATATATTTCATCATATGA
ACATCCAAAATTTGTTCAAAAATCAGATTTCTACAAGACATTAAAAGAGAGAGTTAGAAA
ACATTTTAAAGCAACCGATCAAGATCCACAAATGGCAGTTAGTATTTTCTCACGTTTAGC
ATTGGTTTATCTTTTAGTGTTTGTAACTTACTATTTAGCACATTATACCAGTAACAATTT
TTATTTAAATTGTTTCTTGGCAATCGTTTACGCACTTTGCAACTCATTATTCTCAATGCA
TATGATGCATGATTCATGTCATGCTGCCATTTCACATTATCCAGGCGTATGGAAATGGAT
GGGCGCATCATTTGATTTCGTCACTGGTGCTTCATTCCTTTCATGGTGTCATCAACATGT
AATTGGTCATCA----------TTGTCACTCCATTCCAAACTCGTTCATGGTATCATAAA
TATCAACACATTTACGCTCCACTCCTCTATGGTATTTACACACTCAAATATCGTACTCAA
GATTGGGAAGCTTTTGTAAAGGATGGTAAAAATGGTGCAATTCGTGTTAGTGTCGCCACA
AATTTCGATAAGGCCGCTTACGTCATTGGTAAATTGTCTTTTGTTTTCTTCCGTTTCATC
CTTCCACTCCGTTATCATAGCTTTACAGATTTAATTTGTTATTTCCTCATTGCTGAATTC
GTCTTTGGTTGGTATCTCACAATTAATTTCCAAGTTAGTCATGTCGCTGAAGATCTCAAA
TTCTTTGCTACCCCTGAAAGACCAGATGAACCATCTCAAATCAATGAAGATTGGGCAATC
CTTCAACTTAAAACTACTCAAGATTATGGTCATGGTTCACTCCTTTGTACCTTTTTTAGT
GGTTCTTTAAATCATCAAGTTGTTCATCATTTATTCCCATCAATTGCTCAAGATTTCTAC
CCACAACTTGTACCAATTGTAAAAGAAGTTTGTAAAGAACATAACATTACTTACCACATT
AAACCAAACTTCACTGAAGCTATTATGTCACACATTAATTACCTTTACAAAATGGGTAAT
GATCCAGATTATGTTAAAAAACCATAGCCTCAAAAGA