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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFB862
Library VF
(Link to library)
Clone ID VFB862
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16311-1
Original site URL
Representative seq. ID VFB862P
(Link to Original site)
Representative DNA sequence 
>VFB862 (VFB862Q) /CSM/VF/VFB8-C/VFB862Q.Seq.d/
ATATAAATAAAATGAAAGTTTTATCTGCCTTATGCGTTTTATTAGTCAGTGTTGCCACTG
CCAAACAACAATTATCCGAAGTTGAATACAGAAATGCTTTCACCAATTGGATGATTGCTC
ACCAAAGACATTATTCATCAGAAGAGTTCAATGGTCGTTACAATATCTTCAAAGCCAATA
TGGATTACGTCAATGAATGGAATACCAAAGGTAGTGAAACCGTTTTAGGTTTAAATGTCT
TTGCTGATATCAGCAATGAAGAATACAGAGCCACCTATTTAGGTACCCCATTCGATGCTT
CATCCCTCGAAATGACCGAATCCGATAAAATCTTCGATGCTTCCGCTCAAGTCGATTGGA
GAACTCAAGGTGCTGTCACCCCAATCAAAAATCAAGGTCAATGTGGTGGTTGTTGGTCAT
TCTCAACTACTGGTGCCACTGAAGGTGCTCAATACCTTGCCAACGGTAAAAAGAATTTAG
TCTCATTATCCGAACAAAATTTAATCGATTGTTCAGGTTCATACGGTAACAACGGTTGTG
AAGGTGGTTTAATGACCTTAGCTTTCGAATACATCATCAATAACAAAGGTATTGATACTG
AAAGCAGCTACCCCATACACTGCTXXXXXXXXXXTGCCATTGATGCATCCAACCAATCAT
TCCAATTGTACGTTTCAGGTATCTACAATGAGCCAGCTTGCTCTTCAACTCAATTAGATC
ACGGTGTCTTAGCTGTTGGTTTCGGTACTGGTAGTGGTTCATCTGGTTCCCATGGTGGTT
CCCAATCACAATCTGCTGGTTCAGATTCACAATCTGCTGGTTCAGAATCATCCCAATCTG
AATCCGGTTCACAATCACAATCTGAATCCGGTTCACAATCACAATCGCAATCTGGTTCTC
AATCATTCTCTGGTTCCTTATACTCTGGTTCATACTCTGGTTCCCAATCTGGTTCCCAAT
CTGGTAACAGCGGTGCTGCTGTTAAACAAACTGGTGCTGGTTCAGGTTCAGGTTCAGGTT
CAGGTTCCGGTTCCGGTTCAGGTTCAGGTTCAGTCTCAGGTTCAGCTTCAGGTTCCGCCT
CAGGTTCCGCCTCAGGTTCATCAAGTGGTAGCAATAGCAACGGTGGTGTTTATCCAACCG
CTGGTGACTACTGGATCGTTAAAAACTCATGGGGTACCTCATGGGGTATGGATGGTTACA
TTTTAATGACCAAAGGAAATAACAATCAATGCGGTATTGCAACAATGGCCTCACGTCCAA
CTGCTGTTGCATCACTTTAAATTAACGATACATATATATACATATTTATATAATTTTATA
ATTAATAAAAAATAATACGAAAACATATAAAATT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
INKMKVLSALCVLLVSVATAKQQLSEVEYRNAFTNWMIAHQRHYSSEEFNGRYNIFKANM
DYVNEWNTKGSETVLGLNVFADISNEEYRATYLGTPFDASSLEMTESDKIFDASAQVDWR
TQGAVTPIKNQGQCGGCWSFSTTGATEGAQYLANGKKNLVSLSEQNLIDCSGSYGNNGCE
GGLMTLAFEYIINNKGIDTESSYPIHC---

---AIDASNQSFQLYVSGIYNEPACSSTQLDHGVLAVGFGTGSGSSGSHGGSQSQSAGSD
SQSAGSESSQSESGSQSQSESGSQSQSQSGSQSFSGSLYSGSYSGSQSGSQSGNSGAAVK
QTGAGSGSGSGSGSGSGSGSGSVSGSASGSASGSASGSSSGSNSNGGVYPTAGDYWIVKN
SWGTSWGMDGYILMTKGNNNQCGIATMASRPTAVASL*indtyiyifi*fyn**kiirkh
ik
Translated Amino Acid sequence (All Frames) 
Frame A:
i*ik*kfylpyafy*svlplpnnnypklntemlspig*lltkdiihqkssmvvtisskpi
witsmngipkvvkpf*v*msllisamknteppi*vphsmlhpsk*pnpikssmlplksig
elkvlspqskikvnvvvvghsqllvplkvlntlptvkri*shypnki*sivqvhtvttvv
kvv**p*lsntssitkvlilkaatpyta---

---ch*ciqpiipivrfrylq*asllfnsirsrclscwfryw*wfiwfpwwfpiticwfr
fticwfriipi*irftiti*irftitiaiwfsiilwflilwfilwfpiwfpiw*qrccc*
tnwcwfrfrfrfrfrfrfrfrfslrfsfrfrlrfrlrfikw*q*qrwclsnrw*lldr*k
lmgylmgygwlhfndqrk*qsmrycnngltsncccitln*ryiyihiyiil*liknntkt
yki

Frame B:
yk*nesficlmrfisqcchcqttiirs*iqkcfhqlddcspktlfirrvqwslqylqsqy
glrq*meyqr**nrfrfkclc*yqq*riqshlfrypircfiprndrir*nlrcfrssrle
nsrcchpnqksrsmwwllvilnywch*rcsipcqr*kefsliirtkfnrlfrfir*qrl*
rwfndlsfrihhq*qry*y*kqlphtl---

---AIDASNQSFQLYVSGIYNEPACSSTQLDHGVLAVGFGTGSGSSGSHGGSQSQSAGSD
SQSAGSESSQSESGSQSQSESGSQSQSQSGSQSFSGSLYSGSYSGSQSGSQSGNSGAAVK
QTGAGSGSGSGSGSGSGSGSGSVSGSASGSASGSASGSSSGSNSNGGVYPTAGDYWIVKN
SWGTSWGMDGYILMTKGNNNQCGIATMASRPTAVASL*indtyiyifi*fyn**kiirkh
ik

Frame C:
INKMKVLSALCVLLVSVATAKQQLSEVEYRNAFTNWMIAHQRHYSSEEFNGRYNIFKANM
DYVNEWNTKGSETVLGLNVFADISNEEYRATYLGTPFDASSLEMTESDKIFDASAQVDWR
TQGAVTPIKNQGQCGGCWSFSTTGATEGAQYLANGKKNLVSLSEQNLIDCSGSYGNNGCE
GGLMTLAFEYIINNKGIDTESSYPIHC---

---plmhptnhsnctfqvstmsqlalqln*itvs*llvsvlvvvhlvpmvvpnhnllvqi
hnllvqnhpnlnpvhnhnlnpvhnhnrnlvlnhslvpytlvhtlvpnlvpnlvtavllln
klvlvqvqvqvqvpvpvqvqvqsqvqlqvppqvppqvhqvvaiatvvfiqplvttgslkt
hgvphgvwmvtf**pkeitinavlqqwphvqlllhhfkltihiytylynfiinkk*yeni
*n
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFB862 (VFB862Q) /CSM/VF/VFB8-C/VFB862Q.Seq.d/                       1919   0.0
VFL437 (VFL437Q) /CSM/VF/VFL4-B/VFL437Q.Seq.d/                       1221   0.0
VFK358 (VFK358Q) /CSM/VF/VFK3-C/VFK358Q.Seq.d/                       1221   0.0
VFI407 (VFI407Q) /CSM/VF/VFI4-A/VFI407Q.Seq.d/                       1221   0.0
VFF871 (VFF871Q) /CSM/VF/VFF8-C/VFF871Q.Seq.d/                       1221   0.0
VFI460 (VFI460Q) /CSM/VF/VFI4-C/VFI460Q.Seq.d/                       1217   0.0
VFL517 (VFL517Q) /CSM/VF/VFL5-A/VFL517Q.Seq.d/                       1215   0.0
VHB683 (VHB683Q) /CSM/VH/VHB6-D/VHB683Q.Seq.d/                       1213   0.0
VFN428 (VFN428Q) /CSM/VF/VFN4-B/VFN428Q.Seq.d/                       1213   0.0
VFK835 (VFK835Q) /CSM/VF/VFK8-B/VFK835Q.Seq.d/                       1213   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

U72746|U72746.1 Dictyostelium discoideum cysteine proteinase (cprG) mRNA, complete cds.  1209  0.0  5
U72745|U72745.1 Dictyostelium discoideum cysteine proteinase (cprF) mRNA, complete cds.   484  0.0  10
L36204|L36204.1 Dictyostelium discoideum cysteine proteinase (CP4) mRNA, complete cds.   131  e-141  9
L36205|L36205.1 Dictyostelium discoideum cysteine proteinase CP5 mRNA, complete cds.   147  e-134  8
AC117072|AC117072.2 Dictyostelium discoideum chromosome 2 map 3323568-3470138 strain AX4, complete sequence.   153  e-119  10
X03344|X03344.1 Dictyostelium discoideum mRNA for cysteine proteinase 2.    92  1e-35  4
M16039|M16039.1 Dictyostelium discoideum pst-cath gene encoding pst-cathepsin, complete cds.    72  1e-27  5
AJ489298|AJ489298.1 Aphis gossypii mRNA for putative cathepsin L (catL gene).    48  4e-14  4
X02407|X02407.1 D.discoideum mRNA for cysteine proteinase 1.      90  9e-14  1
AJ496197|AJ496197.1 Myzus persicae mRNA for putative cathepsin L (catL gene).    48  3e-12  3
dna update 2003. 9.10
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q94504) RecName: Full=Cysteine proteinase 7;          EC=3.4.22...   441   e-122
(Q94503) RecName: Full=Cysteine proteinase 6;          EC=3.4.22...   386   e-106
L36204_1(L36204|pid:none) Dictyostelium discoideum cysteine prot...   355   2e-96
(P54640) RecName: Full=Cysteine proteinase 5;          EC=3.4.22...   324   4e-87
L36205_1(L36205|pid:none) Dictyostelium discoideum cysteine prot...   319   2e-85
(P04989) RecName: Full=Cysteine proteinase 2;          EC=3.4.22...   270   7e-71
EF053509_1(EF053509|pid:none) Acanthamoeba castellanii cysteine ...   234   5e-60
AY220615_1(AY220615|pid:none) Hydra vulgaris cathepsin L precurs...   207   5e-52
AC117076_20(AC117076|pid:none) Dictyostelium discoideum chromoso...   207   7e-52
EU143709_1(EU143709|pid:none) Stichopus japonicus cathepsin L mR...   199   1e-49
protein update 2009. 6.17
PSORT 

 psg: 0.95  gvh: 0.81  alm: 0.50  top: 0.33  tms: 0.00  mit: 0.30  mip: 0.07
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 60.0 %: extracellular, including cell wall
	 12.0 %: mitochondrial
	 12.0 %: vacuolar
	  8.0 %: endoplasmic reticulum
	  4.0 %: cytoplasmic
	  4.0 %: nuclear

>> prediction for VFB862 is exc
5' end seq. ID VFB862F
5' end seq. 
>VFB862F.Seq
ATATAAATAAAATGAAAGTTTTATCTGCCTTATGCGTTTTATTAGTCAGTGTTGCCACTG
CCAAACAACAATTATCCGAAGTTGAATACAGAAATGCTTTCACCAATTGGATGATTGCTC
ACCAAAGACATTATTCATCAGAAGAGTTCAATGGTCGTTACAATATCTTCAAAGCCAATA
TGGATTACGTCAATGAATGGAATACCAAAGGTAGTGAAACCGTTTTAGGTTTAAATGTCT
TTGCTGATATCAGCAATGAAGAATACAGAGCCACCTATTTAGGTACCCCATTCGATGCTT
CATCCCTCGAAATGACCGAATCCGATAAAATCTTCGATGCTTCCGCTCAAGTCGATTGGA
GAACTCAAGGTGCTGTCACCCCAATCAAAAATCAAGGTCAATGTGGTGGTTGTTGGTCAT
TCTCAACTACTGGTGCCACTGAAGGTGCTCAATACCTTGCCAACGGTAAAAAGAATTTAG
TCTCATTATCCGAACAAAATTTAATCGATTGTTCAGGTTCATACGGTAACAACGGTTGTG
AAGGTGGTTTAATGACCTTAGCTTTCGAATACATCATCAATAACAAAGGTATTGATACTG
AAAGCAGCTACCCCATACACTGCT----------
Length of 5' end seq. 624
3' end seq. ID VFB862Z
3' end seq. 
>VFB862Z.Seq
----------TGCCATTGATGCATCCAACCAATCATTCCAATTGTACGTTTCAGGTATCT
ACAATGAGCCAGCTTGCTCTTCAACTCAATTAGATCACGGTGTCTTAGCTGTTGGTTTCG
GTACTGGTAGTGGTTCATCTGGTTCCCATGGTGGTTCCCAATCACAATCTGCTGGTTCAG
ATTCACAATCTGCTGGTTCAGAATCATCCCAATCTGAATCCGGTTCACAATCACAATCTG
AATCCGGTTCACAATCACAATCGCAATCTGGTTCTCAATCATTCTCTGGTTCCTTATACT
CTGGTTCATACTCTGGTTCCCAATCTGGTTCCCAATCTGGTAACAGCGGTGCTGCTGTTA
AACAAACTGGTGCTGGTTCAGGTTCAGGTTCAGGTTCAGGTTCCGGTTCCGGTTCAGGTT
CAGGTTCAGTCTCAGGTTCAGCTTCAGGTTCCGCCTCAGGTTCCGCCTCAGGTTCATCAA
GTGGTAGCAATAGCAACGGTGGTGTTTATCCAACCGCTGGTGACTACTGGATCGTTAAAA
ACTCATGGGGTACCTCATGGGGTATGGATGGTTACATTTTAATGACCAAAGGAAATAACA
ATCAATGCGGTATTGCAACAATGGCCTCACGTCCAACTGCTGTTGCATCACTTTAAATTA
ACGATACATATATATACATATTTATATAATTTTATAATTAATAAAAAATAATACGAAAAC
ATATAAAATT
Length of 3' end seq. 720
Connected seq. ID VFB862P
Connected seq. 
>VFB862P.Seq
ATATAAATAAAATGAAAGTTTTATCTGCCTTATGCGTTTTATTAGTCAGTGTTGCCACTG
CCAAACAACAATTATCCGAAGTTGAATACAGAAATGCTTTCACCAATTGGATGATTGCTC
ACCAAAGACATTATTCATCAGAAGAGTTCAATGGTCGTTACAATATCTTCAAAGCCAATA
TGGATTACGTCAATGAATGGAATACCAAAGGTAGTGAAACCGTTTTAGGTTTAAATGTCT
TTGCTGATATCAGCAATGAAGAATACAGAGCCACCTATTTAGGTACCCCATTCGATGCTT
CATCCCTCGAAATGACCGAATCCGATAAAATCTTCGATGCTTCCGCTCAAGTCGATTGGA
GAACTCAAGGTGCTGTCACCCCAATCAAAAATCAAGGTCAATGTGGTGGTTGTTGGTCAT
TCTCAACTACTGGTGCCACTGAAGGTGCTCAATACCTTGCCAACGGTAAAAAGAATTTAG
TCTCATTATCCGAACAAAATTTAATCGATTGTTCAGGTTCATACGGTAACAACGGTTGTG
AAGGTGGTTTAATGACCTTAGCTTTCGAATACATCATCAATAACAAAGGTATTGATACTG
AAAGCAGCTACCCCATACACTGCT----------TGCCATTGATGCATCCAACCAATCAT
TCCAATTGTACGTTTCAGGTATCTACAATGAGCCAGCTTGCTCTTCAACTCAATTAGATC
ACGGTGTCTTAGCTGTTGGTTTCGGTACTGGTAGTGGTTCATCTGGTTCCCATGGTGGTT
CCCAATCACAATCTGCTGGTTCAGATTCACAATCTGCTGGTTCAGAATCATCCCAATCTG
AATCCGGTTCACAATCACAATCTGAATCCGGTTCACAATCACAATCGCAATCTGGTTCTC
AATCATTCTCTGGTTCCTTATACTCTGGTTCATACTCTGGTTCCCAATCTGGTTCCCAAT
CTGGTAACAGCGGTGCTGCTGTTAAACAAACTGGTGCTGGTTCAGGTTCAGGTTCAGGTT
CAGGTTCCGGTTCCGGTTCAGGTTCAGGTTCAGTCTCAGGTTCAGCTTCAGGTTCCGCCT
CAGGTTCCGCCTCAGGTTCATCAAGTGGTAGCAATAGCAACGGTGGTGTTTATCCAACCG
CTGGTGACTACTGGATCGTTAAAAACTCATGGGGTACCTCATGGGGTATGGATGGTTACA
TTTTAATGACCAAAGGAAATAACAATCAATGCGGTATTGCAACAATGGCCTCACGTCCAA
CTGCTGTTGCATCACTTTAAATTAACGATACATATATATACATATTTATATAATTTTATA
ATTAATAAAAAATAATACGAAAACATATAAAATT
Length of connected seq. 1344
Full length Seq ID -
Full length Seq. -
Length of full length seq. -