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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFB856
Library VF
(Link to library)
Clone ID VFB856
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16605-1
Original site URL
Representative seq. ID VFB856P
(Link to Original site)
Representative DNA sequence 
>VFB856 (VFB856Q) /CSM/VF/VFB8-C/VFB856Q.Seq.d/
ACCATCAATTAAATATATATATACTAAAAATGAGAGTTTTATCATTCCTTTGTTTATTAT
TAGTTAGCTACGCTTCTGCTAAACAACAATTCTCTGAATTACAATACAGAAATGCTTTCA
CCAACTGGATGCAAGCTCACCAAAGAACTTATTCCTCTGAAGAATTTAATGCTCGTTATC
AAATCTTCAAATCCAATATGGATTATGTACACCAATGGAATTCAAAAGGTGGTGAAACCG
TTTTGGGTTTAAATGTTTTCGCTGATATTACCAACCAAGAATATAGAACTACCTACTTGG
GTACCCCATTCGATGGTTCAGCCCTCATTGGTACTGAAGAAGAGAAAATCTTCTCCACCC
CAGCCCCAACTGTTGATTGGAGAGCTCAAGGTGCTGTCACACCAATTAAAAATCAAGGTC
AATGTGGTGGCTGCTGGTCATTCTCAACCACTGGTTCAACTGAAGGTGCTCACTTTATTG
CATCTGGAACAAAAAAAGATTTAGTTTCATTATCTGAACAAAACTTGATCGATTGTTCAA
AATCATACGGTAXXXXXXXXXXTGGTTATGGTTCAGGTTCAAGTTCATCATCTGGTTCAT
CATCTGGTAAATCATCATCATCATCATCAGGCTGGGTGGTAAAACTTCATCCTCATCATC
ATCAGGTAAAGCTTCATCATCATCATCAGGCAAAGCTTCATCATCATCATCATCAGGTAA
AACTTCATCTGCTGCTTCATCAACCTCTGGTTCTCAATCAGGTTCCCAATCAGGTAGCCA
ATCAGGCCAATCCACCGGTTCACAATCAGGTCAAACCTCTGCTTCTGGTCAAGCATCAGC
ATCAGGTTCTGGTTCTGGCTCAGGTTCAGGTTCAGGTTCAGGTTCAGGCTCAGGTGCTGT
TGAGGCCTCATCTGGTAACTACTGGATCGTTAAAAACTCATGGGGTACTTCATGGGGTAT
GGATGGTTACATTTTTATGAGCAAAGATAGAAATAACAATTGTGGTATCGCAACAATGGC
TTCTTTCCCAACTGCCTCATCAAATTAAAATTTTATTTTTAATTGTGCCGACTAACATTA
ATTNGNATTTGTAATAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
HQLNIYILKMRVLSFLCLLLVSYASAKQQFSELQYRNAFTNWMQAHQRTYSSEEFNARYQ
IFKSNMDYVHQWNSKGGETVLGLNVFADITNQEYRTTYLGTPFDGSALIGTEEEKIFSTP
APTVDWRAQGAVTPIKNQGQCGGCWSFSTTGSTEGAHFIASGTKKDLVSLSEQNLIDCSK
SYG---

---GYGSGSSSSSGSSSGKSSSSSSGWVVKLHPHHHQVKLHHHHQAKLHHHHHQVKLHLL
LHQPLVLNQVPNQVANQANPPVHNQVKPLLLVKHQHQVLVLAQVQVQVQVQAQVLLRPHL
VTTGSLKTHGVLHGVWMVTFL*akieitivvsqqwllsqlphqikilflivptninxxl*
*
Translated Amino Acid sequence (All Frames) 
Frame A:
tin*iyiy*k*efyhsfvyy*latlllnnnslnyntemlsptgckltkeliplknlmlvi
kssnpiwimytngiqkvvkpfwv*mfslilptknielptwvphsmvqpslvlkkrksspp
qpqlligelkvlshqlkikvnvvaaghsqplvqlkvltllhleqkki*fhylnkt*sivq
nhtv---

---wlwfrfkfiiwfiiw*iiiiiirlggktssssssgkasssssgkassssssgktssa
asstsgsqsgsqsgsqsgqstgsqsgqtsasgqasasgsgsgsgsgsgsgsgsgaveass
gnywivknswgtswgmdgyifmskdrnnncgiatmasfptassn*nfifncad*h*xxfv
i

Frame B:
psikyiytknesfiiplfiis*lrfc*ttil*itiqkcfhqldasspknlfl*ri*csls
nlqiqyglctpmefkrw*nrfgfkcfr*yyqpri*nyllgypirwfsphwy*rrenllhp
spnc*lessrcchtn*ksrsmwwllvilnhwfn*rcslyciwnkkrfsfii*tkldrlfk
iir---

---GYGSGSSSSSGSSSGKSSSSSSGWVVKLHPHHHQVKLHHHHQAKLHHHHHQVKLHLL
LHQPLVLNQVPNQVANQANPPVHNQVKPLLLVKHQHQVLVLAQVQVQVQVQAQVLLRPHL
VTTGSLKTHGVLHGVWMVTFL*akieitivvsqqwllsqlphqikilflivptninxxl*
*

Frame C:
HQLNIYILKMRVLSFLCLLLVSYASAKQQFSELQYRNAFTNWMQAHQRTYSSEEFNARYQ
IFKSNMDYVHQWNSKGGETVLGLNVFADITNQEYRTTYLGTPFDGSALIGTEEEKIFSTP
APTVDWRAQGAVTPIKNQGQCGGCWSFSTTGSTEGAHFIASGTKKDLVSLSEQNLIDCSK
SYG---

---vmvqvqvhhlvhhlvnhhhhhqagw*nfiliiir*sfiiiirqsfiiiiir*nficc
finlwfsirfpir*pirpihrftirsnlcfwssisirfwfwlrfrfrfrfrlrcc*gliw
*lldr*klmgyfmgygwlhfyeqr*k*qlwyrnngffpncliklkfyf*lcrltlixicn
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFO306 (VFO306Q) /CSM/VF/VFO3-A/VFO306Q.Seq.d/                       1053   0.0
VFM544 (VFM544Q) /CSM/VF/VFM5-B/VFM544Q.Seq.d/                       1053   0.0
VFM320 (VFM320Q) /CSM/VF/VFM3-A/VFM320Q.Seq.d/                       1053   0.0
VFK619 (VFK619Q) /CSM/VF/VFK6-A/VFK619Q.Seq.d/                       1053   0.0
VFK603 (VFK603Q) /CSM/VF/VFK6-A/VFK603Q.Seq.d/                       1053   0.0
VFK133 (VFK133Q) /CSM/VF/VFK1-B/VFK133Q.Seq.d/                       1053   0.0
VFG572 (VFG572Q) /CSM/VF/VFG5-C/VFG572Q.Seq.d/                       1053   0.0
VFG473 (VFG473Q) /CSM/VF/VFG4-D/VFG473Q.Seq.d/                       1053   0.0
VFG110 (VFG110Q) /CSM/VF/VFG1-A/VFG110Q.Seq.d/                       1053   0.0
VFF738 (VFF738Q) /CSM/VF/VFF7-B/VFF738Q.Seq.d/                       1053   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

L36204|L36204.1 Dictyostelium discoideum cysteine proteinase (CP4) mRNA, complete cds.   922  0.0  6
U72746|U72746.1 Dictyostelium discoideum cysteine proteinase (cprG) mRNA, complete cds.   131  e-108  7
L36205|L36205.1 Dictyostelium discoideum cysteine proteinase CP5 mRNA, complete cds.   121  e-102  6
U72745|U72745.1 Dictyostelium discoideum cysteine proteinase (cprF) mRNA, complete cds.   115  2e-95  7
AC117072|AC117072.2 Dictyostelium discoideum chromosome 2 map 3323568-3470138 strain AX4, complete sequence.   121  1e-93  8
X03344|X03344.1 Dictyostelium discoideum mRNA for cysteine proteinase 2.    76  1e-31  5
M16039|M16039.1 Dictyostelium discoideum pst-cath gene encoding pst-cathepsin, complete cds.    64  1e-25  7
X02407|X02407.1 D.discoideum mRNA for cysteine proteinase 1.      70  2e-12  2
AJ517568|AJ517568.1 Dreissena polymorpha EST, clone atrado65.     68  3e-07  1
CB398740|CB398740.1 OSTR208E2_2 AD-wrmcDNA Caenorhabditis elegans cDNA, mRNA sequence.    54  8e-06  2
dna update 2003. 9.10
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

L36204_1(L36204|pid:none) Dictyostelium discoideum cysteine prot...   360   5e-98
(Q94504) RecName: Full=Cysteine proteinase 7;          EC=3.4.22...   275   3e-72
(P54640) RecName: Full=Cysteine proteinase 5;          EC=3.4.22...   273   6e-72
L36205_1(L36205|pid:none) Dictyostelium discoideum cysteine prot...   267   6e-70
(Q94503) RecName: Full=Cysteine proteinase 6;          EC=3.4.22...   265   2e-69
(P04989) RecName: Full=Cysteine proteinase 2;          EC=3.4.22...   194   6e-48
EF053509_1(EF053509|pid:none) Acanthamoeba castellanii cysteine ...   156   1e-36
AC117076_20(AC117076|pid:none) Dictyostelium discoideum chromoso...   152   2e-35
AK226753_1(AK226753|pid:none) Arabidopsis thaliana mRNA for papa...   137   9e-31
AC000132_24(AC000132|pid:none) Sequence of BAC F21M12 from Arabi...   137   9e-31
protein update 2009. 6.17
PSORT 

 psg: 0.96  gvh: 0.87  alm: 0.42  top: 0.37  tms: 0.00  mit: 0.33  mip: 0.08
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 72.0 %: extracellular, including cell wall
	  8.0 %: mitochondrial
	  8.0 %: vacuolar
	  8.0 %: endoplasmic reticulum
	  4.0 %: cytoplasmic

>> prediction for VFB856 is exc
5' end seq. ID VFB856F
5' end seq. 
>VFB856F.Seq
ACCATCAATTAAATATATATATACTAAAAATGAGAGTTTTATCATTCCTTTGTTTATTAT
TAGTTAGCTACGCTTCTGCTAAACAACAATTCTCTGAATTACAATACAGAAATGCTTTCA
CCAACTGGATGCAAGCTCACCAAAGAACTTATTCCTCTGAAGAATTTAATGCTCGTTATC
AAATCTTCAAATCCAATATGGATTATGTACACCAATGGAATTCAAAAGGTGGTGAAACCG
TTTTGGGTTTAAATGTTTTCGCTGATATTACCAACCAAGAATATAGAACTACCTACTTGG
GTACCCCATTCGATGGTTCAGCCCTCATTGGTACTGAAGAAGAGAAAATCTTCTCCACCC
CAGCCCCAACTGTTGATTGGAGAGCTCAAGGTGCTGTCACACCAATTAAAAATCAAGGTC
AATGTGGTGGCTGCTGGTCATTCTCAACCACTGGTTCAACTGAAGGTGCTCACTTTATTG
CATCTGGAACAAAAAAAGATTTAGTTTCATTATCTGAACAAAACTTGATCGATTGTTCAA
AATCATACGGTA----------
Length of 5' end seq. 552
3' end seq. ID VFB856Z
3' end seq. 
>VFB856Z.Seq
----------TGGTTATGGTTCAGGTTCAAGTTCATCATCTGGTTCATCATCTGGTAAAT
CATCATCATCATCATCAGGCTGGGTGGTAAAACTTCATCCTCATCATCATCAGGTAAAGC
TTCATCATCATCATCAGGCAAAGCTTCATCATCATCATCATCAGGTAAAACTTCATCTGC
TGCTTCATCAACCTCTGGTTCTCAATCAGGTTCCCAATCAGGTAGCCAATCAGGCCAATC
CACCGGTTCACAATCAGGTCAAACCTCTGCTTCTGGTCAAGCATCAGCATCAGGTTCTGG
TTCTGGCTCAGGTTCAGGTTCAGGTTCAGGTTCAGGCTCAGGTGCTGTTGAGGCCTCATC
TGGTAACTACTGGATCGTTAAAAACTCATGGGGTACTTCATGGGGTATGGATGGTTACAT
TTTTATGAGCAAAGATAGAAATAACAATTGTGGTATCGCAACAATGGCTTCTTTCCCAAC
TGCCTCATCAAATTAAAATTTTATTTTTAATTGTGCCGACTAACATTAATTNGNATTTGT
AATAA
Length of 3' end seq. 535
Connected seq. ID VFB856P
Connected seq. 
>VFB856P.Seq
ACCATCAATTAAATATATATATACTAAAAATGAGAGTTTTATCATTCCTTTGTTTATTAT
TAGTTAGCTACGCTTCTGCTAAACAACAATTCTCTGAATTACAATACAGAAATGCTTTCA
CCAACTGGATGCAAGCTCACCAAAGAACTTATTCCTCTGAAGAATTTAATGCTCGTTATC
AAATCTTCAAATCCAATATGGATTATGTACACCAATGGAATTCAAAAGGTGGTGAAACCG
TTTTGGGTTTAAATGTTTTCGCTGATATTACCAACCAAGAATATAGAACTACCTACTTGG
GTACCCCATTCGATGGTTCAGCCCTCATTGGTACTGAAGAAGAGAAAATCTTCTCCACCC
CAGCCCCAACTGTTGATTGGAGAGCTCAAGGTGCTGTCACACCAATTAAAAATCAAGGTC
AATGTGGTGGCTGCTGGTCATTCTCAACCACTGGTTCAACTGAAGGTGCTCACTTTATTG
CATCTGGAACAAAAAAAGATTTAGTTTCATTATCTGAACAAAACTTGATCGATTGTTCAA
AATCATACGGTA----------TGGTTATGGTTCAGGTTCAAGTTCATCATCTGGTTCAT
CATCTGGTAAATCATCATCATCATCATCAGGCTGGGTGGTAAAACTTCATCCTCATCATC
ATCAGGTAAAGCTTCATCATCATCATCAGGCAAAGCTTCATCATCATCATCATCAGGTAA
AACTTCATCTGCTGCTTCATCAACCTCTGGTTCTCAATCAGGTTCCCAATCAGGTAGCCA
ATCAGGCCAATCCACCGGTTCACAATCAGGTCAAACCTCTGCTTCTGGTCAAGCATCAGC
ATCAGGTTCTGGTTCTGGCTCAGGTTCAGGTTCAGGTTCAGGTTCAGGCTCAGGTGCTGT
TGAGGCCTCATCTGGTAACTACTGGATCGTTAAAAACTCATGGGGTACTTCATGGGGTAT
GGATGGTTACATTTTTATGAGCAAAGATAGAAATAACAATTGTGGTATCGCAACAATGGC
TTCTTTCCCAACTGCCTCATCAAATTAAAATTTTATTTTTAATTGTGCCGACTAACATTA
ATTNGNATTTGTAATAA
Length of connected seq. 1087
Full length Seq ID -
Full length Seq. -
Length of full length seq. -