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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFB842
Library VF
(Link to library)
Clone ID VFB842
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U14191-1
Original site URL
Representative seq. ID VFB842P
(Link to Original site)
Representative DNA sequence 
>VFB842 (VFB842Q) /CSM/VF/VFB8-B/VFB842Q.Seq.d/
ATTCACCAAAATGCTTTCATTCAAATTCGCCAAATCTGCCTCAAAGGTTATTGGAAATAG
AAATTTCCACTCAAGTTCTGCTTCTTTAGCAAATACAACTAAATTATTTATTAATGGTAA
ATTTGTTGAATCAAAAACTAAAGAATGGCTCGAAGTTAGAAATCCAGCCACTCAAGAACT
TGTAACTAAAGTACCAGTTTCAACTAAAGAAGAGATGGAGGCCGCAGTTAAAGCAGCATC
AGATGCTTTCCCAGCATGGAGAGATACATCAGTTTCAAACAGATCAAGAATCATTAGCAA
CTATAAGAATTTAATCAATAAAAATATGGATAAAATTGCAGCAATCATTACAGAAGAACA
AGGTAAAACCCTCCCAGACGCAAAGGGTGATGTTTTCAGAGGTTTAGAAGTTGTTGAACA
TTCAGTCAATGTTGCATCATTAATGATGGGTGAAACCGTTGAAAATGTTTCAAAGAATGT
CGATATCTATTCATACGTTCAACCATTAGGTGTTTGTGCAGGTATCACTCCATTCAATTT
CCCAGCAATGATTCCATTATGGATGTTCCCATTAGCAATTGCATGTGGTAACACTTTTGT
TTTGAAACCXXXXXXXXXXGAACGTACTTTAGATGCATTGACTGGTGCTGCTTTTGGTGC
CTCTGGTCAACGTTGTATGGCTTTAAGTGCTGCTGTATTCGTTGGTGAATCTAAAAATTG
GATTCCAGAATTAGCTGAACGTGCCAAGAAATTAAAGGTTGCAGGTGGTGCTGCACCAGG
TGCTGATTTAGGTCCAGTGATTTCAGCAGCCTCAAAACAAAGAATTCACGAACTCATTCA
ATCTGGTATTGATGAAGGTGCCAAATGTATTTTAGATGGTCGTAACGTTGTCGTTGATCC
AGAATTTTCAAAGGGTAACTTTGTTGGTCCAACCATTCTCACTGATGTCAAACCACATAT
GAGATGTTACAAAGAGGAAATCTTTGGTCCAGTTTTAGTTTGTCTCAATGTTGATACTGT
CGATCAAGCCATTCAACTCATCAATGCTAACCCATACGGTAATGGTACTGCTGTCTTCAC
CTCATCTGGTGCTGTCGCTCGTAAATATCAACGTGAAATCGATGTTGGTCAAGTTGGTAT
CAATTTACCAATCCCAGTTCCACTTCCATTCTTCTCCTTCACTGGTTCTCGTGGTTCTTT
TGTTGGTGCTGGTCACTTCTACGGTAAGACTGGTGTTCAATTCTTTACTCAAATTAAAAC
AATCACTTCAAACTGGAGAGATGATGATATTTCACACGGTGTTTCTTCATCAATGAATAT
GCCAATTTTAGGTAAAAATTAAAAATTAATAATAAAAAAAAAAAAAAAAAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
FTKMLSFKFAKSASKVIGNRNFHSSSASLANTTKLFINGKFVESKTKEWLEVRNPATQEL
VTKVPVSTKEEMEAAVKAASDAFPAWRDTSVSNRSRIISNYKNLINKNMDKIAAIITEEQ
GKTLPDAKGDVFRGLEVVEHSVNVASLMMGETVENVSKNVDIYSYVQPLGVCAGITPFNF
PAMIPLWMFPLAIACGNTFVLK---

---ERTLDALTGAAFGASGQRCMALSAAVFVGESKNWIPELAERAKKLKVAGGAAPGADL
GPVISAASKQRIHELIQSGIDEGAKCILDGRNVVVDPEFSKGNFVGPTILTDVKPHMRCY
KEEIFGPVLVCLNVDTVDQAIQLINANPYGNGTAVFTSSGAVARKYQREIDVGQVGINLP
IPVPLPFFSFTGSRGSFVGAGHFYGKTGVQFFTQIKTITSNWRDDDISHGVSSSMNMPIL
GKN*kliikkkkk
Translated Amino Acid sequence (All Frames) 
Frame A:
ihqnafiqirqiclkgywk*kfplkfcffskyn*iiy*w*ic*ikn*rmars*ksshsrt
cn*stsfn*rrdggrs*ssircfpsmeryisfkqiknh*ql*efnq*kyg*ncsnhyrrt
r*npprrkg*cfqrfrsc*tfsqcciindg*nr*kcfkecrylfirstirclcryhsiqf
psndsimdvpisncmw*hfcfet---

---ERTLDALTGAAFGASGQRCMALSAAVFVGESKNWIPELAERAKKLKVAGGAAPGADL
GPVISAASKQRIHELIQSGIDEGAKCILDGRNVVVDPEFSKGNFVGPTILTDVKPHMRCY
KEEIFGPVLVCLNVDTVDQAIQLINANPYGNGTAVFTSSGAVARKYQREIDVGQVGINLP
IPVPLPFFSFTGSRGSFVGAGHFYGKTGVQFFTQIKTITSNWRDDDISHGVSSSMNMPIL
GKN*kliikkkkk

Frame B:
FTKMLSFKFAKSASKVIGNRNFHSSSASLANTTKLFINGKFVESKTKEWLEVRNPATQEL
VTKVPVSTKEEMEAAVKAASDAFPAWRDTSVSNRSRIISNYKNLINKNMDKIAAIITEEQ
GKTLPDAKGDVFRGLEVVEHSVNVASLMMGETVENVSKNVDIYSYVQPLGVCAGITPFNF
PAMIPLWMFPLAIACGNTFVLK---

---nvl*mh*lvlllvplvnvvwl*vllyslvnlkigfqn*lnvprn*rlqvvlhqvli*
vq*fqqpqnkeftnsfnlvlmkvpnvf*mvvtlsliqnfqrvtllvqpfslmsnhi*dvt
krkslvqf*fvsmlilsikpfnssmlthtvmvllssphlvlslvninvksmlvklvsiyq
sqfhfhsspslvlvvlllvlvtstvrlvfnsllklkqslqtgemmifhtvflhq*icqf*
vkikn***kkkkk

Frame C:
spkcfhsnspnlpqrlleieistqvlll*qiqlnyllmvnllnqklkngskleiqplknl
*lkyqfqlkkrwrpqlkqhqmlsqhgeihqfqtdqeslatiri*sikiwiklqqslqknk
vkpsqtqrvmfsev*kllniqsmlhh**wvkplkmfqrmsisihtfnh*vfvqvslhsis
qq*fhygcsh*qlhvvtllf*n---

---tyfrcidwccfwclwstlygfkcccirw*i*kldsris*tcqeikgcrwcctrc*fr
ssdfsslktknsrthsiwy**rcqmyfrws*rcr*srifkg*lcwsnhsh*cqttyemlq
rgnlwssfslsqc*ycrsshsthqc*pir*wycclhliwccrs*ist*nrcwsswyqftn
psstsilllhwfswffcwcwsllr*dwcsilysn*nnhfkler**yftrcffineyanfr
*klkinnkkkkkk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFB842 (VFB842Q) /CSM/VF/VFB8-B/VFB842Q.Seq.d/                       2623   0.0
VFI639 (VFI639Q) /CSM/VF/VFI6-B/VFI639Q.Seq.d/                       1455   0.0
CFG290 (CFG290Q) /CSM/CF/CFG2-D/CFG290Q.Seq.d/                       1447   0.0
VFF882 (VFF882Q) /CSM/VF/VFF8-D/VFF882Q.Seq.d/                       1443   0.0
VFE411 (VFE411Q) /CSM/VF/VFE4-A/VFE411Q.Seq.d/                       1443   0.0
VHB389 (VHB389Q) /CSM/VH/VHB3-D/VHB389Q.Seq.d/                       1437   0.0
VFC565 (VFC565Q) /CSM/VF/VFC5-C/VFC565Q.Seq.d/                       1388   0.0
VFG168 (VFG168Q) /CSM/VF/VFG1-C/VFG168Q.Seq.d/                       1364   0.0
SFA764 (SFA764Q) /CSM/SF/SFA7-C/SFA764Q.Seq.d/                       1261   0.0
VFK870 (VFK870Q) /CSM/VF/VFK8-C/VFK870Q.Seq.d/                       1233   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

CD230130|CD230130.1 SS1_41_C04.b1_A012 Salt-stressed seedlings Sorghum bicolor cDNA clone SS1_41_C04_A012 3', mRNA sequence.    82  2e-21  3
BM393668|BM393668.1 50072-2-10-C10.f.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.    52  4e-19  4
BM392567|BM392567.1 50071-2-10-C10.f.1 Chilcoat/Turkewitz cDNA (small fraction) Tetrahymena thermophila cDNA, mRNA sequence.    52  4e-19  4
AE015613|AE015613.1 Shewanella oneidensis MR-1 section 162 of 457 of the complete genome.    52  1e-14  5
BM396124|BM396124.1 5009-0-17-F02.t.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.    48  2e-10  3
BM398736|BM398736.1 5009-0-49-E06.t.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.    48  3e-10  3
AY255673|AY255673.1 Triticum aestivum methylmalonate semialdehyde dehydrogenase mRNA, partial cds; nuclear gene for mitochondrial product.    50  3e-10  3
AP005087|AP005087.1 Vibrio parahaemolyticus DNA, chromosome 2, complete sequence, 4/6.    76  3e-09  7
BM396340|BM396340.1 5009-0-2-E12.t.2 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.    48  6e-09  3
AF297860|AF297860.1 Mus musculus methylmalonate-semialdehyde dehydrogenase mRNA, complete cds.    44  2e-08  3
dna update 2003. 9.10
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q7QC84) RecName: Full=Probable methylmalonate-semialdehyde dehy...   321   3e-86
(Q29HB2) RecName: Full=Probable methylmalonate-semialdehyde dehy...   304   6e-81
BT031194_1(BT031194|pid:none) Drosophila melanogaster GH07313 fu...   302   2e-80
BC108615_1(BC108615|pid:none) Xenopus laevis hypothetical protei...   291   4e-77
BT072671_1(BT072671|pid:none) Salmo salar clone ssal-rgf-535-350...   288   4e-76
BT045940_1(BT045940|pid:none) Salmo salar clone ssal-rgf-537-096...   288   4e-76
(Q0WM29) RecName: Full=Methylmalonate-semialdehyde dehydrogenase...   285   3e-75
AK230004_1(AK230004|pid:none) Arabidopsis thaliana mRNA for F15N...   285   3e-75
(Q02253) RecName: Full=Methylmalonate-semialdehyde dehydrogenase...   285   3e-75
M93405_1(M93405|pid:none) Human methylmalonate semialdehyde dehy...   283   8e-75
protein update 2009. 6.17
PSORT 

 psg: 0.71  gvh: 0.38  alm: 0.35  top: 0.53  tms: 0.00  mit: 0.43  mip: 0.05
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: mitochondrial
	 20.0 %: cytoplasmic
	 16.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: extracellular, including cell wall
	  4.0 %: plasma membrane

>> prediction for VFB842 is mit
5' end seq. ID VFB842F
5' end seq. 
>VFB842F.Seq
ATTCACCAAAATGCTTTCATTCAAATTCGCCAAATCTGCCTCAAAGGTTATTGGAAATAG
AAATTTCCACTCAAGTTCTGCTTCTTTAGCAAATACAACTAAATTATTTATTAATGGTAA
ATTTGTTGAATCAAAAACTAAAGAATGGCTCGAAGTTAGAAATCCAGCCACTCAAGAACT
TGTAACTAAAGTACCAGTTTCAACTAAAGAAGAGATGGAGGCCGCAGTTAAAGCAGCATC
AGATGCTTTCCCAGCATGGAGAGATACATCAGTTTCAAACAGATCAAGAATCATTAGCAA
CTATAAGAATTTAATCAATAAAAATATGGATAAAATTGCAGCAATCATTACAGAAGAACA
AGGTAAAACCCTCCCAGACGCAAAGGGTGATGTTTTCAGAGGTTTAGAAGTTGTTGAACA
TTCAGTCAATGTTGCATCATTAATGATGGGTGAAACCGTTGAAAATGTTTCAAAGAATGT
CGATATCTATTCATACGTTCAACCATTAGGTGTTTGTGCAGGTATCACTCCATTCAATTT
CCCAGCAATGATTCCATTATGGATGTTCCCATTAGCAATTGCATGTGGTAACACTTTTGT
TTTGAAACC----------
Length of 5' end seq. 609
3' end seq. ID VFB842Z
3' end seq. 
>VFB842Z.Seq
----------GAACGTACTTTAGATGCATTGACTGGTGCTGCTTTTGGTGCCTCTGGTCA
ACGTTGTATGGCTTTAAGTGCTGCTGTATTCGTTGGTGAATCTAAAAATTGGATTCCAGA
ATTAGCTGAACGTGCCAAGAAATTAAAGGTTGCAGGTGGTGCTGCACCAGGTGCTGATTT
AGGTCCAGTGATTTCAGCAGCCTCAAAACAAAGAATTCACGAACTCATTCAATCTGGTAT
TGATGAAGGTGCCAAATGTATTTTAGATGGTCGTAACGTTGTCGTTGATCCAGAATTTTC
AAAGGGTAACTTTGTTGGTCCAACCATTCTCACTGATGTCAAACCACATATGAGATGTTA
CAAAGAGGAAATCTTTGGTCCAGTTTTAGTTTGTCTCAATGTTGATACTGTCGATCAAGC
CATTCAACTCATCAATGCTAACCCATACGGTAATGGTACTGCTGTCTTCACCTCATCTGG
TGCTGTCGCTCGTAAATATCAACGTGAAATCGATGTTGGTCAAGTTGGTATCAATTTACC
AATCCCAGTTCCACTTCCATTCTTCTCCTTCACTGGTTCTCGTGGTTCTTTTGTTGGTGC
TGGTCACTTCTACGGTAAGACTGGTGTTCAATTCTTTACTCAAATTAAAACAATCACTTC
AAACTGGAGAGATGATGATATTTCACACGGTGTTTCTTCATCAATGAATATGCCAATTTT
AGGTAAAAATTAAAAATTAATAATAAAAAAAAAAAAAAAAAA
Length of 3' end seq. 752
Connected seq. ID VFB842P
Connected seq. 
>VFB842P.Seq
ATTCACCAAAATGCTTTCATTCAAATTCGCCAAATCTGCCTCAAAGGTTATTGGAAATAG
AAATTTCCACTCAAGTTCTGCTTCTTTAGCAAATACAACTAAATTATTTATTAATGGTAA
ATTTGTTGAATCAAAAACTAAAGAATGGCTCGAAGTTAGAAATCCAGCCACTCAAGAACT
TGTAACTAAAGTACCAGTTTCAACTAAAGAAGAGATGGAGGCCGCAGTTAAAGCAGCATC
AGATGCTTTCCCAGCATGGAGAGATACATCAGTTTCAAACAGATCAAGAATCATTAGCAA
CTATAAGAATTTAATCAATAAAAATATGGATAAAATTGCAGCAATCATTACAGAAGAACA
AGGTAAAACCCTCCCAGACGCAAAGGGTGATGTTTTCAGAGGTTTAGAAGTTGTTGAACA
TTCAGTCAATGTTGCATCATTAATGATGGGTGAAACCGTTGAAAATGTTTCAAAGAATGT
CGATATCTATTCATACGTTCAACCATTAGGTGTTTGTGCAGGTATCACTCCATTCAATTT
CCCAGCAATGATTCCATTATGGATGTTCCCATTAGCAATTGCATGTGGTAACACTTTTGT
TTTGAAACC----------GAACGTACTTTAGATGCATTGACTGGTGCTGCTTTTGGTGC
CTCTGGTCAACGTTGTATGGCTTTAAGTGCTGCTGTATTCGTTGGTGAATCTAAAAATTG
GATTCCAGAATTAGCTGAACGTGCCAAGAAATTAAAGGTTGCAGGTGGTGCTGCACCAGG
TGCTGATTTAGGTCCAGTGATTTCAGCAGCCTCAAAACAAAGAATTCACGAACTCATTCA
ATCTGGTATTGATGAAGGTGCCAAATGTATTTTAGATGGTCGTAACGTTGTCGTTGATCC
AGAATTTTCAAAGGGTAACTTTGTTGGTCCAACCATTCTCACTGATGTCAAACCACATAT
GAGATGTTACAAAGAGGAAATCTTTGGTCCAGTTTTAGTTTGTCTCAATGTTGATACTGT
CGATCAAGCCATTCAACTCATCAATGCTAACCCATACGGTAATGGTACTGCTGTCTTCAC
CTCATCTGGTGCTGTCGCTCGTAAATATCAACGTGAAATCGATGTTGGTCAAGTTGGTAT
CAATTTACCAATCCCAGTTCCACTTCCATTCTTCTCCTTCACTGGTTCTCGTGGTTCTTT
TGTTGGTGCTGGTCACTTCTACGGTAAGACTGGTGTTCAATTCTTTACTCAAATTAAAAC
AATCACTTCAAACTGGAGAGATGATGATATTTCACACGGTGTTTCTTCATCAATGAATAT
GCCAATTTTAGGTAAAAATTAAAAATTAATAATAAAAAAAAAAAAAAAAAA
Length of connected seq. 1361
Full length Seq ID -
Full length Seq. -
Length of full length seq. -