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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFB838
Library VF
(Link to library)
Clone ID VFB838
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U14973-1
Original site URL
Representative seq. ID VFB838P
(Link to Original site)
Representative DNA sequence 
>VFB838 (VFB838Q) /CSM/VF/VFB8-B/VFB838Q.Seq.d/
ACATTCTTTCACAAACCAAAATAAAAAATGTTATCAAATTTTTTAAAAGTCAACAGTAAA
GCACTAGGACATATAAGAACTTTTGCCTCAAAGAGTGGTGAAATTAAACACAATTTTAAA
AAAGCCGATACATACTTATGTGATGGTCCATCTGACTCAACTGTTACCAACAAAGATGAA
CTCATTTCATTCTTCACTGAAATGTCAAGATTTAGAAGACTCGAAACTGTTTGTGATGGT
TTATACAAAAAAAAATTAATTAGAGGTTTCTGTCATTTATACACTGGTCAAGAAGCTGTT
TGTGCAGGTTTAGAAAGTGCAATTACTAAAGATGATCATATTATTACAGCATACAGAGAT
CACACTTATATGTTATCAAGAGGAGCAACACCAGAAGAAATTTTTGCAGAATTATTAATG
AAAGAAACTGGTTGTTCAAAGGGTAAAGGTGGTTCAATGCATATGTTTACAAAGAATTTC
TATGGTGGTAATGGTATTGTTGGTGCTCAATGTCCACTTTGGTGCTGGTATTGCCTTTGC
NCAAAAGTACAATAAGACCGGTXXXXXXXXXXACGATTTCTATANTCGTGGTCACTATGT
TGCAGGCTTAAAAGTCGATGGTATGGATGTTTTCGCCGTCAAAGAAGCTGGTAAATACGC
CGCTGAATGGTGTAGAGCTGGTAATGGTCCAATCATTTTANAAATGGATACCTATCGTTA
TGTAGGTCACTCTATGTNTGATCCAGGTATCACTTATCGTACAAGAGAAGAAGTTAACCA
CGTTAGACAAACTANAGATCCAATTGAAAACATTCGTCNAATCATCTTGGNCAATAAAAT
CGCAACTGAAGATCAACTCGCTNCCATCNAAGAAACCGTTANAGATGAAATGGAAAAAGC
CTCTGAAAAAGCTACCGCTGCTCCATTNCCACAAGCTCGTGAACTCTTTACCAACGTTTA
CCTCCAAGAAGTTCCAGTAAGAGGTGTTGAATTTGTTAACAGTTTCAAACCATAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
hsftnqnkkcyqif*kstvkh*di*ellpqrvvklntilkkpihtyvmvhltqllptkmn
sfhsslkcqdledsklfvmvytkkn*levsviytlvkklfvqv*kvqllkmiillqhtei
tlicyqeeqhqkkflqny**KKLVVQRVKVVQCICLQRISMVVMVLLVLNVHFGAGIAFA
QKYNKTG---

---DFYXRGHYVAGLKVDGMDVFAVKEAGKYAAEWCRAGNGPIILXMDTYRYVGHSMXDP
GITYRTREEVNHVRQTXDPIENIRXIILXNKIATEDQLAXIXETVXDEMEKASEKATAAP
XPQARELFTNVYLQEVPVRGVEFVNSFKP*
Translated Amino Acid sequence (All Frames) 
Frame A:
tffhkpk*kmlsnflkvnskalghirtfasksgeikhnfkkadtylcdgpsdstvtnkde
lisfftemsrfrrletvcdglykkklirgfchlytgqeavcaglesaitkddhiitayrd
htymlsrgatpeeifaellmketgcskgkggsmhmftknfyggngivgaqcplwcwyclc
xkvq*dr---

---tisixvvtmlqa*ksmvwmfspskklvntplngvelvmvqsfxkwipivm*vtlcxi
qvslivqekklttldklxiqlktfvxsswxiksqlkinslpskkplxmkwkkplkklpll
hxhklvnslptftskkfq*evlnlltvsnh

Frame B:
hsftnqnkkcyqif*kstvkh*di*ellpqrvvklntilkkpihtyvmvhltqllptkmn
sfhsslkcqdledsklfvmvytkkn*levsviytlvkklfvqv*kvqllkmiillqhtei
tlicyqeeqhqkkflqny**KKLVVQRVKVVQCICLQRISMVVMVLLVLNVHFGAGIAFA
QKYNKTG---

---rflxswslccrlksrwygcfrrqrsw*irr*mv*sw*wsnhfxngylslcrslyv*s
ryhlsykrrs*pr*tnxrsn*khssnhlgq*nrn*rstrxhxrnrxr*ngksl*ksyrcs
ixtss*tlyqrlpprssskrc*ic*qfqti

Frame C:
ilsqtkiknvikffksqq*strtyknfclkew*n*tqf*ksryilm*wsi*lncyqqr*t
hfilh*nvki*ktrncl*wfiqkkin*rflsfihwsrsclcrfrkcny*r*syyysiqrs
hlyvikrsntrrnfcriinernwlfkg*rwfnayvykeflww*wycwcsmstlvlvlplx
kstirp---

---DFYXRGHYVAGLKVDGMDVFAVKEAGKYAAEWCRAGNGPIILXMDTYRYVGHSMXDP
GITYRTREEVNHVRQTXDPIENIRXIILXNKIATEDQLAXIXETVXDEMEKASEKATAAP
XPQARELFTNVYLQEVPVRGVEFVNSFKP*
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFB838 (VFB838Q) /CSM/VF/VFB8-B/VFB838Q.Seq.d/                       1834   0.0
AFG132 (AFG132Q) /CSM/AF/AFG1-B/AFG132Q.Seq.d/                       1039   0.0
SFI644 (SFI644Q) /CSM/SF/SFI6-B/SFI644Q.Seq.d/                       1035   0.0
VFG594 (VFG594Q) /CSM/VF/VFG5-D/VFG594Q.Seq.d/                       1019   0.0
VFL810 (VFL810Q) /CSM/VF/VFL8-A/VFL810Q.Seq.d/                       1015   0.0
VFK514 (VFK514Q) /CSM/VF/VFK5-A/VFK514Q.Seq.d/                       1015   0.0
SFD783 (SFD783Q) /CSM/SF/SFD7-D/SFD783Q.Seq.d/                       1015   0.0
CFH686 (CFH686Q) /CSM/CF/CFH6-D/CFH686Q.Seq.d/                        997   0.0
SSE806 (SSE806Q) /CSM/SS/SSE8-A/SSE806Q.Seq.d/                        961   0.0
SFE128 (SFE128Q) /CSM/SF/SFE1-B/SFE128Q.Seq.d/                        954   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AF209924|AF209924.1 Lycopersicon esculentum pyruvate dehydrogenase mRNA, partial cds.    42  1e-11  4
BM111840|BM111840.1 EST559376 potato roots Solanum tuberosum cDNA clone cPRO12F13 5' end, mRNA sequence.    42  2e-10  4
BI514833|BI514833.1 BB160016B10B04.5 Bee Brain Normalized Library, BB16 Apis mellifera cDNA clone BB160016B10B04 5', mRNA sequence.    56  3e-10  3
CB064381|CB064381.1 py07e03.y1 Haemonchus contortus whole worm pAMP1 v1 Haemonchus contortus cDNA 5' similar to SW:ODPA_ASCSU P26267 PYRUVATE DEHYDROGENASE E1 COMPONENT ALPHA SUBUNIT TYPE I, MITOCHONDRIAL PRECURSOR ;, mRNA sequence.    60  3e-10  3
CB191084|CB191084.1 py39h04.y1 Haemonchus contortus whole worm pAMP1 v1 Haemonchus contortus cDNA 5' similar to SW:ODPA_ASCSU P26267 PYRUVATE DEHYDROGENASE E1 COMPONENT ALPHA SUBUNIT TYPE I, MITOCHONDRIAL PRECURSOR ;, mRNA sequence.    60  3e-10  3
CA869913|CA869913.1 px13e02.y1 Haemonchus contortus intestine pAMP1 v1 Haemonchus contortus cDNA 5' similar to SW:ODPA_CAEEL P52899 PROBABLE PYRUVATE DEHYDROGENASE E1 COMPONENT ALPHA SUBUNIT, MITOCHONDRIAL PRECURSOR ;, mRNA sequence.    60  3e-10  3
CB063951|CB063951.1 py02b02.y1 Haemonchus contortus whole worm pAMP1 v1 Haemonchus contortus cDNA 5' similar to SW:ODPA_CAEEL P52899 PROBABLE PYRUVATE DEHYDROGENASE E1 COMPONENT ALPHA SUBUNIT, MITOCHONDRIAL PRECURSOR ;, mRNA sequence.    60  3e-10  3
CB099517|CB099517.1 py13e09.y1 Haemonchus contortus whole worm pAMP1 v1 Haemonchus contortus cDNA 5' similar to SW:ODPA_CAEEL P52899 PROBABLE PYRUVATE DEHYDROGENASE E1 COMPONENT ALPHA SUBUNIT, MITOCHONDRIAL PRECURSOR ;, mRNA sequence.    60  3e-10  3
CA869182|CA869182.1 pw99b06.y1 Haemonchus contortus intestine pAMP1 v1 Haemonchus contortus cDNA 5' similar to SW:ODPA_CAEEL P52899 PROBABLE PYRUVATE DEHYDROGENASE E1 COMPONENT ALPHA SUBUNIT, MITOCHONDRIAL PRECURSOR ;, mRNA sequence.    60  3e-10  3
CA868872|CA868872.1 pw91b05.y1 Haemonchus contortus intestine pAMP1 v1 Haemonchus contortus cDNA 5' similar to SW:ODPA_CAEEL P52899 PROBABLE PYRUVATE DEHYDROGENASE E1 COMPONENT ALPHA SUBUNIT, MITOCHONDRIAL PRECURSOR ;, mRNA sequence.    60  3e-10  3
dna update 2003. 9.10
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54C70) RecName: Full=Pyruvate dehydrogenase E1 component subun...   339   e-168
BC067306_1(BC067306|pid:none) Xenopus tropicalis pyruvate dehydr...   160   4e-72
(Q10489) RecName: Full=Pyruvate dehydrogenase E1 component subun...   153   3e-61
EU960716_1(EU960716|pid:none) Zea mays clone 227859 unknown mRNA.     162   3e-40
EU186336_1(EU186336|pid:none) Brassica rapa clone S66 pyruvate d...   167   7e-40
CR761539_1(CR761539|pid:none) Xenopus tropicalis finished cDNA, ...   166   2e-39
(Q8H1Y0) RecName: Full=Pyruvate dehydrogenase E1 component subun...   164   3e-39
BC080995_1(BC080995|pid:none) Xenopus laevis pyruvate dehydrogen...   163   1e-38
BC106670_1(BC106670|pid:none) Xenopus laevis pyruvate dehydrogen...   163   1e-38
BT036232_1(BT036232|pid:none) Zea mays full-length cDNA clone ZM...   162   2e-38
protein update 2009. 6.17
PSORT 

 psg: 0.83  gvh: 0.58  alm: 0.23  top: 0.50  tms: 0.07  mit: 0.38  mip: 0.09
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: endoplasmic reticulum
	 20.0 %: mitochondrial
	 16.0 %: cytoplasmic
	 12.0 %: Golgi
	 12.0 %: nuclear
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system
	  4.0 %: peroxisomal

>> prediction for VFB838 is end
5' end seq. ID VFB838F
5' end seq. 
>VFB838F.Seq
ACATTCTTTCACAAACCAAAATAAAAAATGTTATCAAATTTTTTAAAAGTCAACAGTAAA
GCACTAGGACATATAAGAACTTTTGCCTCAAAGAGTGGTGAAATTAAACACAATTTTAAA
AAAGCCGATACATACTTATGTGATGGTCCATCTGACTCAACTGTTACCAACAAAGATGAA
CTCATTTCATTCTTCACTGAAATGTCAAGATTTAGAAGACTCGAAACTGTTTGTGATGGT
TTATACAAAAAAAAATTAATTAGAGGTTTCTGTCATTTATACACTGGTCAAGAAGCTGTT
TGTGCAGGTTTAGAAAGTGCAATTACTAAAGATGATCATATTATTACAGCATACAGAGAT
CACACTTATATGTTATCAAGAGGAGCAACACCAGAAGAAATTTTTGCAGAATTATTAATG
AAAGAAACTGGTTGTTCAAAGGGTAAAGGTGGTTCAATGCATATGTTTACAAAGAATTTC
TATGGTGGTAATGGTATTGTTGGTGCTCAATGTCCACTTTGGTGCTGGTATTGCCTTTGC
NCAAAAGTACAATAAGACCGGT----------
Length of 5' end seq. 562
3' end seq. ID VFB838Z
3' end seq. 
>VFB838Z.Seq
----------ACGATTTCTATANTCGTGGTCACTATGTTGCAGGCTTAAAAGTCGATGGT
ATGGATGTTTTCGCCGTCAAAGAAGCTGGTAAATACGCCGCTGAATGGTGTAGAGCTGGT
AATGGTCCAATCATTTTANAAATGGATACCTATCGTTATGTAGGTCACTCTATGTNTGAT
CCAGGTATCACTTATCGTACAAGAGAAGAAGTTAACCACGTTAGACAAACTANAGATCCA
ATTGAAAACATTCGTCNAATCATCTTGGNCAATAAAATCGCAACTGAAGATCAACTCGCT
NCCATCNAAGAAACCGTTANAGATGAAATGGAAAAAGCCTCTGAAAAAGCTACCGCTGCT
CCATTNCCACAAGCTCGTGAACTCTTTACCAACGTTTACCTCCAAGAAGTTCCAGTAAGA
GGTGTTGAATTTGTTAACAGTTTCAAACCATAA
Length of 3' end seq. 443
Connected seq. ID VFB838P
Connected seq. 
>VFB838P.Seq
ACATTCTTTCACAAACCAAAATAAAAAATGTTATCAAATTTTTTAAAAGTCAACAGTAAA
GCACTAGGACATATAAGAACTTTTGCCTCAAAGAGTGGTGAAATTAAACACAATTTTAAA
AAAGCCGATACATACTTATGTGATGGTCCATCTGACTCAACTGTTACCAACAAAGATGAA
CTCATTTCATTCTTCACTGAAATGTCAAGATTTAGAAGACTCGAAACTGTTTGTGATGGT
TTATACAAAAAAAAATTAATTAGAGGTTTCTGTCATTTATACACTGGTCAAGAAGCTGTT
TGTGCAGGTTTAGAAAGTGCAATTACTAAAGATGATCATATTATTACAGCATACAGAGAT
CACACTTATATGTTATCAAGAGGAGCAACACCAGAAGAAATTTTTGCAGAATTATTAATG
AAAGAAACTGGTTGTTCAAAGGGTAAAGGTGGTTCAATGCATATGTTTACAAAGAATTTC
TATGGTGGTAATGGTATTGTTGGTGCTCAATGTCCACTTTGGTGCTGGTATTGCCTTTGC
NCAAAAGTACAATAAGACCGGT----------ACGATTTCTATANTCGTGGTCACTATGT
TGCAGGCTTAAAAGTCGATGGTATGGATGTTTTCGCCGTCAAAGAAGCTGGTAAATACGC
CGCTGAATGGTGTAGAGCTGGTAATGGTCCAATCATTTTANAAATGGATACCTATCGTTA
TGTAGGTCACTCTATGTNTGATCCAGGTATCACTTATCGTACAAGAGAAGAAGTTAACCA
CGTTAGACAAACTANAGATCCAATTGAAAACATTCGTCNAATCATCTTGGNCAATAAAAT
CGCAACTGAAGATCAACTCGCTNCCATCNAAGAAACCGTTANAGATGAAATGGAAAAAGC
CTCTGAAAAAGCTACCGCTGCTCCATTNCCACAAGCTCGTGAACTCTTTACCAACGTTTA
CCTCCAAGAAGTTCCAGTAAGAGGTGTTGAATTTGTTAACAGTTTCAAACCATAA
Length of connected seq. 1005
Full length Seq ID -
Full length Seq. -
Length of full length seq. -