>VFB830 (VFB830Q) /CSM/VF/VFB8-B/VFB830Q.Seq.d/
AAATATTAATATAATTAAAAATGTTAAGAATTAACAGAATTTCTAACTTACGTACTTTTG
GTCAACGTTTCTTCTCAACTGAACAACAAGATGTTGTTGTTATTGGTGGTGGACCAGGTG
GTTATGTTGCAGGTATTAAAGCAGGTCAATTAGGTATGAAAGTTACAGTTGTAGAGAAAA
GAGGTAAATTAGGAGGAACATGTTTAAATGTTGGTTGTATTCCATCAAAAGCATTATTGA
ATGCATCACATCTTTATGAAGAAGCCACCACTAAAATGTCCAAATATGGTGTCAAATGTT
CAGGTGTTGAATTAGATTTGGGAGCAATGATGCAATACAAAGATAAATCAGTCAGTGGCT
TAACATCAGGTATTGAAGGTTTATTCAAAAAGAACAAAGTAAAATATGATAAAGGTTTCG
GTAAAATCACTGGCCCAAACACTGTCGAAGTTACATTAAATGACGGTTCCGTTAAAACTA
TCGAAACCAAAAATATTGTCATTGCAACCGGTTCAGAGGTTACATCACTTCCAAATGTTA
ACATCGATGAAGAGTCTATCATCTCTXXXXXXXXXXGTAAGGTTACTGTAACCGTTGAAC
AAGTTGGTGCTGGTGGTTTCACTGGTACTCTCGAAGCTGATGCTGTCCTTGTCTCTGTTG
GTCGTCGTCCAAATACTAGTGGTCTTGGTCTTGAATCAGTTGGTATCCCAACCGATAAAG
CTGGTCGTGTCGAAGTTGGAGATCACTTTAACACTAAAGTTCCATCAATTTTCGCAATTG
GTGATGCCATCCGTGGTCCAATGCTTGCTCACAAAGCTGAAGAAGAAGGTATTGCTATCA
TTGAACAAATTCACAATGGTGGTGGTCATGTTAACTATGGTGCCATCCCATCCATTATCT
ACACTCACCCAGAAGTTGCTTGGGTTGGTAAGACTGAAGAAGAATTACAAAAAGAAGGTA
TCCAATACAATATTGGTAGATTCCCATTCGTTGCAAACTCTAGAGCCAAAACAAACGATG
ATGTCGAAGGTTTCGTTAAATTCCTCGCCGCTAAAGATTCTGATAGAGTCCTTGGTGCTC
ATATTATGGGTACCAATGCTGGTGAATTAATTGGTGAATGTGTTTTAGCTATGGAATATG
GTGCCTCATGTGAAGATATTGCAAGAACTTGTCATGGTCATCCAACTTTATCCGAAGCTG
TTAAAGAAGC

ili*LKMLRINRISNLRTFGQRFFSTEQQDVVVIGGGPGGYVAGIKAGQLGMKVTVVEKR
GKLGGTCLNVGCIPSKALLNASHLYEEATTKMSKYGVKCSGVELDLGAMMQYKDKSVSGL
TSGIEGLFKKNKVKYDKGFGKITGPNTVEVTLNDGSVKTIETKNIVIATGSEVTSLPNVN
IDEESIIS---

---KVTVTVEQVGAGGFTGTLEADAVLVSVGRRPNTSGLGLESVGIPTDKAGRVEVGDHF
NTKVPSIFAIGDAIRGPMLAHKAEEEGIAIIEQIHNGGGHVNYGAIPSIIYTHPEVAWVG
KTEEELQKEGIQYNIGRFPFVANSRAKTNDDVEGFVKFLAAKDSDRVLGAHIMGTNAGEL
IGECVLAMEYGASCEDIARTCHGHPTLSEAVKE

Frame A:
ky*yn*kc*eltefltyvllvnvssqlnnkmllllvvdqvvmlqvlkqvn*v*klql*rk
evn*eehv*mlvvfhqkhy*mhhifmkkpplkcpnmvsnvqvln*iweq*cntkinqsva
*hqvlkvyskrtk*nmikvsvkslaqtlsklh*mtvplklskpkilslqpvqrlhhfqml
tsmkslss---

---vrll*plnklvlvvslvlsklmlslsllvvvqilvvlvlnqlvsqpiklvvskleit
ltlkfhqfsqlvmpsvvqclltklkkkvllslnkftmvvvmltmvpshplstltqkllgl
vrlkknykkkvsntilvdshslqtlepkqtmmskvslnssplkilieslvlilwvpmlvn
*lvnvf*lwnmvphvkilqelvmviqlypkllkk

Frame B:
niniiknvkn*qnf*ltyfwstflln*ttrcccywwwtrwlccry*srsiryesyscrek
r*irrnmfkcwlysiksiiecitsl*rshh*nvqiwcqmfrc*irfgsndaiqr*isqwl
niry*rfiqkeqski**rfr*nhwpkhcrsyik*rfr*nyrnqkychcnrfrgyitskc*
hr*rvyhl---

---*gycnr*tswcwwfhwysrs*ccpclcwsssky*wsws*iswypnr*swscrswrsl
*h*ssinfrnw*chpwsnacsqs*rrrycyh*tnsqwwwsc*lwchpihylhsprsclgw
*d*rritkrrypiqyw*ipirckl*sqnkr*crrfr*iprr*rf**spwcsyygyqcw*i
nw*mcfsygiwclm*rycknlswssnfirsc*rs

Frame C:
ili*LKMLRINRISNLRTFGQRFFSTEQQDVVVIGGGPGGYVAGIKAGQLGMKVTVVEKR
GKLGGTCLNVGCIPSKALLNASHLYEEATTKMSKYGVKCSGVELDLGAMMQYKDKSVSGL
TSGIEGLFKKNKVKYDKGFGKITGPNTVEVTLNDGSVKTIETKNIVIATGSEVTSLPNVN
IDEESIIS---

---KVTVTVEQVGAGGFTGTLEADAVLVSVGRRPNTSGLGLESVGIPTDKAGRVEVGDHF
NTKVPSIFAIGDAIRGPMLAHKAEEEGIAIIEQIHNGGGHVNYGAIPSIIYTHPEVAWVG
KTEEELQKEGIQYNIGRFPFVANSRAKTNDDVEGFVKFLAAKDSDRVLGAHIMGTNAGEL
IGECVLAMEYGASCEDIARTCHGHPTLSEAVKE

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFB830 (VFB830Q) /CSM/VF/VFB8-B/VFB830Q.Seq.d/                       2339   0.0
VFH845 (VFH845Q) /CSM/VF/VFH8-B/VFH845Q.Seq.d/                       1257   0.0
VFD715 (VFD715Q) /CSM/VF/VFD7-A/VFD715Q.Seq.d/                       1257   0.0
VFC622 (VFC622Q) /CSM/VF/VFC6-A/VFC622Q.Seq.d/                       1257   0.0
VFB625 (VFB625Q) /CSM/VF/VFB6-B/VFB625Q.Seq.d/                       1257   0.0
SFH666 (SFH666Q) /CSM/SF/SFH6-C/SFH666Q.Seq.d/                       1257   0.0
AFF150 (AFF150Q) /CSM/AF/AFF1-C/AFF150Q.Seq.d/                       1257   0.0
AFE149 (AFE149Q) /CSM/AF/AFE1-C/AFE149Q.Seq.d/                       1257   0.0
AFD276 (AFD276Q) /CSM/AF/AFD2-D/AFD276Q.Seq.d/                       1257   0.0
AFC230 (AFC230Q) /CSM/AF/AFC2-B/AFC230Q.Seq.d/                       1257   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AX489387|AX489387.1 Sequence 6687 from Patent WO02053728.         56  3e-37  6
AR180935|AR180935.1 Sequence 7 from patent US 6333164.            56  2e-32  6
AL423544|AL423544.1 T3 end of clone AZ0AA009B01 of library AZ0AA from strain CBS 712 of Kluyveromyces marxianus.    84  2e-23  4
BZ296408|BZ296408.1 CG2037.f11 Candida glabrata Random Genomic Library Candida glabrata genomic clone CG2037, DNA sequence.    64  7e-23  4
AL428740|AL428740.1 clone BA0AB027E08 of library BA0AB from strain CLIB 210 of  Kluyveromyces lactis.    70  2e-20  3
AL439032|AL439032.1 T3 end of clone BD0AA002A06 of library BD0AA from strain CBS 94 of  Candida tropicalis.    54  3e-19  5
J03489|J03489.1 Pig lipoamide dehydrogenase mRNA, complete cds.    42  4e-15  5
J03620|J03620.1 Human dihydrolipoamide dehydrogenase mRNA, complete cds.    46  4e-15  4
BC018696|BC018696.2 Homo sapiens dihydrolipoamide dehydrogenase (E3 component of pyruvate dehydrogenase complex, 2-oxo-glutarate complex, branched chain keto acid dehydrogenase complex), mRNA (cDNA clone MGC:3866 IMAGE:3029752), complete cds.    46  4e-15  4
BC018648|BC018648.2 Homo sapiens dihydrolipoamide dehydrogenase (E3 component of pyruvate dehydrogenase complex, 2-oxo-glutarate complex, branched chain keto acid dehydrogenase complex), mRNA (cDNA clone MGC:2115 IMAGE:3029752), complete cds.    46  4e-15  4

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CR954211_302(CR954211|pid:none) Ostreococcus tauri strain OTTH05...   363   7e-99
AF542182_1(AF542182|pid:none) Lycopersicon esculentum dihydrolip...   359   9e-98
AF295339_1(AF295339|pid:none) Solanum tuberosum dihydrolipoamide...   358   2e-97
EU616551_1(EU616551|pid:none) Capsicum annuum putative branched-...   355   2e-96
(Q9M5K2) RecName: Full=Dihydrolipoyl dehydrogenase 2, mitochondr...   350   4e-95
AJ223804_1(AJ223804|pid:none) Arabidopsis thaliana mRNA for 2-ox...   350   4e-95
Z82277_3(Z82277|pid:none) Caenorhabditis elegans Cosmid LLC1, co...   348   3e-94
AP002869_2(AP002869|pid:none) Oryza sativa Japonica Group genomi...   346   1e-93
AF074940_1(AF074940|pid:none) Glycine max ferric leghemoglobin r...   345   2e-93
AC023673_28(AC023673|pid:none) Genomic sequence for Arabidopsis ...   345   2e-93

 psg: 0.71  gvh: 0.36  alm: 0.42  top: 0.53  tms: 0.00  mit: 0.46  mip: 0.04
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: mitochondrial
	 28.0 %: cytoplasmic
	 16.0 %: nuclear

>> prediction for VFB830 is mit

>VFB830F.Seq
AAATATTAATATAATTAAAAATGTTAAGAATTAACAGAATTTCTAACTTACGTACTTTTG
GTCAACGTTTCTTCTCAACTGAACAACAAGATGTTGTTGTTATTGGTGGTGGACCAGGTG
GTTATGTTGCAGGTATTAAAGCAGGTCAATTAGGTATGAAAGTTACAGTTGTAGAGAAAA
GAGGTAAATTAGGAGGAACATGTTTAAATGTTGGTTGTATTCCATCAAAAGCATTATTGA
ATGCATCACATCTTTATGAAGAAGCCACCACTAAAATGTCCAAATATGGTGTCAAATGTT
CAGGTGTTGAATTAGATTTGGGAGCAATGATGCAATACAAAGATAAATCAGTCAGTGGCT
TAACATCAGGTATTGAAGGTTTATTCAAAAAGAACAAAGTAAAATATGATAAAGGTTTCG
GTAAAATCACTGGCCCAAACACTGTCGAAGTTACATTAAATGACGGTTCCGTTAAAACTA
TCGAAACCAAAAATATTGTCATTGCAACCGGTTCAGAGGTTACATCACTTCCAAATGTTA
ACATCGATGAAGAGTCTATCATCTCT----------

>VFB830Z.Seq
----------GTAAGGTTACTGTAACCGTTGAACAAGTTGGTGCTGGTGGTTTCACTGGT
ACTCTCGAAGCTGATGCTGTCCTTGTCTCTGTTGGTCGTCGTCCAAATACTAGTGGTCTT
GGTCTTGAATCAGTTGGTATCCCAACCGATAAAGCTGGTCGTGTCGAAGTTGGAGATCAC
TTTAACACTAAAGTTCCATCAATTTTCGCAATTGGTGATGCCATCCGTGGTCCAATGCTT
GCTCACAAAGCTGAAGAAGAAGGTATTGCTATCATTGAACAAATTCACAATGGTGGTGGT
CATGTTAACTATGGTGCCATCCCATCCATTATCTACACTCACCCAGAAGTTGCTTGGGTT
GGTAAGACTGAAGAAGAATTACAAAAAGAAGGTATCCAATACAATATTGGTAGATTCCCA
TTCGTTGCAAACTCTAGAGCCAAAACAAACGATGATGTCGAAGGTTTCGTTAAATTCCTC
GCCGCTAAAGATTCTGATAGAGTCCTTGGTGCTCATATTATGGGTACCAATGCTGGTGAA
TTAATTGGTGAATGTGTTTTAGCTATGGAATATGGTGCCTCATGTGAAGATATTGCAAGA
ACTTGTCATGGTCATCCAACTTTATCCGAAGCTGTTAAAGAAGC

>VFB830P.Seq
AAATATTAATATAATTAAAAATGTTAAGAATTAACAGAATTTCTAACTTACGTACTTTTG
GTCAACGTTTCTTCTCAACTGAACAACAAGATGTTGTTGTTATTGGTGGTGGACCAGGTG
GTTATGTTGCAGGTATTAAAGCAGGTCAATTAGGTATGAAAGTTACAGTTGTAGAGAAAA
GAGGTAAATTAGGAGGAACATGTTTAAATGTTGGTTGTATTCCATCAAAAGCATTATTGA
ATGCATCACATCTTTATGAAGAAGCCACCACTAAAATGTCCAAATATGGTGTCAAATGTT
CAGGTGTTGAATTAGATTTGGGAGCAATGATGCAATACAAAGATAAATCAGTCAGTGGCT
TAACATCAGGTATTGAAGGTTTATTCAAAAAGAACAAAGTAAAATATGATAAAGGTTTCG
GTAAAATCACTGGCCCAAACACTGTCGAAGTTACATTAAATGACGGTTCCGTTAAAACTA
TCGAAACCAAAAATATTGTCATTGCAACCGGTTCAGAGGTTACATCACTTCCAAATGTTA
ACATCGATGAAGAGTCTATCATCTCT----------GTAAGGTTACTGTAACCGTTGAAC
AAGTTGGTGCTGGTGGTTTCACTGGTACTCTCGAAGCTGATGCTGTCCTTGTCTCTGTTG
GTCGTCGTCCAAATACTAGTGGTCTTGGTCTTGAATCAGTTGGTATCCCAACCGATAAAG
CTGGTCGTGTCGAAGTTGGAGATCACTTTAACACTAAAGTTCCATCAATTTTCGCAATTG
GTGATGCCATCCGTGGTCCAATGCTTGCTCACAAAGCTGAAGAAGAAGGTATTGCTATCA
TTGAACAAATTCACAATGGTGGTGGTCATGTTAACTATGGTGCCATCCCATCCATTATCT
ACACTCACCCAGAAGTTGCTTGGGTTGGTAAGACTGAAGAAGAATTACAAAAAGAAGGTA
TCCAATACAATATTGGTAGATTCCCATTCGTTGCAAACTCTAGAGCCAAAACAAACGATG
ATGTCGAAGGTTTCGTTAAATTCCTCGCCGCTAAAGATTCTGATAGAGTCCTTGGTGCTC
ATATTATGGGTACCAATGCTGGTGAATTAATTGGTGAATGTGTTTTAGCTATGGAATATG
GTGCCTCATGTGAAGATATTGCAAGAACTTGTCATGGTCATCCAACTTTATCCGAAGCTG
TTAAAGAAGC