>VFB829 (VFB829Q) /CSM/VF/VFB8-B/VFB829Q.Seq.d/
XXXXXXXXXXCACACTCAAATATCGTACTCAAGATTGGGAAGCTTTTGTAAAGGATGGTA
AAAATGGTGCAATTCGTGTTAGTGTCGCCACAAATTTCGATAAGGCCGCTTACGTCATTG
GTAAATTGTCTTTTGTTTTCTTCCGTTTCATCCTTCCACTCCGTTATCATAGCTTTACAG
ATTTAATTTGTTATTTCCTCATTGCTGAATTCGTCTTTGGTTGGTATCTCACAATTAATT
TCCAAGTTAGTCATGTCGCTGAAGATCTCAAATTCTTTGCTACCCCTGAAAGACCAGATG
AACCATCTCAAATCAATGAAGATTGGGCAATCCTTCAACTTAAAACTACTCAAGATTATG
GTCATGGTTCACTCCTTTGTACCTTTTTTAGTGGTTCTTTAAATCATCAAGTTGTTCATC
ATTTATTCCCATCAATTGCTCAAGATTTCTACCCACAACTTGTACCAATTGTAAAAGAAG
TTTGTAAAGAACATAACATTACTTACCACATTAAACCAAACTTCACTGAAGCTATTATGT
CACACATTAATTACCTTTACAAAATGGGTAATGATCCAGATTATGTTAAAAAACCATTAG
CCTCAAAAGA

---TLKYRTQDWEAFVKDGKNGAIRVSVATNFDKAAYVIGKLSFVFFRFILPLRYHSFTD
LICYFLIAEFVFGWYLTINFQVSHVAEDLKFFATPERPDEPSQINEDWAILQLKTTQDYG
HGSLLCTFFSGSLNHQVVHHLFPSIAQDFYPQLVPIVKEVCKEHNITYHIKPNFTEAIMS
HINYLYKMGNDPDYVKKPLASK

Frame A:
---TLKYRTQDWEAFVKDGKNGAIRVSVATNFDKAAYVIGKLSFVFFRFILPLRYHSFTD
LICYFLIAEFVFGWYLTINFQVSHVAEDLKFFATPERPDEPSQINEDWAILQLKTTQDYG
HGSLLCTFFSGSLNHQVVHHLFPSIAQDFYPQLVPIVKEVCKEHNITYHIKPNFTEAIMS
HINYLYKMGNDPDYVKKPLASK


Frame B:
---hsnivlkigkll*rmvkmvqfvlvspqisirpltslvncllfssvssfhsviialqi
*fvissllnsslvgisqlisklvmslkisnsllplkdqmnhlksmkigqsfnlkllkimv
mvhsfvpflvvl*iiklfiiyshqllkisthnlyql*kkfvknitllttlnqtslkllch
tlitftkwvmiqimlknh*pqk


Frame C:
---tqisysrlgsfckgw*kwcnsc*crhkfr*grlrhw*ivfcflpfhpstpls*lyrf
nllfphc*irlwlvshn*fps*scr*rsqilcyp*ktr*tisnq*rlgnpst*nysrlws
wftplylf*wffkssscssfipincsrflpttctnckrsl*rt*hylph*tklh*syyvt
h*lplqng**srlc*ktislkr

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFN123 (VFN123Q) /CSM/VF/VFN1-A/VFN123Q.Seq.d/                       1189   0.0
VFM295 (VFM295Q) /CSM/VF/VFM2-D/VFM295Q.Seq.d/                       1189   0.0
VFI562 (VFI562Q) /CSM/VF/VFI5-C/VFI562Q.Seq.d/                       1189   0.0
VFE188 (VFE188Q) /CSM/VF/VFE1-D/VFE188Q.Seq.d/                       1189   0.0
VFB829 (VFB829Q) /CSM/VF/VFB8-B/VFB829Q.Seq.d/                       1189   0.0
SSB813 (SSB813Q) /CSM/SS/SSB8-A/SSB813Q.Seq.d/                       1189   0.0
SLA259 (SLA259Q) /CSM/SL/SLA2-C/SLA259Q.Seq.d/                       1189   0.0
SLA256 (SLA256Q) /CSM/SL/SLA2-C/SLA256Q.Seq.d/                       1189   0.0
CFC256 (CFC256Q) /CSM/CF/CFC2-C/CFC256Q.Seq.d/                       1189   0.0
VFN880 (VFN880Q) /CSM/VF/VFN8-D/VFN880Q.Seq.d/                       1181   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

BD092935|BD092935.1 Methods and compositions for synthesis of long chain polyunsaturated fatty acids in plants.  1189  0.0  1
BD082645|BD082645.1 Methods and compositions for synthesis of long chain polyunsaturated fatty acids.  1189  0.0  1
BD082630|BD082630.1 Methods and compositions for synthesis of long chain poly-unsaturated fatty acids.  1189  0.0  1
AB029311|AB029311.1 Dictyostelium discoideum Dd des5 gene for fatty acid desaturase, complete cds.   823  0.0  2
AB022097|AB022097.1 Dictyostelium discoideum mRNA for delta 5 fatty acid desaturase, complete cds.    90  2e-47  6
BF793106|BF793106.1 602254090F1 NIH_MGC_84 Homo sapiens cDNA clone IMAGE:4346086 5', mRNA sequence.    36  0.66  2
BM269513|BM269513.1 MEST410-C03.univ ISUM5-RN Zea mays cDNA clone MEST410-C03 3', mRNA sequence.    44  2.0  1
BU261781|BU261781.1 603503358F1 CSEQCHN51 Gallus gallus cDNA clone ChEST424c15 5', mRNA sequence.    44  2.0  1
AL591476|AL591476.7 Zebrafish DNA sequence from clone XX-263J20 on chromosome 7 Contains a gene for a novel kinase with a CNH domain, one complete and part  of five genes for novel proteins similar to novel immune-type  receptors (nitr), a novel gene similar to nitr4 and part of a novel  gene similar to nitr1.    36  2.4  4
AL591427|AL591427.2 Zebrafish DNA sequence from clone XX-219H16 on chromosome 7 Contains two novel genes for proteins similar to novel immune-type receptor 1 (nitr1), part of nine novel nitr genes, part of a novel gene similar to nitr2, the nitr1.1 gene, the nitr1.3 gene, a putative novel gene  and a novel gene similar to nitr4.    36  2.5  5

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q9Y1W0) RecName: Full=Delta(5) fatty acid desaturase A;        ...   417   e-115
(O96099) RecName: Full=Delta(5) fatty acid desaturase B;        ...   271   8e-72
(Q1ZXQ5) RecName: Full=Probable Delta(5) fatty acid desaturase C...   225   7e-58
AF419297_1(AF419297|pid:none) Pythium irregulare delta-5 fatty a...   149   6e-35
AB188307_1(AB188307|pid:none) Mortierella alpina gene for delta5...   144   2e-33
(O74212) RecName: Full=Delta(5) fatty acid desaturase;          ...   143   4e-33
CP000592_278(CP000592|pid:none) Ostreococcus lucimarinus CCE9901...   138   1e-31
EF678404_1(EF678404|pid:none) Picea sitchensis clone WS0291_A04 ...   127   2e-28
CP001574_328(CP001574|pid:none) Micromonas sp. RCC299 chromosome...   125   1e-27
CS020137_1(CS020137|pid:none) Sequence 91 from Patent WO20050123...   121   1e-26

 psg: 0.52  gvh: 0.43  alm: 0.33  top: 0.53  tms: 0.00  mit: 0.27  mip: 0.02
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 64.0 %: cytoplasmic
	 20.0 %: nuclear
	  8.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: peroxisomal

>> prediction for VFB829 is cyt

>VFB829Z.Seq
----------CACACTCAAATATCGTACTCAAGATTGGGAAGCTTTTGTAAAGGATGGTA
AAAATGGTGCAATTCGTGTTAGTGTCGCCACAAATTTCGATAAGGCCGCTTACGTCATTG
GTAAATTGTCTTTTGTTTTCTTCCGTTTCATCCTTCCACTCCGTTATCATAGCTTTACAG
ATTTAATTTGTTATTTCCTCATTGCTGAATTCGTCTTTGGTTGGTATCTCACAATTAATT
TCCAAGTTAGTCATGTCGCTGAAGATCTCAAATTCTTTGCTACCCCTGAAAGACCAGATG
AACCATCTCAAATCAATGAAGATTGGGCAATCCTTCAACTTAAAACTACTCAAGATTATG
GTCATGGTTCACTCCTTTGTACCTTTTTTAGTGGTTCTTTAAATCATCAAGTTGTTCATC
ATTTATTCCCATCAATTGCTCAAGATTTCTACCCACAACTTGTACCAATTGTAAAAGAAG
TTTGTAAAGAACATAACATTACTTACCACATTAAACCAAACTTCACTGAAGCTATTATGT
CACACATTAATTACCTTTACAAAATGGGTAATGATCCAGATTATGTTAAAAAACCATTAG
CCTCAAAAGA