>VFB826 (VFB826Q) /CSM/VF/VFB8-B/VFB826Q.Seq.d/
TATTAAAAAAAATTCTAGTAAGGTATAAAAAAAAAAATCATNTAAAANGGTAGAAAAAAT
CATTGTTTCAAATCCAGTTGCTAATCTTTTAGGTGATGAACAAACCAGAGTTATTTGGGA
TTTAATTGAAAAGAAATTAATTTTTCCATTTTTAGATTTAAAAGTTGAAACCTATGATTT
AGGAATTGAGTATCGNGATAAAACCAATGATCAAGTTACAATTGATGCAGCCAATGCAAT
TAAGAGATTAAAAGTTGGTATTAAATGTGCAACAATTACACCAGACGAAGCACGTGTCAC
AGAGTTTGGATTGAAGGAGATGTGGAAATCACCAAATGGTNCCATTCGTAATACATTGGG
NGGCNCATTATTTAGAGAGCCAATTGTTTGTAAGAATGTCCCACGTTTGGTCACCTGTNG
GAATAAATCTXXXXXXXXXX

ikknsskv*KKKSXKXVEKIIVSNPVANLLGDEQTRVIWDLIEKKLIFPFLDLKVETYDL
GIEYRDKTNDQVTIDAANAIKRLKVGIKCATITPDEARVTEFGLKEMWKSPNGXIRNTLG
GXLFREPIVCKNVPRLVTCXNK---

Frame A:
y*kkf**gikkkix*xgrknhcfkssc*sfr**tnqsylgfn*keinfsifrfks*nl*f
rn*vsx*nq*ssyn*csqcn*eikswy*mcnnytrrstchrvwiegdveitkwxhs*yig
xxii*rancl*ecptfghlxe*i---


Frame B:
ikknsskv*KKKSXKXVEKIIVSNPVANLLGDEQTRVIWDLIEKKLIFPFLDLKVETYDL
GIEYRDKTNDQVTIDAANAIKRLKVGIKCATITPDEARVTEFGLKEMWKSPNGXIRNTLG
GXLFREPIVCKNVPRLVTCXNK---


Frame C:
lkkilvrykkknhxkx*kkslfqiqllif*vmnkpelfgi*lkrn*ffhf*i*klkpmi*
elsixikpmiklqlmqpmqlrd*klvlnvqqlhqtkhvsqsld*rrcgnhqmvpfvihwx
ahylesqlfvrmshvwspvgin---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFF796 (VFF796Q) /CSM/VF/VFF7-D/VFF796Q.Seq.d/                        745   0.0
VFB826 (VFB826Q) /CSM/VF/VFB8-B/VFB826Q.Seq.d/                        745   0.0
VSJ261 (VSJ261Q) /CSM/VS/VSJ2-C/VSJ261Q.Seq.d/                        737   0.0
VFM475 (VFM475Q) /CSM/VF/VFM4-D/VFM475Q.Seq.d/                        737   0.0
VFM444 (VFM444Q) /CSM/VF/VFM4-B/VFM444Q.Seq.d/                        737   0.0
VFM392 (VFM392Q) /CSM/VF/VFM3-D/VFM392Q.Seq.d/                        737   0.0
VFL349 (VFL349Q) /CSM/VF/VFL3-C/VFL349Q.Seq.d/                        737   0.0
VFL180 (VFL180Q) /CSM/VF/VFL1-D/VFL180Q.Seq.d/                        737   0.0
VFG531 (VFG531Q) /CSM/VF/VFG5-B/VFG531Q.Seq.d/                        737   0.0
VFF886 (VFF886Q) /CSM/VF/VFF8-D/VFF886Q.Seq.d/                        737   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC115577|AC115577.2 Dictyostelium discoideum chromosome 2 map 4657875-4914984 strain AX4, complete sequence.   613  0.0  7
CD432704|CD432704.1 ETH1_25_H02.g1_A002 Ethylene-treated seedlings Sorghum bicolor cDNA clone ETH1_25_H02_A002 5', mRNA sequence.    56  4e-18  4
BQ579956|BQ579956.1 ACB18-jm9.c09 onion bulb meristem library Allium cepa cDNA, mRNA sequence.    50  2e-08  2
AJ437269|AJ437269.1 Cucumis sativus mRNA for isocitrate dehydrogenase.    56  3e-06  2
CB931031|CB931031.1 ri67e04.y1 Meloidogyne chitwoodi J2 pDNR LIB Meloidogyne chitwoodi cDNA 5' similar to TR:O17643 O17643 C34F6.8 PROTEIN. [1] ;, mRNA sequence.    46  2e-05  2
AB019327|AB019327.1 Daucus carota mRNA for NADP specific isocitrate dehydrogenase, complete cds.    58  1e-04  1
BP190561|BP190561.1 Dugesia japonica cDNA, clone: 04916_HH, expressed in the head.    44  2e-04  2
CD474757|CD474757.1 nad03-15ms2-d09 Nad03 Nuphar advena cDNA clone nad03-15ms2-d09 5', mRNA sequence.    42  6e-04  3
BG607427|BG607427.1 WHE2479_E11_I21ZS Triticum monococcum early reproductive apex cDNA library Triticum monococcum cDNA clone WHE2479_E11_I21, mRNA sequence.    40  8e-04  2
BQ482885|BQ482885.1 WHE0573_F04_L07ZE Triticum monococcum vegetative apex cDNA library Triticum monococcum cDNA clone WHE0573_F04_L07, mRNA sequence.    40  0.001  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q75JR3) RecName: Full=Isocitrate dehydrogenase [NADP] cytoplasm...   239   2e-62
DQ886272_1(DQ886272|pid:none) Gryllus firmus isocitrate dehydrog...   184   6e-46
CP000494_5093(CP000494|pid:none) Bradyrhizobium sp. BTAi1, compl...   183   2e-45
BT080533_1(BT080533|pid:none) Caligus clemensi clone ccle-evs-50...   182   3e-45
BT077406_1(BT077406|pid:none) Lepeophtheirus salmonis clone lsal...   181   7e-45
CP001074_2374(CP001074|pid:none) Rhizobium etli CIAT 652, comple...   181   9e-45
AM236080_2632(AM236080|pid:none) Rhizobium leguminosarum bv. vic...   180   1e-44
CP000490_129(CP000490|pid:none) Paracoccus denitrificans PD1222 ...   180   1e-44
CP000661_2344(CP000661|pid:none) Rhodobacter sphaeroides ATCC 17...   180   2e-44
BX572605_126(BX572605|pid:none) Rhodopseudomonas palustris CGA00...   180   2e-44

 psg: 0.84  gvh: 0.35  alm: 0.44  top: 0.13  tms: 0.00  mit: 0.24  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 40.0 %: nuclear
	  8.0 %: vacuolar
	  4.0 %: mitochondrial
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for VFB826 is cyt

>VFB826F.Seq
TATTAAAAAAAATTCTAGTAAGGTATAAAAAAAAAAATCATNTAAAANGGTAGAAAAAAT
CATTGTTTCAAATCCAGTTGCTAATCTTTTAGGTGATGAACAAACCAGAGTTATTTGGGA
TTTAATTGAAAAGAAATTAATTTTTCCATTTTTAGATTTAAAAGTTGAAACCTATGATTT
AGGAATTGAGTATCGNGATAAAACCAATGATCAAGTTACAATTGATGCAGCCAATGCAAT
TAAGAGATTAAAAGTTGGTATTAAATGTGCAACAATTACACCAGACGAAGCACGTGTCAC
AGAGTTTGGATTGAAGGAGATGTGGAAATCACCAAATGGTNCCATTCGTAATACATTGGG
NGGCNCATTATTTAGAGAGCCAATTGTTTGTAAGAATGTCCCACGTTTGGTCACCTGTNG
GAATAAATCT----------