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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFB804
Library VF
(Link to library)
Clone ID VFB804
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U13849-1
Original site URL
Representative seq. ID VFB804P
(Link to Original site)
Representative DNA sequence 
>VFB804 (VFB804Q) /CSM/VF/VFB8-A/VFB804Q.Seq.d/
TTTAATNTTGTNCCAAATCCAAGNGTTGGNTTAACAGAGGATCAAAANCAATTTCAATCA
ANGGCNTTAGATTTTGCNCAAGAAAAGATGAANCCNTTTGCAGAGAAANGGGATAAAGAG
GAATNTTTTCCAAGAGATGTGATGAGAGAAXXXXXXXXXXATGGTCAGAACGGGCGCGGA
GAAGGGACCAAAGGGGATCTCCTGTCTCTTGGTGGAGAAGGACACCCCAGGCCTTAGCTT
CGGCAAAAAGGAGGAGAAACTAGGCTGGAACACCCAACCAACTCGTGCATTGATTTTCGA
AGATTGTCGTGTACCAGTTGGCAATTTAATTGGCAAAGAGGGTCAAGGCTTTTCCATTGC
TATGAACGCTTTAAACGGCGGTCGTATCAACATTGGTGCTTGTAGTTTGGGTGGGGCCCA
ATCTTGTTTGGTGGCCGCTAGAGATCATGTTAAAGTTAGAAAACAATTCAATCAACCATT
GGAACATTTCCAAGCCGTTCAATTTAAAATGGCAGATATGGCCACTAAATTACATGCATC
TCGTATTATGATTAGAAATGCCGCTGCAATGTTGGATGCAAAAGATCCATCTGCTCATGT
TTACATTGCAATGGCAAAATTATTTGCTTGTGATGAATGTTTCAAAGTTACCGATGATTC
ACTTCAATTATTTGGTGGTTATGGTTATCTTAAAGATTATCCAGTTGAAAGATATTTACG
TGATCTTCGTGTTCATCGTATTTTGGAAGGTTCTGATGCTGTTATGAGATAATCATTTCA
AGAGAATTAAGTAAAGA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
FNXVPNPXVGLTEDQXQFQSXALDFAQEKMXPFAEKXDKEEXFPRDVMRE---

---MVRTGAEKGPKGISCLLVEKDTPGLSFGKKEEKLGWNTQPTRALIFEDCRVPVGNLI
GKEGQGFSIAMNALNGGRINIGACSLGGAQSCLVAARDHVKVRKQFNQPLEHFQAVQFKM
ADMATKLHASRIMIRNAAAMLDAKDPSAHVYIAMAKLFACDECFKVTDDSLQLFGGYGYL
KDYPVERYLRDLRVHRILEGSDAVMR*sfqen*vk
Translated Amino Acid sequence (All Frames) 
Frame A:
FNXVPNPXVGLTEDQXQFQSXALDFAQEKMXPFAEKXDKEEXFPRDVMRE---

---MVRTGAEKGPKGISCLLVEKDTPGLSFGKKEEKLGWNTQPTRALIFEDCRVPVGNLI
GKEGQGFSIAMNALNGGRINIGACSLGGAQSCLVAARDHVKVRKQFNQPLEHFQAVQFKM
ADMATKLHASRIMIRNAAAMLDAKDPSAHVYIAMAKLFACDECFKVTDDSLQLFGGYGYL
KDYPVERYLRDLRVHRILEGSDAVMR*sfqen*vk

Frame B:
lxlxqiqxlx*qrikxnfnqxx*ilxkkr*xxlqrxgikrnxfqem**e---

---wserarrrdqrgspvswwrrtpqalasakrrrn*agtpnqlvh*fskivvyqlai*l
akrvkafpll*tl*tavvstlvlvvwvgpnlvwwpleimlklennsinhwniskpfnlkw
qiwplnymhlvl*lemplqcwmqkihllmftlqwqnyllvmnvsklpmihfnylvvmvil
kiiqlkdiyvifvfivfwkvlmll*dnhfkrik*r

Frame C:
*xcxkskxwxnrgskxisixgxrfcxrkdexxcrexg*rgxfskrcder---

---gqngrgegtkgdllslggeghprp*lrqkggetrlehptnscidfrrlsctswqfnw
qrgsrlfhcyerfkrrsyqhwcl*fgwgpilfggr*rsc*s*ktiqstigtfpsrsi*ng
rygh*itcisyyd*kcrcnvgckrsicsclhcngkiicl**mfqsyr*ftsiiwwlwls*
rlss*kift*sscssyfgrf*ccyeiiisrelsk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFB804 (VFB804Q) /CSM/VF/VFB8-A/VFB804Q.Seq.d/                       1449   0.0
VFM512 (VFM512Q) /CSM/VF/VFM5-A/VFM512Q.Seq.d/                       1263   0.0
SFG829 (SFG829Q) /CSM/SF/SFG8-B/SFG829Q.Seq.d/                       1263   0.0
VFI287 (VFI287Q) /CSM/VF/VFI2-D/VFI287Q.Seq.d/                       1257   0.0
SFB688 (SFB688Q) /CSM/SF/SFB6-D/SFB688Q.Seq.d/                       1257   0.0
SFF648 (SFF648Q) /CSM/SF/SFF6-B/SFF648Q.Seq.d/                       1247   0.0
AFM805 (AFM805Q) /CSM/AF/AFM8-A/AFM805Q.Seq.d/                       1247   0.0
VFC645 (VFC645Q) /CSM/VF/VFC6-B/VFC645Q.Seq.d/                       1239   0.0
VFG172 (VFG172Q) /CSM/VF/VFG1-C/VFG172Q.Seq.d/                       1154   0.0
CFA163 (CFA163Q) /CSM/CF/CFA1-C/CFA163Q.Seq.d/                       1148   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

U17110|U17110.1 Clostridium acetobutylicum crotonase (crt), putative butyryl-CoA dehydrogenase (BCD), putative a-subunit of electron-transfer flavoprotein (etfA), putative b-subunit of electron-transfer flavoprotein (etfB), and 3-hydroxybutyryl-CoA dehydrogenase (hbd) genes, complete cds.    48  0.001  3
AE007768|AE007768.1 Clostridium acetobutylicum ATCC824 section 256 of 356 of the complete genome.    48  0.007  3
AL331969|AL331969.1 Tetraodon nigroviridis genome survey sequence T3 end of clone 004M03 of library A from Tetraodon nigroviridis.    50  0.043  1
CA347210|CA347210.1 678289 NCCCWA 1RT Oncorhynchus mykiss cDNA clone 1RT104I03_A_E02 5', mRNA sequence.    48  0.17  1
AC103202|AC103202.5 Rattus norvegicus clone CH230-35E14, *** SEQUENCING IN PROGRESS ***, 1 ordered piece.    40  0.55  4
AC024293|AC024293.18 Homo sapiens chromosome 11, clone RP11-425L10, complete sequence.    46  0.68  1
AC113158|AC113158.2 Mus musculus chromosome UNK clone RP23-101D11, WORKING DRAFT SEQUENCE, 63 unordered pieces.    44  2.7  1
CB075142|CB075142.1 hx15g08.b1 Gingko megasporophyll (NYBG) Gingko biloba cDNA clone hx15g08, mRNA sequence.    44  2.7  1
AC111770|AC111770.4 Rattus norvegicus clone CH230-170J3, *** SEQUENCING IN PROGRESS ***, 2 unordered pieces.    40  2.7  2
AC092625|AC092625.2 Homo sapiens BAC clone RP11-266L10 from 2, complete sequence.    34  6.9  4
dna update 2003. 9. 9
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54IM8) RecName: Full=Isobutyryl-CoA dehydrogenase, mitochondri...   412   e-114
AP007255_3586(AP007255|pid:none) Magnetospirillum magneticum AMB...   271   2e-71
CU459003_1469(CU459003|pid:none) Magnetospirillum gryphiswaldens...   270   5e-71
CP000613_1308(CP000613|pid:none) Rhodospirillum centenum SW, com...   270   5e-71
BC044203_1(BC044203|pid:none) Danio rerio acyl-Coenzyme A dehydr...   265   1e-69
(Q9UKU7) RecName: Full=Isobutyryl-CoA dehydrogenase, mitochondri...   263   4e-69
AK074640_1(AK074640|pid:none) Homo sapiens cDNA FLJ90159 fis, cl...   263   4e-69
BC001964_1(BC001964|pid:none) Homo sapiens acyl-Coenzyme A dehyd...   263   5e-69
BC056845_1(BC056845|pid:none) Xenopus laevis hypothetical protei...   262   8e-69
AK019502_1(AK019502|pid:none) Mus musculus 0 day neonate skin cD...   262   1e-68
protein update 2009. 6.17
PSORT 

 psg: 0.79  gvh: 0.29  alm: 0.41  top: 0.53  tms: 0.00  mit: 0.18  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 40.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system

>> prediction for VFB804 is cyt
5' end seq. ID VFB804F
5' end seq. 
>VFB804F.Seq
TTTAATNTTGTNCCAAATCCAAGNGTTGGNTTAACAGAGGATCAAAANCAATTTCAATCA
ANGGCNTTAGATTTTGCNCAAGAAAAGATGAANCCNTTTGCAGAGAAANGGGATAAAGAG
GAATNTTTTCCAAGAGATGTGATGAGAGAA----------
Length of 5' end seq. 150
3' end seq. ID VFB804Z
3' end seq. 
>VFB804Z.Seq
----------ATGGTCAGAACGGGCGCGGAGAAGGGACCAAAGGGGATCTCCTGTCTCTT
GGTGGAGAAGGACACCCCAGGCCTTAGCTTCGGCAAAAAGGAGGAGAAACTAGGCTGGAA
CACCCAACCAACTCGTGCATTGATTTTCGAAGATTGTCGTGTACCAGTTGGCAATTTAAT
TGGCAAAGAGGGTCAAGGCTTTTCCATTGCTATGAACGCTTTAAACGGCGGTCGTATCAA
CATTGGTGCTTGTAGTTTGGGTGGGGCCCAATCTTGTTTGGTGGCCGCTAGAGATCATGT
TAAAGTTAGAAAACAATTCAATCAACCATTGGAACATTTCCAAGCCGTTCAATTTAAAAT
GGCAGATATGGCCACTAAATTACATGCATCTCGTATTATGATTAGAAATGCCGCTGCAAT
GTTGGATGCAAAAGATCCATCTGCTCATGTTTACATTGCAATGGCAAAATTATTTGCTTG
TGATGAATGTTTCAAAGTTACCGATGATTCACTTCAATTATTTGGTGGTTATGGTTATCT
TAAAGATTATCCAGTTGAAAGATATTTACGTGATCTTCGTGTTCATCGTATTTTGGAAGG
TTCTGATGCTGTTATGAGATAATCATTTCAAGAGAATTAAGTAAAGA
Length of 3' end seq. 637
Connected seq. ID VFB804P
Connected seq. 
>VFB804P.Seq
TTTAATNTTGTNCCAAATCCAAGNGTTGGNTTAACAGAGGATCAAAANCAATTTCAATCA
ANGGCNTTAGATTTTGCNCAAGAAAAGATGAANCCNTTTGCAGAGAAANGGGATAAAGAG
GAATNTTTTCCAAGAGATGTGATGAGAGAA----------ATGGTCAGAACGGGCGCGGA
GAAGGGACCAAAGGGGATCTCCTGTCTCTTGGTGGAGAAGGACACCCCAGGCCTTAGCTT
CGGCAAAAAGGAGGAGAAACTAGGCTGGAACACCCAACCAACTCGTGCATTGATTTTCGA
AGATTGTCGTGTACCAGTTGGCAATTTAATTGGCAAAGAGGGTCAAGGCTTTTCCATTGC
TATGAACGCTTTAAACGGCGGTCGTATCAACATTGGTGCTTGTAGTTTGGGTGGGGCCCA
ATCTTGTTTGGTGGCCGCTAGAGATCATGTTAAAGTTAGAAAACAATTCAATCAACCATT
GGAACATTTCCAAGCCGTTCAATTTAAAATGGCAGATATGGCCACTAAATTACATGCATC
TCGTATTATGATTAGAAATGCCGCTGCAATGTTGGATGCAAAAGATCCATCTGCTCATGT
TTACATTGCAATGGCAAAATTATTTGCTTGTGATGAATGTTTCAAAGTTACCGATGATTC
ACTTCAATTATTTGGTGGTTATGGTTATCTTAAAGATTATCCAGTTGAAAGATATTTACG
TGATCTTCGTGTTCATCGTATTTTGGAAGGTTCTGATGCTGTTATGAGATAATCATTTCA
AGAGAATTAAGTAAAGA
Length of connected seq. 787
Full length Seq ID -
Full length Seq. -
Length of full length seq. -