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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFB785
Library VF
(Link to library)
Clone ID VFB785
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID VFB785P
(Link to Original site)
Representative DNA sequence 
>VFB785 (VFB785Q) /CSM/VF/VFB7-D/VFB785Q.Seq.d/
AACTTTAAAGAAAGAATAAACAACCATGGGTAAAGAGAAAACTCACATTAACATTGTCGT
CATTGGTCACGTAGATGCTGGTAAATCAACCACCACTGGTCATTTAATCTACAAATGTGG
TGGTATCGATAAGAGAGTTATCGAAAAGTATGAAAAGGAAGCTTCTGAAATGGGTAAACA
ATCCTTCAAATACGCCTGGGTTATGGATAAATTAAAGGCTGAACGTGAAAGAGGTATCAC
CATCGATATTGCTTTATGGAAATTCGAAACCTCCAAATACTACTTCACCATCATTGATGC
CCCAGGTCACAGAGATTTCATCAAGAATATGATTACTGGTACCTCCCAAGCCGATTGTGC
CGTCTTAGTCATTGCCTCCCCAACTGGTGAATTCGAAGCTGGTATCGCCAAAAACGGTCA
AACTCGTGAACACGCTCTCCTTGCTTACACTTTAGGTGTCAAACAAATGATCGTTGCTAT
CAACAAGATGGATGAAAAATCAACCAACTACXXXXXXXXXXCGAACCAAAAACGTCCACA
CGATAAACCATTACGTATTCCATTACAAGATGTCTACAAGATCGGTGGTATCGGTACTGT
TCCAGTAGGTCGTGTCGAAACTGGTATCATTAAACCAGGTATGGTCGTCACCTTTGCCCC
AGCTGGTCTCTCAACTGAAGTCAAATCAGTCGAAATGCATCACGAACAACTCCCAGAAGC
CCGTCCAGGTGACAATGTAGGTTTCAACGTTAAAAACGTTTCAGTCAAAGAAATTAAAAG
AGGTATGGTCGCTGGTGACTCCAAAAACGATCCACCACAAGAAACTGAAAAATTCGTTGC
TCAAGTTATCGTCCTCAACCATCCAGGTCAAATCCATGCTGGTTACTCACCAGTCTTAGA
TTGTCACACTGCTCACATTGCCTGTAAATTCACTGAAATCGTCGATAAAGTTGATCGTCG
TACTGGTGCCGTCGTTGCCAAAGAAGGTACTGCCGCCGTCGTCTTAAAGAATGGTGATGC
CGCTATGGTCGAATTAACCCCATCTCGTCCAATGTGTGTTGAATCCTTCACTGAATACCC
ACCATTAGGTCGTTTCGCCGTCAGAGATATGAGACAAACCGTCGCCGTCGGTGTCATCAA
ATCAACCGTCAAGAAAGCCCCAGGTAAAGCCGGTGATAAGAAAGGTGCTGCTGCCCCATC
AAAAAAGAAATAAATAAAAAATTTCTTAATAAATAGCATTTTAT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
tlkke*TTMGKEKTHINIVVIGHVDAGKSTTTGHLIYKCGGIDKRVIEKYEKEASEMGKQ
SFKYAWVMDKLKAERERGITIDIALWKFETSKYYFTIIDAPGHRDFIKNMITGTSQADCA
VLVIASPTGEFEAGIAKNGQTREHALLAYTLGVKQMIVAINKMDEKSTNY---

---NQKRPHDKPLRIPLQDVYKIGGIGTVPVGRVETGIIKPGMVVTFAPAGLSTEVKSVE
MHHEQLPEARPGDNVGFNVKNVSVKEIKRGMVAGDSKNDPPQETEKFVAQVIVLNHPGQI
HAGYSPVLDCHTAHIACKFTEIVDKVDRRTGAVVAKEGTAAVVLKNGDAAMVELTPSRPM
CVESFTEYPPLGRFAVRDMRQTVAVGVIKSTVKKAPGKAGDKKGAAAPSKKK*iknflin
sil
Translated Amino Acid sequence (All Frames) 
Frame A:
nfkerinnhg*rensh*hcrhwsrrcw*inhhwsfnlqmwwyr*esyrkv*kgsf*ng*t
ilqirlgyg*ikg*t*kryhhrycfmeirnlqillhhh*cprsqrfhqeydywylpsrlc
rlshclpnw*irswyrqkrsns*trspclhfrcqtndrcyqqdg*kinql---

---rtknvhtinhyvfhykmstrsvvsvlfq*vvsklvslnqvwssplpqlvsqlksnqs
kcitnnsqkpvqvtm*vstlktfqskklkevwslvtpktihhkklknsllklssstiqvk
smlvthqs*ivtlltlpvnslkssiklivvlvpslpkkvlppss*rmvmplwsn*phlvq
cvlnpslnthh*vvspsei*dkpspsvssnqpsrkpqvkpvirkvllphqkrnk*kis**
iafy

Frame B:
tlkke*TTMGKEKTHINIVVIGHVDAGKSTTTGHLIYKCGGIDKRVIEKYEKEASEMGKQ
SFKYAWVMDKLKAERERGITIDIALWKFETSKYYFTIIDAPGHRDFIKNMITGTSQADCA
VLVIASPTGEFEAGIAKNGQTREHALLAYTLGVKQMIVAINKMDEKSTNY---

---epktstr*titysitrclqdrwyrycssrscrnwyh*trygrhlcpswsln*sqisr
nasrttprspsr*qcrfqr*krfsqrn*krygrw*lqkrsttrn*kircssyrpqpsrsn
pcwlltslrlshcshcl*ih*nrr*s*ssywcrrcqrrycrrrlkew*crygrinpissn
vc*ilh*iptirsfrrqryetnrrrrchqinrqespr*sr**ercccpikkeinkkflnk
*hf

Frame C:
l*rknkqpwvkrkltltlsslvt*mlvnqpplvi*stnvvvsirelsksmkrkllkwvnn
psntpglwin*rlnvkevspsillygnskppnttspslmpqvteissri*llvppkpivp
s*slppqlvnsklvspktvklvntlslltl*vsnk*sllstrwmknqpt---

---NQKRPHDKPLRIPLQDVYKIGGIGTVPVGRVETGIIKPGMVVTFAPAGLSTEVKSVE
MHHEQLPEARPGDNVGFNVKNVSVKEIKRGMVAGDSKNDPPQETEKFVAQVIVLNHPGQI
HAGYSPVLDCHTAHIACKFTEIVDKVDRRTGAVVAKEGTAAVVLKNGDAAMVELTPSRPM
CVESFTEYPPLGRFAVRDMRQTVAVGVIKSTVKKAPGKAGDKKGAAAPSKKK*iknflin
sil
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFB785 (VFB785Q) /CSM/VF/VFB7-D/VFB785Q.Seq.d/                       2319   0.0
VFK740 (VFK740Q) /CSM/VF/VFK7-B/VFK740Q.Seq.d/                       1346   0.0
VFK563 (VFK563Q) /CSM/VF/VFK5-C/VFK563Q.Seq.d/                       1346   0.0
VFI810 (VFI810Q) /CSM/VF/VFI8-A/VFI810Q.Seq.d/                       1346   0.0
VFH520 (VFH520Q) /CSM/VF/VFH5-A/VFH520Q.Seq.d/                       1346   0.0
VFF439 (VFF439Q) /CSM/VF/VFF4-B/VFF439Q.Seq.d/                       1346   0.0
VFH764 (VFH764Q) /CSM/VF/VFH7-C/VFH764Q.Seq.d/                       1338   0.0
VFO616 (VFO616Q) /CSM/VF/VFO6-A/VFO616Q.Seq.d/                       1332   0.0
VFN376 (VFN376Q) /CSM/VF/VFN3-D/VFN376Q.Seq.d/                       1332   0.0
VFN329 (VFN329Q) /CSM/VF/VFN3-B/VFN329Q.Seq.d/                       1332   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

X55973|X55973.1 D. discoideum EF1-I gene for elongation factor 1 alpha.  1332  0.0  2
X55972|X55972.1 D. discoideum EF1-II gene for elongation factor 1 alpha.  1277  0.0  2
AF016242|AF016242.1 Dictyostelium discoideum protein synthesis elongation factor 1-alpha (tef2) gene, partial cds.  1104  0.0  3
M29935|M29935.1 C.albicans elongation factor 1-alpha (TEF-2) gene, complete cds.   264  0.0  8
M29934|M29934.1 C.albicans elongation factor 1-alpha (TEF-1) gene, complete cds.   192  0.0  9
AX109715|AX109715.1 Sequence 448 from Patent WO0123604.          147  e-158  8
AX109714|AX109714.1 Sequence 447 from Patent WO0123604.          147  e-158  8
AF402092|AF402092.1 Pichia anomala strain NRRL Y-366 translation elongation factor 1-alpha gene, partial cds.   151  e-158  8
AX109679|AX109679.1 Sequence 412 from Patent WO0123604.          161  e-151  8
AX109682|AX109682.1 Sequence 415 from Patent WO0123604.          161  e-150  8
dna update 2003. 9. 9
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

DQ138080_1(DQ138080|pid:none) Phytophthora parasitica isolate 14...   561   e-158
BC080974_1(BC080974|pid:none) Xenopus tropicalis hypothetical LO...   557   e-157
AJ249839_1(AJ249839|pid:none) Phytophthora infestans partial mRN...   555   e-157
AB073631_1(AB073631|pid:none) Salsola komarovii skef-1A mRNA for...   554   e-156
FB784741_1(FB784741|pid:none) Sequence 4014 from Patent WO200803...   550   e-155
AC026875_2(AC026875|pid:none) Genomic sequence for Arabidopsis t...   550   e-155
EU976709_1(EU976709|pid:none) Zea mays clone 988175 elongation f...   550   e-155
(P13905) RecName: Full=Elongation factor 1-alpha;          Short...   550   e-155
FB784713_1(FB784713|pid:none) Sequence 3986 from Patent WO200803...   550   e-155
BC071619_1(BC071619|pid:none) Homo sapiens eukaryotic translatio...   550   e-155
protein update 2009. 6.17
PSORT 

 psg: 0.84  gvh: 0.34  alm: 0.39  top: 0.53  tms: 0.00  mit: 0.19  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: cytoplasmic
	 36.0 %: nuclear
	 12.0 %: cytoskeletal
	  8.0 %: vacuolar
	  4.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for VFB785 is cyt
5' end seq. ID VFB785F
5' end seq. 
>VFB785F.Seq
AACTTTAAAGAAAGAATAAACAACCATGGGTAAAGAGAAAACTCACATTAACATTGTCGT
CATTGGTCACGTAGATGCTGGTAAATCAACCACCACTGGTCATTTAATCTACAAATGTGG
TGGTATCGATAAGAGAGTTATCGAAAAGTATGAAAAGGAAGCTTCTGAAATGGGTAAACA
ATCCTTCAAATACGCCTGGGTTATGGATAAATTAAAGGCTGAACGTGAAAGAGGTATCAC
CATCGATATTGCTTTATGGAAATTCGAAACCTCCAAATACTACTTCACCATCATTGATGC
CCCAGGTCACAGAGATTTCATCAAGAATATGATTACTGGTACCTCCCAAGCCGATTGTGC
CGTCTTAGTCATTGCCTCCCCAACTGGTGAATTCGAAGCTGGTATCGCCAAAAACGGTCA
AACTCGTGAACACGCTCTCCTTGCTTACACTTTAGGTGTCAAACAAATGATCGTTGCTAT
CAACAAGATGGATGAAAAATCAACCAACTAC----------
Length of 5' end seq. 511
3' end seq. ID VFB785Z
3' end seq. 
>VFB785Z.Seq
----------CGAACCAAAAACGTCCACACGATAAACCATTACGTATTCCATTACAAGAT
GTCTACAAGATCGGTGGTATCGGTACTGTTCCAGTAGGTCGTGTCGAAACTGGTATCATT
AAACCAGGTATGGTCGTCACCTTTGCCCCAGCTGGTCTCTCAACTGAAGTCAAATCAGTC
GAAATGCATCACGAACAACTCCCAGAAGCCCGTCCAGGTGACAATGTAGGTTTCAACGTT
AAAAACGTTTCAGTCAAAGAAATTAAAAGAGGTATGGTCGCTGGTGACTCCAAAAACGAT
CCACCACAAGAAACTGAAAAATTCGTTGCTCAAGTTATCGTCCTCAACCATCCAGGTCAA
ATCCATGCTGGTTACTCACCAGTCTTAGATTGTCACACTGCTCACATTGCCTGTAAATTC
ACTGAAATCGTCGATAAAGTTGATCGTCGTACTGGTGCCGTCGTTGCCAAAGAAGGTACT
GCCGCCGTCGTCTTAAAGAATGGTGATGCCGCTATGGTCGAATTAACCCCATCTCGTCCA
ATGTGTGTTGAATCCTTCACTGAATACCCACCATTAGGTCGTTTCGCCGTCAGAGATATG
AGACAAACCGTCGCCGTCGGTGTCATCAAATCAACCGTCAAGAAAGCCCCAGGTAAAGCC
GGTGATAAGAAAGGTGCTGCTGCCCCATCAAAAAAGAAATAAATAAAAAATTTCTTAATA
AATAGCATTTTAT
Length of 3' end seq. 723
Connected seq. ID VFB785P
Connected seq. 
>VFB785P.Seq
AACTTTAAAGAAAGAATAAACAACCATGGGTAAAGAGAAAACTCACATTAACATTGTCGT
CATTGGTCACGTAGATGCTGGTAAATCAACCACCACTGGTCATTTAATCTACAAATGTGG
TGGTATCGATAAGAGAGTTATCGAAAAGTATGAAAAGGAAGCTTCTGAAATGGGTAAACA
ATCCTTCAAATACGCCTGGGTTATGGATAAATTAAAGGCTGAACGTGAAAGAGGTATCAC
CATCGATATTGCTTTATGGAAATTCGAAACCTCCAAATACTACTTCACCATCATTGATGC
CCCAGGTCACAGAGATTTCATCAAGAATATGATTACTGGTACCTCCCAAGCCGATTGTGC
CGTCTTAGTCATTGCCTCCCCAACTGGTGAATTCGAAGCTGGTATCGCCAAAAACGGTCA
AACTCGTGAACACGCTCTCCTTGCTTACACTTTAGGTGTCAAACAAATGATCGTTGCTAT
CAACAAGATGGATGAAAAATCAACCAACTAC----------CGAACCAAAAACGTCCACA
CGATAAACCATTACGTATTCCATTACAAGATGTCTACAAGATCGGTGGTATCGGTACTGT
TCCAGTAGGTCGTGTCGAAACTGGTATCATTAAACCAGGTATGGTCGTCACCTTTGCCCC
AGCTGGTCTCTCAACTGAAGTCAAATCAGTCGAAATGCATCACGAACAACTCCCAGAAGC
CCGTCCAGGTGACAATGTAGGTTTCAACGTTAAAAACGTTTCAGTCAAAGAAATTAAAAG
AGGTATGGTCGCTGGTGACTCCAAAAACGATCCACCACAAGAAACTGAAAAATTCGTTGC
TCAAGTTATCGTCCTCAACCATCCAGGTCAAATCCATGCTGGTTACTCACCAGTCTTAGA
TTGTCACACTGCTCACATTGCCTGTAAATTCACTGAAATCGTCGATAAAGTTGATCGTCG
TACTGGTGCCGTCGTTGCCAAAGAAGGTACTGCCGCCGTCGTCTTAAAGAATGGTGATGC
CGCTATGGTCGAATTAACCCCATCTCGTCCAATGTGTGTTGAATCCTTCACTGAATACCC
ACCATTAGGTCGTTTCGCCGTCAGAGATATGAGACAAACCGTCGCCGTCGGTGTCATCAA
ATCAACCGTCAAGAAAGCCCCAGGTAAAGCCGGTGATAAGAAAGGTGCTGCTGCCCCATC
AAAAAAGAAATAAATAAAAAATTTCTTAATAAATAGCATTTTAT
Length of connected seq. 1234
Full length Seq ID -
Full length Seq. -
Length of full length seq. -