>VFB779 (VFB779Q) /CSM/VF/VFB7-D/VFB779Q.Seq.d/
ACTGTTGGCCTACTGGGTAGTTATTTAAGTAATATTTCACACAATATATAAAATGTCATC
TTATTTATTCACTAGTGAATCCGTCACCGAAGGTCATCCAGATAAAATCTGTGATCAAGT
ATCAGATGCTGTTCTCGATGCTTGTTTAGCTCAAGATCCATTATCAAAAGTTGCTTGTGA
AACTGCAACCAAAACTGGTATGGTTATGATTTTAGGTGAAATTACCACCAAAGCCAACGT
TGACTATCAAAAAGTTGNTCGTGAAGCTGNNAAAAAGATTGGTTATGATGACTCATCAAA
AGGTNNTGATTACAAAACTTGCAATGNCXXXXXXXXXXTNTACCATTTGAATCCATCAGG
TCGTTTNGTCATTGGTGGCCCAANGGGTGATTNTGGTTTAACTGGTNGTAANATCATCAT
TGATTCATANGGTGGTTGGGGNGCTCNCGGTGGTGGNGCTTTNTCTGGTAAAAATGCCNC
TAAAGTCGATCGTTNTGGNGCNTACGCTGCTANANGGATCGCTAAATCTTTAGTTGCCNC
TGGTCTTNCCNATCGTTGTTTAGTTCAANTTTCANACTCTATTGGNGNTGCCAANCCNCT
CAGNGTCTTTGTTNANACCTATGGGACCGGTAAAAANACTGANGCTGAAANCCNTGCCAT
CATCGATAANAACTTTGATTTACGNCCAGGTTGTTTAANGNGTGACCCCCAATTAACTCG
NCCNATTTTCCAAAAAACNG

llaywvvi*VIFHTIYKMSSYLFTSESVTEGHPDKICDQVSDAVLDACLAQDPLSKVACE
TATKTGMVMILGEITTKANVDYQKVXREAXKKIGYDDSSKGXDYKTCNX---

---YHLNPSGRXVIGGPXGDXGLTGXXIIIDSXGGWGAXGGGAXSGKNAXKVDRXGAYAA
XXIAKSLVAXGLXXRCLVQXSXSIGXAXPLXVFVXTYGTGKXTXAEXXAIIDXNFDLRPG
CLXXDPQLTRPIFQKT

Frame A:
tvgllgsylsnishni*nvilfih**irhrrssr*nl*ssirccsrclfssrsiikscl*
ncnqnwygydfr*nyhqsqr*lsksxs*sxxkdwl**likrx*lqnlqx---

---xti*ihqvvxslvaqxvixv*lvvxsslihxvvgxlxvvxlxlvkmplksivxxxtl
lxgslnl*lplvxpivv*fxfxtllxxpxxsxslxxpmgpvkxlxlkxxpssixtliyxq
vv*xvtpn*lxxfskkx

Frame B:
llaywvvi*VIFHTIYKMSSYLFTSESVTEGHPDKICDQVSDAVLDACLAQDPLSKVACE
TATKTGMVMILGEITTKANVDYQKVXREAXKKIGYDDSSKGXDYKTCNX---

---xpfesirsfxhwwpxg*xwfnwx*xhh*fixwlgxsrwwxfxw*kcx*srsxwxxrc
xxdr*ifscxwsxxslfssxfxlywxcqxxqxlcxxlwdr*kx*x*xpchhr*xl*ftxr
lfxx*ppinsxxfpkn

Frame C:
cwptg*lfk*yftqyikchliyslvnpspkviqiksvikyqmlfsmlv*lkihyqkllvk
lqpklvwl*f*vklppkptltikklxvklxkrlvmmthqkvxitklam---

---YHLNPSGRXVIGGPXGDXGLTGXXIIIDSXGGWGAXGGGAXSGKNAXKVDRXGAYAA
XXIAKSLVAXGLXXRCLVQXSXSIGXAXPLXVFVXTYGTGKXTXAEXXAIIDXNFDLRPG
CLXXDPQLTRPIFQKT

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFB779 (VFB779Q) /CSM/VF/VFB7-D/VFB779Q.Seq.d/                       1136   0.0
SFG116 (SFG116Q) /CSM/SF/SFG1-A/SFG116Q.Seq.d/                        617   e-175
VFH824 (VFH824Q) /CSM/VF/VFH8-A/VFH824Q.Seq.d/                        603   e-171
VFL654 (VFL654Q) /CSM/VF/VFL6-C/VFL654Q.Seq.d/                        591   e-168
VFC217 (VFC217Q) /CSM/VF/VFC2-A/VFC217Q.Seq.d/                        589   e-167
VFO332 (VFO332Q) /CSM/VF/VFO3-B/VFO332Q.Seq.d/                        585   e-166
VFO270 (VFO270Q) /CSM/VF/VFO2-C/VFO270Q.Seq.d/                        585   e-166
VFO116 (VFO116Q) /CSM/VF/VFO1-A/VFO116Q.Seq.d/                        585   e-166
VFN766 (VFN766Q) /CSM/VF/VFN7-C/VFN766Q.Seq.d/                        585   e-166
VFN346 (VFN346Q) /CSM/VF/VFN3-B/VFN346Q.Seq.d/                        585   e-166

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AX595308|AX595308.1 Sequence 962 from Patent EP1258494.          109  4e-21  2
M23368|M23368.1 Saccharomyces cerevisiae S-adenosylmethionine synthetase (SAM2) gene, complete cds.   109  7e-21  2
AJ390497|AJ390497.1 Candida albicans partial mRNA for S-adenosylmethionine synthetase 2 (sam2 gene).   107  2e-19  1
U33057|U33057.1 Saccharomyces cerevisiae chromosome IV cosmids 8166, 9787, 9717, and lambda 3073.   109  2e-19  2
T37377|T37377.1 EST102487 S. cerevisiae strain X2180-1A Saccharomyces cerevisiae cDNA 5' end, mRNA sequence.    70  1e-16  3
BF779786|BF779786.1 3234-76 hindgut and Malpighian tubule cDNA library Ctenocephalides felis cDNA clone 3234-76, mRNA sequence.    46  9e-15  4
BD281902|BD281902.1 Flea head, nerve cord, hindgut and malpighian tubule nucleic acid molecules, proteins and uses thereof.    46  9e-15  4
BI936695|BI936695.1 dc15f10.y1 NICHD XGC Li1 Xenopus laevis cDNA clone IMAGE:3397195 5' similar to SW:METK_RAT P18298 S-ADENOSYLMETHIONINE SYNTHETASE GAMMA FORM ;, mRNA sequence.    48  1e-11  3
AL425274|AL425274.1 clone BA0AB004F05 of library BA0AB from strain CLIB 210 of  Kluyveromyces lactis.    80  4e-11  1
BJ059481|BJ059481.1 Xenopus laevis cDNA clone:XL062l20, 5' end, single read.    48  8e-11  3

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54F07) RecName: Full=S-adenosylmethionine synthetase;         ...   179   8e-44
(P50304) RecName: Full=S-adenosylmethionine synthetase;         ...   155   9e-37
CP000496_283(CP000496|pid:none) Pichia stipitis CBS 6054 chromos...   155   2e-36
DQ098647_1(DQ098647|pid:none) Synthetic construct AdoMet synthet...   154   2e-36
(P19358) RecName: Full=S-adenosylmethionine synthetase 2;       ...   154   2e-36
(P10659) RecName: Full=S-adenosylmethionine synthetase 1;       ...   154   3e-36
J03477_1(J03477|pid:none) Yeast (S.cerevisiae) S-adenosylmethion...   154   3e-36
AE017341_393(AE017341|pid:none) Cryptococcus neoformans var. neo...   152   1e-35
CR382137_600(CR382137|pid:none) Debaryomyces hansenii strain CBS...   152   1e-35
AP007169_295(AP007169|pid:none) Aspergillus oryzae RIB40 genomic...   151   2e-35

 psg: 0.88  gvh: 0.51  alm: 0.43  top: 0.37  tms: 0.00  mit: 0.37  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 28.0 %: cytoplasmic
	 20.0 %: extracellular, including cell wall
	 20.0 %: mitochondrial
	 16.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: peroxisomal
	  4.0 %: endoplasmic reticulum

>> prediction for VFB779 is cyt

>VFB779F.Seq
ACTGTTGGCCTACTGGGTAGTTATTTAAGTAATATTTCACACAATATATAAAATGTCATC
TTATTTATTCACTAGTGAATCCGTCACCGAAGGTCATCCAGATAAAATCTGTGATCAAGT
ATCAGATGCTGTTCTCGATGCTTGTTTAGCTCAAGATCCATTATCAAAAGTTGCTTGTGA
AACTGCAACCAAAACTGGTATGGTTATGATTTTAGGTGAAATTACCACCAAAGCCAACGT
TGACTATCAAAAAGTTGNTCGTGAAGCTGNNAAAAAGATTGGTTATGATGACTCATCAAA
AGGTNNTGATTACAAAACTTGCAATGNC----------

>VFB779Z.Seq
----------TNTACCATTTGAATCCATCAGGTCGTTTNGTCATTGGTGGCCCAANGGGT
GATTNTGGTTTAACTGGTNGTAANATCATCATTGATTCATANGGTGGTTGGGGNGCTCNC
GGTGGTGGNGCTTTNTCTGGTAAAAATGCCNCTAAAGTCGATCGTTNTGGNGCNTACGCT
GCTANANGGATCGCTAAATCTTTAGTTGCCNCTGGTCTTNCCNATCGTTGTTTAGTTCAA
NTTTCANACTCTATTGGNGNTGCCAANCCNCTCAGNGTCTTTGTTNANACCTATGGGACC
GGTAAAAANACTGANGCTGAAANCCNTGCCATCATCGATAANAACTTTGATTTACGNCCA
GGTTGTTTAANGNGTGACCCCCAATTAACTCGNCCNATTTTCCAAAAAACNG

>VFB779P.Seq
ACTGTTGGCCTACTGGGTAGTTATTTAAGTAATATTTCACACAATATATAAAATGTCATC
TTATTTATTCACTAGTGAATCCGTCACCGAAGGTCATCCAGATAAAATCTGTGATCAAGT
ATCAGATGCTGTTCTCGATGCTTGTTTAGCTCAAGATCCATTATCAAAAGTTGCTTGTGA
AACTGCAACCAAAACTGGTATGGTTATGATTTTAGGTGAAATTACCACCAAAGCCAACGT
TGACTATCAAAAAGTTGNTCGTGAAGCTGNNAAAAAGATTGGTTATGATGACTCATCAAA
AGGTNNTGATTACAAAACTTGCAATGNC----------TNTACCATTTGAATCCATCAGG
TCGTTTNGTCATTGGTGGCCCAANGGGTGATTNTGGTTTAACTGGTNGTAANATCATCAT
TGATTCATANGGTGGTTGGGGNGCTCNCGGTGGTGGNGCTTTNTCTGGTAAAAATGCCNC
TAAAGTCGATCGTTNTGGNGCNTACGCTGCTANANGGATCGCTAAATCTTTAGTTGCCNC
TGGTCTTNCCNATCGTTGTTTAGTTCAANTTTCANACTCTATTGGNGNTGCCAANCCNCT
CAGNGTCTTTGTTNANACCTATGGGACCGGTAAAAANACTGANGCTGAAANCCNTGCCAT
CATCGATAANAACTTTGATTTACGNCCAGGTTGTTTAANGNGTGACCCCCAATTAACTCG
NCCNATTTTCCAAAAAACNG