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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFB770
Library VF
(Link to library)
Clone ID VFB770
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16382-1
Original site URL
Representative seq. ID VFB770E
(Link to Original site)
Representative DNA sequence 
>VFB770 (VFB770Q) /CSM/VF/VFB7-C/VFB770Q.Seq.d/
ATAAATCATTTAAAAATGGACGGTGAAGATGTTCAAGCTTTAGTTATCGATAACGGTTCT
GGTATGTGTAAAGCCGGTTTTGCTGGTGATGATGCCCCACGTGCTGTTTTCCCATCAATT
GTTGGTCGTCCAAGACACACTGGTGTTATGGTCGGTATGGGTCAAAAAGACTCATATGTA
GGTGATGAAGCCCAATCAAAGAGAGGTATCTTAACCCTCAAATACCCAATTGAACACGGT
ATCGTTACCAACTGGGATGATATGGAAAAAATCTGGCATCATACTTTCTACAATGAACTC
CGTGTTGCCCCAGAAGAACACCCAGTTCTCTTAACTGAAGCCCCATTAAATCCAAAGGCC
AACAGAGAAAAAATGACCCAAATTATGTTTGAAACCTTCAACACCCCAGCCATGTACGTC
GCCATNNAAGCCGTCTTATCCTTATATGCCTCTGGTCGTACCACCGGTATTGTTATGGAT
TCAGGTGATGGTGTCTCCCACACTGTACCAATCTATGAAGGTTATGCTTTACCACATGCC
ATCTTACGTTTAGATTTAGCTGGTCGTGATCTTACCGATTACATGATGAAAATCTTAACT
GAACGTGGTTACTCATTCACCACCACTGCCGAAAGAGAAATCGTCAGAGATATCAAAGAA
AAATTAGCCTACGTCGCCCTCGACTTTGAAGCTGAAATGCAAACTGCTGCCTCATCATCA
GCCCTCGAAAAATCATACGAATTACCAGACGGTCAAGTCATCACCATCGGTAATGAACGT
TTCCGTTGCCCAGAAGCCCTCTTCCAACCATCATTCTTAGGTATGGAATCTGCTGGTATC
CACGAAACCACCTACAACTCCATCATGAAATGTGATGTTGATATCCGTAAAGATTTATAC
GGTAACGTCGTCTTATCCGGTGGTACAACTATGTTCCCAGGTATTGCTGATCGTATGAAC
AAAGAATTAACTGCTTTAGCCCCATCAACCATGAAAATTAAAATCATTGCTCCACCAGAA
CGTAAATACTCTGTCTGGATTGGTGGATCTATCTTAGCTTCACTCTCAACTTTCCAACAA
ATGTGGATCTCCAAAGAAGAATACGACGAATCAGGTCCATCAATTGTTCACAGAAAATGT
TTCTAAATTATTAATAAAAATAAAAAG
sequence update 2001. 6. 9
Translated Amino Acid sequence 
INHLKMDGEDVQALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDSYV
GDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKA
NREKMTQIMFETFNTPAMYVAXXAVLSLYASGRTTGIVMDSGDGVSHTVPIYEGYALPHA
ILRLDLAGRDLTDYMMKILTERGYSFTTTAEREIVRDIKEKLAYVALDFEAEMQTAASSS
ALEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESAGIHETTYNSIMKCDVDIRKDLY
GNVVLSGGTTMFPGIADRMNKELTALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQ
MWISKEEYDESGPSIVHRKCF*iinknkk
Translated Amino Acid sequence (All Frames) 
Frame A:
INHLKMDGEDVQALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDSYV
GDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKA
NREKMTQIMFETFNTPAMYVAXXAVLSLYASGRTTGIVMDSGDGVSHTVPIYEGYALPHA
ILRLDLAGRDLTDYMMKILTERGYSFTTTAEREIVRDIKEKLAYVALDFEAEMQTAASSS
ALEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESAGIHETTYNSIMKCDVDIRKDLY
GNVVLSGGTTMFPGIADRMNKELTALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQ
MWISKEEYDESGPSIVHRKCF*iinknkk


Frame B:
*ii*kwtvkmfkl*lsitvlvcvkpvllvmmphvlfshqllvvqdtlvlwsvwvkkthm*
vmkpnqrevs*psntqlntvslptgmiwkksgiilstmnsvlpqkntqfs*lkph*iqrp
tekk*pklclkpstpqpctspxkpsypymplvvppvllwiqvmvsptlyqsmkvmlyhmp
syv*i*lvvilpit**ks*lnvvthspplpkeksseiskkn*ptspstlklkckllphhq
psknhtnyqtvksspsvmnvsvaqkpssnhhs*vwnllvstkppttps*nvmlisvkiyt
vtssypvvqlcsqvlliv*tkn*ll*phqp*klksllhqnvntlsglvdls*lhsqlsnk
cgspkknttnqvhqlftenvskllikik


Frame C:
ksfkngr*rcssfsyr*rfwyv*srfcw**cptccfpincwsskthwcygrygskrlicr
**spikerylnpqipn*tryryqlg*ygknlasyflq*tpccprrtpssln*spikskgq
qrkndpnyv*nlqhpshvrrhxsrliliclwsyhrycygfr*wclphctnl*rlcfttch
ltfrfsws*syrlhdenln*twllihhhcrkrnrqryqrkislrrprl*s*nanccliis
prkiiritrrsshhhr**tfplprsplptiilrygicwyprnhlqlhhem*c*yp*rfir
*rrlirwynyvpryc*syeqrincfspinhen*nhcstrt*ilcldwwiylsftlnfptn
vdlqrrirrirsincsqkmflny**k*k
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFB770 (VFB770Q) /CSM/VF/VFB7-C/VFB770Q.Seq.d/                       2302   0.0
VFC585 (VFC585Q) /CSM/VF/VFC5-D/VFC585Q.Seq.d/                       2288   0.0
VFL458 (VFL458Q) /CSM/VF/VFL4-C/VFL458Q.Seq.d/                       2286   0.0
VFL313 (VFL313Q) /CSM/VF/VFL3-A/VFL313Q.Seq.d/                       2286   0.0
VFK736 (VFK736Q) /CSM/VF/VFK7-B/VFK736Q.Seq.d/                       2286   0.0
VFI775 (VFI775Q) /CSM/VF/VFI7-D/VFI775Q.Seq.d/                       2286   0.0
VFI660 (VFI660Q) /CSM/VF/VFI6-C/VFI660Q.Seq.d/                       2286   0.0
VFG739 (VFG739Q) /CSM/VF/VFG7-B/VFG739Q.Seq.d/                       2286   0.0
VFF194 (VFF194Q) /CSM/VF/VFF1-D/VFF194Q.Seq.d/                       2286   0.0
VFE624 (VFE624Q) /CSM/VF/VFE6-A/VFE624Q.Seq.d/                       2286   0.0
own update 2004. 8. 9
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

X03281|X03281.1 Dictyostelium discoideum gene for actin A8.     2230  0.0  1
AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.  2103  0.0  3
M14146|M14146.1 D.discoideum actin 15 gene, complete cds.       2030  0.0  1
AC115579|AC115579.2 Dictyostelium discoideum chromosome 2 map 4915084-5005461 strain AX4, complete sequence.  2030  0.0  3
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.  1842  0.0  3
AC115577|AC115577.2 Dictyostelium discoideum chromosome 2 map 4657875-4914984 strain AX4, complete sequence.  1754  0.0  15
AC116956|AC116956.2 Dictyostelium discoideum chromosome 2 map 1418423-1684967 strain AX4, complete sequence.  1665  0.0  8
X03282|X03282.1 dictyostelium discoideum gene for actin A12.    1612  0.0  1
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.  1495  0.0  3
U25660|U25660.1 Dictyostelium discoideum actin gene, partial cds.  1368  0.0  1
dna update 2003. 9. 9
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54H30) RecName: Full=Major actin; AltName: Full=Actin-1; AltNa...   754   0.0
(Q553U6) RecName: Full=Putative actin-22;  &AC116305_3(AC116305|...   750   0.0
ATDO(A93223;A92871;S67999;A03004)actin - slime mold (Dictyosteli...   748   0.0
B23412(B23412)actin 12 - slime mold (Dictyostelium discoideum)        748   0.0
(P02576) RecName: Full=Actin, plasmodial isoform;  &ATFY(A29044;...   747   0.0
X03282_1(X03282|pid:none) dictyostelium discoideum gene for acti...   746   0.0
(P07829) RecName: Full=Actin-3; AltName: Full=Actin-3-sub 1;          737   0.0
AY294154_1(AY294154|pid:none) Thecamoeba similis actin mRNA, com...   734   0.0
(P02578) RecName: Full=Actin-1;  &ATAX(A92886;A03004) &V00002_1(...   733   0.0
AF082863_1(AF082863|pid:none) Dreissena polymorpha cytoplasmic a...   726   0.0
protein update 2009. 7.10
PSORT 

 psg: 0.75  gvh: 0.34  alm: 0.44  top: 0.53  tms: 0.00  mit: 0.15  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: nuclear
	 32.0 %: cytoplasmic
	 20.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for VFB770 is nuc
5' end seq. ID VFB770F
5' end seq. 
>VFB770F.Seq
ATAAATCATTTAAAAATGGACGGTGAAGATGTTCAAGCTTTAGTTATCGATAACGGTTCT
GGTATGTGTAAAGCCGGTTTTGCTGGTGATGATGCCCCACGTGCTGTTTTCCCATCAATT
GTTGGTCGTCCAAGACACACTGGTGTTATGGTCGGTATGGGTCAAAAAGACTCATATGTA
GGTGATGAAGCCCAATCAAAGAGAGGTATCTTAACCCTCAAATACCCAATTGAACACGGT
ATCGTTACCAACTGGGATGATATGGAAAAAATCTGGCATCATACTTTCTACAATGAACTC
CGTGTTGCCCCAGAAGAACACCCAGTTCTCTTAACTGAAGCCCCATTAAATCCAAAGGCC
AACAGAGAAAAAATGACCCAAATTATGTTTGAAACCTTCAACACCCCAGCCATGTACGTC
GCCATNNAAGCCGTCTTATCCTTATATGCCTCTGGTCGTACCACCGGTATTGTTATGGAT
TCAGGTGATGGTGTCTCCCACACTGTACCAATCTATGAAGGTTATGCTTTACCACATGCC
ATCTTACGTTTAGATTTANC----------
Length of 5' end seq. 560
3' end seq. ID VFB770Z
3' end seq. 
>VFB770Z.Seq
----------TGTCTCCCACACTGTACCAATCTATGAAGGTTATGCTTTACCACATGCCA
TCTTACGTTTAGATTTAGCTGGTCGTGATCTTACCGATTACATGATGAAAATCTTAACTG
AACGTGGTTACTCATTCACCACCACTGCCGAAAGAGAAATCGTCAGAGATATCAAAGAAA
AATTAGCCTACGTCGCCCTCGACTTTGAAGCTGAAATGCAAACTGCTGCCTCATCATCAG
CCCTCGAAAAATCATACGAATTACCAGACGGTCAAGTCATCACCATCGGTAATGAACGTT
TCCGTTGCCCAGAAGCCCTCTTCCAACCATCATTCTTAGGTATGGAATCTGCTGGTATCC
ACGAAACCACCTACAACTCCATCATGAAATGTGATGTTGATATCCGTAAAGATTTATACG
GTAACGTCGTCTTATCCGGTGGTACAACTATGTTCCCAGGTATTGCTGATCGTATGAACA
AAGAATTAACTGCTTTAGCCCCATCAACCATGAAAATTAAAATCATTGCTCCACCAGAAC
GTAAATACTCTGTCTGGATTGGTGGATCTATCTTAGCTTCACTCTCAACTTTCCAACAAA
TGTGGATCTCCAAAGAAGAATACGACGAATCAGGTCCATCAATTGTTCACAGAAAATGTT
TCTAAATTATTAATAAAAATAAAAAG
Length of 3' end seq. 676
Connected seq. ID VFB770P
Connected seq. 
>VFB770P.Seq
ATAAATCATTTAAAAATGGACGGTGAAGATGTTCAAGCTTTAGTTATCGATAACGGTTCT
GGTATGTGTAAAGCCGGTTTTGCTGGTGATGATGCCCCACGTGCTGTTTTCCCATCAATT
GTTGGTCGTCCAAGACACACTGGTGTTATGGTCGGTATGGGTCAAAAAGACTCATATGTA
GGTGATGAAGCCCAATCAAAGAGAGGTATCTTAACCCTCAAATACCCAATTGAACACGGT
ATCGTTACCAACTGGGATGATATGGAAAAAATCTGGCATCATACTTTCTACAATGAACTC
CGTGTTGCCCCAGAAGAACACCCAGTTCTCTTAACTGAAGCCCCATTAAATCCAAAGGCC
AACAGAGAAAAAATGACCCAAATTATGTTTGAAACCTTCAACACCCCAGCCATGTACGTC
GCCATNNAAGCCGTCTTATCCTTATATGCCTCTGGTCGTACCACCGGTATTGTTATGGAT
TCAGGTGATGGTGTCTCCCACACTGTACCAATCTATGAAGGTTATGCTTTACCACATGCC
ATCTTACGTTTAGATTTANC----------TGTCTCCCACACTGTACCAATCTATGAAGG
TTATGCTTTACCACATGCCATCTTACGTTTAGATTTAGCTGGTCGTGATCTTACCGATTA
CATGATGAAAATCTTAACTGAACGTGGTTACTCATTCACCACCACTGCCGAAAGAGAAAT
CGTCAGAGATATCAAAGAAAAATTAGCCTACGTCGCCCTCGACTTTGAAGCTGAAATGCA
AACTGCTGCCTCATCATCAGCCCTCGAAAAATCATACGAATTACCAGACGGTCAAGTCAT
CACCATCGGTAATGAACGTTTCCGTTGCCCAGAAGCCCTCTTCCAACCATCATTCTTAGG
TATGGAATCTGCTGGTATCCACGAAACCACCTACAACTCCATCATGAAATGTGATGTTGA
TATCCGTAAAGATTTATACGGTAACGTCGTCTTATCCGGTGGTACAACTATGTTCCCAGG
TATTGCTGATCGTATGAACAAAGAATTAACTGCTTTAGCCCCATCAACCATGAAAATTAA
AATCATTGCTCCACCAGAACGTAAATACTCTGTCTGGATTGGTGGATCTATCTTAGCTTC
ACTCTCAACTTTCCAACAAATGTGGATCTCCAAAGAAGAATACGACGAATCAGGTCCATC
AATTGTTCACAGAAAATGTTTCTAAATTATTAATAAAAATAAAAAG
Length of connected seq. 1236
Full length Seq ID VFB770E
Full length Seq. 
>VFB770E.Seq
ATAAATCATTTAAAAATGGACGGTGAAGATGTTCAAGCTTTAGTTATCGATAACGGTTCT
GGTATGTGTAAAGCCGGTTTTGCTGGTGATGATGCCCCACGTGCTGTTTTCCCATCAATT
GTTGGTCGTCCAAGACACACTGGTGTTATGGTCGGTATGGGTCAAAAAGACTCATATGTA
GGTGATGAAGCCCAATCAAAGAGAGGTATCTTAACCCTCAAATACCCAATTGAACACGGT
ATCGTTACCAACTGGGATGATATGGAAAAAATCTGGCATCATACTTTCTACAATGAACTC
CGTGTTGCCCCAGAAGAACACCCAGTTCTCTTAACTGAAGCCCCATTAAATCCAAAGGCC
AACAGAGAAAAAATGACCCAAATTATGTTTGAAACCTTCAACACCCCAGCCATGTACGTC
GCCATNNAAGCCGTCTTATCCTTATATGCCTCTGGTCGTACCACCGGTATTGTTATGGAT
TCAGGTGATGGTGTCTCCCACACTGTACCAATCTATGAAGGTTATGCTTTACCACATGCC
ATCTTACGTTTAGATTTAGCTGGTCGTGATCTTACCGATTACATGATGAAAATCTTAACT
GAACGTGGTTACTCATTCACCACCACTGCCGAAAGAGAAATCGTCAGAGATATCAAAGAA
AAATTAGCCTACGTCGCCCTCGACTTTGAAGCTGAAATGCAAACTGCTGCCTCATCATCA
GCCCTCGAAAAATCATACGAATTACCAGACGGTCAAGTCATCACCATCGGTAATGAACGT
TTCCGTTGCCCAGAAGCCCTCTTCCAACCATCATTCTTAGGTATGGAATCTGCTGGTATC
CACGAAACCACCTACAACTCCATCATGAAATGTGATGTTGATATCCGTAAAGATTTATAC
GGTAACGTCGTCTTATCCGGTGGTACAACTATGTTCCCAGGTATTGCTGATCGTATGAAC
AAAGAATTAACTGCTTTAGCCCCATCAACCATGAAAATTAAAATCATTGCTCCACCAGAA
CGTAAATACTCTGTCTGGATTGGTGGATCTATCTTAGCTTCACTCTCAACTTTCCAACAA
ATGTGGATCTCCAAAGAAGAATACGACGAATCAGGTCCATCAATTGTTCACAGAAAATGT
TTCTAAATTATTAATAAAAATAAAAAG
Length of full length seq. 1167