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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFB769
Library VF
(Link to library)
Clone ID VFB769
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15456-1
Original site URL
Representative seq. ID VFB769P
(Link to Original site)
Representative DNA sequence 
>VFB769 (VFB769Q) /CSM/VF/VFB7-C/VFB769Q.Seq.d/
CTTTCATTTTACGTTGAGGGTATGTGATCCGAATCATTTGTGTGGATCATGAATTTATGG
CTAGAGTTTTTCGAATCTAAAGTTCGCCTCAACACTTTAAAAAGATGAATGAGACAAATA
TAGGTATTTCATAGTGCATATTAATTATCCAAGCGATTTTTATGCCTCCTAATGACCTAC
ATCTAATTGTCACCTGACTTACATAAAAGAAAATGAAATTACTTATTTTAACTTTATTTT
TAGCTACTATTGTTTTAGCTCAAGCTTTAACAGTACCATTAAACTTCCATCAAGCTTCAA
GAGAATCTAGAAGAAGAGTTCCACAAAAATGGTCAAACAGATTATCTGCTCTCAATGCTG
GTACCACAATCCCAATTTCAGATTTTGAAGATGCTCAATACTATGGTGCCATTACCATTG
GTACCCCAGGTCAAGCCTTCAAAGTAGTTTTCGATACTGGTTCATCCAACTTGTGGATTC
CATCAAAGAAATGTCCAATCACTGTTGTTGCATGTGATTTACATAACAAATATAACAGCG
GTGCCTCAAGCACATATGTCGCCAACGGAACTGATTTCACCATCCAATACGGTAGTGGTG
CTATGTCAGGTTTTGTCTCTCAAGAXXXXXXXXXXCTTGCTTTCCAATCCATCTCTGTTA
ATTCAATTCCACCAGTCTTTTACAACATGTTATCACAAGGTTTAGTTTCATCAACACTCT
TCTCCTTCTGGTTATCAAGAACTCCAGGTGCCAACGGTGGTGAACTCTCATTCGGTTCAA
TCGATAACACCAAATACACTGGTGACATTACCTACGTCCCATTAACCAACGAAACCTATT
GGGAATTCGTTATGGATGACTTTGCTATCGATGGTCAATCAGCTGGTTTCTGTGGTACTA
CTTGTCACGCAATTTGCGATTCAGGTACATCACTCATTGCTGGTCCAATGGCTGATATTA
CTGCCCTCAATGAAAAATTAGGTGCTGTCATCTTAAATGGTGAAGGTGTCTTCTCTGATT
GTAGCGTTATCAACACCTTACCAAATGTTACCATCACCGTTGCTGGTCGTGAATTTGTTT
TAACTCCAAAAGAATACGTTTTAGAAGTTACTGAGTTCGGAAAGACTGAATGTTTGAGTG
GATTTATGGGTATCGAGTTAAACATGGGAAATTTCTGGATCCTTGGTGATGTTTTCATCT
CTGCTTACTATACTGTATTCGATTTTGGTAATAAACAAGTTGGTTTCGCAACTGCCATTC
AAGGTTAAATTTTTTTAATTAATTTATATTTAAGATAGAAATAAAACTAAATAATAGAAC
AATAT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
filr*gyviriicvdhefmarvfri*sspqhfkkmnetnigis*ciliiqaifmppndlh
livt*lt*KKMKLLILTLFLATIVLAQALTVPLNFHQASRESRRRVPQKWSNRLSALNAG
TTIPISDFEDAQYYGAITIGTPGQAFKVVFDTGSSNLWIPSKKCPITVVACDLHNKYNSG
ASSTYVANGTDFTIQYGSGAMSGFVSQ---

---LAFQSISVNSIPPVFYNMLSQGLVSSTLFSFWLSRTPGANGGELSFGSIDNTKYTGD
ITYVPLTNETYWEFVMDDFAIDGQSAGFCGTTCHAICDSGTSLIAGPMADITALNEKLGA
VILNGEGVFSDCSVINTLPNVTITVAGREFVLTPKEYVLEVTEFGKTECLSGFMGIELNM
GNFWILGDVFISAYYTVFDFGNKQVGFATAIQG*iflinlylr*k*n*iieqy
Translated Amino Acid sequence (All Frames) 
Frame A:
lsfyvegm*sesfvwimnlwleffeskvrlntlkr*mrqi*vfhsay*lskrflcllmty
i*lspdlhkrk*nylf*lyf*lllf*lkl*qyh*tsiklqenleeefhkngqtdyllsml
vpqsqfqilkmlntmvplplvpqvkpsk*fsilvhptcgfhqrnvqslllhviyitnita
vpqahmsptelispsntvvvlcqvlslk---

---LAFQSISVNSIPPVFYNMLSQGLVSSTLFSFWLSRTPGANGGELSFGSIDNTKYTGD
ITYVPLTNETYWEFVMDDFAIDGQSAGFCGTTCHAICDSGTSLIAGPMADITALNEKLGA
VILNGEGVFSDCSVINTLPNVTITVAGREFVLTPKEYVLEVTEFGKTECLSGFMGIELNM
GNFWILGDVFISAYYTVFDFGNKQVGFATAIQG*iflinlylr*k*n*iieqy

Frame B:
fhftlrvcdpnhlcgs*iyg*sfsnlkfastl*kde*dkyryfivhinypsdfyas**pt
snchltyikeneityfnfifsyycfsssfnstiklpssfkri*kksstkmvkqiicsqcw
yhnpnfrf*rcsilwchyhwyprsslqssfrywfiqlvdsikemsnhcccm*ft*qi*qr
clkhicrqrn*fhhpir*wcyvrfclsr---

---llsnpslliqfhqsfttcyhkv*fhqhsspsgyqelqvptvvnshsvqsitpntlvt
lptsh*ptkpignslwmtllsmvnqlvsvvllvtqfaiqvhhsllvqwlillpsmkn*vl
ss*mvkvsslivalstpyqmlpspllvvnlf*lqkntf*kllsserlnv*vdlwvss*tw
eisgslvmfsslltilysilvinklvsqlpfkvkff*liyi*drnktk**nn

Frame C:
filr*gyviriicvdhefmarvfri*sspqhfkkmnetnigis*ciliiqaifmppndlh
livt*lt*KKMKLLILTLFLATIVLAQALTVPLNFHQASRESRRRVPQKWSNRLSALNAG
TTIPISDFEDAQYYGAITIGTPGQAFKVVFDTGSSNLWIPSKKCPITVVACDLHNKYNSG
ASSTYVANGTDFTIQYGSGAMSGFVSQ---

---cfpihlc*fnstsllqhvitrfsfintllllviknsrcqrw*tlirfnr*hqihw*h
ylrpinqrnllgiryg*lcyrwsiswflwyylsrnlrfryithcwsng*yycpq*kircc
hlkw*rcll*l*ryqhltkcyhhrcws*icfnskrirfrsy*vrkd*mfewiygyrvkhg
kfldpw*cfhlcllycirfw**tswfrnchsrlnffn*fifkieiklnnrti
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFB769 (VFB769Q) /CSM/VF/VFB7-C/VFB769Q.Seq.d/                       2526   0.0
VFM519 (VFM519Q) /CSM/VF/VFM5-A/VFM519Q.Seq.d/                       1326   0.0
VFL410 (VFL410Q) /CSM/VF/VFL4-A/VFL410Q.Seq.d/                       1326   0.0
VFL274 (VFL274Q) /CSM/VF/VFL2-D/VFL274Q.Seq.d/                       1326   0.0
VFK795 (VFK795Q) /CSM/VF/VFK7-D/VFK795Q.Seq.d/                       1326   0.0
VFI870 (VFI870Q) /CSM/VF/VFI8-C/VFI870Q.Seq.d/                       1326   0.0
VFG845 (VFG845Q) /CSM/VF/VFG8-B/VFG845Q.Seq.d/                       1326   0.0
VFG815 (VFG815Q) /CSM/VF/VFG8-A/VFG815Q.Seq.d/                       1326   0.0
VFF141 (VFF141Q) /CSM/VF/VFF1-B/VFF141Q.Seq.d/                       1326   0.0
VFD849 (VFD849Q) /CSM/VF/VFD8-C/VFD849Q.Seq.d/                       1326   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

Y16962|Y16962.1 Dictyostelium discoideum mRNA for cathepsin D.  1257  0.0  3
AJ243946|AJ243946.1 Dictyostelium discoideum ctsD gene for cathepsin D, exons 1 to 2.  1257  0.0  4
CB264579|CB264579.1 51-E015023-035-004-F13-T7R MPIZ-ADIS-035 Arabidopsis thaliana cDNA clone MPIZp2000F134Q 5-PRIME, mRNA sequence.    62  2e-05  1
AL765461|AL765461.1 Arabidopsis thaliana T-DNA flanking sequence GK-139E08-012875.    62  2e-05  1
AV567523|AV567523.1 Arabidopsis thaliana cDNA clone:SQL15g03F, 3' end.    62  2e-05  1
AL762930|AL762930.1 Arabidopsis thaliana T-DNA flanking sequence GK-030C11-011522.    62  2e-05  1
AF372974|AF372974.1 Arabidopsis thaliana AT4g04460/T26N6_7 mRNA, complete cds.    62  2e-05  1
CB264640|CB264640.1 48-E014661-035-002-P12-T7R MPIZ-ADIS-035 Arabidopsis thaliana cDNA clone MPIZp2000P122Q 5-PRIME, mRNA sequence.    62  2e-05  1
BE525959|BE525959.1 M64C07STM Arabidopsis developing seed Arabidopsis thaliana cDNA clone 600034526R1 5', mRNA sequence.    62  2e-05  1
CD825453|CD825453.1 BN25.060N03F011129 BN25 Brassica napus cDNA clone BN25060N03, mRNA sequence.    62  2e-05  1
dna update 2003. 9. 9
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q03168) RecName: Full=Lysosomal aspartic protease;          EC=...   319   2e-85
BT080419_1(BT080419|pid:none) Caligus clemensi clone ccle-evs-50...   315   2e-84
EF070454_1(EF070454|pid:none) Maconellicoccus hirsutus clone WHM...   313   1e-83
DQ010007_1(DQ010007|pid:none) Bombyx mori CathD mRNA, complete c...   310   6e-83
EF213114_1(EF213114|pid:none) Penaeus monodon cathepsin D mRNA, ...   307   6e-82
AB106552_1(AB106552|pid:none) Todarodes pacificus tpaD mRNA for ...   306   1e-81
FJ168036_1(FJ168036|pid:none) Fasciola hepatica cathepsin D-like...   304   4e-81
DQ909010_1(DQ909010|pid:none) Clonorchis sinensis aspartic prote...   303   7e-81
FJ654712_1(FJ654712|pid:none) Chrysomela tremulae aspartic prote...   297   6e-79
EF000001_1(EF000001|pid:none) Fasciola hepatica cathepsin D-like...   296   8e-79
protein update 2009. 6.17
PSORT 

 psg: 0.98  gvh: 0.77  alm: 0.42  top: 0.57  tms: 0.00  mit: 0.36  mip: 0.08
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: extracellular, including cell wall
	 16.0 %: mitochondrial
	 16.0 %: nuclear
	 12.0 %: vacuolar
	  8.0 %: cytoplasmic
	  4.0 %: cytoskeletal

>> prediction for VFB769 is exc
5' end seq. ID VFB769F
5' end seq. 
>VFB769F.Seq
CTTTCATTTTACGTTGAGGGTATGTGATCCGAATCATTTGTGTGGATCATGAATTTATGG
CTAGAGTTTTTCGAATCTAAAGTTCGCCTCAACACTTTAAAAAGATGAATGAGACAAATA
TAGGTATTTCATAGTGCATATTAATTATCCAAGCGATTTTTATGCCTCCTAATGACCTAC
ATCTAATTGTCACCTGACTTACATAAAAGAAAATGAAATTACTTATTTTAACTTTATTTT
TAGCTACTATTGTTTTAGCTCAAGCTTTAACAGTACCATTAAACTTCCATCAAGCTTCAA
GAGAATCTAGAAGAAGAGTTCCACAAAAATGGTCAAACAGATTATCTGCTCTCAATGCTG
GTACCACAATCCCAATTTCAGATTTTGAAGATGCTCAATACTATGGTGCCATTACCATTG
GTACCCCAGGTCAAGCCTTCAAAGTAGTTTTCGATACTGGTTCATCCAACTTGTGGATTC
CATCAAAGAAATGTCCAATCACTGTTGTTGCATGTGATTTACATAACAAATATAACAGCG
GTGCCTCAAGCACATATGTCGCCAACGGAACTGATTTCACCATCCAATACGGTAGTGGTG
CTATGTCAGGTTTTGTCTCTCAAGA----------
Length of 5' end seq. 625
3' end seq. ID VFB769Z
3' end seq. 
>VFB769Z.Seq
----------CTTGCTTTCCAATCCATCTCTGTTAATTCAATTCCACCAGTCTTTTACAA
CATGTTATCACAAGGTTTAGTTTCATCAACACTCTTCTCCTTCTGGTTATCAAGAACTCC
AGGTGCCAACGGTGGTGAACTCTCATTCGGTTCAATCGATAACACCAAATACACTGGTGA
CATTACCTACGTCCCATTAACCAACGAAACCTATTGGGAATTCGTTATGGATGACTTTGC
TATCGATGGTCAATCAGCTGGTTTCTGTGGTACTACTTGTCACGCAATTTGCGATTCAGG
TACATCACTCATTGCTGGTCCAATGGCTGATATTACTGCCCTCAATGAAAAATTAGGTGC
TGTCATCTTAAATGGTGAAGGTGTCTTCTCTGATTGTAGCGTTATCAACACCTTACCAAA
TGTTACCATCACCGTTGCTGGTCGTGAATTTGTTTTAACTCCAAAAGAATACGTTTTAGA
AGTTACTGAGTTCGGAAAGACTGAATGTTTGAGTGGATTTATGGGTATCGAGTTAAACAT
GGGAAATTTCTGGATCCTTGGTGATGTTTTCATCTCTGCTTACTATACTGTATTCGATTT
TGGTAATAAACAAGTTGGTTTCGCAACTGCCATTCAAGGTTAAATTTTTTTAATTAATTT
ATATTTAAGATAGAAATAAAACTAAATAATAGAACAATAT
Length of 3' end seq. 690
Connected seq. ID VFB769P
Connected seq. 
>VFB769P.Seq
CTTTCATTTTACGTTGAGGGTATGTGATCCGAATCATTTGTGTGGATCATGAATTTATGG
CTAGAGTTTTTCGAATCTAAAGTTCGCCTCAACACTTTAAAAAGATGAATGAGACAAATA
TAGGTATTTCATAGTGCATATTAATTATCCAAGCGATTTTTATGCCTCCTAATGACCTAC
ATCTAATTGTCACCTGACTTACATAAAAGAAAATGAAATTACTTATTTTAACTTTATTTT
TAGCTACTATTGTTTTAGCTCAAGCTTTAACAGTACCATTAAACTTCCATCAAGCTTCAA
GAGAATCTAGAAGAAGAGTTCCACAAAAATGGTCAAACAGATTATCTGCTCTCAATGCTG
GTACCACAATCCCAATTTCAGATTTTGAAGATGCTCAATACTATGGTGCCATTACCATTG
GTACCCCAGGTCAAGCCTTCAAAGTAGTTTTCGATACTGGTTCATCCAACTTGTGGATTC
CATCAAAGAAATGTCCAATCACTGTTGTTGCATGTGATTTACATAACAAATATAACAGCG
GTGCCTCAAGCACATATGTCGCCAACGGAACTGATTTCACCATCCAATACGGTAGTGGTG
CTATGTCAGGTTTTGTCTCTCAAGA----------CTTGCTTTCCAATCCATCTCTGTTA
ATTCAATTCCACCAGTCTTTTACAACATGTTATCACAAGGTTTAGTTTCATCAACACTCT
TCTCCTTCTGGTTATCAAGAACTCCAGGTGCCAACGGTGGTGAACTCTCATTCGGTTCAA
TCGATAACACCAAATACACTGGTGACATTACCTACGTCCCATTAACCAACGAAACCTATT
GGGAATTCGTTATGGATGACTTTGCTATCGATGGTCAATCAGCTGGTTTCTGTGGTACTA
CTTGTCACGCAATTTGCGATTCAGGTACATCACTCATTGCTGGTCCAATGGCTGATATTA
CTGCCCTCAATGAAAAATTAGGTGCTGTCATCTTAAATGGTGAAGGTGTCTTCTCTGATT
GTAGCGTTATCAACACCTTACCAAATGTTACCATCACCGTTGCTGGTCGTGAATTTGTTT
TAACTCCAAAAGAATACGTTTTAGAAGTTACTGAGTTCGGAAAGACTGAATGTTTGAGTG
GATTTATGGGTATCGAGTTAAACATGGGAAATTTCTGGATCCTTGGTGATGTTTTCATCT
CTGCTTACTATACTGTATTCGATTTTGGTAATAAACAAGTTGGTTTCGCAACTGCCATTC
AAGGTTAAATTTTTTTAATTAATTTATATTTAAGATAGAAATAAAACTAAATAATAGAAC
AATAT
Length of connected seq. 1315
Full length Seq ID -
Full length Seq. -
Length of full length seq. -