NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFB742
Library VF
(Link to library)
Clone ID VFB742
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U14919-1
Original site URL
Representative seq. ID VFB742P
(Link to Original site)
Representative DNA sequence 
>VFB742 (VFB742Q) /CSM/VF/VFB7-B/VFB742Q.Seq.d/
TATTTTATTTTACTCACACAAGTAATTATATAATATACATATACATATAAAATGGGTTCA
GTATCAGGTAATACACCATTAAAAGAAGTTGATAATGAAATCTTTGAATTAATGAATAGA
GAAAAAGATAGACAATTTAAAGGTTTAGAATTAATTGCCTCTGAAAATTTTACATCAAGA
GCAGTTATGGAAGCATTAGGATCACATTTTACAAATAAATATGCAGAAGGTTATCCAGGT
TCAAGATATTATGGTGGTACAGAAGTTGTTGATGAATTAGAAACTCTTTGTCAAAAGAGA
GCATTAAAAGCATTCCGTTTGGATGAGAGTAAATGGGGTGTTAATGTTCAACCATACAGT
GGTAGTCCAGCCAATTTCGCAGTTTACACAGCATTATTAAGACCACACGATCGTATAATG
GGTTTAGATTTACCATCAGGTGGTCATTTAACTCATGGTTATCAAACCGATAAGAAGAAG
ATTTCAGCATCATCAATCTTTTTCGAATCAATGCCATATCAAATTGGTGCTGATGGTTTA
ATCGATTATCAACGTTTAGAAGAGAATGCATTATTGTTCAAACCAAAATTXXXXXXXXXX
TNTCAAAGAANCNGACTCTCAAGAATTCAAAGAGTATGCCCTTNAAGTTAAAAAGAATGC
CGCTGCCATTGGTAATGCTCTCATGAACAAAGGTTATAAATTGGTTACAAATGGTACTGA
CAATCATTTAATTCTTTGGGATTTAAGACCAAAGGAACTCACTGGTAATAAATTTGAAAA
AGCTGCCGATATCGCTAATATCACCGTCAATAAAAATGCCGTCCATGGTGATACCAATGC
AATTTCACCAGGTGGTATTCGTATTGGTTCATCTGCTCTCACAAGTAGAGGTTTAAAGGA
AGCCGATTTTGAAAAGATTGCTGATTTCCTTGATAGAATCGTTTCAATCTCTCTTGAAAT
TCAAGGTCGTGTCGGTAAGAAATTGGTTGATTTTGTTGTTGAAATTAATAAATCAAAAGA
ATTATTAGATTTACGTAAAGAAGTTGAAGAATTTCTTCTGCAAAATTC
sequence update 2001. 6. 1
Translated Amino Acid sequence 
yfilltqvii*YTYTYKMGSVSGNTPLKEVDNEIFELMNREKDRQFKGLELIASENFTSR
AVMEALGSHFTNKYAEGYPGSRYYGGTEVVDELETLCQKRALKAFRLDESKWGVNVQPYS
GSPANFAVYTALLRPHDRIMGLDLPSGGHLTHGYQTDKKKISASSIFFESMPYQIGADGL
IDYQRLEENALLFKPK---

---XKEXDSQEFKEYALXVKKNAAAIGNALMNKGYKLVTNGTDNHLILWDLRPKELTGNK
FEKAADIANITVNKNAVHGDTNAISPGGIRIGSSALTSRGLKEADFEKIADFLDRIVSIS
LEIQGRVGKKLVDFVVEINKSKELLDLRKEVEEFLLQN
Translated Amino Acid sequence (All Frames) 
Frame A:
yfilltqvii*YTYTYKMGSVSGNTPLKEVDNEIFELMNREKDRQFKGLELIASENFTSR
AVMEALGSHFTNKYAEGYPGSRYYGGTEVVDELETLCQKRALKAFRLDESKWGVNVQPYS
GSPANFAVYTALLRPHDRIMGLDLPSGGHLTHGYQTDKKKISASSIFFESMPYQIGADGL
IDYQRLEENALLFKPK---

---xqrxxlsriqrvcpxs*kecrchw*csheqrl*igykwy*qsfnslgfktkgthw**
i*kscryr*yhrq*kcrpw*yqcnftrwysywficshk*rfkgsrf*kdc*fp**nrfnl
s*nsrscr*eig*fcc*n**ikriirft*rs*rissakf

Frame B:
ilfyshk*lynihihikwvqyqvihh*kklimksln**iekkidnlkv*n*lplkilhqe
qlwkh*dhilqinmqkviqvqdimvvqkllmn*klfvkreh*khsvwmrvngvlmfnhtv
vvqpisqftqhy*dhtiv*wv*iyhqvvi*lmvikpirrrfqhhqsfsnqchiklvlmv*
siinv*krmhycsnqn---

---XKEXDSQEFKEYALXVKKNAAAIGNALMNKGYKLVTNGTDNHLILWDLRPKELTGNK
FEKAADIANITVNKNAVHGDTNAISPGGIRIGSSALTSRGLKEADFEKIADFLDRIVSIS
LEIQGRVGKKLVDFVVEINKSKELLDLRKEVEEFLLQN

Frame C:
fyfthtsnyiiyiyi*ngfsir*ytikrs***nl*ine*rkr*ti*rfrincl*kfyiks
sygsiritfyk*icrrlsrfkilwwyrsc**irnslskesiksipfg*e*mgc*cstiqw
*ssqfrslhsiikttrsyngfrftirwsfnswlsnr*eedfsiinlfrinaisnwc*wfn
rlstfrreciivqtki---

---skxxtlknsksmpxklkrmplplvmls*tkvinwlqmvltii*ffgi*dqrnslvin
lkklpislispsikmpsmvipmqfhqvvfvlvhllsqvev*rkpilkrllisliesfqsl
lkfkvvsvrnwlilllklinqkny*iyvkklknffcki
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFB742 (VFB742Q) /CSM/VF/VFB7-B/VFB742Q.Seq.d/                       2034   0.0
VFN540 (VFN540Q) /CSM/VF/VFN5-B/VFN540Q.Seq.d/                       1170   0.0
VFM764 (VFM764Q) /CSM/VF/VFM7-C/VFM764Q.Seq.d/                       1170   0.0
VFK411 (VFK411Q) /CSM/VF/VFK4-A/VFK411Q.Seq.d/                       1170   0.0
SFI686 (SFI686Q) /CSM/SF/SFI6-D/SFI686Q.Seq.d/                       1170   0.0
AFO510 (AFO510Q) /CSM/AF/AFO5-A/AFO510Q.Seq.d/                       1170   0.0
AFN111 (AFN111Q) /CSM/AF/AFN1-A/AFN111Q.Seq.d/                       1168   0.0
AFE629 (AFE629Q) /CSM/AF/AFE6-B/AFE629Q.Seq.d/                       1168   0.0
SFF472 (SFF472Q) /CSM/SF/SFF4-C/SFF472Q.Seq.d/                       1162   0.0
CFF296 (CFF296Q) /CSM/CF/CFF2-D/CFF296Q.Seq.d/                       1162   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AF009966|AF009966.1 Candida albicans serine hydroxymethyl transferase II (SHMII) gene, complete cds.    58  1e-16  4
BI418085|BI418085.1 LjNEST24d6r Lotus japonicus nodule library 5 and 7 week-old Lotus japonicus cDNA 5', mRNA sequence.    60  4e-14  3
BP187516|BP187516.1 Dugesia japonica cDNA, clone: 00331_HN, expressed in the head.    52  2e-12  3
BQ489771|BQ489771.1 14-E9425-006-009-K03-T3 Sugar beet MPIZ-ADIS-006 Lambda Zap II library Beta vulgaris cDNA clone K-3-9, mRNA sequence.    50  2e-12  3
AL408772|AL408772.1 T7 end of clone AV0AA010F02 of library AV0AA from strain CBS 379 of Saccharomyces exiguus.    58  2e-11  3
AI960890|AI960890.1 sc92a10.y1 Gm-c1019 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1019-787 5' similar to TR:O04952 O04952 SERINE HYDROXYMETHYLTRANSFERASE ;, mRNA sequence.    52  2e-11  3
BG887269|BG887269.1 EST513120 cSTD Solanum tuberosum cDNA clone cSTD4I13 5' sequence, mRNA sequence.    46  2e-10  4
BQ866049|BQ866049.1 QGC6N09.yg.ab1 QG_ABCDI lettuce salinas Lactuca sativa cDNA clone QGC6N09, mRNA sequence.    78  3e-10  1
BI095900|BI095900.1 PIP2C_E6 Sugar Beet germination cDNA library Beta vulgaris cDNA 5' similar to glycine hydroxymethyl transferase, mRNA sequence.    50  5e-10  3
AL438035|AL438035.1 T3 end of clone BC0AA013A10 of library BC0AA from strain CBS 767 of Debaryomyces hansenii.    48  2e-08  3
dna update 2003. 9. 6
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AK302834_1(AK302834|pid:none) Homo sapiens cDNA FLJ60988 complet...   260   5e-68
AK223552_1(AK223552|pid:none) Homo sapiens mRNA for serine hydro...   260   5e-68
L23928_3(L23928|pid:none) Homo sapiens serine hydroxymethyltrans...   260   5e-68
BC022874_1(BC022874|pid:none) Homo sapiens serine hydroxymethylt...   260   5e-68
(P34896) RecName: Full=Serine hydroxymethyltransferase, cytosoli...   260   5e-68
(P07511) RecName: Full=Serine hydroxymethyltransferase, cytosoli...   259   9e-68
BC066496_1(BC066496|pid:none) Danio rerio serine hydroxymethyltr...   259   1e-67
AY850381_1(AY850381|pid:none) Danio rerio serine hydroxymethyltr...   259   1e-67
BT070546_1(BT070546|pid:none) Picea sitchensis clone WS02735_F19...   256   9e-67
BC055527_1(BC055527|pid:none) Danio rerio serine hydroxymethyltr...   256   1e-66
protein update 2009. 6.17
PSORT 

 psg: 0.81  gvh: 0.40  alm: 0.47  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 28.0 %: nuclear
	  8.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  4.0 %: plasma membrane
	  4.0 %: peroxisomal
	  4.0 %: endoplasmic reticulum

>> prediction for VFB742 is cyt
5' end seq. ID VFB742F
5' end seq. 
>VFB742F.Seq
TATTTTATTTTACTCACACAAGTAATTATATAATATACATATACATATAAAATGGGTTCA
GTATCAGGTAATACACCATTAAAAGAAGTTGATAATGAAATCTTTGAATTAATGAATAGA
GAAAAAGATAGACAATTTAAAGGTTTAGAATTAATTGCCTCTGAAAATTTTACATCAAGA
GCAGTTATGGAAGCATTAGGATCACATTTTACAAATAAATATGCAGAAGGTTATCCAGGT
TCAAGATATTATGGTGGTACAGAAGTTGTTGATGAATTAGAAACTCTTTGTCAAAAGAGA
GCATTAAAAGCATTCCGTTTGGATGAGAGTAAATGGGGTGTTAATGTTCAACCATACAGT
GGTAGTCCAGCCAATTTCGCAGTTTACACAGCATTATTAAGACCACACGATCGTATAATG
GGTTTAGATTTACCATCAGGTGGTCATTTAACTCATGGTTATCAAACCGATAAGAAGAAG
ATTTCAGCATCATCAATCTTTTTCGAATCAATGCCATATCAAATTGGTGCTGATGGTTTA
ATCGATTATCAACGTTTAGAAGAGAATGCATTATTGTTCAAACCAAAATT----------
Length of 5' end seq. 590
3' end seq. ID VFB742Z
3' end seq. 
>VFB742Z.Seq
----------TNTCAAAGAANCNGACTCTCAAGAATTCAAAGAGTATGCCCTTNAAGTTA
AAAAGAATGCCGCTGCCATTGGTAATGCTCTCATGAACAAAGGTTATAAATTGGTTACAA
ATGGTACTGACAATCATTTAATTCTTTGGGATTTAAGACCAAAGGAACTCACTGGTAATA
AATTTGAAAAAGCTGCCGATATCGCTAATATCACCGTCAATAAAAATGCCGTCCATGGTG
ATACCAATGCAATTTCACCAGGTGGTATTCGTATTGGTTCATCTGCTCTCACAAGTAGAG
GTTTAAAGGAAGCCGATTTTGAAAAGATTGCTGATTTCCTTGATAGAATCGTTTCAATCT
CTCTTGAAATTCAAGGTCGTGTCGGTAAGAAATTGGTTGATTTTGTTGTTGAAATTAATA
AATCAAAAGAATTATTAGATTTACGTAAAGAAGTTGAAGAATTTCTTCTGCAAAATTC
Length of 3' end seq. 468
Connected seq. ID VFB742P
Connected seq. 
>VFB742P.Seq
TATTTTATTTTACTCACACAAGTAATTATATAATATACATATACATATAAAATGGGTTCA
GTATCAGGTAATACACCATTAAAAGAAGTTGATAATGAAATCTTTGAATTAATGAATAGA
GAAAAAGATAGACAATTTAAAGGTTTAGAATTAATTGCCTCTGAAAATTTTACATCAAGA
GCAGTTATGGAAGCATTAGGATCACATTTTACAAATAAATATGCAGAAGGTTATCCAGGT
TCAAGATATTATGGTGGTACAGAAGTTGTTGATGAATTAGAAACTCTTTGTCAAAAGAGA
GCATTAAAAGCATTCCGTTTGGATGAGAGTAAATGGGGTGTTAATGTTCAACCATACAGT
GGTAGTCCAGCCAATTTCGCAGTTTACACAGCATTATTAAGACCACACGATCGTATAATG
GGTTTAGATTTACCATCAGGTGGTCATTTAACTCATGGTTATCAAACCGATAAGAAGAAG
ATTTCAGCATCATCAATCTTTTTCGAATCAATGCCATATCAAATTGGTGCTGATGGTTTA
ATCGATTATCAACGTTTAGAAGAGAATGCATTATTGTTCAAACCAAAATT----------
TNTCAAAGAANCNGACTCTCAAGAATTCAAAGAGTATGCCCTTNAAGTTAAAAAGAATGC
CGCTGCCATTGGTAATGCTCTCATGAACAAAGGTTATAAATTGGTTACAAATGGTACTGA
CAATCATTTAATTCTTTGGGATTTAAGACCAAAGGAACTCACTGGTAATAAATTTGAAAA
AGCTGCCGATATCGCTAATATCACCGTCAATAAAAATGCCGTCCATGGTGATACCAATGC
AATTTCACCAGGTGGTATTCGTATTGGTTCATCTGCTCTCACAAGTAGAGGTTTAAAGGA
AGCCGATTTTGAAAAGATTGCTGATTTCCTTGATAGAATCGTTTCAATCTCTCTTGAAAT
TCAAGGTCGTGTCGGTAAGAAATTGGTTGATTTTGTTGTTGAAATTAATAAATCAAAAGA
ATTATTAGATTTACGTAAAGAAGTTGAAGAATTTCTTCTGCAAAATTC
Length of connected seq. 1058
Full length Seq ID -
Full length Seq. -
Length of full length seq. -