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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFB732
Library VF
(Link to library)
Clone ID VFB732
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U02067-1|Contig-U09552-1
Original site URL
Representative seq. ID VFB732P
(Link to Original site)
Representative DNA sequence 
>VFB732 (VFB732Q) /CSM/VF/VFB7-B/VFB732Q.Seq.d/
CACTGTTGGCCTACGATTTTCCGCAATTTGAATCACTCTTGCACTTTTTTTTTATTATTA
TTATAATTATTATTGTTATAATTTGTATTATTATTAAAAAAAAAAXXXXXXXXXXCAGCT
GCTTATAAAACTGATTGTGTAGCTATGGCTTCTAATTATATTCCAGCAATTATTAAAATG
TTGGAAAATGATAATTCACCAGGTCAAGTTTGTCAAAAACTTAATCTTTGTCCAGCACCA
ACTCCAACTTCAACACCTTCAACAATCAAGATTGATGTGAATATTAATAAATGTGAAATT
TGTGAAACTGTAATTGGTTTTGCTGAAAAATATTTAGGTTCAGCATTAGATTCTCAAACT
GTAAAGACATTCCTCCAAAATGAATGTAACAAACTTCCAGGTGTTGTTGGTGATGTTTGT
AATGAAGTTGTAAATGAATATCTTGAATTTGCAGTCACTCAACTCGAAGCTAAATTTAAC
CCAACTGAAATTTGTCAATTATTTTCATTCTGTACTGCTGGATCTGAATCATCAGATGCA
TCTGAAATCAAAATTAATAACAACTCATATATTGATTGTCAAATTTGTGAAGTTTTAGCA
AATTTCGTAGTCTATGATAAGAATTATACAACTCATTTCGAAGATAAACTCAATAAATTA
TGTAGTTGTTTATCTCGTAGTTTAATTGAACCATGTCTTAACTTTGTTTCACAATANGGT
TGCTCCTCATGTGNATGTTGCCTGTTA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
LLAYDFPQFESLLHFFFIIIIIIIVIICIIIKKK---

---AAYKTDCVAMASNYIPAIIKMLENDNSPGQVCQKLNLCPAPTPTSTPSTIKIDVNIN
KCEICETVIGFAEKYLGSALDSQTVKTFLQNECNKLPGVVGDVCNEVVNEYLEFAVTQLE
AKFNPTEICQLFSFCTAGSESSDASEIKINNNSYIDCQICEVLANFVVYDKNYTTHFEDK
LNKLCSCLSRSLIEPCLNFVSQXGCSSCXCCLL
Translated Amino Acid sequence (All Frames) 
Frame A:
hcwptifrnlnhsctffllll*llll*fvlllkkk---

---qllikliv*lwlliifqqllkcwkmiihqvkfvknlifvqhqlqlqhlqqsrlm*il
invkfvkl*lvllkni*vqh*ilkl*rhsskmnvtnfqvllvmfvmkl*mnilnlqslns
klnltqlkfvnyfhsvlldlnhqmhlksklitthilivkfvkf*qis*smiriiqliski
nsinyvvvylvv*lnhvltlfhnxvaphvxvac

Frame B:
tvglrfsai*itlalffyyyynyycynlyyy*kk---

---scl*n*lcsygf*lyssny*nvgk**ftrsslskt*slsstnsnfntfnnqd*cey*
*m*nl*ncnwfc*kifrfsirfsnckdippk*m*qtsrccw*cl**sck*is*icshstr
s*i*pn*nlsiifilycwi*iirci*nqn**qliy*lsnl*sfskfrsl**elynsfrr*
tq*im*lfis*fn*tms*lcftixlllmxmlpv

Frame C:
LLAYDFPQFESLLHFFFIIIIIIIVIICIIIKKK---

---AAYKTDCVAMASNYIPAIIKMLENDNSPGQVCQKLNLCPAPTPTSTPSTIKIDVNIN
KCEICETVIGFAEKYLGSALDSQTVKTFLQNECNKLPGVVGDVCNEVVNEYLEFAVTQLE
AKFNPTEICQLFSFCTAGSESSDASEIKINNNSYIDCQICEVLANFVVYDKNYTTHFEDK
LNKLCSCLSRSLIEPCLNFVSQXGCSSCXCCLL
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFB732 (VFB732Q) /CSM/VF/VFB7-B/VFB732Q.Seq.d/                       1241   0.0
VFB595 (VFB595Q) /CSM/VF/VFB5-D/VFB595Q.Seq.d/                       1187   0.0
VFJ635 (VFJ635Q) /CSM/VF/VFJ6-B/VFJ635Q.Seq.d/                       1176   0.0
VFG662 (VFG662Q) /CSM/VF/VFG6-C/VFG662Q.Seq.d/                       1138   0.0
VFD617 (VFD617Q) /CSM/VF/VFD6-A/VFD617Q.Seq.d/                       1120   0.0
VFM205 (VFM205Q) /CSM/VF/VFM2-A/VFM205Q.Seq.d/                       1098   0.0
VFH555 (VFH555Q) /CSM/VF/VFH5-C/VFH555Q.Seq.d/                       1082   0.0
VFH580 (VFH580Q) /CSM/VF/VFH5-D/VFH580Q.Seq.d/                       1080   0.0
VFO888 (VFO888Q) /CSM/VF/VFO8-D/VFO888Q.Seq.d/                       1074   0.0
VFI241 (VFI241Q) /CSM/VF/VFI2-B/VFI241Q.Seq.d/                       1055   0.0
own update 2001.11.27
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

U66367|U66367.1 Dictyostelium discoideum SapA (sapA) mRNA, partial cds.  1195  0.0  1
AC115599|AC115599.2 Dictyostelium discoideum chromosome 2 map 4229098-4354721 strain AX4, complete sequence.    56  4e-05  4
BX072539|BX072539.2 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone CH211-286O24.    60  4e-05  1
AL844507|AL844507.1 Plasmodium falciparum chromosome 8.           60  4e-05  1
AL109815|AL109815.2 Plasmodium falciparum DNA *** SEQUENCING IN PROGRESS *** from  MAL13P10.    60  4e-05  7
BX001026|BX001026.4 Zebrafish DNA sequence from clone CH211-13B16 in linkage group 16.    60  4e-05  1
AY350617|AY350617.1 Apis mellifera complementary sex determiner (csd) mRNA, csd-b1_4 allele, complete cds.    60  4e-05  1
AC115684|AC115684.2 Dictyostelium discoideum chromosome 2 map 3108975-3191114 strain AX4, complete sequence.    60  4e-05  1
AC114261|AC114261.2 Dictyostelium discoideum chromosome 2 map 126059-159710 strain AX4, complete sequence.    60  4e-05  1
AC116551|AC116551.2 Dictyostelium discoideum chromosome 2 map complement(581327-427576) strain AX4, complete sequence.    60  4e-05  3
dna update 2003. 9. 6
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U66367_1(U66367|pid:none) Dictyostelium discoideum SapA (sapA) m...   385   e-105
AF196308_1(AF196308|pid:none) Naegleria fowleri naegleriapore A ...    71   4e-11
BC054988_1(BC054988|pid:none) Xenopus laevis hypothetical protei...    70   9e-11
BC080997_1(BC080997|pid:none) Xenopus laevis MGC80725 protein, m...    65   2e-09
AF108656_1(AF108656|pid:none) Gallus gallus prosaposin mRNA, com...    62   1e-08
(O13035) RecName: Full=Proactivator polypeptide; Contains:   Rec...    62   1e-08
DQ062781_1(DQ062781|pid:none) Drosophila simulans saposin-relate...    62   1e-08
CR456746_1(CR456746|pid:none) Homo sapiens full open reading fra...    62   2e-08
AK223290_1(AK223290|pid:none) Homo sapiens mRNA for prosaposin (...    62   2e-08
(Q6IBQ6) RecName: Full=Proactivator polypeptide; Contains:   Rec...    62   2e-08
protein update 2009. 6.17
PSORT 

 psg: 0.75  gvh: 0.48  alm: -0.03  top: 0.77  tms: 0.07  mit: 0.20  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 1.00  yqr: 1.00  tyr: 0.00  leu: 0.02  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 1.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 44.0 %: nuclear
	 28.0 %: cytoplasmic
	 12.0 %: mitochondrial
	  8.0 %: Golgi
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for VFB732 is nuc
5' end seq. ID VFB732F
5' end seq. 
>VFB732F.Seq
CACTGTTGGCCTACGATTTTCCGCAATTTGAATCACTCTTGCACTTTTTTTTTATTATTA
TTATAATTATTATTGTTATAATTTGTATTATTATTAAAAAAAAAA----------
Length of 5' end seq. 105
3' end seq. ID VFB732Z
3' end seq. 
>VFB732Z.Seq
----------CAGCTGCTTATAAAACTGATTGTGTAGCTATGGCTTCTAATTATATTCCA
GCAATTATTAAAATGTTGGAAAATGATAATTCACCAGGTCAAGTTTGTCAAAAACTTAAT
CTTTGTCCAGCACCAACTCCAACTTCAACACCTTCAACAATCAAGATTGATGTGAATATT
AATAAATGTGAAATTTGTGAAACTGTAATTGGTTTTGCTGAAAAATATTTAGGTTCAGCA
TTAGATTCTCAAACTGTAAAGACATTCCTCCAAAATGAATGTAACAAACTTCCAGGTGTT
GTTGGTGATGTTTGTAATGAAGTTGTAAATGAATATCTTGAATTTGCAGTCACTCAACTC
GAAGCTAAATTTAACCCAACTGAAATTTGTCAATTATTTTCATTCTGTACTGCTGGATCT
GAATCATCAGATGCATCTGAAATCAAAATTAATAACAACTCATATATTGATTGTCAAATT
TGTGAAGTTTTAGCAAATTTCGTAGTCTATGATAAGAATTATACAACTCATTTCGAAGAT
AAACTCAATAAATTATGTAGTTGTTTATCTCGTAGTTTAATTGAACCATGTCTTAACTTT
GTTTCACAATANGGTTGCTCCTCATGTGNATGTTGCCTGTTA
Length of 3' end seq. 632
Connected seq. ID VFB732P
Connected seq. 
>VFB732P.Seq
CACTGTTGGCCTACGATTTTCCGCAATTTGAATCACTCTTGCACTTTTTTTTTATTATTA
TTATAATTATTATTGTTATAATTTGTATTATTATTAAAAAAAAAA----------CAGCT
GCTTATAAAACTGATTGTGTAGCTATGGCTTCTAATTATATTCCAGCAATTATTAAAATG
TTGGAAAATGATAATTCACCAGGTCAAGTTTGTCAAAAACTTAATCTTTGTCCAGCACCA
ACTCCAACTTCAACACCTTCAACAATCAAGATTGATGTGAATATTAATAAATGTGAAATT
TGTGAAACTGTAATTGGTTTTGCTGAAAAATATTTAGGTTCAGCATTAGATTCTCAAACT
GTAAAGACATTCCTCCAAAATGAATGTAACAAACTTCCAGGTGTTGTTGGTGATGTTTGT
AATGAAGTTGTAAATGAATATCTTGAATTTGCAGTCACTCAACTCGAAGCTAAATTTAAC
CCAACTGAAATTTGTCAATTATTTTCATTCTGTACTGCTGGATCTGAATCATCAGATGCA
TCTGAAATCAAAATTAATAACAACTCATATATTGATTGTCAAATTTGTGAAGTTTTAGCA
AATTTCGTAGTCTATGATAAGAATTATACAACTCATTTCGAAGATAAACTCAATAAATTA
TGTAGTTGTTTATCTCGTAGTTTAATTGAACCATGTCTTAACTTTGTTTCACAATANGGT
TGCTCCTCATGTGNATGTTGCCTGTTA
Length of connected seq. 737
Full length Seq ID -
Full length Seq. -
Length of full length seq. -