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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFM194
Library AF
(Link to library)
Clone ID AFM194
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID AFM194P
(Link to Original site)
Representative DNA sequence 
>AFM194 (AFM194Q) /CSM/AF/AFM1-D/AFM194Q.Seq.d/
ATTTTATAATCACTGTATAAACAAAAATTTATATATATATATATAGAATTTAAATAAAGA
TATGTTCAAACAAACATTTAAAGTAAATATTATACCGACCCAACCATTTGAAGGTCAAAA
ACCAGGTACATCTGGTTTAAGAAAAAAAGTAACTGAAGTAATGAAGACAAATTACCTTGG
TAACTTTGTTCAATCAATTTTCAATGCTTTACCAGAAGATAAATTAAAAGGATCAACTAT
TGTTGTTGGTGGTGATGGTCGTTACTACAATAATGATGCTATTCAATTAATTTTTCAAAT
TGCAGCAGCCAATGGAGTTGGAAAGATTTTAGTAGGAAGATATGGTTTACTTTCAACACC
AGCAATCTCTGCAATCGTTCGTGCTCGTTCAGCATTGGGTGCAATCATTTTAACAGCATC
ACATAATCCAGGTGGTCCAAATGGTGATTTTGGTATTAAATATAATATGTCCAATGGTGG
TCCAGCTCCAGAATCAATCACCAATGCAATCTATAAACATACCACATCAATCACACAAAT
TAAGACTACTAGAAATGTAAGTGTCGACAATTTGGGTTTGCTTAAAACTTATGAATGGAA
TGATGGTGAATTCXXXXXXXXXXTTTGAAGTACCAACAGGTTGGAAATTCTTTGGTAACC
TTATGGATGCTGGTACCCTCTCAATTTGTGGTGAGGAATCATTTGGTACTGGTTCTGATC
ATATTCGTGAAAAAGATGGTATTTGGGCTATCATTTGTTGGTTACAAATTTTAACTCATC
ATAATCAATCAACCAATGATAAGAACTTTGTTTCCATTGAAGAGATTGTCAAACAACATT
GGGCAAAATATGGTAGAAACTATTATTCTCGTTACGATTATGAAGAAATTGACACTGCTC
CAGCCGAAGCAATGATGAAACATGTTTCTCAACAAATTGAATCCAAACAATTGATTGGTA
AGAAATTCACTGGTATCTCTGATTCTTTGGAATATGAAATTGCATCTTGTGATGATTTTG
AATATAAAGATCCAATTGATAGTTCAGTTTCCTCTCATCAAGGTCTTCGTATCATTTTCA
CTGATGGCTCAAGAATTATCTATCGTTTAAGTGGTACTGGTTCAACTGGTGCCACAGTTC
GTGTTTATTTCGATAAATATGAAACTCAACCTACTCAATTAAATAATGATGTTCAAACTC
ATCTTAAATCTTTAATTCATATTGCACTTGTGAAATTTCAAAATTAAATCATTATACTGG
TAGAAATNAACCAAATGTAATTACATAAATAAATAAAATTTAATAACAAATAATAA
sequence update 2009. 4. 3
Translated Amino Acid sequence 
fynhcinknlyiyi*NLNKDMFKQTFKVNIIPTQPFEGQKPGTSGLRKKVTEVMKTNYLG
NFVQSIFNALPEDKLKGSTIVVGGDGRYYNNDAIQLIFQIAAANGVGKILVGRYGLLSTP
AISAIVRARSALGAIILTASHNPGGPNGDFGIKYNMSNGGPAPESITNAIYKHTTSITQI
KTTRNVSVDNLGLLKTYEWNDGEF---

---FEVPTGWKFFGNLMDAGTLSICGEESFGTGSDHIREKDGIWAIICWLQILTHHNQST
NDKNFVSIEEIVKQHWAKYGRNYYSRYDYEEIDTAPAEAMMKHVSQQIESKQLIGKKFTG
ISDSLEYEIASCDDFEYKDPIDSSVSSHQGLRIIFTDGSRIIYRLSGTGSTGATVRVYFD
KYETQPTQLNNDVQTHLKSLIHIALVKFQN*iiilvexnqm*lhk*ikfnnk**
Translated Amino Acid sequence (All Frames) 
Frame A:
il*slykqkfiyiyiefk*ryvqtni*skyytdpti*rsktryiwfkkksn*snedklpw
*lcsinfqcftrr*ikrinyccww*wsllq**cysinfsncssqwswkdfsrkiwftfnt
snlcnrscsfsigcnhfnsit*srwskw*fwy*i*yvqwwsssrinhqcnl*tyhinhtn
*dy*kckcrqfgfa*nl*me*w*i---

---FEVPTGWKFFGNLMDAGTLSICGEESFGTGSDHIREKDGIWAIICWLQILTHHNQST
NDKNFVSIEEIVKQHWAKYGRNYYSRYDYEEIDTAPAEAMMKHVSQQIESKQLIGKKFTG
ISDSLEYEIASCDDFEYKDPIDSSVSSHQGLRIIFTDGSRIIYRLSGTGSTGATVRVYFD
KYETQPTQLNNDVQTHLKSLIHIALVKFQN*iiilvexnqm*lhk*ikfnnk**

Frame B:
fynhcinknlyiyi*NLNKDMFKQTFKVNIIPTQPFEGQKPGTSGLRKKVTEVMKTNYLG
NFVQSIFNALPEDKLKGSTIVVGGDGRYYNNDAIQLIFQIAAANGVGKILVGRYGLLSTP
AISAIVRARSALGAIILTASHNPGGPNGDFGIKYNMSNGGPAPESITNAIYKHTTSITQI
KTTRNVSVDNLGLLKTYEWNDGEF---

---lkyqqvgnslvtlwmlvpsqfvvrnhlvlvliifvkkmvfglsfvgykf*liiinqp
mirtlfplkrlsnnigqnmvetiilvtimkkltllqpkq**nmflnklnpnn*lvrnslv
slilwnmklhlvmilnikiqlivqfplikvfvsfslmaqelsiv*vvlvqlvpqfvfisi
nmklnlln*immfklilnl*filhl*nfkikslyw*kxtkcnyink*nlitnn

Frame C:
fiitv*tkiyiyiyri*ikicsnkhlk*ilyrpnhlkvknqvhlv*ekk*lk**rqitlv
tlfnqfsmlyqkin*kdqllllvvmvvttimmlfn*ffklqqpmelerf**edmvyfqhq
qslqsfvlvqhwvqsf*qhhiiqvvqmvilvlniicpmvvqlqnqspmqsiniphqshkl
rllem*vstiwvclklmngmmvn---

---*stnrleilw*pygcwyplnlw*giiwywf*sys*krwylgyhllvtnfnss*sinq
**elcfh*rdcqttlgkiw*kllfslrl*rn*hcssrsndetcfstn*iqtidw*eihwy
l*ffgi*ncil**f*i*rsn**fsflssrssyhfh*wlknylsfkwywfnwchssclfr*
i*nstysik**csnss*ifnsyctceisklnhytgrnxpnvit*inki**qii
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFM194 (AFM194Q) /CSM/AF/AFM1-D/AFM194Q.Seq.d/                       2323   0.0
VHK768 (VHK768Q) /CSM/VH/VHK7-C/VHK768Q.Seq.d/                       2288   0.0
VHD683 (VHD683Q) /CSM/VH/VHD6-D/VHD683Q.Seq.d/                       1265   0.0
AFO149 (AFO149Q) /CSM/AF/AFO1-C/AFO149Q.Seq.d/                       1265   0.0
AFI424 (AFI424Q) /CSM/AF/AFI4-A/AFI424Q.Seq.d/                       1265   0.0
VFL815 (VFL815Q) /CSM/VF/VFL8-A/VFL815Q.Seq.d/                       1253   0.0
VHL451 (VHL451Q) /CSM/VH/VHL4-C/VHL451Q.Seq.d/                       1247   0.0
CHB413 (CHB413Q) /CSM/CH/CHB4-A/CHB413Q.Seq.d/                       1239   0.0
AFM220 (AFM220Q) /CSM/AF/AFM2-A/AFM220Q.Seq.d/                       1215   0.0
AFF233 (AFF233Q) /CSM/AF/AFF2-B/AFF233Q.Seq.d/                       1215   0.0
own update 2009. 4. 3
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ347029) Dictyostelium discoideum cDNA clone:dda25k23, 3' ...  1281   0.0    1
(BJ434544) Dictyostelium discoideum cDNA clone:ddv24n04, 3' ...  1251   0.0    1
(U61984) Dictyostelium discoideum phosphoglucomutase A (pgmA...  1205   0.0    2
(BJ438893) Dictyostelium discoideum cDNA clone:ddv38e22, 3' ...  1205   0.0    2
(BJ377574) Dictyostelium discoideum cDNA clone:ddc24j04, 3' ...  1205   0.0    2
(BJ345910) Dictyostelium discoideum cDNA clone:dda29a13, 3' ...  1205   0.0    2
(BJ343799) Dictyostelium discoideum cDNA clone:dda17p06, 3' ...  1205   0.0    2
(BJ443329) Dictyostelium discoideum cDNA clone:ddv52h17, 3' ...  1203   0.0    2
(BJ443626) Dictyostelium discoideum cDNA clone:ddv53f14, 3' ...  1191   0.0    2
(BJ346798) Dictyostelium discoideum cDNA clone:dda25g06, 3' ...  1156   0.0    2
dna update 2009. 4. 9
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q23919) RecName: Full=Phosphoglucomutase-1;          Short=PGM ...   420   e-121
AE017356_43(AE017356|pid:none) Cryptococcus neoformans var. neof...   249   4e-66
BT043631_1(BT043631|pid:none) Salmo salar clone HM5_0587 phospho...   251   3e-65
AY059926_1(AY059926|pid:none) Arabidopsis thaliana phosphoglucom...   247   7e-65
(Q9SGC1) RecName: Full=Probable phosphoglucomutase, cytoplasmic ...   246   1e-64
(Q9M4G4) RecName: Full=Phosphoglucomutase, cytoplasmic;         ...   243   3e-63
AK064893_1(AK064893|pid:none) Oryza sativa Japonica Group cDNA c...   241   5e-63
AX030362_1(AX030362|pid:none) Sequence 3 from Patent EP1001029.       239   1e-62
(P93262) RecName: Full=Phosphoglucomutase, cytoplasmic;         ...   241   2e-62
(P93805) RecName: Full=Phosphoglucomutase, cytoplasmic 2;       ...   238   2e-62
protein update 2009. 4. 5
PSORT 

 psg: 0.00  gvh: 0.00  alm: 0.00  top: 0.00  tms: 0.00  mit: 0.00  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	100.0 %: 

>> prediction for AFM194 is

5' end seq. ID AFM194F
5' end seq. 
>AFM194F.Seq
ATTTTATAATCACTGTATAAACAAAAATTTATATATATATATATAGAATTTAAATAAAGA
TATGTTCAAACAAACATTTAAAGTAAATATTATACCGACCCAACCATTTGAAGGTCAAAA
ACCAGGTACATCTGGTTTAAGAAAAAAAGTAACTGAAGTAATGAAGACAAATTACCTTGG
TAACTTTGTTCAATCAATTTTCAATGCTTTACCAGAAGATAAATTAAAAGGATCAACTAT
TGTTGTTGGTGGTGATGGTCGTTACTACAATAATGATGCTATTCAATTAATTTTTCAAAT
TGCAGCAGCCAATGGAGTTGGAAAGATTTTAGTAGGAAGATATGGTTTACTTTCAACACC
AGCAATCTCTGCAATCGTTCGTGCTCGTTCAGCATTGGGTGCAATCATTTTAACAGCATC
ACATAATCCAGGTGGTCCAAATGGTGATTTTGGTATTAAATATAATATGTCCAATGGTGG
TCCAGCTCCAGAATCAATCACCAATGCAATCTATAAACATACCACATCAATCACACAAAT
TAAGACTACTAGAAATGTAAGTGTCGACAATTTGGGTTTGCTTAAAACTTATGAATGGAA
TGATGGTGAATTCXXXXXXXXXX
Length of 5' end seq. 623
3' end seq. ID AFM194Z
3' end seq. 
>AFM194Z.Seq
XXXXXXXXXXTTTGAAGTACCAACAGGTTGGAAATTCTTTGGTAACCTTATGGATGCTGG
TACCCTCTCAATTTGTGGTGAGGAATCATTTGGTACTGGTTCTGATCATATTCGTGAAAA
AGATGGTATTTGGGCTATCATTTGTTGGTTACAAATTTTAACTCATCATAATCAATCAAC
CAATGATAAGAACTTTGTTTCCATTGAAGAGATTGTCAAACAACATTGGGCAAAATATGG
TAGAAACTATTATTCTCGTTACGATTATGAAGAAATTGACACTGCTCCAGCCGAAGCAAT
GATGAAACATGTTTCTCAACAAATTGAATCCAAACAATTGATTGGTAAGAAATTCACTGG
TATCTCTGATTCTTTGGAATATGAAATTGCATCTTGTGATGATTTTGAATATAAAGATCC
AATTGATAGTTCAGTTTCCTCTCATCAAGGTCTTCGTATCATTTTCACTGATGGCTCAAG
AATTATCTATCGTTTAAGTGGTACTGGTTCAACTGGTGCCACAGTTCGTGTTTATTTCGA
TAAATATGAAACTCAACCTACTCAATTAAATAATGATGTTCAAACTCATCTTAAATCTTT
AATTCATATTGCACTTGTGAAATTTCAAAATTAAATCATTATACTGGTAGAAATNAACCA
AATGTAATTACATAAATAAATAAAATTTAATAACAAATAATAA
Length of 3' end seq. 703
Connected seq. ID AFM194P
Connected seq. 
>AFM194P.Seq
ATTTTATAATCACTGTATAAACAAAAATTTATATATATATATATAGAATTTAAATAAAGA
TATGTTCAAACAAACATTTAAAGTAAATATTATACCGACCCAACCATTTGAAGGTCAAAA
ACCAGGTACATCTGGTTTAAGAAAAAAAGTAACTGAAGTAATGAAGACAAATTACCTTGG
TAACTTTGTTCAATCAATTTTCAATGCTTTACCAGAAGATAAATTAAAAGGATCAACTAT
TGTTGTTGGTGGTGATGGTCGTTACTACAATAATGATGCTATTCAATTAATTTTTCAAAT
TGCAGCAGCCAATGGAGTTGGAAAGATTTTAGTAGGAAGATATGGTTTACTTTCAACACC
AGCAATCTCTGCAATCGTTCGTGCTCGTTCAGCATTGGGTGCAATCATTTTAACAGCATC
ACATAATCCAGGTGGTCCAAATGGTGATTTTGGTATTAAATATAATATGTCCAATGGTGG
TCCAGCTCCAGAATCAATCACCAATGCAATCTATAAACATACCACATCAATCACACAAAT
TAAGACTACTAGAAATGTAAGTGTCGACAATTTGGGTTTGCTTAAAACTTATGAATGGAA
TGATGGTGAATTC----------TTTGAAGTACCAACAGGTTGGAAATTCTTTGGTAACC
TTATGGATGCTGGTACCCTCTCAATTTGTGGTGAGGAATCATTTGGTACTGGTTCTGATC
ATATTCGTGAAAAAGATGGTATTTGGGCTATCATTTGTTGGTTACAAATTTTAACTCATC
ATAATCAATCAACCAATGATAAGAACTTTGTTTCCATTGAAGAGATTGTCAAACAACATT
GGGCAAAATATGGTAGAAACTATTATTCTCGTTACGATTATGAAGAAATTGACACTGCTC
CAGCCGAAGCAATGATGAAACATGTTTCTCAACAAATTGAATCCAAACAATTGATTGGTA
AGAAATTCACTGGTATCTCTGATTCTTTGGAATATGAAATTGCATCTTGTGATGATTTTG
AATATAAAGATCCAATTGATAGTTCAGTTTCCTCTCATCAAGGTCTTCGTATCATTTTCA
CTGATGGCTCAAGAATTATCTATCGTTTAAGTGGTACTGGTTCAACTGGTGCCACAGTTC
GTGTTTATTTCGATAAATATGAAACTCAACCTACTCAATTAAATAATGATGTTCAAACTC
ATCTTAAATCTTTAATTCATATTGCACTTGTGAAATTTCAAAATTAAATCATTATACTGG
TAGAAATNAACCAAATGTAATTACATAAATAAATAAAATTTAATAACAAATAATAA
Length of connected seq. 1306
Full length Seq ID -
Full length Seq. -
Length of full length seq. -