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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFB720
Library VF
(Link to library)
Clone ID VFB720
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16603-1
Original site URL
Representative seq. ID VFB720P
(Link to Original site)
Representative DNA sequence 
>VFB720 (VFB720Q) /CSM/VF/VFB7-A/VFB720Q.Seq.d/
TTTAAAAAATCAATTAAAATTTAGACATTTTGATGATAGTTTAGATTATGATGCAATCAA
TTATCAATGGTTCACTCAAAGTGTTGATCATTTTAATCCAGCAAACCCAACAACTTTCCA
ACAAAGATATTTAATTAATGACCAATATTGGGATGGTACAGGTCCAGTTTTTATCATGAT
TAATGGTGAAGGTCCAATGGATATTAATACTGTAACCCAACTTCAATTTGTTGTTTGGGC
TAAACAAGTTAGTGCACTTGTTGTTTCTCTTGAACATCGTTATTATGGTGCTAGTTTTGT
AACTGAAGACCTTTCACTTGAAAATTTACAATGGTTAAATTCAGCACAAGCATTAGCAGA
TAATGCAGTTTTTAGAAATTTTGTAGCACAACAATATAATGTACCAAAAGAATCAAAATG
GATTTCATTTGGTGGTAGTTATTCAGGAGCATTAACTTCATGGTTTAGAATTAAATATCC
ACATTTAGTTGATGCCACCATTGCATCAAGTGCACCAGTTAXXXXXXXXXXTGTGTTGAA
TTTGGTTATTATCAATCAAGTGATGCTCCATCTGCAAATCAACCATTTGGTAATTTATTC
CCATTCCAACCATATCAAATTCAACAATGTGCCGATAGTTTTGGAATTCNAAATATGTAT
CCAAACGTTAATTGGACAATCACTGAATATGGTGGTATTAATCCAGAACCATCCTCTGTT
GATAATACCTTATATGTAAATGGTTCAAATGATGAATGGCACAATCTTGCAATCTTACCA
GGTAATGCAAATGCTAAAAATACTTTATACATTATTGGTACNAGTCNTTGTGCTGATATG
ATGATTCCAACTTCAGTATCACCTTCCAATTTCGGGNACAAGCA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
LKNQLKFRHFDDSLDYDAINYQWFTQSVDHFNPANPTTFQQRYLINDQYWDGTGPVFIMI
NGEGPMDINTVTQLQFVVWAKQVSALVVSLEHRYYGASFVTEDLSLENLQWLNSAQALAD
NAVFRNFVAQQYNVPKESKWISFGGSYSGALTSWFRIKYPHLVDATIASSAPV---

---CVEFGYYQSSDAPSANQPFGNLFPFQPYQIQQCADSFGIXNMYPNVNWTITEYGGIN
PEPSSVDNTLYVNGSNDEWHNLAILPGNANAKNTLYIIGTSXCADMMIPTSVSPSNFGXK
Translated Amino Acid sequence (All Frames) 
Frame A:
fkksiki*tf***frl*cnqlsmvhskc*sf*sskpnnfptkifn**pilgwyrssfyhd
*w*rsngy*ycnptsicclg*ts*ctccfs*tsllwc*fcn*rpft*kftmvkfstsisr
*csf*kfcstti*ctkrikmdfiww*lfrsinfmv*n*istfs*chhcikcts---

---CVEFGYYQSSDAPSANQPFGNLFPFQPYQIQQCADSFGIXNMYPNVNWTITEYGGIN
PEPSSVDNTLYVNGSNDEWHNLAILPGNANAKNTLYIIGTSXCADMMIPTSVSPSNFGXK

Frame B:
LKNQLKFRHFDDSLDYDAINYQWFTQSVDHFNPANPTTFQQRYLINDQYWDGTGPVFIMI
NGEGPMDINTVTQLQFVVWAKQVSALVVSLEHRYYGASFVTEDLSLENLQWLNSAQALAD
NAVFRNFVAQQYNVPKESKWISFGGSYSGALTSWFRIKYPHLVDATIASSAPV---

---vlnlviinqvmlhlqinhlviyshsnhikfnnvpivlefxiciqtligqslnmvvli
qnhplliipym*mvqmmngtilqsyqvmqmlkilytllvxvxvli**fqlqyhlpisgts

Frame C:
*kin*nldilmiv*immqsiingslkvliiliqqtqqlsnkdi*lmtnigmvqvqfls*l
mvkvqwilil*pnfnllfglnklvhllfllnivimvlvl*lktfhlkiyng*iqhkh*qi
mqfleil*hnnimyqknqngfhlvvviqeh*lhglelnihi*lmpplhqvhql---

---c*iwllsik*csickstiw*fipiptisnstmcr*fwnskyvskr*ldnh*iwwy*s
rtilc**ylickwfk**maqscnltr*ckc*kyfihywyxslc*yddsnfsitfqfrxqa
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFB720 (VFB720Q) /CSM/VF/VFB7-A/VFB720Q.Seq.d/                       1669   0.0
VSJ823 (VSJ823Q) /CSM/VS/VSJ8-A/VSJ823Q.Seq.d/                       1033   0.0
VFM601 (VFM601Q) /CSM/VF/VFM6-A/VFM601Q.Seq.d/                       1033   0.0
VFK228 (VFK228Q) /CSM/VF/VFK2-B/VFK228Q.Seq.d/                       1033   0.0
VFJ344 (VFJ344Q) /CSM/VF/VFJ3-B/VFJ344Q.Seq.d/                       1033   0.0
VFJ245 (VFJ245Q) /CSM/VF/VFJ2-B/VFJ245Q.Seq.d/                       1033   0.0
VFC861 (VFC861Q) /CSM/VF/VFC8-C/VFC861Q.Seq.d/                       1033   0.0
VFA660 (VFA660Q) /CSM/VF/VFA6-C/VFA660Q.Seq.d/                       1033   0.0
VFA596 (VFA596Q) /CSM/VF/VFA5-D/VFA596Q.Seq.d/                       1033   0.0
VFA473 (VFA473Q) /CSM/VF/VFA4-D/VFA473Q.Seq.d/                       1033   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

BI920191|BI920191.1 EST540126 potato microtubers, in vitro-grown Solanum tuberosum cDNA clone cSTE24M6 5' end, mRNA sequence.    44  2e-05  2
BI178421|BI178421.1 EST519366 cSTE Solanum tuberosum cDNA clone cSTE13N22 5' sequence, mRNA sequence.    40  0.16  2
CB097763|CB097763.1 ku44g10.y1 Strongyloides ratti PA female naive pAMP1 v1 Strongyloides ratti cDNA 5' similar to SW:YM67_CAEEL P34528 HYPOTHETICAL 65.3 KD PROTEIN K12H4.7 IN CHROMOSOME III. [1] ;, mRNA sequence.    48  0.19  1
CB097614|CB097614.1 ku42h12.y1 Strongyloides ratti PA female naive pAMP1 v1 Strongyloides ratti cDNA 5' similar to SW:YM67_CAEEL P34528 HYPOTHETICAL 65.3 KD PROTEIN K12H4.7 IN CHROMOSOME III. [1] ;, mRNA sequence.    48  0.19  1
AP004475|AP004475.1 Lotus japonicus genomic DNA, chromosome 1, clone:LjT01I13, TM0010.    44  0.39  2
AC108206|AC108206.3 Homo sapiens BAC clone RP11-203B7 from 4, complete sequence.    34  0.48  7
BM520804|BM520804.1 sal30g03.y1 Gm-c1059 Glycine max cDNA clone SOYBEAN CLONE ID: Gm-c1059-3773 5', mRNA sequence.    46  0.75  1
AI900122|AI900122.1 sc01b07.y1 Gm-c1012 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1012-878 5', mRNA sequence.    46  0.75  1
AW705505|AW705505.1 sk49h12.y1 Gm-c1019 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1019-5952 5', mRNA sequence.    46  0.75  1
AI441512|AI441512.1 sa87c11.y1 Gm-c1004 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1004-6285 5', mRNA sequence.    46  0.75  1
dna update 2003. 9. 6
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BC133755_1(BC133755|pid:none) Xenopus laevis hypothetical LOC495...   160   5e-38
BC108759_1(BC108759|pid:none) Xenopus laevis hypothetical protei...   160   5e-38
BC161646_1(BC161646|pid:none) Xenopus tropicalis hypothetical pr...   154   5e-36
BT080886_1(BT080886|pid:none) Caligus clemensi clone ccle-evs-50...   147   6e-34
AE014296_1236(AE014296|pid:none) Drosophila melanogaster chromos...   137   4e-31
S44851(S44851)K12H4.7 protein - Caenorhabditis elegans                136   1e-30
L14331_6(L14331|pid:none) Caenorhabditis elegans cosmid K12H4, c...   136   1e-30
(P34528) RecName: Full=Putative serine protease K12H4.7;        ...   136   1e-30
FB848274_1(FB848274|pid:none) Sequence 67547 from Patent WO20080...   135   2e-30
T16490(T16490)hypothetical protein F56F10.1 - Caenorhabditis ele...   135   2e-30
protein update 2009. 6.17
PSORT 

 psg: 0.55  gvh: 0.30  alm: 0.37  top: 0.53  tms: 0.00  mit: 0.23  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 36.0 %: cytoplasmic
	  8.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for VFB720 is nuc
5' end seq. ID VFB720F
5' end seq. 
>VFB720F.Seq
TTTAAAAAATCAATTAAAATTTAGACATTTTGATGATAGTTTAGATTATGATGCAATCAA
TTATCAATGGTTCACTCAAAGTGTTGATCATTTTAATCCAGCAAACCCAACAACTTTCCA
ACAAAGATATTTAATTAATGACCAATATTGGGATGGTACAGGTCCAGTTTTTATCATGAT
TAATGGTGAAGGTCCAATGGATATTAATACTGTAACCCAACTTCAATTTGTTGTTTGGGC
TAAACAAGTTAGTGCACTTGTTGTTTCTCTTGAACATCGTTATTATGGTGCTAGTTTTGT
AACTGAAGACCTTTCACTTGAAAATTTACAATGGTTAAATTCAGCACAAGCATTAGCAGA
TAATGCAGTTTTTAGAAATTTTGTAGCACAACAATATAATGTACCAAAAGAATCAAAATG
GATTTCATTTGGTGGTAGTTATTCAGGAGCATTAACTTCATGGTTTAGAATTAAATATCC
ACATTTAGTTGATGCCACCATTGCATCAAGTGCACCAGTTA----------
Length of 5' end seq. 521
3' end seq. ID VFB720Z
3' end seq. 
>VFB720Z.Seq
----------TGTGTTGAATTTGGTTATTATCAATCAAGTGATGCTCCATCTGCAAATCA
ACCATTTGGTAATTTATTCCCATTCCAACCATATCAAATTCAACAATGTGCCGATAGTTT
TGGAATTCNAAATATGTATCCAAACGTTAATTGGACAATCACTGAATATGGTGGTATTAA
TCCAGAACCATCCTCTGTTGATAATACCTTATATGTAAATGGTTCAAATGATGAATGGCA
CAATCTTGCAATCTTACCAGGTAATGCAAATGCTAAAAATACTTTATACATTATTGGTAC
NAGTCNTTGTGCTGATATGATGATTCCAACTTCAGTATCACCTTCCAATTTCGGGNACAA
GCA
Length of 3' end seq. 353
Connected seq. ID VFB720P
Connected seq. 
>VFB720P.Seq
TTTAAAAAATCAATTAAAATTTAGACATTTTGATGATAGTTTAGATTATGATGCAATCAA
TTATCAATGGTTCACTCAAAGTGTTGATCATTTTAATCCAGCAAACCCAACAACTTTCCA
ACAAAGATATTTAATTAATGACCAATATTGGGATGGTACAGGTCCAGTTTTTATCATGAT
TAATGGTGAAGGTCCAATGGATATTAATACTGTAACCCAACTTCAATTTGTTGTTTGGGC
TAAACAAGTTAGTGCACTTGTTGTTTCTCTTGAACATCGTTATTATGGTGCTAGTTTTGT
AACTGAAGACCTTTCACTTGAAAATTTACAATGGTTAAATTCAGCACAAGCATTAGCAGA
TAATGCAGTTTTTAGAAATTTTGTAGCACAACAATATAATGTACCAAAAGAATCAAAATG
GATTTCATTTGGTGGTAGTTATTCAGGAGCATTAACTTCATGGTTTAGAATTAAATATCC
ACATTTAGTTGATGCCACCATTGCATCAAGTGCACCAGTTA----------TGTGTTGAA
TTTGGTTATTATCAATCAAGTGATGCTCCATCTGCAAATCAACCATTTGGTAATTTATTC
CCATTCCAACCATATCAAATTCAACAATGTGCCGATAGTTTTGGAATTCNAAATATGTAT
CCAAACGTTAATTGGACAATCACTGAATATGGTGGTATTAATCCAGAACCATCCTCTGTT
GATAATACCTTATATGTAAATGGTTCAAATGATGAATGGCACAATCTTGCAATCTTACCA
GGTAATGCAAATGCTAAAAATACTTTATACATTATTGGTACNAGTCNTTGTGCTGATATG
ATGATTCCAACTTCAGTATCACCTTCCAATTTCGGGNACAAGCA
Length of connected seq. 874
Full length Seq ID -
Full length Seq. -
Length of full length seq. -