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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFB718
Library VF
(Link to library)
Clone ID VFB718
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16382-1
Original site URL
Representative seq. ID VFB718P
(Link to Original site)
Representative DNA sequence 
>VFB718 (VFB718Q) /CSM/VF/VFB7-A/VFB718Q.Seq.d/
GNAAAAAAAACTTAAATAAATAATAATAATATAATAAAATGGATGGTGAAGATGTTCAAG
CTTTAGTTATTGATAACGGTTCTGGTATGTGTAAAGCCGGTTTTGCTGGTGATGATGCTC
CACGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGACACACTGGTGTTATGGTCGGTA
TGGGTCAAAAGGATTCATATGTAGGTGATGAAGCTCAATCAAAAAGAGGTATCTTAACTC
TCAAATCTCCAATTGAACATGGAATTGTCACCAATTGGGATGATATGGAAAAGATCTGNC
ATCATACTTTCTACAATGAACTTNNTGTTGCACCANAAGAACATCCATTTCTTTTAACCN
AXXXXXXXXXXTAACTGAACGTGGTTACTCATTCACCACCACCGCCGAAAGAGAAATTGT
CAGAGATATCAAAAAAAAATTAGCATACGTCGCCCTCGACTTTGAAGCTGAAATGCAAAC
TGCTGCCTCATCATCAGCATTAGAGAAATCATATGAATTACCAGATGGTCAAGTTATCAC
AATTGGTAATGAACGTTTCCGTTGTCCAGAAGCTCTCTTCCAACCATCATTCTTAGGTAT
GGAATCAGCTGGTATTCACGAAACCACATACAATTCAATCATGAAATGTGATGTCGGTAT
TCGTAAAGATTTATATGGTAATGTTGTATTATCAGGTGGTACAACTATGTTCCCAGGTAT
TGCAGATCGTATGAACAAAGAATTAACAGCATTAGCACCATCAACAATGAAAATCAAAAT
CATTGCACCACCAGAACGTAAATACTCAGTCTGGATTGGTGGATCTATTTTAGCTTCACT
CTCAACTTTCCAACAAATGTGGATTTCAAAAGAAGAATATGACGAATCTGGTCCATCAAT
TGTTCACAGAAAATGTTTCTAAATTAATTAAAAAAATTTAGTGATGAAAGTGCTTCTCAC
ACAAAAATTATTATATATGTACAATAATAACAATAAAAACCCAATAAAATATAACTTTTT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
kknlnk***YNKMDGEDVQALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHTGVMVGM
GQKDSYVGDEAQSKRGILTLKSPIEHGIVTNWDDMEKIXHHTFYNELXVAPXEHPFLLT-
--

---TERGYSFTTTAEREIVRDIKKKLAYVALDFEAEMQTAASSSALEKSYELPDGQVITI
GNERFRCPEALFQPSFLGMESAGIHETTYNSIMKCDVGIRKDLYGNVVLSGGTTMFPGIA
DRMNKELTALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKEEYDESGPSIV
HRKCF*in*knlvmkvlltqkllymynnnnknpikynf
Translated Amino Acid sequence (All Frames) 
Frame A:
xkkt*innnniikwmvkmfkl*llitvlvcvkpvllvmmlhvlfshqllvvqdtlvlwsv
wvkrihm*vmklnqkevs*lsnlqlnmelspigmiwkrsxiilstmnxxlhxknihff*p
---

---*lnvvthsppppkeklseiskkn*htspstlklkckllphhqh*rnhmnyqmvklsq
lvmnvsvvqklssnhhs*vwnqlvftkphtiqs*nvmsvfvkiymvmlyyqvvqlcsqvl
qiv*tkn*qh*hhqq*kskslhhqnvntqsglvdlf*lhsqlsnkcgfqkknmtnlvhql
ftenvsklikki***kcfshknyyictiitiktq*nitf

Frame B:
xkklk*iiii**ngw*rcssfsy**rfwyv*srfcw**cstccfpincwsskthwcygry
gskgficr**ssikkrylnsqisn*twnchqlg*ygkdlxsyflq*txcctxrtsisfnx
---

---n*twllihhhrrkrncqryqkkisirrprl*s*nanccliisireii*itrwssyhn
w**tfplsrsslptiilrygiswysrnhiqfnhem*crys*rfiw*cciirwynyvpryc
rsyeqrinsistinnenqnhcttrt*ilsldwwiyfsftlnfptnvdfkrri*riwsinc
sqkmfln*lkkfsdesashtkiiiyvq**q*kpnki*lf

Frame C:
kknlnk***YNKMDGEDVQALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHTGVMVGM
GQKDSYVGDEAQSKRGILTLKSPIEHGIVTNWDDMEKIXHHTFYNELXVAPXEHPFLLT-
--

---TERGYSFTTTAEREIVRDIKKKLAYVALDFEAEMQTAASSSALEKSYELPDGQVITI
GNERFRCPEALFQPSFLGMESAGIHETTYNSIMKCDVGIRKDLYGNVVLSGGTTMFPGIA
DRMNKELTALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKEEYDESGPSIV
HRKCF*in*knlvmkvlltqkllymynnnnknpikynf
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFB718 (VFB718Q) /CSM/VF/VFB7-A/VFB718Q.Seq.d/                       1758   0.0
VHP652 (VHP652Q) /CSM/VH/VHP6-C/VHP652Q.Seq.d/                       1183   0.0
VFN229 (VFN229Q) /CSM/VF/VFN2-B/VFN229Q.Seq.d/                       1183   0.0
VFJ430 (VFJ430Q) /CSM/VF/VFJ4-B/VFJ430Q.Seq.d/                       1183   0.0
VFG345 (VFG345Q) /CSM/VF/VFG3-B/VFG345Q.Seq.d/                       1183   0.0
VFE476 (VFE476Q) /CSM/VF/VFE4-D/VFE476Q.Seq.d/                       1183   0.0
AFF326 (AFF326Q) /CSM/AF/AFF3-B/AFF326Q.Seq.d/                       1176   0.0
VFJ878 (VFJ878Q) /CSM/VF/VFJ8-D/VFJ878Q.Seq.d/                       1170   0.0
VFN368 (VFN368Q) /CSM/VF/VFN3-C/VFN368Q.Seq.d/                       1164   0.0
VFA769 (VFA769Q) /CSM/VF/VFA7-C/VFA769Q.Seq.d/                       1154   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC116956|AC116956.2 Dictyostelium discoideum chromosome 2 map 1418423-1684967 strain AX4, complete sequence.   963  0.0  6
X03282|X03282.1 dictyostelium discoideum gene for actin A12.     926  0.0  3
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.   765  0.0  7
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.   761  0.0  5
M14146|M14146.1 D.discoideum actin 15 gene, complete cds.        724  0.0  4
AC115577|AC115577.2 Dictyostelium discoideum chromosome 2 map 4657875-4914984 strain AX4, complete sequence.   724  0.0  12
AC115579|AC115579.2 Dictyostelium discoideum chromosome 2 map 4915084-5005461 strain AX4, complete sequence.   724  0.0  6
AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.   666  0.0  6
X03281|X03281.1 Dictyostelium discoideum gene for actin A8.      656  0.0  3
X03283|X03283.1 Dictyostelium discoideum gene for actin A3-S1.   648  0.0  3
dna update 2003. 9. 6
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AL160004_3(AL160004|pid:none) Human DNA sequence from clone RP5-...   315   e-135
A03000(A03000)actin 3 - fruit fly (Drosophila melanogaster) (fra...   283   e-128
(Q54H30) RecName: Full=Major actin; AltName: Full=Actin-1; AltNa...   360   3e-98
X03282_1(X03282|pid:none) dictyostelium discoideum gene for acti...   360   3e-98
B23412(B23412)actin 12 - slime mold (Dictyostelium discoideum)        360   3e-98
ATDO(A93223;A92871;S67999;A03004)actin - slime mold (Dictyosteli...   356   6e-97
(P02576) RecName: Full=Actin, plasmodial isoform;  &ATFY(A29044;...   356   6e-97
(Q553U6) RecName: Full=Putative actin-22;  &AC116305_3(AC116305|...   356   6e-97
(P07829) RecName: Full=Actin-3; AltName: Full=Actin-3-sub 1;          353   4e-96
AF508263_1(AF508263|pid:none) Pyrocystis lunula actin mRNA, comp...   353   5e-96
protein update 2009. 6.17
PSORT 

 psg: 0.75  gvh: 0.34  alm: 0.46  top: 0.53  tms: 0.00  mit: 0.16  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: nuclear
	 32.0 %: cytoplasmic
	 24.0 %: cytoskeletal
	  8.0 %: mitochondrial

>> prediction for VFB718 is nuc
5' end seq. ID VFB718F
5' end seq. 
>VFB718F.Seq
GNAAAAAAAACTTAAATAAATAATAATAATATAATAAAATGGATGGTGAAGATGTTCAAG
CTTTAGTTATTGATAACGGTTCTGGTATGTGTAAAGCCGGTTTTGCTGGTGATGATGCTC
CACGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGACACACTGGTGTTATGGTCGGTA
TGGGTCAAAAGGATTCATATGTAGGTGATGAAGCTCAATCAAAAAGAGGTATCTTAACTC
TCAAATCTCCAATTGAACATGGAATTGTCACCAATTGGGATGATATGGAAAAGATCTGNC
ATCATACTTTCTACAATGAACTTNNTGTTGCACCANAAGAACATCCATTTCTTTTAACCN
A----------
Length of 5' end seq. 361
3' end seq. ID VFB718Z
3' end seq. 
>VFB718Z.Seq
----------TAACTGAACGTGGTTACTCATTCACCACCACCGCCGAAAGAGAAATTGTC
AGAGATATCAAAAAAAAATTAGCATACGTCGCCCTCGACTTTGAAGCTGAAATGCAAACT
GCTGCCTCATCATCAGCATTAGAGAAATCATATGAATTACCAGATGGTCAAGTTATCACA
ATTGGTAATGAACGTTTCCGTTGTCCAGAAGCTCTCTTCCAACCATCATTCTTAGGTATG
GAATCAGCTGGTATTCACGAAACCACATACAATTCAATCATGAAATGTGATGTCGGTATT
CGTAAAGATTTATATGGTAATGTTGTATTATCAGGTGGTACAACTATGTTCCCAGGTATT
GCAGATCGTATGAACAAAGAATTAACAGCATTAGCACCATCAACAATGAAAATCAAAATC
ATTGCACCACCAGAACGTAAATACTCAGTCTGGATTGGTGGATCTATTTTAGCTTCACTC
TCAACTTTCCAACAAATGTGGATTTCAAAAGAAGAATATGACGAATCTGGTCCATCAATT
GTTCACAGAAAATGTTTCTAAATTAATTAAAAAAATTTAGTGATGAAAGTGCTTCTCACA
CAAAAATTATTATATATGTACAATAATAACAATAAAAACCCAATAAAATATAACTTTTT
Length of 3' end seq. 649
Connected seq. ID VFB718P
Connected seq. 
>VFB718P.Seq
GNAAAAAAAACTTAAATAAATAATAATAATATAATAAAATGGATGGTGAAGATGTTCAAG
CTTTAGTTATTGATAACGGTTCTGGTATGTGTAAAGCCGGTTTTGCTGGTGATGATGCTC
CACGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGACACACTGGTGTTATGGTCGGTA
TGGGTCAAAAGGATTCATATGTAGGTGATGAAGCTCAATCAAAAAGAGGTATCTTAACTC
TCAAATCTCCAATTGAACATGGAATTGTCACCAATTGGGATGATATGGAAAAGATCTGNC
ATCATACTTTCTACAATGAACTTNNTGTTGCACCANAAGAACATCCATTTCTTTTAACCN
A----------TAACTGAACGTGGTTACTCATTCACCACCACCGCCGAAAGAGAAATTGT
CAGAGATATCAAAAAAAAATTAGCATACGTCGCCCTCGACTTTGAAGCTGAAATGCAAAC
TGCTGCCTCATCATCAGCATTAGAGAAATCATATGAATTACCAGATGGTCAAGTTATCAC
AATTGGTAATGAACGTTTCCGTTGTCCAGAAGCTCTCTTCCAACCATCATTCTTAGGTAT
GGAATCAGCTGGTATTCACGAAACCACATACAATTCAATCATGAAATGTGATGTCGGTAT
TCGTAAAGATTTATATGGTAATGTTGTATTATCAGGTGGTACAACTATGTTCCCAGGTAT
TGCAGATCGTATGAACAAAGAATTAACAGCATTAGCACCATCAACAATGAAAATCAAAAT
CATTGCACCACCAGAACGTAAATACTCAGTCTGGATTGGTGGATCTATTTTAGCTTCACT
CTCAACTTTCCAACAAATGTGGATTTCAAAAGAAGAATATGACGAATCTGGTCCATCAAT
TGTTCACAGAAAATGTTTCTAAATTAATTAAAAAAATTTAGTGATGAAAGTGCTTCTCAC
ACAAAAATTATTATATATGTACAATAATAACAATAAAAACCCAATAAAATATAACTTTTT
Length of connected seq. 1010
Full length Seq ID -
Full length Seq. -
Length of full length seq. -