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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFB710
Library VF
(Link to library)
Clone ID VFB710
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16382-1
Original site URL
Representative seq. ID VFB710P
(Link to Original site)
Representative DNA sequence 
>VFB710 (VFB710Q) /CSM/VF/VFB7-A/VFB710Q.Seq.d/
ACGGTGAAGATGTTCAAGCTTTAGTTATCGATAACGGTTCTGGTATGTGTAAAGCCGGNT
TTGCTGGTGATGATGCCCCACGTGCTGTTTTCCCATCAATTGTTGGTCGNCCAAGACACA
CTGXXXXXXXXXXTTCAGGTGATGGTGTCTCCCACACTGTACCAATCTATNAAGGTTATG
CTTTACCACATGCCATCTTACGTTTAGACTTAGCTGGTCGTGATCTTACCGATTACATGA
TGAAAATCTTAACTGAACGTGGTTACTCATTCACCACCACTGCCGAAAGAGAAATCGTCA
GAGATATCAAAGAAAAATTAGCCTACGTCGCCCTCGACTTTGAAGCTGAAATGCAAACTG
CTGCCTCATCATCAGCCCTCGAAAAATCATACGAATTACCAGACGGTCAAGTCATCACCA
TCGGTAATGAACGTTTCCGTTGCCCAGAAGCCCTCTTCCAACCATCATTCTTAGGTATGG
AATCTGCTGGTATCCACGAAACCACCTACAACTCCATCATGAAATGTGATGTTGATATCC
GTAAAGATTTATACGGTAACGTCGTCTTATCCGGTGGTACAACTATGTTCCCAGGTATTG
CTGATCGTATGAACAAAGAATTAACTGCTTTAGCCCCATCAACCATGAAAATTAAAATCA
TTGCTCCACCAGAACGTAAATACTCTGTCTGGATTGGTGGATCTATCTTAGCTTCACTCT
CAACTTTCCAACAAATGTGGATCTCCAAAGAAGAATACGACGAATCAGGTCCATCAATTG
TTCACAGAAAATGTTTCTAAATTATTTAATAAATAATAAAAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
GEDVQALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHT---

---SGDGVSHTVPIYXGYALPHAILRLDLAGRDLTDYMMKILTERGYSFTTTAEREIVRD
IKEKLAYVALDFEAEMQTAASSSALEKSYELPDGQVITIGNERFRCPEALFQPSFLGMES
AGIHETTYNSIMKCDVDIRKDLYGNVVLSGGTTMFPGIADRMNKELTALAPSTMKIKIIA
PPERKYSVWIGGSILASLSTFQQMWISKEEYDESGPSIVHRKCF*ii**iik
Translated Amino Acid sequence (All Frames) 
Frame A:
tvkmfkl*lsitvlvcvkpxllvmmphvlfshqllvxqdtl---

---fr*wclphctnlxrlcfttchltfrlsws*syrlhdenln*twllihhhcrkrnrqr
yqrkislrrprl*s*nanccliisprkiiritrrsshhhr**tfplprsplptiilrygi
cwyprnhlqlhhem*c*yp*rfir*rrlirwynyvpryc*syeqrincfspinhen*nhc
strt*ilcldwwiylsftlnfptnvdlqrrirrirsincsqkmflnylinnk

Frame B:
r*rcssfsyr*rfwyv*srxcw**cptccfpincwsxkth---

---SGDGVSHTVPIYXGYALPHAILRLDLAGRDLTDYMMKILTERGYSFTTTAEREIVRD
IKEKLAYVALDFEAEMQTAASSSALEKSYELPDGQVITIGNERFRCPEALFQPSFLGMES
AGIHETTYNSIMKCDVDIRKDLYGNVVLSGGTTMFPGIADRMNKELTALAPSTMKIKIIA
PPERKYSVWIGGSILASLSTFQQMWISKEEYDESGPSIVHRKCF*ii**iik

Frame C:
GEDVQALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHT---

---qvmvsptlyqsxkvmlyhmpsyv*t*lvvilpit**ks*lnvvthspplpkekssei
skkn*ptspstlklkckllphhqpsknhtnyqtvksspsvmnvsvaqkpssnhhs*vwnl
lvstkppttps*nvmlisvkiytvtssypvvqlcsqvlliv*tkn*ll*phqp*klksll
hqnvntlsglvdls*lhsqlsnkcgspkknttnqvhqlftenvsklfnk**k
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFB710 (VFB710Q) /CSM/VF/VFB7-A/VFB710Q.Seq.d/                       1552   0.0
VFM550 (VFM550Q) /CSM/VF/VFM5-C/VFM550Q.Seq.d/                       1352   0.0
VFL746 (VFL746Q) /CSM/VF/VFL7-B/VFL746Q.Seq.d/                       1352   0.0
VFK530 (VFK530Q) /CSM/VF/VFK5-B/VFK530Q.Seq.d/                       1352   0.0
VFI577 (VFI577Q) /CSM/VF/VFI5-D/VFI577Q.Seq.d/                       1352   0.0
VFI557 (VFI557Q) /CSM/VF/VFI5-C/VFI557Q.Seq.d/                       1352   0.0
VFI526 (VFI526Q) /CSM/VF/VFI5-B/VFI526Q.Seq.d/                       1352   0.0
VFI515 (VFI515Q) /CSM/VF/VFI5-A/VFI515Q.Seq.d/                       1352   0.0
VFI483 (VFI483Q) /CSM/VF/VFI4-D/VFI483Q.Seq.d/                       1352   0.0
VFH708 (VFH708Q) /CSM/VF/VFH7-A/VFH708Q.Seq.d/                       1352   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

X03281|X03281.1 Dictyostelium discoideum gene for actin A8.     1308  0.0  2
AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.  1231  0.0  4
M14146|M14146.1 D.discoideum actin 15 gene, complete cds.       1178  0.0  2
AC115579|AC115579.2 Dictyostelium discoideum chromosome 2 map 4915084-5005461 strain AX4, complete sequence.  1178  0.0  3
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.  1072  0.0  5
AC115577|AC115577.2 Dictyostelium discoideum chromosome 2 map 4657875-4914984 strain AX4, complete sequence.  1021  0.0  12
AC116956|AC116956.2 Dictyostelium discoideum chromosome 2 map 1418423-1684967 strain AX4, complete sequence.   993  0.0  5
X03282|X03282.1 dictyostelium discoideum gene for actin A12.     991  0.0  2
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.   835  0.0  2
X03283|X03283.1 Dictyostelium discoideum gene for actin A3-S1.   706  0.0  2
dna update 2003. 9. 6
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54H30) RecName: Full=Major actin; AltName: Full=Actin-1; AltNa...   441   e-122
X03282_1(X03282|pid:none) dictyostelium discoideum gene for acti...   441   e-122
B23412(B23412)actin 12 - slime mold (Dictyostelium discoideum)        441   e-122
ATDO(A93223;A92871;S67999;A03004)actin - slime mold (Dictyosteli...   436   e-121
(Q553U6) RecName: Full=Putative actin-22;  &AC116305_3(AC116305|...   436   e-121
(P02576) RecName: Full=Actin, plasmodial isoform;  &ATFY(A29044;...   435   e-121
AF508263_1(AF508263|pid:none) Pyrocystis lunula actin mRNA, comp...   433   e-120
(P07829) RecName: Full=Actin-3; AltName: Full=Actin-3-sub 1;          432   e-120
AY615868_1(AY615868|pid:none) Amastigomonas marina actin gene, p...   430   e-119
AY294154_1(AY294154|pid:none) Thecamoeba similis actin mRNA, com...   429   e-119
protein update 2009. 6.17
PSORT 

 psg: 0.75  gvh: 0.34  alm: 0.46  top: 0.53  tms: 0.00  mit: 0.18  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: cytoplasmic
	 36.0 %: nuclear
	 24.0 %: cytoskeletal
	  4.0 %: mitochondrial

>> prediction for VFB710 is cyt
5' end seq. ID VFB710F
5' end seq. 
>VFB710F.Seq
ACGGTGAAGATGTTCAAGCTTTAGTTATCGATAACGGTTCTGGTATGTGTAAAGCCGGNT
TTGCTGGTGATGATGCCCCACGTGCTGTTTTCCCATCAATTGTTGGTCGNCCAAGACACA
CTG----------
Length of 5' end seq. 123
3' end seq. ID VFB710Z
3' end seq. 
>VFB710Z.Seq
----------TTCAGGTGATGGTGTCTCCCACACTGTACCAATCTATNAAGGTTATGCTT
TACCACATGCCATCTTACGTTTAGACTTAGCTGGTCGTGATCTTACCGATTACATGATGA
AAATCTTAACTGAACGTGGTTACTCATTCACCACCACTGCCGAAAGAGAAATCGTCAGAG
ATATCAAAGAAAAATTAGCCTACGTCGCCCTCGACTTTGAAGCTGAAATGCAAACTGCTG
CCTCATCATCAGCCCTCGAAAAATCATACGAATTACCAGACGGTCAAGTCATCACCATCG
GTAATGAACGTTTCCGTTGCCCAGAAGCCCTCTTCCAACCATCATTCTTAGGTATGGAAT
CTGCTGGTATCCACGAAACCACCTACAACTCCATCATGAAATGTGATGTTGATATCCGTA
AAGATTTATACGGTAACGTCGTCTTATCCGGTGGTACAACTATGTTCCCAGGTATTGCTG
ATCGTATGAACAAAGAATTAACTGCTTTAGCCCCATCAACCATGAAAATTAAAATCATTG
CTCCACCAGAACGTAAATACTCTGTCTGGATTGGTGGATCTATCTTAGCTTCACTCTCAA
CTTTCCAACAAATGTGGATCTCCAAAGAAGAATACGACGAATCAGGTCCATCAATTGTTC
ACAGAAAATGTTTCTAAATTATTTAATAAATAATAAAAA
Length of 3' end seq. 689
Connected seq. ID VFB710P
Connected seq. 
>VFB710P.Seq
ACGGTGAAGATGTTCAAGCTTTAGTTATCGATAACGGTTCTGGTATGTGTAAAGCCGGNT
TTGCTGGTGATGATGCCCCACGTGCTGTTTTCCCATCAATTGTTGGTCGNCCAAGACACA
CTG----------TTCAGGTGATGGTGTCTCCCACACTGTACCAATCTATNAAGGTTATG
CTTTACCACATGCCATCTTACGTTTAGACTTAGCTGGTCGTGATCTTACCGATTACATGA
TGAAAATCTTAACTGAACGTGGTTACTCATTCACCACCACTGCCGAAAGAGAAATCGTCA
GAGATATCAAAGAAAAATTAGCCTACGTCGCCCTCGACTTTGAAGCTGAAATGCAAACTG
CTGCCTCATCATCAGCCCTCGAAAAATCATACGAATTACCAGACGGTCAAGTCATCACCA
TCGGTAATGAACGTTTCCGTTGCCCAGAAGCCCTCTTCCAACCATCATTCTTAGGTATGG
AATCTGCTGGTATCCACGAAACCACCTACAACTCCATCATGAAATGTGATGTTGATATCC
GTAAAGATTTATACGGTAACGTCGTCTTATCCGGTGGTACAACTATGTTCCCAGGTATTG
CTGATCGTATGAACAAAGAATTAACTGCTTTAGCCCCATCAACCATGAAAATTAAAATCA
TTGCTCCACCAGAACGTAAATACTCTGTCTGGATTGGTGGATCTATCTTAGCTTCACTCT
CAACTTTCCAACAAATGTGGATCTCCAAAGAAGAATACGACGAATCAGGTCCATCAATTG
TTCACAGAAAATGTTTCTAAATTATTTAATAAATAATAAAAA
Length of connected seq. 812
Full length Seq ID -
Full length Seq. -
Length of full length seq. -