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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFB707
Library VF
(Link to library)
Clone ID VFB707
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U09871-1
Original site URL
Representative seq. ID VFB707P
(Link to Original site)
Representative DNA sequence 
>VFB707 (VFB707Q) /CSM/VF/VFB7-A/VFB707Q.Seq.d/
TTTTCAACATTTCATTCATATATTTGTATATACTTATATAAGATGTCACTCTTTAATTCA
GCTAATAAAGTTATTGGAGGAGTTTTAAAATTTAATCCAAGTAAATATCAAGTTAGATAT
TTAAATTTACATGAATATCAAAGTAAATCATTAATGGATAAATATGGAGTTAATACACAA
AAATGGAGAGTTGTAACAAAAGCATCAGATGCCATCAAAGCAGCATXXXXXXXXXXCTAA
AAAACCTCGCTGAGAAATTAGGTTTCACCGGTGAGAAAGCAAAGATCGCTCAACAACAAA
TGGAAAACCTCTATCAATTATTCATCAAGAGTGATGCAACTCAAGTCGAGATTAACCCAT
TCGCTGAAACCACCGATGGTCAAGTCATTTGTATGGATGCTAAAATCAATTTCGATGACA
ATGCCTCATTCCGTCAAAAGGAGATTTTCGAAATGAGAGATACCGCCGAAGAGGATCCAC
GTGAGGTTGAAGCTGGTAAATTTGGTCTCAATTACATTGGTCTCGATGGTAACATTGGTT
GTATGGTTAATGGTGCTGGTTTGGCTATGGCCACCATGGATATCATTAAATTAAAGGGTG
GTATCCCTGCTAATTTCCTCGATGTAGGTGGTAGTGCCTCTGAACAAGCCGTCACTGAAG
CCTTCAAAATCCTCACTAAAGATCCAAGAGTTAAATGTTTACTCGTAAACATTTTCGGTG
GTATCATGAAATGTGATATCATTGCCTCTGGTATCGTAAATGCTTCAAAACAAGTTGGTC
TTAAAATTCCATTAGTTGTACGTTTAGAAGGTACAAATGTAAACATTGGTAAAGAAATCT
GGAAAAATCGGTTTAAATATACTTCTGCTAGNATTTAG
sequence update 2001. 6. 1
Translated Amino Acid sequence 
FSTFHSYICIYLYKMSLFNSANKVIGGVLKFNPSKYQVRYLNLHEYQSKSLMDKYGVNTQ
KWRVVTKASDAIKAA---

---LKNLAEKLGFTGEKAKIAQQQMENLYQLFIKSDATQVEINPFAETTDGQVICMDAKI
NFDDNASFRQKEIFEMRDTAEEDPREVEAGKFGLNYIGLDGNIGCMVNGAGLAMATMDII
KLKGGIPANFLDVGGSASEQAVTEAFKILTKDPRVKCLLVNIFGGIMKCDIIASGIVNAS
KQVGLKIPLVVRLEGTNVNIGKEIWKNRFKYTSAXI*
Translated Amino Acid sequence (All Frames) 
Frame A:
FSTFHSYICIYLYKMSLFNSANKVIGGVLKFNPSKYQVRYLNLHEYQSKSLMDKYGVNTQ
KWRVVTKASDAIKAA---

---LKNLAEKLGFTGEKAKIAQQQMENLYQLFIKSDATQVEINPFAETTDGQVICMDAKI
NFDDNASFRQKEIFEMRDTAEEDPREVEAGKFGLNYIGLDGNIGCMVNGAGLAMATMDII
KLKGGIPANFLDVGGSASEQAVTEAFKILTKDPRVKCLLVNIFGGIMKCDIIASGIVNAS
KQVGLKIPLVVRLEGTNVNIGKEIWKNRFKYTSAXI*

Frame B:
fqhfihifvytyirchsliqliklleef*nliqvnikldi*iymnikvnh*winmelihk
ngel*qkhqmpskqh---

---*ktslrn*vspvrkqrslnnkwktsinyssrvmqlksrlthslkppmvksfvwmlks
ismtmphsvkrrfsk*eippkrihvrlklvnlvsitlvsmvtlvvwlmvlvwlwppwisl
n*rvvsllissm*vvvplnkpslkpsksslkiqelnvys*tfsvvs*nvislplvs*mlq
nklvlkfh*lyv*kvqm*tlvkksgkiglnilllxf

Frame C:
fnisfiylyili*dvtl*fs**sywrsfki*sk*iss*ifkft*isk*iing*iws*ytk
mescnksirchqss---

---kkpr*eirfhr*eskdrsttngkplsiihqe*cnssrd*pir*nhrwsshlygc*nq
fr*qclipskgdfrneryrrrgst*g*sw*iwsqlhwsrw*hwlyg*wcwfgyghhgyh*
ikgwypc*fprcrw*cl*tsrh*slqnph*rsks*mftrkhfrwyhem*yhclwyrkcfk
tsws*nsisctfrrykckhw*rnleksv*iyfc*xl
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFB707 (VFB707Q) /CSM/VF/VFB7-A/VFB707Q.Seq.d/                       1675   0.0
AFK346 (AFK346Q) /CSM/AF/AFK3-B/AFK346Q.Seq.d/                       1261   0.0
VFJ843 (VFJ843Q) /CSM/VF/VFJ8-B/VFJ843Q.Seq.d/                       1247   0.0
AFI578 (AFI578Q) /CSM/AF/AFI5-D/AFI578Q.Seq.d/                       1233   0.0
AFJ372 (AFJ372Q) /CSM/AF/AFJ3-C/AFJ372Q.Seq.d/                       1231   0.0
AFD689 (AFD689Q) /CSM/AF/AFD6-D/AFD689Q.Seq.d/                       1231   0.0
VFA723 (VFA723Q) /CSM/VF/VFA7-A/VFA723Q.Seq.d/                       1225   0.0
AFI855 (AFI855Q) /CSM/AF/AFI8-C/AFI855Q.Seq.d/                       1225   0.0
VFF668 (VFF668Q) /CSM/VF/VFF6-C/VFF668Q.Seq.d/                       1219   0.0
VFD829 (VFD829Q) /CSM/VF/VFD8-B/VFD829Q.Seq.d/                       1219   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.  1191  0.0  4
AC115594|AC115594.2 Dictyostelium discoideum chromosome 2 map 4071862-4101005 strain AX4, complete sequence.    62  2e-22  6
X84222|X84222.2 Neocallimastix frontalis mRNA for beta-succinyl CoA synthetase precursor.    74  3e-21  4
AF493055|AF493055.1 Neocallimastix patriciarum succinyl-CoA synthetase, beta subunit (scsB) mRNA, complete cds.    74  4e-21  4
AX621832|AX621832.1 Sequence 4795 from Patent WO02094868.         52  3e-19  4
AR354123|AR354123.1 Sequence 241 from patent US 6593114.          52  2e-17  4
BJ519880|BJ519880.1 Oryzias latipes cDNA clone:MF01SSB014A03, 5' end single read.    70  2e-16  2
BJ532800|BJ532800.1 Oryzias latipes cDNA clone:MF01SSB015A03, 3' end single read.    70  2e-16  2
BJ520215|BJ520215.1 Oryzias latipes cDNA clone:MF01SSB015A03, 5' end single read.    70  2e-16  2
CC820121|CC820121.1 1O0001G05R Oxytricha plasmid UUGC1O library Sterkiella histriomuscorum genomic clone UUGC1O0001G05 R, genomic survey sequence.    84  8e-16  2
dna update 2003. 9. 6
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q6K9N6) RecName: Full=Succinyl-CoA ligase [GDP-forming] subunit...   279   8e-74
BT039713_1(BT039713|pid:none) Zea mays full-length cDNA clone ZM...   276   6e-73
EF530042_1(EF530042|pid:none) Caragana jubata succinyl-CoA ligas...   275   2e-72
AC115594_9(AC115594|pid:none) Dictyostelium discoideum chromosom...   274   2e-72
(Q55AI5) RecName: Full=Succinyl-CoA ligase [ADP-forming] subunit...   274   2e-72
AK221149_1(AK221149|pid:none) Arabidopsis thaliana mRNA for succ...   272   9e-72
(O82662) RecName: Full=Succinyl-CoA ligase [GDP-forming] subunit...   272   9e-72
AY087596_1(AY087596|pid:none) Arabidopsis thaliana clone 36904 m...   270   5e-71
DQ200398_1(DQ200398|pid:none) Solanum tuberosum clone 071C05 suc...   269   1e-70
DQ294279_1(DQ294279|pid:none) Solanum tuberosum clone 180E05 suc...   269   1e-70
protein update 2009. 6.17
PSORT 

 psg: 0.86  gvh: 0.37  alm: 0.33  top: 0.57  tms: 0.00  mit: 0.32  mip: 0.07
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 24.0 %: mitochondrial
	 16.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: plasma membrane
	  4.0 %: endoplasmic reticulum

>> prediction for VFB707 is cyt
5' end seq. ID VFB707F
5' end seq. 
>VFB707F.Seq
TTTTCAACATTTCATTCATATATTTGTATATACTTATATAAGATGTCACTCTTTAATTCA
GCTAATAAAGTTATTGGAGGAGTTTTAAAATTTAATCCAAGTAAATATCAAGTTAGATAT
TTAAATTTACATGAATATCAAAGTAAATCATTAATGGATAAATATGGAGTTAATACACAA
AAATGGAGAGTTGTAACAAAAGCATCAGATGCCATCAAAGCAGCAT----------
Length of 5' end seq. 226
3' end seq. ID VFB707Z
3' end seq. 
>VFB707Z.Seq
----------CTAAAAAACCTCGCTGAGAAATTAGGTTTCACCGGTGAGAAAGCAAAGAT
CGCTCAACAACAAATGGAAAACCTCTATCAATTATTCATCAAGAGTGATGCAACTCAAGT
CGAGATTAACCCATTCGCTGAAACCACCGATGGTCAAGTCATTTGTATGGATGCTAAAAT
CAATTTCGATGACAATGCCTCATTCCGTCAAAAGGAGATTTTCGAAATGAGAGATACCGC
CGAAGAGGATCCACGTGAGGTTGAAGCTGGTAAATTTGGTCTCAATTACATTGGTCTCGA
TGGTAACATTGGTTGTATGGTTAATGGTGCTGGTTTGGCTATGGCCACCATGGATATCAT
TAAATTAAAGGGTGGTATCCCTGCTAATTTCCTCGATGTAGGTGGTAGTGCCTCTGAACA
AGCCGTCACTGAAGCCTTCAAAATCCTCACTAAAGATCCAAGAGTTAAATGTTTACTCGT
AAACATTTTCGGTGGTATCATGAAATGTGATATCATTGCCTCTGGTATCGTAAATGCTTC
AAAACAAGTTGGTCTTAAAATTCCATTAGTTGTACGTTTAGAAGGTACAAATGTAAACAT
TGGTAAAGAAATCTGGAAAAATCGGTTTAAATATACTTCTGCTAGNATTTAG
Length of 3' end seq. 642
Connected seq. ID VFB707P
Connected seq. 
>VFB707P.Seq
TTTTCAACATTTCATTCATATATTTGTATATACTTATATAAGATGTCACTCTTTAATTCA
GCTAATAAAGTTATTGGAGGAGTTTTAAAATTTAATCCAAGTAAATATCAAGTTAGATAT
TTAAATTTACATGAATATCAAAGTAAATCATTAATGGATAAATATGGAGTTAATACACAA
AAATGGAGAGTTGTAACAAAAGCATCAGATGCCATCAAAGCAGCAT----------CTAA
AAAACCTCGCTGAGAAATTAGGTTTCACCGGTGAGAAAGCAAAGATCGCTCAACAACAAA
TGGAAAACCTCTATCAATTATTCATCAAGAGTGATGCAACTCAAGTCGAGATTAACCCAT
TCGCTGAAACCACCGATGGTCAAGTCATTTGTATGGATGCTAAAATCAATTTCGATGACA
ATGCCTCATTCCGTCAAAAGGAGATTTTCGAAATGAGAGATACCGCCGAAGAGGATCCAC
GTGAGGTTGAAGCTGGTAAATTTGGTCTCAATTACATTGGTCTCGATGGTAACATTGGTT
GTATGGTTAATGGTGCTGGTTTGGCTATGGCCACCATGGATATCATTAAATTAAAGGGTG
GTATCCCTGCTAATTTCCTCGATGTAGGTGGTAGTGCCTCTGAACAAGCCGTCACTGAAG
CCTTCAAAATCCTCACTAAAGATCCAAGAGTTAAATGTTTACTCGTAAACATTTTCGGTG
GTATCATGAAATGTGATATCATTGCCTCTGGTATCGTAAATGCTTCAAAACAAGTTGGTC
TTAAAATTCCATTAGTTGTACGTTTAGAAGGTACAAATGTAAACATTGGTAAAGAAATCT
GGAAAAATCGGTTTAAATATACTTCTGCTAGNATTTAG
Length of connected seq. 868
Full length Seq ID -
Full length Seq. -
Length of full length seq. -