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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFB703
Library VF
(Link to library)
Clone ID VFB703
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U14188-1
Original site URL
Representative seq. ID VFB703Z
(Link to Original site)
Representative DNA sequence 
>VFB703 (VFB703Q) /CSM/VF/VFB7-A/VFB703Q.Seq.d/
XXXXXXXXXXTCATTTTTAGTACAATTGTCAGAGTCAATCTCAGAGTTACCATCATCAGA
TAGATCTATAATCGAATTGAAATTTAGAGATTGGATTAATGAATTGACAAATCTTGATGT
CTCTAGAAATGTTGATATTGCCAATAAAATCAATATTCCACCATCAAAAGAGGGTTTCTT
AAATCCATTGAAAGCATTGCAAATGTTTGATGAACAATTACCACACAAAACCATTATGGT
GGCTGATGGTGGTGATTTCGTTGGTTCTGCAAGTTATATCGTTCGTCCAAGAGCACCATT
ATCTTGGTTGGATCCTGGTGTATTTGGTACATTGGGTGTTGGTGCTGGTTTTTCAATTGC
TGCCAAACTTTGTAGACCTGATCATCAAGTTTGGACAATCTATGGTGATGGTGCTTTCGG
TTATAGTATACCAGAGTTGGATACAATGGTACGTCATAAAATTTCAGTTGGTGCTATCAT
TGGTAATGATTCAGGTTGGATTCAAATCTTACGTGAACAACAAGAACAATTAAATAGTGA
TGTTGGTTGTAATTTAGCTTATACTGATTATCATCAAGTTGCAATAGCATTTGGTGGTAA
AGGTTTTAAATCAACAAATGAATCTGAATTAAAATTTGCTTTAGAAGAATCAAATAAAAT
TTTAAATCAAAATCAACAACCAGTTGTTAT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
---FLVQLSESISELPSSDRSIIELKFRDWINELTNLDVSRNVDIANKINIPPSKEGFLN
PLKALQMFDEQLPHKTIMVADGGDFVGSASYIVRPRAPLSWLDPGVFGTLGVGAGFSIAA
KLCRPDHQVWTIYGDGAFGYSIPELDTMVRHKISVGAIIGNDSGWIQILREQQEQLNSDV
GCNLAYTDYHQVAIAFGGKGFKSTNESELKFALEESNKILNQNQQPVV
Translated Amino Acid sequence (All Frames) 
Frame A:
---hf*yncqsqsqsyhhqidl*sn*nleiglmn*qilmslemlilpiksifhhqkrvs*
ih*khckclmnnyhtkplwwlmvvislvlqvisfvqehhylgwilvylvhwvlvlvfqll
pnfvdliikfgqsmvmvlsvivyqswiqwyvikfqlvlslvmiqvgfksyvnnknn*ivm
lvvi*liliiiklq*hlvvkvlnqqmnln*nll*knqikf*ikinnqll


Frame B:
---ifstivrvnlrvtiir*iynriei*rld**idks*cl*kc*ycq*nqystikrgflk
siesianv**tittqnhygg*ww*frwfcklyrsskstiilvgswciwyigcwcwffncc
qtl*t*sssldnlw*wcfrl*ytrvgyngts*nfswcyhw**frldsnlt*ttrtik**c
wl*fsly*lssscnsiww*rf*ink*i*ikicfrrik*nfksksttscy


Frame C:
---FLVQLSESISELPSSDRSIIELKFRDWINELTNLDVSRNVDIANKINIPPSKEGFLN
PLKALQMFDEQLPHKTIMVADGGDFVGSASYIVRPRAPLSWLDPGVFGTLGVGAGFSIAA
KLCRPDHQVWTIYGDGAFGYSIPELDTMVRHKISVGAIIGNDSGWIQILREQQEQLNSDV
GCNLAYTDYHQVAIAFGGKGFKSTNESELKFALEESNKILNQNQQPVV
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHC522 (VHC522Q) /CSM/VH/VHC5-A/VHC522Q.Seq.d/                       1348   0.0
VFN817 (VFN817Q) /CSM/VF/VFN8-A/VFN817Q.Seq.d/                       1348   0.0
VFB703 (VFB703Q) /CSM/VF/VFB7-A/VFB703Q.Seq.d/                       1348   0.0
VHI317 (VHI317Q) /CSM/VH/VHI3-A/VHI317Q.Seq.d/                       1340   0.0
SFI802 (SFI802Q) /CSM/SF/SFI8-A/SFI802Q.Seq.d/                       1340   0.0
VHC622 (VHC622Q) /CSM/VH/VHC6-A/VHC622Q.Seq.d/                       1334   0.0
VFL161 (VFL161Q) /CSM/VF/VFL1-C/VFL161Q.Seq.d/                       1275   0.0
VHG620 (VHG620Q) /CSM/VH/VHG6-A/VHG620Q.Seq.d/                       1201   0.0
VFD251 (VFD251Q) /CSM/VF/VFD2-C/VFD251Q.Seq.d/                       1134   0.0
VFH285 (VFH285Q) /CSM/VF/VFH2-D/VFH285Q.Seq.d/                       1005   0.0
own update 2002.12.15
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC129593|AC129593.2 Mus musculus chromosome UNK clone RP24-142E6, WORKING DRAFT SEQUENCE, 18 unordered pieces.    36  0.038  5
AC115680|AC115680.2 Dictyostelium discoideum chromosome 2 map 4415041-4520361 strain AX4, complete sequence.    40  0.25  5
AC145741|AC145741.1 Mus musculus chromosome UNK clone RP24-226K1, *** SEQUENCING IN PROGRESS ***, 52 unordered pieces.    46  0.31  5
AC116977|AC116977.2 Dictyostelium discoideum chromosome 2 map 5515173-5817331 strain AX4, complete sequence.    36  0.52  8
AC119032|AC119032.3 Rattus norvegicus clone CH230-206C14, *** SEQUENCING IN PROGRESS ***, 2 unordered pieces.    40  0.53  2
AC141874|AC141874.1 Mus musculus chromosome UNK clone RP24-327L10, WORKING DRAFT SEQUENCE, 60 unordered pieces.    46  0.54  2
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.    34  0.55  10
AC097746|AC097746.7 Rattus norvegicus clone CH230-83I4, *** SEQUENCING IN PROGRESS ***, 8 unordered pieces.    40  0.58  2
AC140401|AC140401.2 Mus musculus chromosome UNK clone RP24-89O8, WORKING DRAFT SEQUENCE, 14 unordered pieces.    46  0.58  1
AC142490|AC142490.1 Mus musculus chromosome UNK clone RP24-362J19, WORKING DRAFT SEQUENCE, 168 unordered pieces.    46  0.58  1
dna update 2003. 9. 6
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BC011761_1(BC011761|pid:none) Homo sapiens ilvB (bacterial aceto...   216   4e-55
(A1L0T0) RecName: Full=Acetolactate synthase-like protein;      ...   216   4e-55
U61263_1(U61263|pid:none) Human acetolactate synthase homolog mR...   216   4e-55
AC004794_3(AC004794|pid:none) Homo sapiens chromosome 19, cosmid...   216   4e-55
BC149989_1(BC149989|pid:none) Bos taurus ilvB (bacterial acetola...   214   3e-54
BT020804_1(BT020804|pid:none) Bos taurus ilvB (bacterial acetola...   214   3e-54
(A6QQT9) RecName: Full=Acetolactate synthase-like protein;      ...   214   3e-54
BC151580_1(BC151580|pid:none) Bos taurus ilvB (bacterial acetola...   214   3e-54
(Q8BU33) RecName: Full=Acetolactate synthase-like protein;      ...   207   3e-52
(Q6NV04) RecName: Full=Acetolactate synthase-like protein;      ...   201   2e-50
protein update 2009. 6.17
PSORT 

 psg: 0.75  gvh: 0.44  alm: 0.38  top: 0.53  tms: 0.00  mit: 0.23  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: cytoplasmic
	 16.0 %: nuclear
	  8.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  8.0 %: vesicles of secretory system
	  4.0 %: Golgi

>> prediction for VFB703 is cyt
5' end seq. ID -
5' end seq. -
Length of 5' end seq. -
3' end seq. ID VFB703Z
3' end seq. 
>VFB703Z.Seq
----------TCATTTTTAGTACAATTGTCAGAGTCAATCTCAGAGTTACCATCATCAGA
TAGATCTATAATCGAATTGAAATTTAGAGATTGGATTAATGAATTGACAAATCTTGATGT
CTCTAGAAATGTTGATATTGCCAATAAAATCAATATTCCACCATCAAAAGAGGGTTTCTT
AAATCCATTGAAAGCATTGCAAATGTTTGATGAACAATTACCACACAAAACCATTATGGT
GGCTGATGGTGGTGATTTCGTTGGTTCTGCAAGTTATATCGTTCGTCCAAGAGCACCATT
ATCTTGGTTGGATCCTGGTGTATTTGGTACATTGGGTGTTGGTGCTGGTTTTTCAATTGC
TGCCAAACTTTGTAGACCTGATCATCAAGTTTGGACAATCTATGGTGATGGTGCTTTCGG
TTATAGTATACCAGAGTTGGATACAATGGTACGTCATAAAATTTCAGTTGGTGCTATCAT
TGGTAATGATTCAGGTTGGATTCAAATCTTACGTGAACAACAAGAACAATTAAATAGTGA
TGTTGGTTGTAATTTAGCTTATACTGATTATCATCAAGTTGCAATAGCATTTGGTGGTAA
AGGTTTTAAATCAACAAATGAATCTGAATTAAAATTTGCTTTAGAAGAATCAAATAAAAT
TTTAAATCAAAATCAACAACCAGTTGTTAT
Length of 3' end seq. 680
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -