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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFB645
Library VF
(Link to library)
Clone ID VFB645
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16382-1
Original site URL
Representative seq. ID VFB645P
(Link to Original site)
Representative DNA sequence 
>VFB645 (VFB645Q) /CSM/VF/VFB6-B/VFB645Q.Seq.d/
AAATATATATATATAAATCATTTAAAAATGGACGGTGAAGATGTTCAAGCTTTAGTTATC
GATAACGGTTCTGGTATGTGTAAAGCCGGTTTTGCTGGTGATGATGCCCCACGTGCTGTT
TTCCCATCAATTGTTGGTCGTCCAAGACACACTGGTGTTATGGTCGGTATGGGTCAAAAA
GACTCATATGTAGGTGATGAAGCCCAATCAAAGAGAGGTATCTTAACCCTCAAATACCCA
ATTGAACACGGTATCGTTACCAACTGGGATGATATGGAAAAAATCTGGCATCATACTTTC
TACAATGAACTCCGTGTTGCCCCAGAAGAACACCCAGTTCTCTTAACTGAAGCCCCATTA
AATCCAAAGGCCAACAGAGAAAAAATGACCCAAATTATGTTTGAAACCTTCAACACCCCA
GCCATGTACGTCGCCATTCAAGCCGTCTTATCCTTATATGCCTCTGGTCGTACCACCGGT
ATTGTTATGGATTCAGGTGATGGTGTCTCCCACACTGTACCAATCTATGAAGGTTATGCT
TTACCACATGCCATCTTACGTTTAGATTTAGCTGGTCGTGATCTTACCGATTACATGATG
AAAATCTTAACTGAACGTGXXXXXXXXXXTCAAGTCATCACCATCGGTAATGAACGTTTC
CGTTGCCCAGAAGCCCTCTTCCAACCATCATTCTTAGGTATGGAATCTGCTGGTATCCAC
GAAACCACCTACAACTCCATCATGAAATGTGATGTTGATATCCGTAAAGATTTATACGGT
AACGTCGTCTTATCCGGTGGTACAACTATGTTCCCAGGTATTGCTGATCGTATGAACAAA
GAATTAACTGCTTTAGCCCCATCAACCATGAAAATTAAAATCATTGCTCCACCAGAACGT
AAATACTCTGTCTGGATTGGTGGATCTATCTTAGCTTCACTCTCAACTTTCCAACAAATG
TGGATCTCCAAAGAAGAATACGACGAATCAGGTCCATCAATTGTTCACAGAAAATGTTTG
CTAAATTATTTAATAAATAATA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
KYIYINHLKMDGEDVQALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQK
DSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPL
NPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVSHTVPIYEGYA
LPHAILRLDLAGRDLTDYMMKILTER---

---QVITIGNERFRCPEALFQPSFLGMESAGIHETTYNSIMKCDVDIRKDLYGNVVLSGG
TTMFPGIADRMNKELTALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKEEY
DESGPSIVHRKCLLNYLINN
Translated Amino Acid sequence (All Frames) 
Frame A:
KYIYINHLKMDGEDVQALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQK
DSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPL
NPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVSHTVPIYEGYA
LPHAILRLDLAGRDLTDYMMKILTER---

---sshhhr**tfplprsplptiilrygicwyprnhlqlhhem*c*yp*rfir*rrlirw
ynyvpryc*syeqrincfspinhen*nhcstrt*ilcldwwiylsftlnfptnvdlqrri
rrirsincsqkmfaklfnk*

Frame B:
niyi*ii*kwtvkmfkl*lsitvlvcvkpvllvmmphvlfshqllvvqdtlvlwsvwvkk
thm*vmkpnqrevs*psntqlntvslptgmiwkksgiilstmnsvlpqkntqfs*lkph*
iqrptekk*pklclkpstpqpctspfkpsypymplvvppvllwiqvmvsptlyqsmkvml
yhmpsyv*i*lvvilpit**ks*lnv---

---QVITIGNERFRCPEALFQPSFLGMESAGIHETTYNSIMKCDVDIRKDLYGNVVLSGG
TTMFPGIADRMNKELTALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKEEY
DESGPSIVHRKCLLNYLINN

Frame C:
iyiyksfkngr*rcssfsyr*rfwyv*srfcw**cptccfpincwsskthwcygrygskr
licr**spikerylnpqipn*tryryqlg*ygknlasyflq*tpccprrtpssln*spik
skgqqrkndpnyv*nlqhpshvrrhssrliliclwsyhrycygfr*wclphctnl*rlcf
ttchltfrfsws*syrlhdenln*t---

---ksspsvmnvsvaqkpssnhhs*vwnllvstkppttps*nvmlisvkiytvtssypvv
qlcsqvlliv*tkn*ll*phqp*klksllhqnvntlsglvdls*lhsqlsnkcgspkknt
tnqvhqlftenvc*ii**ii
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFO588 (VFO588Q) /CSM/VF/VFO5-D/VFO588Q.Seq.d/                       1197   0.0
VFN494 (VFN494Q) /CSM/VF/VFN4-D/VFN494Q.Seq.d/                       1197   0.0
VFM814 (VFM814Q) /CSM/VF/VFM8-A/VFM814Q.Seq.d/                       1197   0.0
VFM550 (VFM550Q) /CSM/VF/VFM5-C/VFM550Q.Seq.d/                       1197   0.0
VFM463 (VFM463Q) /CSM/VF/VFM4-C/VFM463Q.Seq.d/                       1197   0.0
VFL880 (VFL880Q) /CSM/VF/VFL8-D/VFL880Q.Seq.d/                       1197   0.0
VFL749 (VFL749Q) /CSM/VF/VFL7-C/VFL749Q.Seq.d/                       1197   0.0
VFL740 (VFL740Q) /CSM/VF/VFL7-B/VFL740Q.Seq.d/                       1197   0.0
VFL458 (VFL458Q) /CSM/VF/VFL4-C/VFL458Q.Seq.d/                       1197   0.0
VFL313 (VFL313Q) /CSM/VF/VFL3-A/VFL313Q.Seq.d/                       1197   0.0
own update 2004. 8.27
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

X03281|X03281.1 Dictyostelium discoideum gene for actin A8.     1174  0.0  2
AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.  1100  0.0  4
M14146|M14146.1 D.discoideum actin 15 gene, complete cds.       1088  0.0  2
AC115579|AC115579.2 Dictyostelium discoideum chromosome 2 map 4915084-5005461 strain AX4, complete sequence.  1088  0.0  3
U25660|U25660.1 Dictyostelium discoideum actin gene, partial cds.  1057  0.0  2
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.   981  0.0  6
AC115577|AC115577.2 Dictyostelium discoideum chromosome 2 map 4657875-4914984 strain AX4, complete sequence.   938  0.0  12
AC116956|AC116956.2 Dictyostelium discoideum chromosome 2 map 1418423-1684967 strain AX4, complete sequence.   910  0.0  6
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.   864  0.0  3
X03282|X03282.1 dictyostelium discoideum gene for actin A12.     856  0.0  2
dna update 2003. 9. 5
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54H30) RecName: Full=Major actin; AltName: Full=Actin-1; AltNa...   640   0.0
ATDO(A93223;A92871;S67999;A03004)actin - slime mold (Dictyosteli...   639   0.0
(Q553U6) RecName: Full=Putative actin-22;  &AC116305_3(AC116305|...   637   0.0
(P02576) RecName: Full=Actin, plasmodial isoform;  &ATFY(A29044;...   635   0.0
B23412(B23412)actin 12 - slime mold (Dictyostelium discoideum)        634   e-180
X03282_1(X03282|pid:none) dictyostelium discoideum gene for acti...   633   e-180
(P07829) RecName: Full=Actin-3; AltName: Full=Actin-3-sub 1;          625   e-177
(P02578) RecName: Full=Actin-1;  &ATAX(A92886;A03004) &V00002_1(...   624   e-177
AY294154_1(AY294154|pid:none) Thecamoeba similis actin mRNA, com...   624   e-177
AF082863_1(AF082863|pid:none) Dreissena polymorpha cytoplasmic a...   617   e-175
protein update 2009. 6.17
PSORT 

 psg: 0.64  gvh: 0.34  alm: 0.42  top: 0.53  tms: 0.00  mit: 0.17  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 40.0 %: nuclear
	 12.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for VFB645 is cyt
5' end seq. ID VFB645F
5' end seq. 
>VFB645F.Seq
AAATATATATATATAAATCATTTAAAAATGGACGGTGAAGATGTTCAAGCTTTAGTTATC
GATAACGGTTCTGGTATGTGTAAAGCCGGTTTTGCTGGTGATGATGCCCCACGTGCTGTT
TTCCCATCAATTGTTGGTCGTCCAAGACACACTGGTGTTATGGTCGGTATGGGTCAAAAA
GACTCATATGTAGGTGATGAAGCCCAATCAAAGAGAGGTATCTTAACCCTCAAATACCCA
ATTGAACACGGTATCGTTACCAACTGGGATGATATGGAAAAAATCTGGCATCATACTTTC
TACAATGAACTCCGTGTTGCCCCAGAAGAACACCCAGTTCTCTTAACTGAAGCCCCATTA
AATCCAAAGGCCAACAGAGAAAAAATGACCCAAATTATGTTTGAAACCTTCAACACCCCA
GCCATGTACGTCGCCATTCAAGCCGTCTTATCCTTATATGCCTCTGGTCGTACCACCGGT
ATTGTTATGGATTCAGGTGATGGTGTCTCCCACACTGTACCAATCTATGAAGGTTATGCT
TTACCACATGCCATCTTACGTTTAGATTTAGCTGGTCGTGATCTTACCGATTACATGATG
AAAATCTTAACTGAACGTG----------
Length of 5' end seq. 619
3' end seq. ID VFB645Z
3' end seq. 
>VFB645Z.Seq
----------TCAAGTCATCACCATCGGTAATGAACGTTTCCGTTGCCCAGAAGCCCTCT
TCCAACCATCATTCTTAGGTATGGAATCTGCTGGTATCCACGAAACCACCTACAACTCCA
TCATGAAATGTGATGTTGATATCCGTAAAGATTTATACGGTAACGTCGTCTTATCCGGTG
GTACAACTATGTTCCCAGGTATTGCTGATCGTATGAACAAAGAATTAACTGCTTTAGCCC
CATCAACCATGAAAATTAAAATCATTGCTCCACCAGAACGTAAATACTCTGTCTGGATTG
GTGGATCTATCTTAGCTTCACTCTCAACTTTCCAACAAATGTGGATCTCCAAAGAAGAAT
ACGACGAATCAGGTCCATCAATTGTTCACAGAAAATGTTTGCTAAATTATTTAATAAATA
ATA
Length of 3' end seq. 413
Connected seq. ID VFB645P
Connected seq. 
>VFB645P.Seq
AAATATATATATATAAATCATTTAAAAATGGACGGTGAAGATGTTCAAGCTTTAGTTATC
GATAACGGTTCTGGTATGTGTAAAGCCGGTTTTGCTGGTGATGATGCCCCACGTGCTGTT
TTCCCATCAATTGTTGGTCGTCCAAGACACACTGGTGTTATGGTCGGTATGGGTCAAAAA
GACTCATATGTAGGTGATGAAGCCCAATCAAAGAGAGGTATCTTAACCCTCAAATACCCA
ATTGAACACGGTATCGTTACCAACTGGGATGATATGGAAAAAATCTGGCATCATACTTTC
TACAATGAACTCCGTGTTGCCCCAGAAGAACACCCAGTTCTCTTAACTGAAGCCCCATTA
AATCCAAAGGCCAACAGAGAAAAAATGACCCAAATTATGTTTGAAACCTTCAACACCCCA
GCCATGTACGTCGCCATTCAAGCCGTCTTATCCTTATATGCCTCTGGTCGTACCACCGGT
ATTGTTATGGATTCAGGTGATGGTGTCTCCCACACTGTACCAATCTATGAAGGTTATGCT
TTACCACATGCCATCTTACGTTTAGATTTAGCTGGTCGTGATCTTACCGATTACATGATG
AAAATCTTAACTGAACGTG----------TCAAGTCATCACCATCGGTAATGAACGTTTC
CGTTGCCCAGAAGCCCTCTTCCAACCATCATTCTTAGGTATGGAATCTGCTGGTATCCAC
GAAACCACCTACAACTCCATCATGAAATGTGATGTTGATATCCGTAAAGATTTATACGGT
AACGTCGTCTTATCCGGTGGTACAACTATGTTCCCAGGTATTGCTGATCGTATGAACAAA
GAATTAACTGCTTTAGCCCCATCAACCATGAAAATTAAAATCATTGCTCCACCAGAACGT
AAATACTCTGTCTGGATTGGTGGATCTATCTTAGCTTCACTCTCAACTTTCCAACAAATG
TGGATCTCCAAAGAAGAATACGACGAATCAGGTCCATCAATTGTTCACAGAAAATGTTTG
CTAAATTATTTAATAAATAATA
Length of connected seq. 1032
Full length Seq ID -
Full length Seq. -
Length of full length seq. -