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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFB625
Library VF
(Link to library)
Clone ID VFB625
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U13825-1
Original site URL
Representative seq. ID VFB625P
(Link to Original site)
Representative DNA sequence 
>VFB625 (VFB625Q) /CSM/VF/VFB6-B/VFB625Q.Seq.d/
ACTGTTGGCCTACTGGCAAAAATGTTAAGAATTAACAGAATTTCTAACTTACGTACTTTT
GGTCAACGTTTCTTCTCAACTGAACAACAAGATGTTGTTGTTATTGGTGGTGGACCAGGT
GGTTATGTTGCAGGTATTAAAGCAGGTCAATTAGGTATGAAAGTTACAGTTGTAGAGAAA
AGAGGTAAATTAGGAGGAACATGTTTAAATGTTGGTTGTATTCCATCAAAAGCATTATTG
AATGCATCACATCTTTATGAAGAAGCCACCACTAGAATGTCCAAATATGGTGTCAAATGT
TCAGGTGTTGAATTAGATTTGGGAGCAATGATGCAATACAAAGATAAATCAGTCAGTGGC
TTAACATCAGGTATTGAAGGTTTATTCAAAAAGAACAAAGTAAAATATGATAAAGGTTTC
GGTAAAATCACTGGCCCAAACACTGTCGAAGTTACATTAAATGACNGTTCCGTTAAAACT
ATCGAAACCAAAXXXXXXXXXXTGAAATTCCATCTCGAAACTAAAGTTACCTCTGTCGTT
AAAAAATCTGATGGTAAGGTTACTGTAACCGTTGAACAAGTTGGTGCTGGTGGTTTCACT
GGTACTCTCGAAGCTGATGCTGTCCTTGTCTCTGTTGGTCGTCGTCCAAATACTAGTGGT
CTTGGTCTTGAATCAGTTGGTATCCCAACCGATAAAGCTGGTCGTGTCGAAGTTGGAGAT
CACTTTAACACTAAAGTTCCATCAATTTTCGCAATTGGTGATGCCATCCGTGGTCCAATG
CTTGCTCACAAAGCTGAAGAAGAAGGTATTGCTATCATTGAACAAATTCACAATGGTGGT
GGTCATGTTAACTATGGTGCCATCCCATCCATTATCTACACTCACCCAGAAGTTGCTTGG
GTTGGTAAGACTGAAGAAGAATTACAAAAAGAAGGTATCCAATACAATATTGGTAGATTC
CCATTCGTTGCAAACTCTAGAGCCAAAACAAACGATGATGTCGAAGGTTTCGTTAAATTC
CTCGCCGCTAAAGATTCTGATAGAGTCCTTGGTGCTCATATTATGGGTACCAATGCTGGT
GAATTAATTGGTGAATGTGTTTTAGCTATGGAATATGGTGCCTCATGTGAAGATATTGCA
AGAACTTGTCATGGTCATCCAACTTTATCCGAAGCTGTTAAAGAAGC
sequence update 2001. 6. 1
Translated Amino Acid sequence 
TVGLLAKMLRINRISNLRTFGQRFFSTEQQDVVVIGGGPGGYVAGIKAGQLGMKVTVVEK
RGKLGGTCLNVGCIPSKALLNASHLYEEATTRMSKYGVKCSGVELDLGAMMQYKDKSVSG
LTSGIEGLFKKNKVKYDKGFGKITGPNTVEVTLNDXSVKTIETK---

---KFHLETKVTSVVKKSDGKVTVTVEQVGAGGFTGTLEADAVLVSVGRRPNTSGLGLES
VGIPTDKAGRVEVGDHFNTKVPSIFAIGDAIRGPMLAHKAEEEGIAIIEQIHNGGGHVNY
GAIPSIIYTHPEVAWVGKTEEELQKEGIQYNIGRFPFVANSRAKTNDDVEGFVKFLAAKD
SDRVLGAHIMGTNAGELIGECVLAMEYGASCEDIARTCHGHPTLSEAVKE
Translated Amino Acid sequence (All Frames) 
Frame A:
TVGLLAKMLRINRISNLRTFGQRFFSTEQQDVVVIGGGPGGYVAGIKAGQLGMKVTVVEK
RGKLGGTCLNVGCIPSKALLNASHLYEEATTRMSKYGVKCSGVELDLGAMMQYKDKSVSG
LTSGIEGLFKKNKVKYDKGFGKITGPNTVEVTLNDXSVKTIETK---

---*nsisklklplslknlmvrll*plnklvlvvslvlsklmlslsllvvvqilvvlvln
qlvsqpiklvvskleitltlkfhqfsqlvmpsvvqclltklkkkvllslnkftmvvvmlt
mvpshplstltqkllglvrlkknykkkvsntilvdshslqtlepkqtmmskvslnssplk
ilieslvlilwvpmlvn*lvnvf*lwnmvphvkilqelvmviqlypkllkk

Frame B:
llaywqkc*eltefltyvllvnvssqlnnkmllllvvdqvvmlqvlkqvn*v*klql*rk
evn*eehv*mlvvfhqkhy*mhhifmkkpplecpnmvsnvqvln*iweq*cntkinqsva
*hqvlkvyskrtk*nmikvsvkslaqtlsklh*mtvplklskp---

---eipsrn*sylcr*ki*w*gycnr*tswcwwfhwysrs*ccpclcwsssky*wsws*i
swypnr*swscrswrsl*h*ssinfrnw*chpwsnacsqs*rrrycyh*tnsqwwwsc*l
wchpihylhsprsclgw*d*rritkrrypiqyw*ipirckl*sqnkr*crrfr*iprr*r
f**spwcsyygyqcw*inw*mcfsygiwclm*rycknlswssnfirsc*rs

Frame C:
cwptgknvkn*qnf*ltyfwstflln*ttrcccywwwtrwlccry*srsiryesyscrek
r*irrnmfkcwlysiksiiecitsl*rshh*nvqiwcqmfrc*irfgsndaiqr*isqwl
niry*rfiqkeqski**rfr*nhwpkhcrsyik*xfr*nyrnq---

---KFHLETKVTSVVKKSDGKVTVTVEQVGAGGFTGTLEADAVLVSVGRRPNTSGLGLES
VGIPTDKAGRVEVGDHFNTKVPSIFAIGDAIRGPMLAHKAEEEGIAIIEQIHNGGGHVNY
GAIPSIIYTHPEVAWVGKTEEELQKEGIQYNIGRFPFVANSRAKTNDDVEGFVKFLAAKD
SDRVLGAHIMGTNAGELIGECVLAMEYGASCEDIARTCHGHPTLSEAVKE
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFB625 (VFB625Q) /CSM/VF/VFB6-B/VFB625Q.Seq.d/                       2288   0.0
AFB294 (AFB294Q) /CSM/AF/AFB2-D/AFB294Q.Seq.d/                       1358   0.0
VFC622 (VFC622Q) /CSM/VF/VFC6-A/VFC622Q.Seq.d/                       1344   0.0
AFD276 (AFD276Q) /CSM/AF/AFD2-D/AFD276Q.Seq.d/                       1344   0.0
AFC230 (AFC230Q) /CSM/AF/AFC2-B/AFC230Q.Seq.d/                       1344   0.0
VFB687 (VFB687Q) /CSM/VF/VFB6-D/VFB687Q.Seq.d/                       1342   0.0
CFG142 (CFG142Q) /CSM/CF/CFG1-B/CFG142Q.Seq.d/                       1342   0.0
VFE339 (VFE339Q) /CSM/VF/VFE3-B/VFE339Q.Seq.d/                       1328   0.0
CFF221 (CFF221Q) /CSM/CF/CFF2-A/CFF221Q.Seq.d/                       1328   0.0
VFK250 (VFK250Q) /CSM/VF/VFK2-C/VFK250Q.Seq.d/                       1320   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AX489387|AX489387.1 Sequence 6687 from Patent WO02053728.         56  3e-37  6
AR180935|AR180935.1 Sequence 7 from patent US 6333164.            56  2e-32  6
AL423544|AL423544.1 T3 end of clone AZ0AA009B01 of library AZ0AA from strain CBS 712 of Kluyveromyces marxianus.    84  1e-23  4
BZ296408|BZ296408.1 CG2037.f11 Candida glabrata Random Genomic Library Candida glabrata genomic clone CG2037, DNA sequence.    64  7e-23  4
AL428740|AL428740.1 clone BA0AB027E08 of library BA0AB from strain CLIB 210 of  Kluyveromyces lactis.    70  1e-20  3
AL439032|AL439032.1 T3 end of clone BD0AA002A06 of library BD0AA from strain CBS 94 of  Candida tropicalis.    54  3e-19  5
J03489|J03489.1 Pig lipoamide dehydrogenase mRNA, complete cds.    42  3e-15  5
J03620|J03620.1 Human dihydrolipoamide dehydrogenase mRNA, complete cds.    46  4e-15  4
BC018696|BC018696.2 Homo sapiens dihydrolipoamide dehydrogenase (E3 component of pyruvate dehydrogenase complex, 2-oxo-glutarate complex, branched chain keto acid dehydrogenase complex), mRNA (cDNA clone MGC:3866 IMAGE:3029752), complete cds.    46  4e-15  4
BC018648|BC018648.2 Homo sapiens dihydrolipoamide dehydrogenase (E3 component of pyruvate dehydrogenase complex, 2-oxo-glutarate complex, branched chain keto acid dehydrogenase complex), mRNA (cDNA clone MGC:2115 IMAGE:3029752), complete cds.    46  4e-15  4
dna update 2003. 9. 5
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY391421_1(AY391421|pid:none) Danio rerio dihydrolipoamide dehyd...   278   4e-73
AK294146_1(AK294146|pid:none) Homo sapiens cDNA FLJ56061 complet...   274   5e-72
AK297508_1(AK297508|pid:none) Homo sapiens cDNA FLJ56112 complet...   274   5e-72
AK295080_1(AK295080|pid:none) Homo sapiens cDNA FLJ50515 complet...   274   5e-72
(P09622) RecName: Full=Dihydrolipoyl dehydrogenase, mitochondria...   274   5e-72
AK312346_1(AK312346|pid:none) Homo sapiens cDNA, FLJ92665, highl...   274   5e-72
(O08749) RecName: Full=Dihydrolipoyl dehydrogenase, mitochondria...   273   7e-72
AK153399_1(AK153399|pid:none) Mus musculus bone marrow macrophag...   273   7e-72
AK136193_1(AK136193|pid:none) Mus musculus in vitro fertilized e...   273   7e-72
U73445_1(U73445|pid:none) Mus musculus dihydrolipoamide dehydrog...   273   7e-72
protein update 2009. 6.17
PSORT 

 psg: 0.50  gvh: 0.36  alm: 0.42  top: 0.53  tms: 0.00  mit: 0.45  mip: 0.05
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: mitochondrial
	 32.0 %: cytoplasmic
	 16.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: plasma membrane

>> prediction for VFB625 is mit
5' end seq. ID VFB625F
5' end seq. 
>VFB625F.Seq
ACTGTTGGCCTACTGGCAAAAATGTTAAGAATTAACAGAATTTCTAACTTACGTACTTTT
GGTCAACGTTTCTTCTCAACTGAACAACAAGATGTTGTTGTTATTGGTGGTGGACCAGGT
GGTTATGTTGCAGGTATTAAAGCAGGTCAATTAGGTATGAAAGTTACAGTTGTAGAGAAA
AGAGGTAAATTAGGAGGAACATGTTTAAATGTTGGTTGTATTCCATCAAAAGCATTATTG
AATGCATCACATCTTTATGAAGAAGCCACCACTAGAATGTCCAAATATGGTGTCAAATGT
TCAGGTGTTGAATTAGATTTGGGAGCAATGATGCAATACAAAGATAAATCAGTCAGTGGC
TTAACATCAGGTATTGAAGGTTTATTCAAAAAGAACAAAGTAAAATATGATAAAGGTTTC
GGTAAAATCACTGGCCCAAACACTGTCGAAGTTACATTAAATGACNGTTCCGTTAAAACT
ATCGAAACCAAA----------
Length of 5' end seq. 492
3' end seq. ID VFB625Z
3' end seq. 
>VFB625Z.Seq
----------TGAAATTCCATCTCGAAACTAAAGTTACCTCTGTCGTTAAAAAATCTGAT
GGTAAGGTTACTGTAACCGTTGAACAAGTTGGTGCTGGTGGTTTCACTGGTACTCTCGAA
GCTGATGCTGTCCTTGTCTCTGTTGGTCGTCGTCCAAATACTAGTGGTCTTGGTCTTGAA
TCAGTTGGTATCCCAACCGATAAAGCTGGTCGTGTCGAAGTTGGAGATCACTTTAACACT
AAAGTTCCATCAATTTTCGCAATTGGTGATGCCATCCGTGGTCCAATGCTTGCTCACAAA
GCTGAAGAAGAAGGTATTGCTATCATTGAACAAATTCACAATGGTGGTGGTCATGTTAAC
TATGGTGCCATCCCATCCATTATCTACACTCACCCAGAAGTTGCTTGGGTTGGTAAGACT
GAAGAAGAATTACAAAAAGAAGGTATCCAATACAATATTGGTAGATTCCCATTCGTTGCA
AACTCTAGAGCCAAAACAAACGATGATGTCGAAGGTTTCGTTAAATTCCTCGCCGCTAAA
GATTCTGATAGAGTCCTTGGTGCTCATATTATGGGTACCAATGCTGGTGAATTAATTGGT
GAATGTGTTTTAGCTATGGAATATGGTGCCTCATGTGAAGATATTGCAAGAACTTGTCAT
GGTCATCCAACTTTATCCGAAGCTGTTAAAGAAGC
Length of 3' end seq. 685
Connected seq. ID VFB625P
Connected seq. 
>VFB625P.Seq
ACTGTTGGCCTACTGGCAAAAATGTTAAGAATTAACAGAATTTCTAACTTACGTACTTTT
GGTCAACGTTTCTTCTCAACTGAACAACAAGATGTTGTTGTTATTGGTGGTGGACCAGGT
GGTTATGTTGCAGGTATTAAAGCAGGTCAATTAGGTATGAAAGTTACAGTTGTAGAGAAA
AGAGGTAAATTAGGAGGAACATGTTTAAATGTTGGTTGTATTCCATCAAAAGCATTATTG
AATGCATCACATCTTTATGAAGAAGCCACCACTAGAATGTCCAAATATGGTGTCAAATGT
TCAGGTGTTGAATTAGATTTGGGAGCAATGATGCAATACAAAGATAAATCAGTCAGTGGC
TTAACATCAGGTATTGAAGGTTTATTCAAAAAGAACAAAGTAAAATATGATAAAGGTTTC
GGTAAAATCACTGGCCCAAACACTGTCGAAGTTACATTAAATGACNGTTCCGTTAAAACT
ATCGAAACCAAA----------TGAAATTCCATCTCGAAACTAAAGTTACCTCTGTCGTT
AAAAAATCTGATGGTAAGGTTACTGTAACCGTTGAACAAGTTGGTGCTGGTGGTTTCACT
GGTACTCTCGAAGCTGATGCTGTCCTTGTCTCTGTTGGTCGTCGTCCAAATACTAGTGGT
CTTGGTCTTGAATCAGTTGGTATCCCAACCGATAAAGCTGGTCGTGTCGAAGTTGGAGAT
CACTTTAACACTAAAGTTCCATCAATTTTCGCAATTGGTGATGCCATCCGTGGTCCAATG
CTTGCTCACAAAGCTGAAGAAGAAGGTATTGCTATCATTGAACAAATTCACAATGGTGGT
GGTCATGTTAACTATGGTGCCATCCCATCCATTATCTACACTCACCCAGAAGTTGCTTGG
GTTGGTAAGACTGAAGAAGAATTACAAAAAGAAGGTATCCAATACAATATTGGTAGATTC
CCATTCGTTGCAAACTCTAGAGCCAAAACAAACGATGATGTCGAAGGTTTCGTTAAATTC
CTCGCCGCTAAAGATTCTGATAGAGTCCTTGGTGCTCATATTATGGGTACCAATGCTGGT
GAATTAATTGGTGAATGTGTTTTAGCTATGGAATATGGTGCCTCATGTGAAGATATTGCA
AGAACTTGTCATGGTCATCCAACTTTATCCGAAGCTGTTAAAGAAGC
Length of connected seq. 1177
Full length Seq ID -
Full length Seq. -
Length of full length seq. -