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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFB606
Library VF
(Link to library)
Clone ID VFB606
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16382-1
Original site URL
Representative seq. ID VFB606E
(Link to Original site)
Representative DNA sequence 
>VFB606 (VFB606Q) /CSM/VF/VFB6-A/VFB606Q.Seq.d/
AATACTTAATTCAAATATATATATATAAATCATTTAAAAATGGACGGTGAAGATGTTCAA
GCTTTAGTTATCGATAACGGTTCTGGTATGTGTAAAGCCGGTTTTGCTGGTGATGATGCC
CCACGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGACACACTGGTGTTATGGTCGGT
ATGGGTCAAAAAGACTCATATGTAGGTGATGAAGCCCAATCAAAGAGAGGTATCTTAACC
CTCAAATACCCAATTGAACACGGTATCGTTACCAACTGGGATGATATGGAAAAAATCTGG
CATCATACTTTCTACAATGAACTCCGTGTTGCCCCAGAAGAACACCCAGTTCTCTTAACT
GAAGCCCCATTAAATCCAAAGGCCAACAGAGAAAAAATGACCCAAATTATGTTTGAAACC
TTCAACACCCCAGCCATGTACGTCGCCATTCAAGCCGTCTTATCCTTATATGCCTCTGGT
CGTACCACCGGTATTGTTATGGATTCAGGTGATGGTGTCTCCCACACTGTACCAATCTAT
GAAGGTTANGCTTTACCACATGCCATCTTACGTTTAGATTTAGCTGGTCGTGATCTTACC
GATTACATGATGAAAATCTTAACTGAACGTGGTTACTCATTCACCACCACTGCCGAAAGA
GAAATCGTCAGAGATATCAAAGAAGAATTAGCCTACGTCGCCCTCGACTTTGAAGCTGAA
ATGCAAACTGCTGCCTCATCATCAGCCCTCGAAAAATCATACGAATTACCAGACGGTCAA
GTCATCACCATCGGTAATGAACGTTTCCGTTGCCCAGAAGCCCTCTTCCAACCATCATTC
TTAGGTATGGAATCTGCTGGTATCCACGAAACCACCTACAACTCCATCATGAAATGTGAT
GTTGATATCCGTAAAGATTTATACGGTAACGTCGTCTTATCCGGTGGTACAACTATGTTC
CCAGGTATTGCTGATCGTATGAACAAAGAATTAACTGCTTTAGCCCCATCAACCATGAAA
ATTAAAATCATTGCTCCACCAGAACGTAAATACTCTGTCTGGATTGGTGGATCTATCTTA
GCTTCACTCTCAACTTTCCAACAAATGTGGATCTCCAAAGAAGAATACGACGAATCAGGT
CCATCAATTGTTCACAGAAAATGTTTCTAAATTATTTAATAAATAATAAAAAAACAAAT
sequence update 2001. 6. 9
Translated Amino Acid sequence 
nt*FKYIYINHLKMDGEDVQALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHTGVMVG
MGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLT
EAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVSHTVPIY
EGXALPHAILRLDLAGRDLTDYMMKILTERGYSFTTTAEREIVRDIKEELAYVALDFEAE
MQTAASSSALEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESAGIHETTYNSIMKCD
VDIRKDLYGNVVLSGGTTMFPGIADRMNKELTALAPSTMKIKIIAPPERKYSVWIGGSIL
ASLSTFQQMWISKEEYDESGPSIVHRKCF*ii**iikkq
Translated Amino Acid sequence (All Frames) 
Frame A:
nt*FKYIYINHLKMDGEDVQALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHTGVMVG
MGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLT
EAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVSHTVPIY
EGXALPHAILRLDLAGRDLTDYMMKILTERGYSFTTTAEREIVRDIKEELAYVALDFEAE
MQTAASSSALEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESAGIHETTYNSIMKCD
VDIRKDLYGNVVLSGGTTMFPGIADRMNKELTALAPSTMKIKIIAPPERKYSVWIGGSIL
ASLSTFQQMWISKEEYDESGPSIVHRKCF*ii**iikkq


Frame B:
ilnsniyi*ii*kwtvkmfkl*lsitvlvcvkpvllvmmphvlfshqllvvqdtlvlwsv
wvkkthm*vmkpnqrevs*psntqlntvslptgmiwkksgiilstmnsvlpqkntqfs*l
kph*iqrptekk*pklclkpstpqpctspfkpsypymplvvppvllwiqvmvsptlyqsm
kvxlyhmpsyv*i*lvvilpit**ks*lnvvthspplpkeksseiskkn*ptspstlklk
ckllphhqpsknhtnyqtvksspsvmnvsvaqkpssnhhs*vwnllvstkppttps*nvm
lisvkiytvtssypvvqlcsqvlliv*tkn*ll*phqp*klksllhqnvntlsglvdls*
lhsqlsnkcgspkknttnqvhqlftenvsklfnk**knk


Frame C:
yliqiyiyksfkngr*rcssfsyr*rfwyv*srfcw**cptccfpincwsskthwcygry
gskrlicr**spikerylnpqipn*tryryqlg*ygknlasyflq*tpccprrtpssln*
spikskgqqrkndpnyv*nlqhpshvrrhssrliliclwsyhrycygfr*wclphctnl*
rlxfttchltfrfsws*syrlhdenln*twllihhhcrkrnrqryqrrislrrprl*s*n
anccliisprkiiritrrsshhhr**tfplprsplptiilrygicwyprnhlqlhhem*c
*yp*rfir*rrlirwynyvpryc*syeqrincfspinhen*nhcstrt*ilcldwwiyls
ftlnfptnvdlqrrirrirsincsqkmflnylinnkktn
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFB606 (VFB606Q) /CSM/VF/VFB6-A/VFB606Q.Seq.d/                       2294   0.0
VFM550 (VFM550Q) /CSM/VF/VFM5-C/VFM550Q.Seq.d/                       2286   0.0
VFL880 (VFL880Q) /CSM/VF/VFL8-D/VFL880Q.Seq.d/                       2286   0.0
VFI577 (VFI577Q) /CSM/VF/VFI5-D/VFI577Q.Seq.d/                       2286   0.0
VFI526 (VFI526Q) /CSM/VF/VFI5-B/VFI526Q.Seq.d/                       2286   0.0
VFI515 (VFI515Q) /CSM/VF/VFI5-A/VFI515Q.Seq.d/                       2286   0.0
VFH672 (VFH672Q) /CSM/VF/VFH6-C/VFH672Q.Seq.d/                       2286   0.0
VFH578 (VFH578Q) /CSM/VF/VFH5-D/VFH578Q.Seq.d/                       2286   0.0
VFE572 (VFE572Q) /CSM/VF/VFE5-C/VFE572Q.Seq.d/                       2286   0.0
VFD711 (VFD711Q) /CSM/VF/VFD7-A/VFD711Q.Seq.d/                       2286   0.0
own update 2001.12. 4
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

X03281|X03281.1 Dictyostelium discoideum gene for actin A8.     2228  0.0  1
AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.  2101  0.0  3
M14146|M14146.1 D.discoideum actin 15 gene, complete cds.       2028  0.0  1
AC115579|AC115579.2 Dictyostelium discoideum chromosome 2 map 4915084-5005461 strain AX4, complete sequence.  2028  0.0  2
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.  1840  0.0  3
AC115577|AC115577.2 Dictyostelium discoideum chromosome 2 map 4657875-4914984 strain AX4, complete sequence.  1752  0.0  14
AC116956|AC116956.2 Dictyostelium discoideum chromosome 2 map 1418423-1684967 strain AX4, complete sequence.  1665  0.0  7
X03282|X03282.1 dictyostelium discoideum gene for actin A12.    1610  0.0  1
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.  1493  0.0  2
U25660|U25660.1 Dictyostelium discoideum actin gene, partial cds.  1366  0.0  1
dna update 2003. 9. 5
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54H30) RecName: Full=Major actin; AltName: Full=Actin-1; AltNa...   754   0.0
(Q553U6) RecName: Full=Putative actin-22;  &AC116305_3(AC116305|...   749   0.0
ATDO(A93223;A92871;S67999;A03004)actin - slime mold (Dictyosteli...   748   0.0
B23412(B23412)actin 12 - slime mold (Dictyostelium discoideum)        748   0.0
(P02576) RecName: Full=Actin, plasmodial isoform;  &ATFY(A29044;...   747   0.0
X03282_1(X03282|pid:none) dictyostelium discoideum gene for acti...   746   0.0
(P07829) RecName: Full=Actin-3; AltName: Full=Actin-3-sub 1;          737   0.0
AY294154_1(AY294154|pid:none) Thecamoeba similis actin mRNA, com...   734   0.0
(P02578) RecName: Full=Actin-1;  &ATAX(A92886;A03004) &V00002_1(...   732   0.0
AF082863_1(AF082863|pid:none) Dreissena polymorpha cytoplasmic a...   726   0.0
protein update 2009. 7.10
PSORT 

 psg: 0.53  gvh: 0.34  alm: 0.42  top: 0.53  tms: 0.00  mit: 0.17  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: cytoplasmic
	 28.0 %: nuclear
	  8.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: peroxisomal

>> prediction for VFB606 is cyt
5' end seq. ID VFB606F
5' end seq. 
>VFB606F.Seq
AATACTTAATTCAAATATATATATATAAATCATTTAAAAATGGACGGTGAAGATGTTCAA
GCTTTAGTTATCGATAACGGTTCTGGTATGTGTAAAGCCGGTTTTGCTGGTGATGATGCC
CCACGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGACACACTGGTGTTATGGTCGGT
ATGGGTCAAAAAGACTCATATGTAGGTGATGAAGCCCAATCAAAGAGAGGTATCTTAACC
CTCAAATACCCAATTGAACACGGTATCGTTACCAACTGGGATGATATGGAAAAAATCTGG
CATCATACTTTCTACAATGAACTCCGTGTTGCCCCAGAAGAACACCCAGTTCTCTTAACT
GAAGCCCCATTAAATCCAAAGGCCAACAGAGAAAAAATGACCCAAATTATGTTTGAAACC
TTCAACACCCCAGCCATGTACGTCGCCATTCAAGCCGTCTTATCCTTATATGCCTCTGGT
CGTACCACCGGTATTGTTATGGATTCAGGTGATGGTGTCTCCCACACTGTACCAATCTAT
GAAGGTTATGCTTTACCACATGCCATCTTACGTTTAGATTTAGCTGGTCGTGATCTTACC
GATTACATGATGAAA----------
Length of 5' end seq. 615
3' end seq. ID VFB606Z
3' end seq. 
>VFB606Z.Seq
----------TCTATGAAGGTTAGGCTTTACCACATGCCATCTTACGTTTAGATTTAGCT
GGTCGTGATCTTACCGATTACATGATGAAAATCTTAACTGAACGTGGTTACTCATTCACC
ACCACTGCCGAAAGAGAAATCGTCAGAGATATCAAAGAAGAATTAGCCTACGTCGCCCTC
GACTTTGAAGCTGAAATGCAAACTGCTGCCTCATCATCAGCCCTCGAAAAATCATACGAA
TTACCAGACGGTCAAGTCATCACCATCGGTAATGAACGTTTCCGTTGCCCAGAAGCCCTC
TTCCAACCATCATTCTTAGGTATGGAATCTGCTGGTATCCACGAAACCACCTACAACTCC
ATCATGAAATGTGATGTTGATATCCGTAAAGATTTATACGGTAACGTCGTCTTATCCGGT
GGTACAACTATGTTCCCAGGTATTGCTGATCGTATGAACAAAGAATTAACTGCTTTAGCC
CCATCAACCATGAAAATTAAAATCATTGCTCCACCAGAACGTAAATACTCTGTCTGGATT
GGTGGATCTATCTTAGCTTCACTCTCAACTTTCCAACAAATGTGGATCTCCAAAGAAGAA
TACGACGAATCAGGTCCATCAATTGTTCACAGAAAATGTTTCTAAATTATTTAATAAATA
ATAAAAAAACAAAT
Length of 3' end seq. 664
Connected seq. ID VFB606P
Connected seq. 
>VFB606P.Seq
AATACTTAATTCAAATATATATATATAAATCATTTAAAAATGGACGGTGAAGATGTTCAA
GCTTTAGTTATCGATAACGGTTCTGGTATGTGTAAAGCCGGTTTTGCTGGTGATGATGCC
CCACGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGACACACTGGTGTTATGGTCGGT
ATGGGTCAAAAAGACTCATATGTAGGTGATGAAGCCCAATCAAAGAGAGGTATCTTAACC
CTCAAATACCCAATTGAACACGGTATCGTTACCAACTGGGATGATATGGAAAAAATCTGG
CATCATACTTTCTACAATGAACTCCGTGTTGCCCCAGAAGAACACCCAGTTCTCTTAACT
GAAGCCCCATTAAATCCAAAGGCCAACAGAGAAAAAATGACCCAAATTATGTTTGAAACC
TTCAACACCCCAGCCATGTACGTCGCCATTCAAGCCGTCTTATCCTTATATGCCTCTGGT
CGTACCACCGGTATTGTTATGGATTCAGGTGATGGTGTCTCCCACACTGTACCAATCTAT
GAAGGTTATGCTTTACCACATGCCATCTTACGTTTAGATTTAGCTGGTCGTGATCTTACC
GATTACATGATGAAA----------TCTATGAAGGTTAGGCTTTACCACATGCCATCTTA
CGTTTAGATTTAGCTGGTCGTGATCTTACCGATTACATGATGAAAATCTTAACTGAACGT
GGTTACTCATTCACCACCACTGCCGAAAGAGAAATCGTCAGAGATATCAAAGAAGAATTA
GCCTACGTCGCCCTCGACTTTGAAGCTGAAATGCAAACTGCTGCCTCATCATCAGCCCTC
GAAAAATCATACGAATTACCAGACGGTCAAGTCATCACCATCGGTAATGAACGTTTCCGT
TGCCCAGAAGCCCTCTTCCAACCATCATTCTTAGGTATGGAATCTGCTGGTATCCACGAA
ACCACCTACAACTCCATCATGAAATGTGATGTTGATATCCGTAAAGATTTATACGGTAAC
GTCGTCTTATCCGGTGGTACAACTATGTTCCCAGGTATTGCTGATCGTATGAACAAAGAA
TTAACTGCTTTAGCCCCATCAACCATGAAAATTAAAATCATTGCTCCACCAGAACGTAAA
TACTCTGTCTGGATTGGTGGATCTATCTTAGCTTCACTCTCAACTTTCCAACAAATGTGG
ATCTCCAAAGAAGAATACGACGAATCAGGTCCATCAATTGTTCACAGAAAATGTTTCTAA
ATTATTTAATAAATAATAAAAAAACAAAT
Length of connected seq. 1279
Full length Seq ID VFB606E
Full length Seq. 
>VFB606E.Seq
AATACTTAATTCAAATATATATATATAAATCATTTAAAAATGGACGGTGAAGATGTTCAA
GCTTTAGTTATCGATAACGGTTCTGGTATGTGTAAAGCCGGTTTTGCTGGTGATGATGCC
CCACGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGACACACTGGTGTTATGGTCGGT
ATGGGTCAAAAAGACTCATATGTAGGTGATGAAGCCCAATCAAAGAGAGGTATCTTAACC
CTCAAATACCCAATTGAACACGGTATCGTTACCAACTGGGATGATATGGAAAAAATCTGG
CATCATACTTTCTACAATGAACTCCGTGTTGCCCCAGAAGAACACCCAGTTCTCTTAACT
GAAGCCCCATTAAATCCAAAGGCCAACAGAGAAAAAATGACCCAAATTATGTTTGAAACC
TTCAACACCCCAGCCATGTACGTCGCCATTCAAGCCGTCTTATCCTTATATGCCTCTGGT
CGTACCACCGGTATTGTTATGGATTCAGGTGATGGTGTCTCCCACACTGTACCAATCTAT
GAAGGTTANGCTTTACCACATGCCATCTTACGTTTAGATTTAGCTGGTCGTGATCTTACC
GATTACATGATGAAAATCTTAACTGAACGTGGTTACTCATTCACCACCACTGCCGAAAGA
GAAATCGTCAGAGATATCAAAGAAGAATTAGCCTACGTCGCCCTCGACTTTGAAGCTGAA
ATGCAAACTGCTGCCTCATCATCAGCCCTCGAAAAATCATACGAATTACCAGACGGTCAA
GTCATCACCATCGGTAATGAACGTTTCCGTTGCCCAGAAGCCCTCTTCCAACCATCATTC
TTAGGTATGGAATCTGCTGGTATCCACGAAACCACCTACAACTCCATCATGAAATGTGAT
GTTGATATCCGTAAAGATTTATACGGTAACGTCGTCTTATCCGGTGGTACAACTATGTTC
CCAGGTATTGCTGATCGTATGAACAAAGAATTAACTGCTTTAGCCCCATCAACCATGAAA
ATTAAAATCATTGCTCCACCAGAACGTAAATACTCTGTCTGGATTGGTGGATCTATCTTA
GCTTCACTCTCAACTTTCCAACAAATGTGGATCTCCAAAGAAGAATACGACGAATCAGGT
CCATCAATTGTTCACAGAAAATGTTTCTAAATTATTTAATAAATAATAAAAAAACAAAT
Length of full length seq. 1199