NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFB595
Library VF
(Link to library)
Clone ID VFB595
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U09552-1
Original site URL
Representative seq. ID VFB595E
(Link to Original site)
Representative DNA sequence 
>VFB595 (VFB595Q) /CSM/VF/VFB5-D/VFB595Q.Seq.d/
GTTGAAGGAAGTGGAGAATGTTTAGTTTGTGAATTTATTTCAGAAAAGATTGTAACTTAT
TTAGAAGCAAATCAAACTGAAACTCAAATTTTACAATATTTAGATAACGATTGTAAACTC
CTTAAATCTGATAACTGGATTTCAACATGTCAAAATTTAATTCAAGAATATGAACCACAA
ATTATTGCAGTTGTTGAAGGATTCATGGCACCAAGTGAACTTTGTCAAAAAATTAAATTC
TGTTCATCATCATCATCAACCAATGATTTCGATTTTATTGGTAGTAGTACAACTGATTGT
GAAATTTGTACATTTATTTCAGGATATGCTGAAAATTTCTTGGAAGAAAATAAAACACTT
GAAGATATAATTAAAGTTGTTGATGATTTCTGTAAAATTCTTCCAGCTGCTTATAAAACT
GATTGTGTAGCTATGGCTTCTAATTATATTCCAGCAATTATTAAAGTGTTGGAAAATGAT
AATTCACCAGGTCAAGTTTGTCAAAAACTTAATCTTTGTCCAGCACCAACTCCAACTTCA
ACACCTTCAACAATCAAGATTGATGTGAATATTAATAAATGTGAAATTTGTGAAACTGTA
ATTGGTTTTGCTGAAAAATATTTAGGTTCAGCATTAGATTCTCAAACTGTAAAGACATTC
CTCCAAAATGAATGTAACAAACTTCCAGGTGTTGTTGGTGATGTTTGTAATGAAGTTGTA
AATGAATATCTTGAATTTGCAGTCACTCAACTCGAAGCTAAATTTAACCCAACTGAAATT
TGTCAATTATTTTCATTCTGTACTGCTGGATCTGAATCATCAGATGCATCTGAAATCAAA
ATTAATAACAACTCATATATTGATTGTCAAATTTGTGAAGTTTTAGCAAATTTCGTAGTC
TATGATAAGAATTATACAACTCATTTCGAAGATAAACTCAATAAATTATGTAGTTGTTTA
TCTCGTAGTTTAATTGAACCATGTCTTAACTTTGTTTCACAATATGGTTCTGATCTTGTA
CAAATCATCGTTCAAGGTGGTGAATCAGCTGCTGAGGCTTGTAATCAAATAGCTCAATGT
CCAACCTCTTCAAGTTCATCATACTCTGGTTCTGCTTCAGGTGCTTCCTCAGGTTCTGCT
TCAGGTTCTGCCTCAGGTT
sequence update 2001. 6. 9
Translated Amino Acid sequence 
VEGSGECLVCEFISEKIVTYLEANQTETQILQYLDNDCKLLKSDNWISTCQNLIQEYEPQ
IIAVVEGFMAPSELCQKIKFCSSSSSTNDFDFIGSSTTDCEICTFISGYAENFLEENKTL
EDIIKVVDDFCKILPAAYKTDCVAMASNYIPAIIKVLENDNSPGQVCQKLNLCPAPTPTS
TPSTIKIDVNINKCEICETVIGFAEKYLGSALDSQTVKTFLQNECNKLPGVVGDVCNEVV
NEYLEFAVTQLEAKFNPTEICQLFSFCTAGSESSDASEIKINNNSYIDCQICEVLANFVV
YDKNYTTHFEDKLNKLCSCLSRSLIEPCLNFVSQYGSDLVQIIVQGGESAAEACNQIAQC
PTSSSSSYSGSASGASSGSASGSASG
Translated Amino Acid sequence (All Frames) 
Frame A:
VEGSGECLVCEFISEKIVTYLEANQTETQILQYLDNDCKLLKSDNWISTCQNLIQEYEPQ
IIAVVEGFMAPSELCQKIKFCSSSSSTNDFDFIGSSTTDCEICTFISGYAENFLEENKTL
EDIIKVVDDFCKILPAAYKTDCVAMASNYIPAIIKVLENDNSPGQVCQKLNLCPAPTPTS
TPSTIKIDVNINKCEICETVIGFAEKYLGSALDSQTVKTFLQNECNKLPGVVGDVCNEVV
NEYLEFAVTQLEAKFNPTEICQLFSFCTAGSESSDASEIKINNNSYIDCQICEVLANFVV
YDKNYTTHFEDKLNKLCSCLSRSLIEPCLNFVSQYGSDLVQIIVQGGESAAEACNQIAQC
PTSSSSSYSGSASGASSGSASGSASG


Frame B:
lkevenv*fvnlfqkrl*li*kqiklklkfyni*itivnslnlitgfqhvki*fknmnhk
llqllkdswhqvnfvkklnsvhhhhqpmisillvvvqlivkfvhlfqdmlkiswkkikhl
ki*lkllmisvkffqllikliv*lwlliifqqllkcwkmiihqvkfvknlifvqhqlqlq
hlqqsrlm*ilinvkfvkl*lvllkni*vqh*ilkl*rhsskmnvtnfqvllvmfvmkl*
mnilnlqslnsklnltqlkfvnyfhsvlldlnhqmhlksklitthilivkfvkf*qis*s
miriiqliskinsinyvvvylvv*lnhvltlfhnmvlilykssfkvvnqllrlvik*lnv
qplqvhhtlvllqvlpqvllqvlpqv


Frame C:
*rkwrmfsl*iyfrkdcnlfrsksn*nsnftifr*rl*tp*i**ldfnmskfnsri*ttn
ycsc*rihgtk*tlskn*ilfiiiinq*frfyw**yn*l*nlyiyfric*kflgrk*nt*
ryn*sc**fl*nssscl*n*lcsygf*lyssny*svgk**ftrsslskt*slsstnsnfn
tfnnqd*cey**m*nl*ncnwfc*kifrfsirfsnckdippk*m*qtsrccw*cl**sck
*is*icshstrs*i*pn*nlsiifilycwi*iirci*nqn**qliy*lsnl*sfskfrsl
**elynsfrr*tq*im*lfis*fn*tms*lcftiwf*sctnhrsrw*isc*gl*snssms
nlfkfiilwfcfrcflrfcfrfclr
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFB595 (VFB595Q) /CSM/VF/VFB5-D/VFB595Q.Seq.d/                       2298   0.0
VFO888 (VFO888Q) /CSM/VF/VFO8-D/VFO888Q.Seq.d/                       1836   0.0
VFJ635 (VFJ635Q) /CSM/VF/VFJ6-B/VFJ635Q.Seq.d/                       1237   0.0
VFB732 (VFB732Q) /CSM/VF/VFB7-B/VFB732Q.Seq.d/                       1187   0.0
VFM205 (VFM205Q) /CSM/VF/VFM2-A/VFM205Q.Seq.d/                       1156   0.0
VFG662 (VFG662Q) /CSM/VF/VFG6-C/VFG662Q.Seq.d/                       1140   0.0
VFD617 (VFD617Q) /CSM/VF/VFD6-A/VFD617Q.Seq.d/                       1138   0.0
SSD712 (SSD712Q) /CSM/SS/SSD7-A/SSD712Q.Seq.d/                       1136   0.0
VFA464 (VFA464Q) /CSM/VF/VFA4-C/VFA464Q.Seq.d/                       1132   0.0
VFH580 (VFH580Q) /CSM/VF/VFH5-D/VFH580Q.Seq.d/                       1088   0.0
own update 2001.12. 4
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

U66367|U66367.1 Dictyostelium discoideum SapA (sapA) mRNA, partial cds.  2282  0.0  1
AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.    38  2e-04  5
U72746|U72746.1 Dictyostelium discoideum cysteine proteinase (cprG) mRNA, complete cds.    48  0.016  2
AF361947|AF361947.1 Dictyostelium discoideum SgcA (sgcA) mRNA, complete cds.    34  0.065  4
AC116977|AC116977.2 Dictyostelium discoideum chromosome 2 map 5515173-5817331 strain AX4, complete sequence.    40  0.12  9
AJ235272|AJ235272.1 Rickettsia prowazekii strain Madrid E, complete genome; segment 3/4.    42  0.30  10
AC117176|AC117176.2 Dictyostelium discoideum chromosome 2 map 5018074-5200947 strain AX4, complete sequence.    34  0.36  11
AF079445|AF079445.1 Dictyostelium discoideum TipC (tipC) gene, complete cds.    38  0.43  2
AC115579|AC115579.2 Dictyostelium discoideum chromosome 2 map 4915084-5005461 strain AX4, complete sequence.    38  0.45  5
BX005352|BX005352.1 Oryza sativa genomic DNA, chromosome 11, BAC clone: 7779.    40  0.70  4
dna update 2003. 9. 5
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U66367_1(U66367|pid:none) Dictyostelium discoideum SapA (sapA) m...   709   0.0
AF196308_1(AF196308|pid:none) Naegleria fowleri naegleriapore A ...   107   8e-22
DQ062781_1(DQ062781|pid:none) Drosophila simulans saposin-relate...   103   9e-21
BC054988_1(BC054988|pid:none) Xenopus laevis hypothetical protei...    97   8e-19
BC080997_1(BC080997|pid:none) Xenopus laevis MGC80725 protein, m...    97   1e-18
AY815893_1(AY815893|pid:none) Schistosoma japonicum SJCHGC01869 ...    95   5e-18
AF108656_1(AF108656|pid:none) Gallus gallus prosaposin mRNA, com...    93   2e-17
(O13035) RecName: Full=Proactivator polypeptide; Contains:   Rec...    93   2e-17
AK223290_1(AK223290|pid:none) Homo sapiens mRNA for prosaposin (...    92   3e-17
(Q6IBQ6) RecName: Full=Proactivator polypeptide; Contains:   Rec...    92   3e-17
protein update 2009. 7.10
PSORT 

 psg: 0.75  gvh: 0.30  alm: 0.41  top: 0.53  tms: 0.00  mit: 0.18  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 32.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system

>> prediction for VFB595 is cyt
5' end seq. ID VFB595F
5' end seq. 
>VFB595F.Seq
GTTGAAGGAAGTGGAGAATGTTTAGTTTGTGAATTTATTTCAGAAAAGATTGTAACTTAT
TTAGAAGCAAATCAAACTGAAACTCAAATTTTACAATATTTAGATAACGATTGTAAACTC
CTTAAATCTGATAACTGGATTTCAACATGTCAAAATTTAATTCAAGAATATGAACCACAA
ATTATTGCAGTTGTTGAAGGATTCATGGCACCAAGTGAACTTTGTCAAAAAATTAAATTC
TGTTCATCATCATCATCAACCAATGATTTCGATTTTATTGGTAGTAGTACAACTGATTGT
GAAATTTGTACATTTATTTCAGGATATGCTGAAAATTTCTTGGAAGAAAATAAAACACTT
GAAGATATAATTAAAGTTGTTGATGATTTCTGTAAAATTCTTCCAGCTGCTTATAAAACT
GATTGTGTAGCTATGGCTTCTAATTATATTCCAGCAATTATTAAAGTGTTGGAAAATGAT
AATTCACCAGGTCAAGTTTGTCAAAAACTTAATCTTTGTCCAGCACCAACTCCAACTTCA
ACACCTTCAACAATCAAGATTGATGTGAATATTAATAAATGTGAAATTTGTGAAACTGTA
AT----------
Length of 5' end seq. 602
3' end seq. ID VFB595Z
3' end seq. 
>VFB595Z.Seq
----------TCCAGCACCAACTCCAACTTCAACACCTTCAACAATCAAGATTGATGTGA
ATATTAATAAATGTGAAATTTGTGAAACTGTAATTGGTTTTGCTGAAAAATATTTAGGTT
CAGCATTAGATTCTCAAACTGTAAAGACATTCCTCCAAAATGAATGTAACAAACTTCCAG
GTGTTGTTGGTGATGTTTGTAATGAAGTTGTAAATGAATATCTTGAATTTGCAGTCACTC
AACTCGAAGCTAAATTTAACCCAACTGAAATTTGTCAATTATTTTCATTCTGTACTGCTG
GATCTGAATCATCAGATGCATCTGAAATCAAAATTAATAACAACTCATATATTGATTGTC
AAATTTGTGAAGTTTTAGCAAATTTCGTAGTCTATGATAAGAATTATACAACTCATTTCG
AAGATAAACTCAATAAATTATGTAGTTGTTTATCTCGTAGTTTAATTGAACCATGTCTTA
ACTTTGTTTCACAATATGGTTCTGATCTTGTACAAATCATCGTTCAAGGTGGTGAATCAG
CTGCTGAGGCTTGTAATCAAATAGCTCAATGTCCAACCTCTTCAAGTTCATCATACTCTG
GTTCTGCTTCAGGTGCTTCCTCAGGTTCTGCTTCAGGTTCTGCCTCAGGTT
Length of 3' end seq. 641
Connected seq. ID VFB595P
Connected seq. 
>VFB595P.Seq
GTTGAAGGAAGTGGAGAATGTTTAGTTTGTGAATTTATTTCAGAAAAGATTGTAACTTAT
TTAGAAGCAAATCAAACTGAAACTCAAATTTTACAATATTTAGATAACGATTGTAAACTC
CTTAAATCTGATAACTGGATTTCAACATGTCAAAATTTAATTCAAGAATATGAACCACAA
ATTATTGCAGTTGTTGAAGGATTCATGGCACCAAGTGAACTTTGTCAAAAAATTAAATTC
TGTTCATCATCATCATCAACCAATGATTTCGATTTTATTGGTAGTAGTACAACTGATTGT
GAAATTTGTACATTTATTTCAGGATATGCTGAAAATTTCTTGGAAGAAAATAAAACACTT
GAAGATATAATTAAAGTTGTTGATGATTTCTGTAAAATTCTTCCAGCTGCTTATAAAACT
GATTGTGTAGCTATGGCTTCTAATTATATTCCAGCAATTATTAAAGTGTTGGAAAATGAT
AATTCACCAGGTCAAGTTTGTCAAAAACTTAATCTTTGTCCAGCACCAACTCCAACTTCA
ACACCTTCAACAATCAAGATTGATGTGAATATTAATAAATGTGAAATTTGTGAAACTGTA
AT----------TCCAGCACCAACTCCAACTTCAACACCTTCAACAATCAAGATTGATGT
GAATATTAATAAATGTGAAATTTGTGAAACTGTAATTGGTTTTGCTGAAAAATATTTAGG
TTCAGCATTAGATTCTCAAACTGTAAAGACATTCCTCCAAAATGAATGTAACAAACTTCC
AGGTGTTGTTGGTGATGTTTGTAATGAAGTTGTAAATGAATATCTTGAATTTGCAGTCAC
TCAACTCGAAGCTAAATTTAACCCAACTGAAATTTGTCAATTATTTTCATTCTGTACTGC
TGGATCTGAATCATCAGATGCATCTGAAATCAAAATTAATAACAACTCATATATTGATTG
TCAAATTTGTGAAGTTTTAGCAAATTTCGTAGTCTATGATAAGAATTATACAACTCATTT
CGAAGATAAACTCAATAAATTATGTAGTTGTTTATCTCGTAGTTTAATTGAACCATGTCT
TAACTTTGTTTCACAATATGGTTCTGATCTTGTACAAATCATCGTTCAAGGTGGTGAATC
AGCTGCTGAGGCTTGTAATCAAATAGCTCAATGTCCAACCTCTTCAAGTTCATCATACTC
TGGTTCTGCTTCAGGTGCTTCCTCAGGTTCTGCTTCAGGTTCTGCCTCAGGTT
Length of connected seq. 1243
Full length Seq ID VFB595E
Full length Seq. 
>VFB595E.Seq
GTTGAAGGAAGTGGAGAATGTTTAGTTTGTGAATTTATTTCAGAAAAGATTGTAACTTAT
TTAGAAGCAAATCAAACTGAAACTCAAATTTTACAATATTTAGATAACGATTGTAAACTC
CTTAAATCTGATAACTGGATTTCAACATGTCAAAATTTAATTCAAGAATATGAACCACAA
ATTATTGCAGTTGTTGAAGGATTCATGGCACCAAGTGAACTTTGTCAAAAAATTAAATTC
TGTTCATCATCATCATCAACCAATGATTTCGATTTTATTGGTAGTAGTACAACTGATTGT
GAAATTTGTACATTTATTTCAGGATATGCTGAAAATTTCTTGGAAGAAAATAAAACACTT
GAAGATATAATTAAAGTTGTTGATGATTTCTGTAAAATTCTTCCAGCTGCTTATAAAACT
GATTGTGTAGCTATGGCTTCTAATTATATTCCAGCAATTATTAAAGTGTTGGAAAATGAT
AATTCACCAGGTCAAGTTTGTCAAAAACTTAATCTTTGTCCAGCACCAACTCCAACTTCA
ACACCTTCAACAATCAAGATTGATGTGAATATTAATAAATGTGAAATTTGTGAAACTGTA
ATTGGTTTTGCTGAAAAATATTTAGGTTCAGCATTAGATTCTCAAACTGTAAAGACATTC
CTCCAAAATGAATGTAACAAACTTCCAGGTGTTGTTGGTGATGTTTGTAATGAAGTTGTA
AATGAATATCTTGAATTTGCAGTCACTCAACTCGAAGCTAAATTTAACCCAACTGAAATT
TGTCAATTATTTTCATTCTGTACTGCTGGATCTGAATCATCAGATGCATCTGAAATCAAA
ATTAATAACAACTCATATATTGATTGTCAAATTTGTGAAGTTTTAGCAAATTTCGTAGTC
TATGATAAGAATTATACAACTCATTTCGAAGATAAACTCAATAAATTATGTAGTTGTTTA
TCTCGTAGTTTAATTGAACCATGTCTTAACTTTGTTTCACAATATGGTTCTGATCTTGTA
CAAATCATCGTTCAAGGTGGTGAATCAGCTGCTGAGGCTTGTAATCAAATAGCTCAATGT
CCAACCTCTTCAAGTTCATCATACTCTGGTTCTGCTTCAGGTGCTTCCTCAGGTTCTGCT
TCAGGTTCTGCCTCAGGTT
Length of full length seq. 1159