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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFB594
Library VF
(Link to library)
Clone ID VFB594
Atlas ID -
NBRP ID
dictyBase ID
Link to Contig Contig-U09553-1
Original site URL
Representative seq. ID VFB594P
(Link to Original site)
Representative DNA sequence 
>VFB594 (VFB594Q) /CSM/VF/VFB5-D/VFB594Q.Seq.d/
ACTGTTGGCTCTACTGGNAAATAAAAATATATATATAATATATATAAAATGGAATCTGAA
AATAAAAATAATAATAAAAAAGTTAAAGAAGAATCTAAAGTTAAAGTTAAATTTATAACT
AATGATGCAAATATTAGAGTTACAGATACACCAATTGCTGTACCAGTTCGTTTAGGTCGT
CTTGGTTTATCAGAAGTAATTCATCATTTAAGAGAAGAAGAAAACGAAACATCAAAACCA
TTCGATTTCCTTATTAATXXXXXXXXXXGTTTCACCAGATTCATCACGTATTGTATCGGC
TTCAATGGATTCAACAATTAAATTATGGAGTATTAAAGACATACCAAATCAACATGCAAC
CACTTCAAGCTCCATTGAAAAGAGTAATAGTAAAAAACGTAGATTAGCAAACAACACCAA
TAATGAAACAGCAGCCGTCGACCAACAATCAGAGATCATTCATAATATAACGGAGTCATT
GGCATCGGTAACCGTACCAAACAGTCAAGGTGTTACAGTGGTCAATTGGACAACCCAATT
CCAAATGTTGTCAGGTTCAATGGATTCAAAAATTAGATTATGGGATGTTAGTACATTGGT
TGCAAATGATACAATCCCTACACCTGCTCCATTAACCGATTTGGATTATTCAATGGAATC
TGGTTTAATTGTAACTGCACATAAAGATAGAATCGTTAGAATTTGGGATCCAAGATCATC
CGATGAAACTAAATCTCAAACTCAATCTTTAATCTCTCATAAAACTTGGGTAACAAGTGT
AAATTGGAATCCATCTTCAAAATATCATTGTTGTTCTACCTCTCATGATGGTACCGTCAA
ATATTGGGATATCCGTACAAAGATTCCATTATACACTATTGATACTTTAGAGAAATCAAA
AGATAAAGTTTTATCATCATCTTTCATTTCAAATAAAAATAAAAATATTAATGATTATTC
AATTGTTTCTGGTGGTACTGATTCAAAATTAAGAATTCATTATAATATAAACAAGAACAA
CAACAATAAATAAATAAATAAATAAAAATAATTAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
tvgstgk*KYIYNIYKMESENKNNNKKVKEESKVKVKFITNDANIRVTDTPIAVPVRLGR
LGLSEVIHHLREEENETSKPFDFLIN---

---VSPDSSRIVSASMDSTIKLWSIKDIPNQHATTSSSIEKSNSKKRRLANNTNNETAAV
DQQSEIIHNITESLASVTVPNSQGVTVVNWTTQFQMLSGSMDSKIRLWDVSTLVANDTIP
TPAPLTDLDYSMESGLIVTAHKDRIVRIWDPRSSDETKSQTQSLISHKTWVTSVNWNPSS
KYHCCSTSHDGTVKYWDIRTKIPLYTIDTLEKSKDKVLSSSFISNKNKNINDYSIVSGGT
DSKLRIHYNINKNNNNK*ink*k*l
Translated Amino Acid sequence (All Frames) 
Frame A:
tvgstgk*KYIYNIYKMESENKNNNKKVKEESKVKVKFITNDANIRVTDTPIAVPVRLGR
LGLSEVIHHLREEENETSKPFDFLIN---

---VSPDSSRIVSASMDSTIKLWSIKDIPNQHATTSSSIEKSNSKKRRLANNTNNETAAV
DQQSEIIHNITESLASVTVPNSQGVTVVNWTTQFQMLSGSMDSKIRLWDVSTLVANDTIP
TPAPLTDLDYSMESGLIVTAHKDRIVRIWDPRSSDETKSQTQSLISHKTWVTSVNWNPSS
KYHCCSTSHDGTVKYWDIRTKIPLYTIDTLEKSKDKVLSSSFISNKNKNINDYSIVSGGT
DSKLRIHYNINKNNNNK*ink*k*l

Frame B:
llallxnkniyiiyikwnlkikiiikklkknlklklnl*lmmqilelqihqllyqfv*vv
lvyqk*fii*ekkktkhqnhsisll---

---fhqihhvlyrlqwiqqlnygvlktyqinmqplqaplkrvivknvd*qttpimkqqps
tnnqrsfii*rshwhr*pyqtvkvlqwsigqpnskccqvqwiqkldygmlvhwlqmiqsl
hllh*piwiiqwnlv*l*lhikieslefgiqdhpmklnlklnl*sliklg*qv*igihlq
niivvlplmmvpsnigisvqrfhytllil*rnqkikfyhhlsfqikikilmiiqlflvvl
iqn*efiii*trtttink*inknn*

Frame C:
cwlywxikiyi*yi*ngi*k*k***ks*rri*s*s*iyn**cky*syrytncctssfrss
wfirsnssfkrrrkrniktirfpy*---

---ftrfitycigfngfnn*imey*rhtkstcnhfklh*ke***kt*iskqhq**nssrr
ptirdhs*yngvigignrtkqsrcysgqldnpipnvvrfngfkn*imgc*yigck*ynpy
tcsinrfglfngiwfncnct*r*nr*nlgskiir*n*isnsifnls*nlgnkcklesifk
isllfyls*wyrqilgypykdsiihy*yfreikr*sfiiifhfk*k*ky**lfncfwwy*
fkiknsl*ykqeqqq*ink*ikii
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFB594 (VFB594Q) /CSM/VF/VFB5-D/VFB594Q.Seq.d/                       1701   0.0
VFM608 (VFM608Q) /CSM/VF/VFM6-A/VFM608Q.Seq.d/                       1388   0.0
AHI766 (AHI766Q) /CSM/AH/AHI7-C/AHI766Q.Seq.d/                         44   0.004
SHL421 (SHL421Q) /CSM/SH/SHL4-A/SHL421Q.Seq.d/                         42   0.017
SHK140 (SHK140Q) /CSM/SH/SHK1-B/SHK140Q.Seq.d/                         42   0.017
SHC684 (SHC684Q) /CSM/SH/SHC6-D/SHC684Q.Seq.d/                         42   0.017
SHC520 (SHC520Q) /CSM/SH/SHC5-A/SHC520Q.Seq.d/                         42   0.017
SHC483 (SHC483Q) /CSM/SH/SHC4-D/SHC483Q.Seq.d/                         42   0.017
SHA756 (SHA756Q) /CSM/SH/SHA7-C/SHA756Q.Seq.d/                         42   0.017
CHK432 (CHK432Q) /CSM/CH/CHK4-B/CHK432Q.Seq.d/                         42   0.017
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AF379886|AF379886.1 Brachyserphus abruptus 16S ribosomal RNA gene, partial sequence; mitochondrial gene for mitochondrial product.    52  0.015  1
AX349002|AX349002.1 Sequence 460 from Patent WO0202807.           50  0.058  1
AY005798|AY005798.1 Trypanosoma cruzi MSH2 mRNA, complete cds.    50  0.058  1
AP003137|AP003137.2 Staphylococcus aureus subsp. aureus N315 genomic DNA, complete genome, section 9/10.    38  0.10  8
AC000103|AC000103.2 Genomic sequence for Arabidopsis thaliana BAC F21J9 from chromosome I, complete sequence.    44  0.14  3
AC115612|AC115612.2 Dictyostelium discoideum chromosome 2 map 6245135-6357017 strain AX4, complete sequence.    40  0.19  5
AL035475|AL035475.7 Plasmodium falciparum MAL4P2.                 46  0.20  5
AC091181|AC091181.8 Homo sapiens chromosome 17, clone RP11-426D19, complete sequence.    34  0.20  7
BZ046915|BZ046915.1 lki19h11.b1 B.oleracea002 Brassica oleracea genomic, DNA sequence.    48  0.23  1
BX005223|BX005223.7 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone.    48  0.23  1
dna update 2003. 9. 5
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BT034751_1(BT034751|pid:none) Zea mays full-length cDNA clone ZM...   120   6e-26
AL391143_16(AL391143|pid:none) Arabidopsis thaliana DNA chromoso...   107   5e-22
(Q5BJ90) RecName: Full=Ribosome biogenesis protein wdr12; AltNam...    98   4e-19
(Q6NX08) RecName: Full=Ribosome biogenesis protein wdr12; AltNam...    98   6e-19
BC044198_1(BC044198|pid:none) Danio rerio WD repeat domain 12, m...    96   2e-18
AK001743_1(AK001743|pid:none) Homo sapiens cDNA FLJ10881 fis, cl...    94   8e-18
(Q9GZL7) RecName: Full=Ribosome biogenesis protein WDR12; AltNam...    94   8e-18
(Q5REE6) RecName: Full=Ribosome biogenesis protein WDR12; AltNam...    94   8e-18
(A3LQ86) RecName: Full=Ribosome biogenesis protein YTM1;  &CP000...    92   2e-17
(A8QB65) RecName: Full=Ribosome biogenesis protein WDR12 homolog;      92   2e-17
protein update 2009. 6.17
PSORT 

 psg: 0.29  gvh: 0.39  alm: 0.44  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: nuclear
	 32.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: vesicles of secretory system

>> prediction for VFB594 is nuc
5' end seq. ID VFB594F
5' end seq. 
>VFB594F.Seq
ACTGTTGGCTCTACTGGNAAATAAAAATATATATATAATATATATAAAATGGAATCTGAA
AATAAAAATAATAATAAAAAAGTTAAAGAAGAATCTAAAGTTAAAGTTAAATTTATAACT
AATGATGCAAATATTAGAGTTACAGATACACCAATTGCTGTACCAGTTCGTTTAGGTCGT
CTTGGTTTATCAGAAGTAATTCATCATTTAAGAGAAGAAGAAAACGAAACATCAAAACCA
TTCGATTTCCTTATTAAT----------
Length of 5' end seq. 258
3' end seq. ID VFB594Z
3' end seq. 
>VFB594Z.Seq
----------GTTTCACCAGATTCATCACGTATTGTATCGGCTTCAATGGATTCAACAAT
TAAATTATGGAGTATTAAAGACATACCAAATCAACATGCAACCACTTCAAGCTCCATTGA
AAAGAGTAATAGTAAAAAACGTAGATTAGCAAACAACACCAATAATGAAACAGCAGCCGT
CGACCAACAATCAGAGATCATTCATAATATAACGGAGTCATTGGCATCGGTAACCGTACC
AAACAGTCAAGGTGTTACAGTGGTCAATTGGACAACCCAATTCCAAATGTTGTCAGGTTC
AATGGATTCAAAAATTAGATTATGGGATGTTAGTACATTGGTTGCAAATGATACAATCCC
TACACCTGCTCCATTAACCGATTTGGATTATTCAATGGAATCTGGTTTAATTGTAACTGC
ACATAAAGATAGAATCGTTAGAATTTGGGATCCAAGATCATCCGATGAAACTAAATCTCA
AACTCAATCTTTAATCTCTCATAAAACTTGGGTAACAAGTGTAAATTGGAATCCATCTTC
AAAATATCATTGTTGTTCTACCTCTCATGATGGTACCGTCAAATATTGGGATATCCGTAC
AAAGATTCCATTATACACTATTGATACTTTAGAGAAATCAAAAGATAAAGTTTTATCATC
ATCTTTCATTTCAAATAAAAATAAAAATATTAATGATTATTCAATTGTTTCTGGTGGTAC
TGATTCAAAATTAAGAATTCATTATAATATAAACAAGAACAACAACAATAAATAAATAAA
TAAATAAAAATAATTAA
Length of 3' end seq. 787
Connected seq. ID VFB594P
Connected seq. 
>VFB594P.Seq
ACTGTTGGCTCTACTGGNAAATAAAAATATATATATAATATATATAAAATGGAATCTGAA
AATAAAAATAATAATAAAAAAGTTAAAGAAGAATCTAAAGTTAAAGTTAAATTTATAACT
AATGATGCAAATATTAGAGTTACAGATACACCAATTGCTGTACCAGTTCGTTTAGGTCGT
CTTGGTTTATCAGAAGTAATTCATCATTTAAGAGAAGAAGAAAACGAAACATCAAAACCA
TTCGATTTCCTTATTAAT----------GTTTCACCAGATTCATCACGTATTGTATCGGC
TTCAATGGATTCAACAATTAAATTATGGAGTATTAAAGACATACCAAATCAACATGCAAC
CACTTCAAGCTCCATTGAAAAGAGTAATAGTAAAAAACGTAGATTAGCAAACAACACCAA
TAATGAAACAGCAGCCGTCGACCAACAATCAGAGATCATTCATAATATAACGGAGTCATT
GGCATCGGTAACCGTACCAAACAGTCAAGGTGTTACAGTGGTCAATTGGACAACCCAATT
CCAAATGTTGTCAGGTTCAATGGATTCAAAAATTAGATTATGGGATGTTAGTACATTGGT
TGCAAATGATACAATCCCTACACCTGCTCCATTAACCGATTTGGATTATTCAATGGAATC
TGGTTTAATTGTAACTGCACATAAAGATAGAATCGTTAGAATTTGGGATCCAAGATCATC
CGATGAAACTAAATCTCAAACTCAATCTTTAATCTCTCATAAAACTTGGGTAACAAGTGT
AAATTGGAATCCATCTTCAAAATATCATTGTTGTTCTACCTCTCATGATGGTACCGTCAA
ATATTGGGATATCCGTACAAAGATTCCATTATACACTATTGATACTTTAGAGAAATCAAA
AGATAAAGTTTTATCATCATCTTTCATTTCAAATAAAAATAAAAATATTAATGATTATTC
AATTGTTTCTGGTGGTACTGATTCAAAATTAAGAATTCATTATAATATAAACAAGAACAA
CAACAATAAATAAATAAATAAATAAAAATAATTAA
Length of connected seq. 1045
Full length Seq ID -
Full length Seq. -
Length of full length seq. -