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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFB574
Library VF
(Link to library)
Clone ID VFB574
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16382-1
Original site URL
Representative seq. ID VFB574P
(Link to Original site)
Representative DNA sequence 
>VFB574 (VFB574Q) /CSM/VF/VFB5-D/VFB574Q.Seq.d/
AGTTTTAATAAACAAATATTTAAATATAATACAACAATAAAATGGACGGTGAAGATGTTC
AAGCTTTAGTTATCGATAATGGTTCTGGTATGTGTAAAGCCGGTTTTGCTGGTGATGATG
CCCCACGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGACACACTGGTGTTATGGTCG
GTATGGGTCAAAAAGACTCTTACGTAGGTGATGAAGCCCAATCAAAGAGAGGTATCTTAA
CCCTCAAATACCCAATTGAACACGGTATTGTTACCAACTGGGATGATATGGAAAAAATCT
GGCATCATACTTTCTACAATGAACTCCGTGTTGCTCCAGAAGAACACCCAGTTCTCTTAA
CTGAAGCCCCATTAAATCCAAAGGCCAACAGAGAAAAAATGACCCAAATTATGTTTGAAA
CCTTCAACACCCCAGCCATGTACGTTGCCATTCAAGCTGTCTTATCCTTATATGCCTCTG
GTCGTACCACCGGTATTGNTATGGATTCXXXXXXXXXXTGAAGGTTATGCCTTACCACAT
GCCATCTTACGTTTAGATTTAGCTGGTCGTGATCTTACTGATTACATGATGAAAATCTTA
ACTGAACGTGGTTACTCATTCACCACCACTGCTGAAAGAGAAATCGTCAGAGATATCAAA
GAAGAATTAGCCTACGTCGCCCTCGACTTTGAAGCTGAAATGCAAACTGCTGCCTCATCA
TCAGCCCTCGAAAAATCATACGAATTACCAGACGGTCAAGTCATCACCATCGGTAATGAA
CGTTTCCGTTGCCCAGAAGCCCTCTTCCAACCATCATTCTTAGGTATGGAATCAGCTGGT
ATCCACGAAACCACCTATAACTCCATCATGAAATGTGATGTTGATATCCGTAAAGATTTA
TACGGTAATGTCGTCTTATCTGGTGGTACAACTATGTTCCCAGGTATTGCTGATCGTATG
AACAAAGAATTAACTGCTTTAGCCCCATCAACCATGAAAATTAAAATCATTGCTCCACCA
GAACGTAAATACTCTGTCTGGATTGGTGGATCTATCTTAGCTTCACTCTCAACTTTCCAA
CAAATGTGGATCTCCAAAGAAGAATATGACGAATCAGGTCCATCAATTGTCCACAGAAAA
TGTTTCTAAACAATTAAAACTAGTGATGAAAGTGCTTCTCACACAAACAAATATATTTAA
ATGT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
f**tni*i*YNNKMDGEDVQALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHTGVMVG
MGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLT
EAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIXMD---

---EGYALPHAILRLDLAGRDLTDYMMKILTERGYSFTTTAEREIVRDIKEELAYVALDF
EAEMQTAASSSALEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESAGIHETTYNSIM
KCDVDIRKDLYGNVVLSGGTTMFPGIADRMNKELTALAPSTMKIKIIAPPERKYSVWIGG
SILASLSTFQQMWISKEEYDESGPSIVHRKCF*tiktsdesashtnkyi*m
Translated Amino Acid sequence (All Frames) 
Frame A:
sfnkqifkynttikwtvkmfkl*lsimvlvcvkpvllvmmphvlfshqllvvqdtlvlws
vwvkktlt*vmkpnqrevs*psntqlntvllptgmiwkksgiilstmnsvllqkntqfs*
lkph*iqrptekk*pklclkpstpqpctlpfklsypymplvvppvlxwi---

---*rlclttchltfrfsws*sy*lhdenln*twllihhhc*krnrqryqrrislrrprl
*s*nanccliisprkiiritrrsshhhr**tfplprsplptiilrygiswyprnhl*lhh
em*c*yp*rfir*crliwwynyvpryc*syeqrincfspinhen*nhcstrt*ilcldww
iylsftlnfptnvdlqrri*rirsincpqkmflnn*n***kcfshkqiyln

Frame B:
vlinkylniiqq*ngr*rcssfsyr*wfwyv*srfcw**cptccfpincwsskthwcygr
ygskrllrr**spikerylnpqipn*trycyqlg*ygknlasyflq*tpccsrrtpssln
*spikskgqqrkndpnyv*nlqhpshvrchsscliliclwsyhryxygf---

---EGYALPHAILRLDLAGRDLTDYMMKILTERGYSFTTTAEREIVRDIKEELAYVALDF
EAEMQTAASSSALEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESAGIHETTYNSIM
KCDVDIRKDLYGNVVLSGGTTMFPGIADRMNKELTALAPSTMKIKIIAPPERKYSVWIGG
SILASLSTFQQMWISKEEYDESGPSIVHRKCF*tiktsdesashtnkyi*m

Frame C:
f**tni*i*YNNKMDGEDVQALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHTGVMVG
MGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLT
EAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIXMD---

---kvmpyhmpsyv*i*lvvillit**ks*lnvvthsppllkeksseiskkn*ptspstl
klkckllphhqpsknhtnyqtvksspsvmnvsvaqkpssnhhs*vwnqlvstkppitps*
nvmlisvkiytvmssylvvqlcsqvlliv*tkn*ll*phqp*klksllhqnvntlsglvd
ls*lhsqlsnkcgspkknmtnqvhqlstenvskqlklvmkvlltqtnifkc
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFB574 (VFB574Q) /CSM/VF/VFB5-D/VFB574Q.Seq.d/                       2321   0.0
VFI269 (VFI269Q) /CSM/VF/VFI2-C/VFI269Q.Seq.d/                       1352   0.0
VFE460 (VFE460Q) /CSM/VF/VFE4-C/VFE460Q.Seq.d/                       1352   0.0
VFE112 (VFE112Q) /CSM/VF/VFE1-A/VFE112Q.Seq.d/                       1352   0.0
SFG507 (SFG507Q) /CSM/SF/SFG5-A/SFG507Q.Seq.d/                       1352   0.0
AFK465 (AFK465Q) /CSM/AF/AFK4-C/AFK465Q.Seq.d/                       1352   0.0
VFO858 (VFO858Q) /CSM/VF/VFO8-C/VFO858Q.Seq.d/                       1350   0.0
VFM807 (VFM807Q) /CSM/VF/VFM8-A/VFM807Q.Seq.d/                       1344   0.0
VFD891 (VFD891Q) /CSM/VF/VFD8-D/VFD891Q.Seq.d/                       1344   0.0
VFA623 (VFA623Q) /CSM/VF/VFA6-A/VFA623Q.Seq.d/                       1344   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.  1352  0.0  4
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.  1178  0.0  5
X03281|X03281.1 Dictyostelium discoideum gene for actin A8.     1172  0.0  2
M14146|M14146.1 D.discoideum actin 15 gene, complete cds.       1110  0.0  3
AC115579|AC115579.2 Dictyostelium discoideum chromosome 2 map 4915084-5005461 strain AX4, complete sequence.  1110  0.0  4
AC115577|AC115577.2 Dictyostelium discoideum chromosome 2 map 4657875-4914984 strain AX4, complete sequence.   967  0.0  13
X03282|X03282.1 dictyostelium discoideum gene for actin A12.     926  0.0  2
AC116956|AC116956.2 Dictyostelium discoideum chromosome 2 map 1418423-1684967 strain AX4, complete sequence.   920  0.0  7
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.   815  0.0  5
U25660|U25660.1 Dictyostelium discoideum actin gene, partial cds.   791  0.0  2
dna update 2003. 9. 3
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54H30) RecName: Full=Major actin; AltName: Full=Actin-1; AltNa...   416   0.0
(Q553U6) RecName: Full=Putative actin-22;  &AC116305_3(AC116305|...   412   0.0
B23412(B23412)actin 12 - slime mold (Dictyostelium discoideum)        416   0.0
ATDO(A93223;A92871;S67999;A03004)actin - slime mold (Dictyosteli...   412   0.0
(P02576) RecName: Full=Actin, plasmodial isoform;  &ATFY(A29044;...   411   0.0
X03282_1(X03282|pid:none) dictyostelium discoideum gene for acti...   416   0.0
(P07829) RecName: Full=Actin-3; AltName: Full=Actin-3-sub 1;          408   0.0
AY294154_1(AY294154|pid:none) Thecamoeba similis actin mRNA, com...   405   0.0
(P02578) RecName: Full=Actin-1;  &ATAX(A92886;A03004) &V00002_1(...   404   0.0
AF082863_1(AF082863|pid:none) Dreissena polymorpha cytoplasmic a...   399   0.0
protein update 2009. 6.17
PSORT 

 psg: 0.75  gvh: 0.34  alm: 0.42  top: 0.53  tms: 0.00  mit: 0.15  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 36.0 %: nuclear
	 20.0 %: cytoskeletal
	  4.0 %: peroxisomal

>> prediction for VFB574 is cyt
5' end seq. ID VFB574F
5' end seq. 
>VFB574F.Seq
AGTTTTAATAAACAAATATTTAAATATAATACAACAATAAAATGGACGGTGAAGATGTTC
AAGCTTTAGTTATCGATAATGGTTCTGGTATGTGTAAAGCCGGTTTTGCTGGTGATGATG
CCCCACGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGACACACTGGTGTTATGGTCG
GTATGGGTCAAAAAGACTCTTACGTAGGTGATGAAGCCCAATCAAAGAGAGGTATCTTAA
CCCTCAAATACCCAATTGAACACGGTATTGTTACCAACTGGGATGATATGGAAAAAATCT
GGCATCATACTTTCTACAATGAACTCCGTGTTGCTCCAGAAGAACACCCAGTTCTCTTAA
CTGAAGCCCCATTAAATCCAAAGGCCAACAGAGAAAAAATGACCCAAATTATGTTTGAAA
CCTTCAACACCCCAGCCATGTACGTTGCCATTCAAGCTGTCTTATCCTTATATGCCTCTG
GTCGTACCACCGGTATTGNTATGGATTC----------
Length of 5' end seq. 508
3' end seq. ID VFB574Z
3' end seq. 
>VFB574Z.Seq
----------TGAAGGTTATGCCTTACCACATGCCATCTTACGTTTAGATTTAGCTGGTC
GTGATCTTACTGATTACATGATGAAAATCTTAACTGAACGTGGTTACTCATTCACCACCA
CTGCTGAAAGAGAAATCGTCAGAGATATCAAAGAAGAATTAGCCTACGTCGCCCTCGACT
TTGAAGCTGAAATGCAAACTGCTGCCTCATCATCAGCCCTCGAAAAATCATACGAATTAC
CAGACGGTCAAGTCATCACCATCGGTAATGAACGTTTCCGTTGCCCAGAAGCCCTCTTCC
AACCATCATTCTTAGGTATGGAATCAGCTGGTATCCACGAAACCACCTATAACTCCATCA
TGAAATGTGATGTTGATATCCGTAAAGATTTATACGGTAATGTCGTCTTATCTGGTGGTA
CAACTATGTTCCCAGGTATTGCTGATCGTATGAACAAAGAATTAACTGCTTTAGCCCCAT
CAACCATGAAAATTAAAATCATTGCTCCACCAGAACGTAAATACTCTGTCTGGATTGGTG
GATCTATCTTAGCTTCACTCTCAACTTTCCAACAAATGTGGATCTCCAAAGAAGAATATG
ACGAATCAGGTCCATCAATTGTCCACAGAAAATGTTTCTAAACAATTAAAACTAGTGATG
AAAGTGCTTCTCACACAAACAAATATATTTAAATGT
Length of 3' end seq. 686
Connected seq. ID VFB574P
Connected seq. 
>VFB574P.Seq
AGTTTTAATAAACAAATATTTAAATATAATACAACAATAAAATGGACGGTGAAGATGTTC
AAGCTTTAGTTATCGATAATGGTTCTGGTATGTGTAAAGCCGGTTTTGCTGGTGATGATG
CCCCACGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGACACACTGGTGTTATGGTCG
GTATGGGTCAAAAAGACTCTTACGTAGGTGATGAAGCCCAATCAAAGAGAGGTATCTTAA
CCCTCAAATACCCAATTGAACACGGTATTGTTACCAACTGGGATGATATGGAAAAAATCT
GGCATCATACTTTCTACAATGAACTCCGTGTTGCTCCAGAAGAACACCCAGTTCTCTTAA
CTGAAGCCCCATTAAATCCAAAGGCCAACAGAGAAAAAATGACCCAAATTATGTTTGAAA
CCTTCAACACCCCAGCCATGTACGTTGCCATTCAAGCTGTCTTATCCTTATATGCCTCTG
GTCGTACCACCGGTATTGNTATGGATTC----------TGAAGGTTATGCCTTACCACAT
GCCATCTTACGTTTAGATTTAGCTGGTCGTGATCTTACTGATTACATGATGAAAATCTTA
ACTGAACGTGGTTACTCATTCACCACCACTGCTGAAAGAGAAATCGTCAGAGATATCAAA
GAAGAATTAGCCTACGTCGCCCTCGACTTTGAAGCTGAAATGCAAACTGCTGCCTCATCA
TCAGCCCTCGAAAAATCATACGAATTACCAGACGGTCAAGTCATCACCATCGGTAATGAA
CGTTTCCGTTGCCCAGAAGCCCTCTTCCAACCATCATTCTTAGGTATGGAATCAGCTGGT
ATCCACGAAACCACCTATAACTCCATCATGAAATGTGATGTTGATATCCGTAAAGATTTA
TACGGTAATGTCGTCTTATCTGGTGGTACAACTATGTTCCCAGGTATTGCTGATCGTATG
AACAAAGAATTAACTGCTTTAGCCCCATCAACCATGAAAATTAAAATCATTGCTCCACCA
GAACGTAAATACTCTGTCTGGATTGGTGGATCTATCTTAGCTTCACTCTCAACTTTCCAA
CAAATGTGGATCTCCAAAGAAGAATATGACGAATCAGGTCCATCAATTGTCCACAGAAAA
TGTTTCTAAACAATTAAAACTAGTGATGAAAGTGCTTCTCACACAAACAAATATATTTAA
ATGT
Length of connected seq. 1194
Full length Seq ID -
Full length Seq. -
Length of full length seq. -