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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFB569
Library VF
(Link to library)
Clone ID VFB569
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15456-1
Original site URL
Representative seq. ID VFB569E
(Link to Original site)
Representative DNA sequence 
>VFB569 (VFB569Q) /CSM/VF/VFB5-C/VFB569Q.Seq.d/
GTCACCTGACTTACATAAAAGAAAATGAAATTACTTATTTTAACTTTATTTTTAGCTACT
ATTGTTTTAGCTCAAGCTTTAACAGTACCATTAAACTTCCATCAAGCTTCAAGAGAATCT
AGAAGAAGAGTTCCACAAAAATGGTCAAACAGATTATCTGCTCTCAATGCTGGTACCACA
ATCCCAATTTCAGATTTTGAAGATGCTCAATACTATGGTGCCATTACCATTGGTACCCCA
GGTCAAGCCTTCAAAGTAGTTTTCGATACTGGTTCATCCAACTTGTGGATTCCATCAAAG
AAATGTCCAATCACTGTTGTTGCATGTGATTTACATAACAAATATAACAGCGGTGCCTCA
AGCACATATGTCGCCAACGGAACTGATTTCACCATCCAATACGGTAGTGGTGCTATGTCA
GGTTTTGTCTCTCAAGATTCCGTCACTGTNGGTTCATTAACTGTTAAAGATCAATTATTC
GCTGAAGCCACTGCCGAACCAGGTATTGCTTTCGATTTCGCCAAATTCGATGGTATTTTA
GGTCTTGCTTTCCAATCCATCTCTGTTAATTCAATTCCACCAGTCTTTTACAACATGTTA
TCACAAGGTTTAGTTTCATCAACACTCTTCTCCTTCTGGTTATCAAGAACTCCAGGTGCC
AACGGTGGTGAACTCTCATTCGGTTCAATCGATAACACCAAATACACTGGTGACATTACC
TACGTCCCATTAACCAACGAAACCTATTGGGAATTCGTTATGGATGACTTTGCTATCGAT
GGTCAATCAGCTGGTTTCTGTGGTACTACTTGTCACGCAATTTGCGATTCAGGTACATCA
CTCATTGCTGGTCCAATGGCTGATATTACTGCCCTCAATGAAAAATTAGGTGCTGTCATC
TTAAATGGTGAAGGTGTCTTCTCTGATTGTAGCGTTATCAACACCTTACCAAATGTTACC
ATCACCGTTGCTGGTCGTGAATTTGTTTTAACTCCAAAAGAATACGTTTTAGAAGTTACT
GAGTTCGGAAAGACTGAATGTTTGAGTGGATTTATGGGTATCGAGTTAAACATGGGAAAT
TTCTGGATCCTTGGTGATGTTTTCATCTCTGCTTACTATACTGTATTCGATTTTGGTAAT
AAACAAGTGGTTTCGCAACTGCCATTCAAGGTNAATTTTTT
sequence update 2001. 6. 9
Translated Amino Acid sequence 
vt*lt*KKMKLLILTLFLATIVLAQALTVPLNFHQASRESRRRVPQKWSNRLSALNAGTT
IPISDFEDAQYYGAITIGTPGQAFKVVFDTGSSNLWIPSKKCPITVVACDLHNKYNSGAS
STYVANGTDFTIQYGSGAMSGFVSQDSVTVGSLTVKDQLFAEATAEPGIAFDFAKFDGIL
GLAFQSISVNSIPPVFYNMLSQGLVSSTLFSFWLSRTPGANGGELSFGSIDNTKYTGDIT
YVPLTNETYWEFVMDDFAIDGQSAGFCGTTCHAICDSGTSLIAGPMADITALNEKLGAVI
LNGEGVFSDCSVINTLPNVTITVAGREFVLTPKEYVLEVTEFGKTECLSGFMGIELNMGN
FWILGDVFISAYYTVFDFGNKQVVSQLPFKVNF
Translated Amino Acid sequence (All Frames) 
Frame A:
vt*lt*KKMKLLILTLFLATIVLAQALTVPLNFHQASRESRRRVPQKWSNRLSALNAGTT
IPISDFEDAQYYGAITIGTPGQAFKVVFDTGSSNLWIPSKKCPITVVACDLHNKYNSGAS
STYVANGTDFTIQYGSGAMSGFVSQDSVTVGSLTVKDQLFAEATAEPGIAFDFAKFDGIL
GLAFQSISVNSIPPVFYNMLSQGLVSSTLFSFWLSRTPGANGGELSFGSIDNTKYTGDIT
YVPLTNETYWEFVMDDFAIDGQSAGFCGTTCHAICDSGTSLIAGPMADITALNEKLGAVI
LNGEGVFSDCSVINTLPNVTITVAGREFVLTPKEYVLEVTEFGKTECLSGFMGIELNMGN
FWILGDVFISAYYTVFDFGNKQVVSQLPFKVNF


Frame B:
spdlhkrk*nylf*lyf*lllf*lkl*qyh*tsiklqenleeefhkngqtdyllsmlvpq
sqfqilkmlntmvplplvpqvkpsk*fsilvhptcgfhqrnvqslllhviyitnitavpq
ahmsptelispsntvvvlcqvlslkipslxvh*llkinyslkplpnqvllsispnsmvf*
vllsnpslliqfhqsfttcyhkv*fhqhsspsgyqelqvptvvnshsvqsitpntlvtlp
tsh*ptkpignslwmtllsmvnqlvsvvllvtqfaiqvhhsllvqwlillpsmkn*vlss
*mvkvsslivalstpyqmlpspllvvnlf*lqkntf*kllsserlnv*vdlwvss*twei
sgslvmfsslltilysilvinkwfrnchsrxif


Frame C:
hltyikeneityfnfifsyycfsssfnstiklpssfkri*kksstkmvkqiicsqcwyhn
pnfrf*rcsilwchyhwyprsslqssfrywfiqlvdsikemsnhcccm*ft*qi*qrclk
hicrqrn*fhhpir*wcyvrfclsrfrhcxfinc*rsiir*shcrtrycfrfrqirwyfr
scfpihlc*fnstsllqhvitrfsfintllllviknsrcqrw*tlirfnr*hqihw*hyl
rpinqrnllgiryg*lcyrwsiswflwyylsrnlrfryithcwsng*yycpq*kircchl
kw*rcll*l*ryqhltkcyhhrcws*icfnskrirfrsy*vrkd*mfewiygyrvkhgkf
ldpw*cfhlcllycirfw**tsgfataiqgxff
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFB569 (VFB569Q) /CSM/VF/VFB5-C/VFB569Q.Seq.d/                       2329   0.0
VFO689 (VFO689Q) /CSM/VF/VFO6-D/VFO689Q.Seq.d/                       2315   0.0
VFB255 (VFB255Q) /CSM/VF/VFB2-C/VFB255Q.Seq.d/                       2315   0.0
VFB231 (VFB231Q) /CSM/VF/VFB2-B/VFB231Q.Seq.d/                       2315   0.0
VFA814 (VFA814Q) /CSM/VF/VFA8-A/VFA814Q.Seq.d/                       2315   0.0
VFO734 (VFO734Q) /CSM/VF/VFO7-B/VFO734Q.Seq.d/                       2313   0.0
VFA146 (VFA146Q) /CSM/VF/VFA1-B/VFA146Q.Seq.d/                       2313   0.0
VFN222 (VFN222Q) /CSM/VF/VFN2-A/VFN222Q.Seq.d/                       2307   0.0
VFM881 (VFM881Q) /CSM/VF/VFM8-D/VFM881Q.Seq.d/                       2307   0.0
VFM519 (VFM519Q) /CSM/VF/VFM5-A/VFM519Q.Seq.d/                       2307   0.0
own update 2004. 8. 9
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

Y16962|Y16962.1 Dictyostelium discoideum mRNA for cathepsin D.  2264  0.0  2
AJ243946|AJ243946.1 Dictyostelium discoideum ctsD gene for cathepsin D, exons 1 to 2.  2105  0.0  3
E33916|E33916.1 Candida boidinii strain with lowered protease activity and  utilization thereof as host for producing foreign protein.    36  8e-07  5
AL161500|AL161500.2 Arabidopsis thaliana DNA chromosome 4, contig fragment No. 12.    62  2e-05  1
AL762930|AL762930.1 Arabidopsis thaliana T-DNA flanking sequence GK-030C11-011522.    62  2e-05  1
AV567523|AV567523.1 Arabidopsis thaliana cDNA clone:SQL15g03F, 3' end.    62  2e-05  1
AF372974|AF372974.1 Arabidopsis thaliana AT4g04460/T26N6_7 mRNA, complete cds.    62  2e-05  1
CB264640|CB264640.1 48-E014661-035-002-P12-T7R MPIZ-ADIS-035 Arabidopsis thaliana cDNA clone MPIZp2000P122Q 5-PRIME, mRNA sequence.    62  2e-05  1
BE525959|BE525959.1 M64C07STM Arabidopsis developing seed Arabidopsis thaliana cDNA clone 600034526R1 5', mRNA sequence.    62  2e-05  1
CD825453|CD825453.1 BN25.060N03F011129 BN25 Brassica napus cDNA clone BN25060N03, mRNA sequence.    62  2e-05  1
dna update 2003. 9. 3
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

EF213114_1(EF213114|pid:none) Penaeus monodon cathepsin D mRNA, ...   369   e-101
(Q03168) RecName: Full=Lysosomal aspartic protease;          EC=...   369   e-100
BT080419_1(BT080419|pid:none) Caligus clemensi clone ccle-evs-50...   368   e-100
DQ010007_1(DQ010007|pid:none) Bombyx mori CathD mRNA, complete c...   367   e-100
EF070454_1(EF070454|pid:none) Maconellicoccus hirsutus clone WHM...   367   e-100
AB106552_1(AB106552|pid:none) Todarodes pacificus tpaD mRNA for ...   360   4e-98
DQ909010_1(DQ909010|pid:none) Clonorchis sinensis aspartic prote...   358   2e-97
FJ654712_1(FJ654712|pid:none) Chrysomela tremulae aspartic prote...   358   3e-97
FJ168036_1(FJ168036|pid:none) Fasciola hepatica cathepsin D-like...   357   5e-97
EF000001_1(EF000001|pid:none) Fasciola hepatica cathepsin D-like...   349   9e-95
protein update 2009. 7.10
PSORT 

 psg: 0.98  gvh: 0.77  alm: 0.41  top: 0.57  tms: 0.00  mit: 0.36  mip: 0.08
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 28.0 %: extracellular, including cell wall
	 24.0 %: mitochondrial
	 12.0 %: vacuolar
	 12.0 %: nuclear
	  8.0 %: cytoplasmic
	  8.0 %: Golgi
	  8.0 %: endoplasmic reticulum

>> prediction for VFB569 is exc
5' end seq. ID VFB569F
5' end seq. 
>VFB569F.Seq
GTCACCTGACTTACATAAAAGAAAATGAAATTACTTATTTTAACTTTATTTTTAGCTACT
ATTGTTTTAGCTCAAGCTTTAACAGTACCATTAAACTTCCATCAAGCTTCAAGAGAATCT
AGAAGAAGAGTTCCACAAAAATGGTCAAACAGATTATCTGCTCTCAATGCTGGTACCACA
ATCCCAATTTCAGATTTTGAAGATGCTCAATACTATGGTGCCATTACCATTGGTACCCCA
GGTCAAGCCTTCAAAGTAGTTTTCGATACTGGTTCATCCAACTTGTGGATTCCATCAAAG
AAATGTCCAATCACTGTTGTTGCATGTGATTTACATAACAAATATAACAGCGGTGCCTCA
AGCACATATGTCGCCAACGGAACTGATTTCACCATCCAATACGGTAGTGGTGCTATGTCA
GGTTTTGTCTCTCAAGATTCCGTCACTGTTGGTTCATTAACTGTTAAAGATCAATTATTC
GCTGAAGCCACTGCCGAACCAGGTATTGCTTTCGATTT----------
Length of 5' end seq. 518
3' end seq. ID VFB569Z
3' end seq. 
>VFB569Z.Seq
----------TTCCGTCACTGTGGGTTCATTAACTGTTAAAGATCAATTATTCGCTGAAG
CCACTGCCGAACCAGGTATTGCTTTCGATTTCGCCAAATTCGATGGTATTTTAGGTCTTG
CTTTCCAATCCATCTCTGTTAATTCAATTCCACCAGTCTTTTACAACATGTTATCACAAG
GTTTAGTTTCATCAACACTCTTCTCCTTCTGGTTATCAAGAACTCCAGGTGCCAACGGTG
GTGAACTCTCATTCGGTTCAATCGATAACACCAAATACACTGGTGACATTACCTACGTCC
CATTAACCAACGAAACCTATTGGGAATTCGTTATGGATGACTTTGCTATCGATGGTCAAT
CAGCTGGTTTCTGTGGTACTACTTGTCACGCAATTTGCGATTCAGGTACATCACTCATTG
CTGGTCCAATGGCTGATATTACTGCCCTCAATGAAAAATTAGGTGCTGTCATCTTAAATG
GTGAAGGTGTCTTCTCTGATTGTAGCGTTATCAACACCTTACCAAATGTTACCATCACCG
TTGCTGGTCGTGAATTTGTTTTAACTCCAAAAGAATACGTTTTAGAAGTTACTGAGTTCG
GAAAGACTGAATGTTTGAGTGGATTTATGGGTATCGAGTTAAACATGGGAAATTTCTGGA
TCCTTGGTGATGTTTTCATCTCTGCTTACTATACTGTATTCGATTTTGGTAATAAACAAG
TGGTTTCGCAACTGCCATTCAAGGTNAATTTTTT
Length of 3' end seq. 744
Connected seq. ID VFB569P
Connected seq. 
>VFB569P.Seq
GTCACCTGACTTACATAAAAGAAAATGAAATTACTTATTTTAACTTTATTTTTAGCTACT
ATTGTTTTAGCTCAAGCTTTAACAGTACCATTAAACTTCCATCAAGCTTCAAGAGAATCT
AGAAGAAGAGTTCCACAAAAATGGTCAAACAGATTATCTGCTCTCAATGCTGGTACCACA
ATCCCAATTTCAGATTTTGAAGATGCTCAATACTATGGTGCCATTACCATTGGTACCCCA
GGTCAAGCCTTCAAAGTAGTTTTCGATACTGGTTCATCCAACTTGTGGATTCCATCAAAG
AAATGTCCAATCACTGTTGTTGCATGTGATTTACATAACAAATATAACAGCGGTGCCTCA
AGCACATATGTCGCCAACGGAACTGATTTCACCATCCAATACGGTAGTGGTGCTATGTCA
GGTTTTGTCTCTCAAGATTCCGTCACTGTTGGTTCATTAACTGTTAAAGATCAATTATTC
GCTGAAGCCACTGCCGAACCAGGTATTGCTTTCGATTT----------TTCCGTCACTGT
GGGTTCATTAACTGTTAAAGATCAATTATTCGCTGAAGCCACTGCCGAACCAGGTATTGC
TTTCGATTTCGCCAAATTCGATGGTATTTTAGGTCTTGCTTTCCAATCCATCTCTGTTAA
TTCAATTCCACCAGTCTTTTACAACATGTTATCACAAGGTTTAGTTTCATCAACACTCTT
CTCCTTCTGGTTATCAAGAACTCCAGGTGCCAACGGTGGTGAACTCTCATTCGGTTCAAT
CGATAACACCAAATACACTGGTGACATTACCTACGTCCCATTAACCAACGAAACCTATTG
GGAATTCGTTATGGATGACTTTGCTATCGATGGTCAATCAGCTGGTTTCTGTGGTACTAC
TTGTCACGCAATTTGCGATTCAGGTACATCACTCATTGCTGGTCCAATGGCTGATATTAC
TGCCCTCAATGAAAAATTAGGTGCTGTCATCTTAAATGGTGAAGGTGTCTTCTCTGATTG
TAGCGTTATCAACACCTTACCAAATGTTACCATCACCGTTGCTGGTCGTGAATTTGTTTT
AACTCCAAAAGAATACGTTTTAGAAGTTACTGAGTTCGGAAAGACTGAATGTTTGAGTGG
ATTTATGGGTATCGAGTTAAACATGGGAAATTTCTGGATCCTTGGTGATGTTTTCATCTC
TGCTTACTATACTGTATTCGATTTTGGTAATAAACAAGTGGTTTCGCAACTGCCATTCAA
GGTNAATTTTTT
Length of connected seq. 1262
Full length Seq ID VFB569E
Full length Seq. 
>VFB569E.Seq
GTCACCTGACTTACATAAAAGAAAATGAAATTACTTATTTTAACTTTATTTTTAGCTACT
ATTGTTTTAGCTCAAGCTTTAACAGTACCATTAAACTTCCATCAAGCTTCAAGAGAATCT
AGAAGAAGAGTTCCACAAAAATGGTCAAACAGATTATCTGCTCTCAATGCTGGTACCACA
ATCCCAATTTCAGATTTTGAAGATGCTCAATACTATGGTGCCATTACCATTGGTACCCCA
GGTCAAGCCTTCAAAGTAGTTTTCGATACTGGTTCATCCAACTTGTGGATTCCATCAAAG
AAATGTCCAATCACTGTTGTTGCATGTGATTTACATAACAAATATAACAGCGGTGCCTCA
AGCACATATGTCGCCAACGGAACTGATTTCACCATCCAATACGGTAGTGGTGCTATGTCA
GGTTTTGTCTCTCAAGATTCCGTCACTGTNGGTTCATTAACTGTTAAAGATCAATTATTC
GCTGAAGCCACTGCCGAACCAGGTATTGCTTTCGATTTCGCCAAATTCGATGGTATTTTA
GGTCTTGCTTTCCAATCCATCTCTGTTAATTCAATTCCACCAGTCTTTTACAACATGTTA
TCACAAGGTTTAGTTTCATCAACACTCTTCTCCTTCTGGTTATCAAGAACTCCAGGTGCC
AACGGTGGTGAACTCTCATTCGGTTCAATCGATAACACCAAATACACTGGTGACATTACC
TACGTCCCATTAACCAACGAAACCTATTGGGAATTCGTTATGGATGACTTTGCTATCGAT
GGTCAATCAGCTGGTTTCTGTGGTACTACTTGTCACGCAATTTGCGATTCAGGTACATCA
CTCATTGCTGGTCCAATGGCTGATATTACTGCCCTCAATGAAAAATTAGGTGCTGTCATC
TTAAATGGTGAAGGTGTCTTCTCTGATTGTAGCGTTATCAACACCTTACCAAATGTTACC
ATCACCGTTGCTGGTCGTGAATTTGTTTTAACTCCAAAAGAATACGTTTTAGAAGTTACT
GAGTTCGGAAAGACTGAATGTTTGAGTGGATTTATGGGTATCGAGTTAAACATGGGAAAT
TTCTGGATCCTTGGTGATGTTTTCATCTCTGCTTACTATACTGTATTCGATTTTGGTAAT
AAACAAGTGGTTTCGCAACTGCCATTCAAGGTNAATTTTTT
Length of full length seq. 1181