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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFB534
Library VF
(Link to library)
Clone ID VFB534
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15835-1
Original site URL
Representative seq. ID VFB534P
(Link to Original site)
Representative DNA sequence 
>VFB534 (VFB534Q) /CSM/VF/VFB5-B/VFB534Q.Seq.d/
AATCATCGAAAGTATTTTACAGATAATATAAAATGTCTTCCTCTATTGGTATTGATTTAG
GTACAACTTACTCATGTGTTGGTGTCTGGCAAAATGATCGTGTTGAAATCATTGCTAATG
ATCAAGGTAACAGAACCACCCCATCATACGTTGCTTTCAACGAAACTGAACGTTTAATTG
GTGATGCTGCTAAGAATCAAGTTGCCATGAATCCAACCAACACCATCTTTGATGCCAAAC
GTTTAATTGGTCGTAAATTCTCTGATCCAGTTATCCAATCCGATATGAAACATTGGCCAT
TCAAAGTTATCGCCAAAGAAGGTGACAAACCACATCTCCAAGTTGAATTCAAAGGTGAAG
TTAAAACTTTCTCACCAGAAGAAGTCTCTTCAATGGTTCTCTTAAAGATGAAAGAAACCG
CTGAAGCTTACCTCGGTAAAACCATCAACAACGCCGTTATCACCGTTCCAGCTTATTTCA
ACGATAGCCAACGTCAAGCCACCAAAGATGCAGGTGCCATCGCXXXXXXXXXXAGTTACT
TTCNATATNGATGCCAACGGTATCTTAAATGTCTCTGCTAAAGATAAGAGCACTGGTAAC
AAACACAAAATCACCATCACCAACGATAAAGGTAGACTCACTGCTGAACAAATCGAAAAG
ATGGTCAAAGATGCTGAAATGTTCAAAGCTCAAGATGAAGCCCAACGTGAAGTTGTTGAA
TCAAAGAACAAATTAGAAAACTACGCTTACACCGTTCGTAGCACCATTAAAGACGATAAG
ATCGCCGCCAAATTATCAAAAGAAGATCGTAAGACCGTCGAAGAGAAATCCGATGAAGCC
ATCAACTGGTTACATGCCAACGACTCTGCCACTAAAGAAGAATACGAAAAAGCCATGAAA
GATTTAGAAGCTGTTTGTTCACCAATCATCTCTAAAGTTTACGGTCAACAAGGTGGTAAA
ACCCAGGTACTTCTCTGGTGCCAAAACCACTAAGAAGATT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
sskvfyr*YKMSSSIGIDLGTTYSCVGVWQNDRVEIIANDQGNRTTPSYVAFNETERLIG
DAAKNQVAMNPTNTIFDAKRLIGRKFSDPVIQSDMKHWPFKVIAKEGDKPHLQVEFKGEV
KTFSPEEVSSMVLLKMKETAEAYLGKTINNAVITVPAYFNDSQRQATKDAGAI---

---VTFXXDANGILNVSAKDKSTGNKHKITITNDKGRLTAEQIEKMVKDAEMFKAQDEAQ
REVVESKNKLENYAYTVRSTIKDDKIAAKLSKEDRKTVEEKSDEAINWLHANDSATKEEY
EKAMKDLEAVCSPIISKVYGQQGGKTQVLLWCQNH*ed
Translated Amino Acid sequence (All Frames) 
Frame A:
nhrkyftdnikclpllvli*vqlthvlvsgkmivlksllmikvtepphhtllstklnv*l
vmllriklp*iqptpslmpnv*lvvnsliqlsnpi*nighsklspkkvtnhisklnskvk
lklshqkkslqwfs*r*kkplkltsvkpsttplspfqlistianvkppkmqvps---

---syfxyxcqrylkclc*r*ehw*qtqnhhhqr*r*thc*tnrkdgqrc*nvqssr*sp
t*sc*ikeqirklrlhrs*hh*rr*drrqiikrrs*drrreir*shqlvtcqrlch*rri
rksherfrsclftnhl*slrstrw*npgtslvpkplrr

Frame B:
iiesilqii*nvflywy*frynllmcwclak*sc*nhc**sr*qnhpiircfqrn*tfnw
*cc*esschesnqhhl*cqtfnws*il*ssypiryetlaiqsyrqrr*qttsps*iqr*s
*nfltrrslfngslkdernr*slpr*nhqqrryhrsslfqr*ptsshqrcrchr---

---VTFXXDANGILNVSAKDKSTGNKHKITITNDKGRLTAEQIEKMVKDAEMFKAQDEAQ
REVVESKNKLENYAYTVRSTIKDDKIAAKLSKEDRKTVEEKSDEAINWLHANDSATKEEY
EKAMKDLEAVCSPIISKVYGQQGGKTQVLLWCQNH*ed

Frame C:
sskvfyr*YKMSSSIGIDLGTTYSCVGVWQNDRVEIIANDQGNRTTPSYVAFNETERLIG
DAAKNQVAMNPTNTIFDAKRLIGRKFSDPVIQSDMKHWPFKVIAKEGDKPHLQVEFKGEV
KTFSPEEVSSMVLLKMKETAEAYLGKTINNAVITVPAYFNDSQRQATKDAGAI---

---llsixmptvs*msllkiralvtntkspsptikvdsllnkskrwskmlkcsklkmkpn
vkllnqrtn*kttltpfvaplktirsppnyqkkivrpskrnpmkpstgympttlplkknt
kkp*ki*klfvhqsslkftvnkvvkpryfsgakttkki
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFB534 (VFB534Q) /CSM/VF/VFB5-B/VFB534Q.Seq.d/                       1911   0.0
VHL592 (VHL592Q) /CSM/VH/VHL5-D/VHL592Q.Seq.d/                       1037   0.0
VHI852 (VHI852Q) /CSM/VH/VHI8-C/VHI852Q.Seq.d/                       1037   0.0
VHI539 (VHI539Q) /CSM/VH/VHI5-B/VHI539Q.Seq.d/                       1037   0.0
VHH730 (VHH730Q) /CSM/VH/VHH7-B/VHH730Q.Seq.d/                       1037   0.0
VHG386 (VHG386Q) /CSM/VH/VHG3-D/VHG386Q.Seq.d/                       1037   0.0
VHE585 (VHE585Q) /CSM/VH/VHE5-D/VHE585Q.Seq.d/                       1037   0.0
VHC434 (VHC434Q) /CSM/VH/VHC4-B/VHC434Q.Seq.d/                       1037   0.0
VFG352 (VFG352Q) /CSM/VF/VFG3-C/VFG352Q.Seq.d/                       1037   0.0
VFE160 (VFE160Q) /CSM/VF/VFE1-C/VFE160Q.Seq.d/                       1037   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AF025951|AF025951.1 Dictyostelium discoideum heat-shock cognate protein 70 (hsc70) mRNA, complete cds.  1037  0.0  3
AC115684|AC115684.2 Dictyostelium discoideum chromosome 2 map 3108975-3191114 strain AX4, complete sequence.   991  0.0  4
X75263|X75263.1 D.discoideum (AX3) mRNA for heat shock cognate protein (hsc70).   252  e-138  5
L22736|L22736.1 Dictyostelium discoideum heat shock protein (hspB) gene,.   242  e-100  6
AF188288|AF188288.2 Rhizopus stolonifer Hsp70 protein 1 mRNA, complete cds.   153  4e-64  6
AY147869|AY147869.1 Rhizopus stolonifer 70 kDa heat shock protein 3 gene, complete cds.   155  2e-62  7
AY147871|AY147871.1 Rhizopus stolonifer 70 kDa heat shock protein 1 gene, partial cds.   153  8e-58  6
AJ238887|AJ238887.1 Piromyces sp. E2 hsp70.2 gene for putative heat shock protein 70,  partial.   165  6e-54  4
AX536934|AX536934.1 Sequence 535 from Patent WO02064766.         107  4e-50  5
A58313|A58313.1 Sequence 1 from Patent WO9636707.                107  5e-50  5
dna update 2003. 9. 3
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q557E0) RecName: Full=Heat shock cognate 70 kDa protein 2;     ...   329   1e-88
AF025951_1(AF025951|pid:none) Dictyostelium discoideum heat-shoc...   329   1e-88
(P36415) RecName: Full=Heat shock cognate 70 kDa protein 1;     ...   305   1e-81
S37394(S37394) dnaK-type molecular chaperone hsc70 - slime mold ...   305   1e-81
AY495382_1(AY495382|pid:none) Trypanosoma rangeli isolate Tre he...   286   8e-76
M26595_1(M26595|pid:none) T.cruzi heat shock protein (HSP70) gen...   285   2e-75
(Q54BE0) RecName: Full=Heat shock cognate 70 kDa protein 3;           285   2e-75
EF442767_1(EF442767|pid:none) Trypanosoma rangeli isolate H14 cl...   284   4e-75
FJ222459_1(FJ222459|pid:none) Trypanosoma cruzi heat shock prote...   283   5e-75
U71151_1(U71151|pid:none) Pneumocystis carinii heat shock protei...   282   1e-74
protein update 2009. 6.17
PSORT 

 psg: 0.87  gvh: 0.35  alm: 0.45  top: 0.27  tms: 0.00  mit: 0.20  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 32.0 %: nuclear
	  8.0 %: vacuolar
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for VFB534 is cyt
5' end seq. ID VFB534F
5' end seq. 
>VFB534F.Seq
AATCATCGAAAGTATTTTACAGATAATATAAAATGTCTTCCTCTATTGGTATTGATTTAG
GTACAACTTACTCATGTGTTGGTGTCTGGCAAAATGATCGTGTTGAAATCATTGCTAATG
ATCAAGGTAACAGAACCACCCCATCATACGTTGCTTTCAACGAAACTGAACGTTTAATTG
GTGATGCTGCTAAGAATCAAGTTGCCATGAATCCAACCAACACCATCTTTGATGCCAAAC
GTTTAATTGGTCGTAAATTCTCTGATCCAGTTATCCAATCCGATATGAAACATTGGCCAT
TCAAAGTTATCGCCAAAGAAGGTGACAAACCACATCTCCAAGTTGAATTCAAAGGTGAAG
TTAAAACTTTCTCACCAGAAGAAGTCTCTTCAATGGTTCTCTTAAAGATGAAAGAAACCG
CTGAAGCTTACCTCGGTAAAACCATCAACAACGCCGTTATCACCGTTCCAGCTTATTTCA
ACGATAGCCAACGTCAAGCCACCAAAGATGCAGGTGCCATCGC----------
Length of 5' end seq. 523
3' end seq. ID VFB534Z
3' end seq. 
>VFB534Z.Seq
----------AGTTACTTTCNATATNGATGCCAACGGTATCTTAAATGTCTCTGCTAAAG
ATAAGAGCACTGGTAACAAACACAAAATCACCATCACCAACGATAAAGGTAGACTCACTG
CTGAACAAATCGAAAAGATGGTCAAAGATGCTGAAATGTTCAAAGCTCAAGATGAAGCCC
AACGTGAAGTTGTTGAATCAAAGAACAAATTAGAAAACTACGCTTACACCGTTCGTAGCA
CCATTAAAGACGATAAGATCGCCGCCAAATTATCAAAAGAAGATCGTAAGACCGTCGAAG
AGAAATCCGATGAAGCCATCAACTGGTTACATGCCAACGACTCTGCCACTAAAGAAGAAT
ACGAAAAAGCCATGAAAGATTTAGAAGCTGTTTGTTCACCAATCATCTCTAAAGTTTACG
GTCAACAAGGTGGTAAAACCCAGGTACTTCTCTGGTGCCAAAACCACTAAGAAGATT
Length of 3' end seq. 467
Connected seq. ID VFB534P
Connected seq. 
>VFB534P.Seq
AATCATCGAAAGTATTTTACAGATAATATAAAATGTCTTCCTCTATTGGTATTGATTTAG
GTACAACTTACTCATGTGTTGGTGTCTGGCAAAATGATCGTGTTGAAATCATTGCTAATG
ATCAAGGTAACAGAACCACCCCATCATACGTTGCTTTCAACGAAACTGAACGTTTAATTG
GTGATGCTGCTAAGAATCAAGTTGCCATGAATCCAACCAACACCATCTTTGATGCCAAAC
GTTTAATTGGTCGTAAATTCTCTGATCCAGTTATCCAATCCGATATGAAACATTGGCCAT
TCAAAGTTATCGCCAAAGAAGGTGACAAACCACATCTCCAAGTTGAATTCAAAGGTGAAG
TTAAAACTTTCTCACCAGAAGAAGTCTCTTCAATGGTTCTCTTAAAGATGAAAGAAACCG
CTGAAGCTTACCTCGGTAAAACCATCAACAACGCCGTTATCACCGTTCCAGCTTATTTCA
ACGATAGCCAACGTCAAGCCACCAAAGATGCAGGTGCCATCGC----------AGTTACT
TTCNATATNGATGCCAACGGTATCTTAAATGTCTCTGCTAAAGATAAGAGCACTGGTAAC
AAACACAAAATCACCATCACCAACGATAAAGGTAGACTCACTGCTGAACAAATCGAAAAG
ATGGTCAAAGATGCTGAAATGTTCAAAGCTCAAGATGAAGCCCAACGTGAAGTTGTTGAA
TCAAAGAACAAATTAGAAAACTACGCTTACACCGTTCGTAGCACCATTAAAGACGATAAG
ATCGCCGCCAAATTATCAAAAGAAGATCGTAAGACCGTCGAAGAGAAATCCGATGAAGCC
ATCAACTGGTTACATGCCAACGACTCTGCCACTAAAGAAGAATACGAAAAAGCCATGAAA
GATTTAGAAGCTGTTTGTTCACCAATCATCTCTAAAGTTTACGGTCAACAAGGTGGTAAA
ACCCAGGTACTTCTCTGGTGCCAAAACCACTAAGAAGATT
Length of connected seq. 990
Full length Seq ID -
Full length Seq. -
Length of full length seq. -