>VFB533 (VFB533Q) /CSM/VF/VFB5-B/VFB533Q.Seq.d/
CACTGTTGGCCTACTGGNATTTTCTTATACTTTTAACAAAAAGAAATAATAGAGAAAAAG
GGAAAAACTTTTTTTTTAATAAAAAAATAAAATAAAAATAAAAATTTAATCCCTGTTATG
ATTAAAAGCGTTAAGTTATTAAAAACCATCTCAAAAACAAGTTTTATTTGTAATAATAAT
AAATTTATAGGAAATTATAATAATAATAATTTTATAAGAAAAATGAGTACCACAAATACA
AATACTGCAAATAGTAGTAATAATAATAATAATAATAATAATAATCAAAAACAAAAACAA
CAACAAAAACCAAAAAATAATGATAAAGGATGTTGTGATCCAAGTGTTAATCAACCAAAA
TCTAAACAAAAACCAATTGATAATAGTAAAGCAAAATTGAATACACCAAAAGTTGTTGAT
ATGGCGGAGGATGCAAAATATGTTGATTCACATGTACATGTTGACCAACTTTTAATAAGA
TTAAATAAATCATTAACAGATTTCCCACAATTTAAACAAXXXXXXXXXXAATTAATTCAA
GTTTGTTGTGATCCAGTATCGATAGAATATGCAGATTTCTTAATTGAAAACAATGAGTGT
ATCTATGGTGCCTATGGCGTCCACCCTCACAATGCCAAGGAATATAATGACCAAATTGAG
CAAAAACTAATAGACAGGATGAAATCAAATCCAAAAGTTGTGGCATGGGGCGAGATGGGG
TTAGACTATTTTTACAATAAATCTTCAAATGACGAGCAAATCAATGCCTTCTCACGTCAA
TTGAAGCAGGCTGTCAAGTTGGGTAAACCATTGGTAATCCATAGTCGTGAGGCTGAGGAG
GACACATTGCGTCTCTTGAAGGAGTTGGTACCTGTTGATTATAAAATTCATATCCATTGT
TTCACGTCCAATAGTGATTTCGCAAAACAATTACTTGACCATTTCCCAAATCTTTGTATT
GGGTTCACTGGTTGCATCACTTTTAGAAACTCTCAATCAATCAGAGATAGCGTTGAAGCC
GTGCCAATCGAACGTATCCTTTTGGAAACTGATGGTCCATATATGACACCTGAACCATTC
CGTGGTCAAATTGCTCATTCTGGGCACATTCCAATGGTTGCAAATGCAATCTCTCAAATT
AAAAATATACCCCTAGATCAAGTTTTAAAACAATGTAGAGAAAATACTACTAAAATTTAG
GAATTTAAAAATAAAATAAAATCAAA

hcwptgiflyf*qkeiiekkgktfflikk*NKNKNLIPVMIKSVKLLKTISKTSFICNNN
KFIGNYNNNNFIRKMSTTNTNTANSSNNNNNNNNNQKQKQQQKPKNNDKGCCDPSVNQPK
SKQKPIDNSKAKLNTPKVVDMAEDAKYVDSHVHVDQLLIRLNKSLTDFPQFKQ---

---LIQVCCDPVSIEYADFLIENNECIYGAYGVHPHNAKEYNDQIEQKLIDRMKSNPKVV
AWGEMGLDYFYNKSSNDEQINAFSRQLKQAVKLGKPLVIHSREAEEDTLRLLKELVPVDY
KIHIHCFTSNSDFAKQLLDHFPNLCIGFTGCITFRNSQSIRDSVEAVPIERILLETDGPY
MTPEPFRGQIAHSGHIPMVANAISQIKNIPLDQVLKQCRENTTKI*efknkiks

Frame A:
hcwptgiflyf*qkeiiekkgktfflikk*NKNKNLIPVMIKSVKLLKTISKTSFICNNN
KFIGNYNNNNFIRKMSTTNTNTANSSNNNNNNNNNQKQKQQQKPKNNDKGCCDPSVNQPK
SKQKPIDNSKAKLNTPKVVDMAEDAKYVDSHVHVDQLLIRLNKSLTDFPQFKQ---

---n*fkfvviqyr*nmqis*lktmsvsmvpmastltmprnimtklskn**tg*nqiqkl
whgarwg*tiftinlqmtsksmpshvn*srlsswvnhw*sivvrlrrthcvs*rswylli
ikfisivsrpivisqnnyltisqifvlgslvaslletlnqseialkpcqsnvsfwklmvh
i*hlnhsvvkllilgtfqwlqmqslklkiyp*ikf*nnvekillkfrnlkik*nq

Frame B:
tvgllxfsytfnkkk**rkreklff**knkikiki*sll*lkalsy*kpsqkqvlfviii
nl*eiiiiiil*ek*vpqiqilqivviiiiiiiiiknknnnknqkimikdvviqvlinqn
lnknqliivkqn*ihqklliwrrmqnmlihmymltnf**d*inh*qishnln---

---inssll*ssidricrfln*kq*vylwclwrppsqcqgi**pn*aktnrqdeiksksc
gmgrdgvrlflq*ifk*ranqclltsieagcqvg*tignp*s*g*gghiaslegvgtc*l
*nsyplfhvq**frktit*pfpkslywvhwlhhf*klsinqr*r*sranrtypfgn*wsi
ydt*tipwsncsfwahsngckcnlsn*kytprssfktm*rkyy*nlgi*k*nkik

Frame C:
llaywxflilltkrnnrekgknfffnkkik*k*kfnpcyd*kr*viknhlknkfyl****
iyrkl****fykkneyhkykyck***********sktktttktkk***rml*skc*stki
*tktn****skieytksc*ygggckic*ftctc*ptfnkik*iinrfpti*t---

---LIQVCCDPVSIEYADFLIENNECIYGAYGVHPHNAKEYNDQIEQKLIDRMKSNPKVV
AWGEMGLDYFYNKSSNDEQINAFSRQLKQAVKLGKPLVIHSREAEEDTLRLLKELVPVDY
KIHIHCFTSNSDFAKQLLDHFPNLCIGFTGCITFRNSQSIRDSVEAVPIERILLETDGPY
MTPEPFRGQIAHSGHIPMVANAISQIKNIPLDQVLKQCRENTTKI*efknkiks

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFB533 (VFB533Q) /CSM/VF/VFB5-B/VFB533Q.Seq.d/                       1651   0.0
VFK114 (VFK114Q) /CSM/VF/VFK1-A/VFK114Q.Seq.d/                       1275   0.0
VSA591 (VSA591Q) /CSM/VS/VSA5-D/VSA591Q.Seq.d/                        866   0.0
AHD255 (AHD255Q) /CSM/AH/AHD2-C/AHD255Q.Seq.d/                         46   0.001
VFL457 (VFL457Q) /CSM/VF/VFL4-C/VFL457Q.Seq.d/                         44   0.005
VFB674 (VFB674Q) /CSM/VF/VFB6-D/VFB674Q.Seq.d/                         44   0.005
SFI470 (SFI470Q) /CSM/SF/SFI4-C/SFI470Q.Seq.d/                         44   0.005
AHM117 (AHM117Q) /CSM/AH/AHM1-A/AHM117Q.Seq.d/                         44   0.005
VHI578 (VHI578Q) /CSM/VH/VHI5-D/VHI578Q.Seq.d/                         42   0.020
VFM201 (VFM201Q) /CSM/VF/VFM2-A/VFM201Q.Seq.d/                         42   0.020

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AX346049|AX346049.1 Sequence 1120 from Patent WO0200928.          40  1.0  2
AC125021|AC125021.3 Mus musculus clone RP24-212P5, WORKING DRAFT SEQUENCE, 5 unordered pieces.    46  1.1  1
AC110397|AC110397.4 Rattus norvegicus clone CH230-87E18, *** SEQUENCING IN PROGRESS ***, 1 ordered piece.    46  1.1  1
AC125017|AC125017.3 Mus musculus clone RP24-157E23, WORKING DRAFT SEQUENCE, 35 unordered pieces.    46  1.1  1
AC097341|AC097341.5 Rattus norvegicus clone CH230-166G20, *** SEQUENCING IN PROGRESS ***, 9 unordered pieces.    44  4.1  1
AX346795|AX346795.1 Sequence 1866 from Patent WO0200928.          44  4.1  1
BQ802493|BQ802493.1 WHE2826_E08_I16ZS Triticum monococcum vernalized apex cDNA library Triticum monococcum cDNA clone WHE2826_E08_I16, mRNA sequence.    44  4.1  1
CD809206|CD809206.1 LcA05C14c Leymus chinensis leaf cDNA library Leymus chinensis cDNA clone LcA05C14, mRNA sequence.    44  4.1  1
X60113|X60113.1 D.guanche Adh and Adh-dup genes for alcohol dehydrogenase.    44  4.1  1
AC113804|AC113804.6 Rattus norvegicus clone CH230-293I19, WORKING DRAFT SEQUENCE, 2 unordered pieces.    42     16  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CP000595_230(CP000595|pid:none) Ostreococcus lucimarinus CCE9901...   205   2e-51
AK289794_1(AK289794|pid:none) Homo sapiens cDNA FLJ76748 complet...   187   9e-46
BC090935_1(BC090935|pid:none) Homo sapiens TatD DNase domain con...   185   2e-45
BC166026_1(BC166026|pid:none) Synthetic construct Mus musculus c...   185   3e-45
AK156197_1(AK156197|pid:none) Mus musculus activated spleen cDNA...   183   9e-45
BC162648_1(BC162648|pid:none) Danio rerio zgc:194021, mRNA (cDNA...   182   2e-44
AK146408_1(AK146408|pid:none) Mus musculus TIB-55 BB88 cDNA, RIK...   182   2e-44
BC134181_1(BC134181|pid:none) Danio rerio zgc:194021, mRNA (cDNA...   182   2e-44
CP001348_168(CP001348|pid:none) Clostridium cellulolyticum H10, ...   166   1e-39
AE008691_98(AE008691|pid:none) Thermoanaerobacter tengcongensis ...   157   6e-37

 psg: 0.77  gvh: 0.35  alm: 0.44  top: 0.53  tms: 0.00  mit: 0.43  mip: 0.08
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: mitochondrial
	 20.0 %: cytoplasmic
	 20.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: extracellular, including cell wall
	  4.0 %: peroxisomal

>> prediction for VFB533 is mit

>VFB533F.Seq
CACTGTTGGCCTACTGGNATTTTCTTATACTTTTAACAAAAAGAAATAATAGAGAAAAAG
GGAAAAACTTTTTTTTTAATAAAAAAATAAAATAAAAATAAAAATTTAATCCCTGTTATG
ATTAAAAGCGTTAAGTTATTAAAAACCATCTCAAAAACAAGTTTTATTTGTAATAATAAT
AAATTTATAGGAAATTATAATAATAATAATTTTATAAGAAAAATGAGTACCACAAATACA
AATACTGCAAATAGTAGTAATAATAATAATAATAATAATAATAATCAAAAACAAAAACAA
CAACAAAAACCAAAAAATAATGATAAAGGATGTTGTGATCCAAGTGTTAATCAACCAAAA
TCTAAACAAAAACCAATTGATAATAGTAAAGCAAAATTGAATACACCAAAAGTTGTTGAT
ATGGCGGAGGATGCAAAATATGTTGATTCACATGTACATGTTGACCAACTTTTAATAAGA
TTAAATAAATCATTAACAGATTTCCCACAATTTAAACAA----------

>VFB533Z.Seq
----------AATTAATTCAAGTTTGTTGTGATCCAGTATCGATAGAATATGCAGATTTC
TTAATTGAAAACAATGAGTGTATCTATGGTGCCTATGGCGTCCACCCTCACAATGCCAAG
GAATATAATGACCAAATTGAGCAAAAACTAATAGACAGGATGAAATCAAATCCAAAAGTT
GTGGCATGGGGCGAGATGGGGTTAGACTATTTTTACAATAAATCTTCAAATGACGAGCAA
ATCAATGCCTTCTCACGTCAATTGAAGCAGGCTGTCAAGTTGGGTAAACCATTGGTAATC
CATAGTCGTGAGGCTGAGGAGGACACATTGCGTCTCTTGAAGGAGTTGGTACCTGTTGAT
TATAAAATTCATATCCATTGTTTCACGTCCAATAGTGATTTCGCAAAACAATTACTTGAC
CATTTCCCAAATCTTTGTATTGGGTTCACTGGTTGCATCACTTTTAGAAACTCTCAATCA
ATCAGAGATAGCGTTGAAGCCGTGCCAATCGAACGTATCCTTTTGGAAACTGATGGTCCA
TATATGACACCTGAACCATTCCGTGGTCAAATTGCTCATTCTGGGCACATTCCAATGGTT
GCAAATGCAATCTCTCAAATTAAAAATATACCCCTAGATCAAGTTTTAAAACAATGTAGA
GAAAATACTACTAAAATTTAGGAATTTAAAAATAAAATAAAATCAAA

>VFB533P.Seq
CACTGTTGGCCTACTGGNATTTTCTTATACTTTTAACAAAAAGAAATAATAGAGAAAAAG
GGAAAAACTTTTTTTTTAATAAAAAAATAAAATAAAAATAAAAATTTAATCCCTGTTATG
ATTAAAAGCGTTAAGTTATTAAAAACCATCTCAAAAACAAGTTTTATTTGTAATAATAAT
AAATTTATAGGAAATTATAATAATAATAATTTTATAAGAAAAATGAGTACCACAAATACA
AATACTGCAAATAGTAGTAATAATAATAATAATAATAATAATAATCAAAAACAAAAACAA
CAACAAAAACCAAAAAATAATGATAAAGGATGTTGTGATCCAAGTGTTAATCAACCAAAA
TCTAAACAAAAACCAATTGATAATAGTAAAGCAAAATTGAATACACCAAAAGTTGTTGAT
ATGGCGGAGGATGCAAAATATGTTGATTCACATGTACATGTTGACCAACTTTTAATAAGA
TTAAATAAATCATTAACAGATTTCCCACAATTTAAACAA----------AATTAATTCAA
GTTTGTTGTGATCCAGTATCGATAGAATATGCAGATTTCTTAATTGAAAACAATGAGTGT
ATCTATGGTGCCTATGGCGTCCACCCTCACAATGCCAAGGAATATAATGACCAAATTGAG
CAAAAACTAATAGACAGGATGAAATCAAATCCAAAAGTTGTGGCATGGGGCGAGATGGGG
TTAGACTATTTTTACAATAAATCTTCAAATGACGAGCAAATCAATGCCTTCTCACGTCAA
TTGAAGCAGGCTGTCAAGTTGGGTAAACCATTGGTAATCCATAGTCGTGAGGCTGAGGAG
GACACATTGCGTCTCTTGAAGGAGTTGGTACCTGTTGATTATAAAATTCATATCCATTGT
TTCACGTCCAATAGTGATTTCGCAAAACAATTACTTGACCATTTCCCAAATCTTTGTATT
GGGTTCACTGGTTGCATCACTTTTAGAAACTCTCAATCAATCAGAGATAGCGTTGAAGCC
GTGCCAATCGAACGTATCCTTTTGGAAACTGATGGTCCATATATGACACCTGAACCATTC
CGTGGTCAAATTGCTCATTCTGGGCACATTCCAATGGTTGCAAATGCAATCTCTCAAATT
AAAAATATACCCCTAGATCAAGTTTTAAAACAATGTAGAGAAAATACTACTAAAATTTAG
GAATTTAAAAATAAAATAAAATCAAA