>VFB490 (VFB490Q) /CSM/VF/VFB4-D/VFB490Q.Seq.d/
AATTCTGACATTACAAGCATTGAAAAAAATGAACAAATNAATTTTATTATTAGTTTTAGT
AATTGTATCAATTATTGGTTCAATTGACTCAAAGAAAGTATTCAATGTTCGTACTGATTT
AAAAAATCAATTAAAATTTAGACATTTTGATGATAGTTTAGATTATGATGCAATCAATTA
TCAATGGTTCACTCAAAGTGTTGATCATTTTAATCCAGCAAACCCAACAACTTTCCAACA
AAGATATTTAATTAATGACCAATATTGGGATGGTACAGGTCCAGTTTTTATCATGATTAA
TGGTGAAGGTCCAATGGATATTAATACTGTAACCCAACTTCAATTTGTTGTTTGGGCTAA
ACAAGTTAGTGCACTTGTTGTTTCTCTTGAACATCGTTATTATGGTGCTAGTTTTGTAAC
TGAAGACCTTTCACTTGAAAATTTACAATGGTTAAATTCAGCACAAGCATTAGCAGATAA
TGCAGTTTTTAGAAATTTTGTAGCACAACAATATAATGTACCAAAAGAATCAAAATGGAT
TTCATTTGGTGGTAGTTATTCAGGAGCATTAACTTCATGGTTTAGAATXXXXXXXXXXCA
AATGTTTAACCTTTGTACACCACTTGGTAATCAAAATGATGTTGCCACTTTTATGCAATC
ACTTGCAGGTAATTTCATGGGTGTAGTTCAATATAACGATGAAGAACCAGGTCAAATCGA
TATCGATTACCTTTGCAATATCATGACTAATCAATCATCAGATCCATTGACAAACTATAT
CCAAATTTGGGATCAATATGCTGATGGTGAATGTGTTGATGTTTCATATGCTTCAATGAT
CGCTCAAAATCAAAATGTTACCAATGATGAAAATGCCATTGGTGGTAGAATGTGGTTCTA
TCAAACTTGTGTTGAATTTGGTTATTATCAATCAAGTGATGCTCCATCTGCAAATCAACC
ATTTGGTAATTTATTCCCATTCCAACCATATCAAATTCAACAATGTGCCGATAGTTTTGG
TATTCCAAATATGTATCCAAACGTTAATTGGACAATCACTGAATATGGTGGTATTAATCC
AGAACCATCCTCTGTTGATAATACCTTATATGTAAATGGTTCAAATGATGAATGGCACAA
TCTTGCAATCTTACCAGGTAATGCAAATGCTAAAAATACTTTATACATTATTGGTACAAG
TCATTGTGCTATANANATTCCAACTTCAGTATCACCTCCAACTCTGCTCAAGCACAACAA
ATATTTTNAATTCATCCAAGC

ILTLQALKKMNKXILLLVLVIVSIIGSIDSKKVFNVRTDLKNQLKFRHFDDSLDYDAINY
QWFTQSVDHFNPANPTTFQQRYLINDQYWDGTGPVFIMINGEGPMDINTVTQLQFVVWAK
QVSALVVSLEHRYYGASFVTEDLSLENLQWLNSAQALADNAVFRNFVAQQYNVPKESKWI
SFGGSYSGALTSWFR---

---QMFNLCTPLGNQNDVATFMQSLAGNFMGVVQYNDEEPGQIDIDYLCNIMTNQSSDPL
TNYIQIWDQYADGECVDVSYASMIAQNQNVTNDENAIGGRMWFYQTCVEFGYYQSSDAPS
ANQPFGNLFPFQPYQIQQCADSFGIPNMYPNVNWTITEYGGINPEPSSVDNTLYVNGSND
EWHNLAILPGNANAKNTLYIIGTSHCAIXIPTSVSPPTLLKHNKYFXFIQ

Frame A:
nsditsiekneqxnfiisfsncinywfn*lkesiqcsy*fkksiki*tf***frl*cnql
smvhskc*sf*sskpnnfptkifn**pilgwyrssfyhd*w*rsngy*ycnptsicclg*
ts*ctccfs*tsllwc*fcn*rpft*kftmvkfstsisr*csf*kfcstti*ctkrikmd
fiww*lfrsinfmv*n---

---QMFNLCTPLGNQNDVATFMQSLAGNFMGVVQYNDEEPGQIDIDYLCNIMTNQSSDPL
TNYIQIWDQYADGECVDVSYASMIAQNQNVTNDENAIGGRMWFYQTCVEFGYYQSSDAPS
ANQPFGNLFPFQPYQIQQCADSFGIPNMYPNVNWTITEYGGINPEPSSVDNTLYVNGSND
EWHNLAILPGNANAKNTLYIIGTSHCAIXIPTSVSPPTLLKHNKYFXFIQ

Frame B:
ILTLQALKKMNKXILLLVLVIVSIIGSIDSKKVFNVRTDLKNQLKFRHFDDSLDYDAINY
QWFTQSVDHFNPANPTTFQQRYLINDQYWDGTGPVFIMINGEGPMDINTVTQLQFVVWAK
QVSALVVSLEHRYYGASFVTEDLSLENLQWLNSAQALADNAVFRNFVAQQYNVPKESKWI
SFGGSYSGALTSWFR---

---kcltfvhhlvikmmlpllcnhlqviswv*fnitmknqvksisitfais*linhqih*
qtiskfginmlmvnvlmfhmlq*slkikmlpmmkmplvvecgsiklvlnlviinqvmlhl
qinhlviyshsnhikfnnvpivlvfqiciqtligqslnmvvliqnhplliipym*mvqmm
ngtilqsyqvmqmlkilytllvqvivlxxfqlqyhlqlcssttnixnssk

Frame C:
f*hykh*kk*tnxfyy*f**lyqllvqltqrkysmfvli*kin*nldilmiv*immqsii
ngslkvliiliqqtqqlsnkdi*lmtnigmvqvqfls*lmvkvqwilil*pnfnllfgln
klvhllfllnivimvlvl*lktfhlkiyng*iqhkh*qimqfleil*hnnimyqknqngf
hlvvviqeh*lhgle---

---nv*plyttw*sk*cchfyaitcr*fhgcssi*r*rtrsnryrlplqyhd*siirsid
klypnlgsic*w*mc*cficfndrskskcyq**kchww*nvvlsnlc*iwllsik*csic
kstiw*fipiptisnstmcr*fwyskyvskr*ldnh*iwwy*srtilc**ylickwfk**
maqscnltr*ckc*kyfihywykslcyxxsnfsitsnsaqaqqifxihps

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFB490 (VFB490Q) /CSM/VF/VFB4-D/VFB490Q.Seq.d/                       2415   0.0
VFJ809 (VFJ809Q) /CSM/VF/VFJ8-A/VFJ809Q.Seq.d/                       1336   0.0
SFL142 (SFL142Q) /CSM/SF/SFL1-B/SFL142Q.Seq.d/                       1336   0.0
SFC890 (SFC890Q) /CSM/SF/SFC8-D/SFC890Q.Seq.d/                       1336   0.0
CFA835 (CFA835Q) /CSM/CF/CFA8-B/CFA835Q.Seq.d/                       1336   0.0
VFB467 (VFB467Q) /CSM/VF/VFB4-C/VFB467Q.Seq.d/                       1326   0.0
VFO841 (VFO841Q) /CSM/VF/VFO8-B/VFO841Q.Seq.d/                       1322   0.0
VFO647 (VFO647Q) /CSM/VF/VFO6-B/VFO647Q.Seq.d/                       1322   0.0
VFK280 (VFK280Q) /CSM/VF/VFK2-D/VFK280Q.Seq.d/                       1322   0.0
VFJ739 (VFJ739Q) /CSM/VF/VFJ7-B/VFJ739Q.Seq.d/                       1322   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

BI920191|BI920191.1 EST540126 potato microtubers, in vitro-grown Solanum tuberosum cDNA clone cSTE24M6 5' end, mRNA sequence.    44  5e-05  2
AF297520|AF297520.1 Haemophilus ducreyi alanyl-tRNA synthase (alaS), carbon storage regulator (csrA), and UDP-glucose pyrophosphorylase (galU) genes, complete cds.    40  0.18  2
CB097614|CB097614.1 ku42h12.y1 Strongyloides ratti PA female naive pAMP1 v1 Strongyloides ratti cDNA 5' similar to SW:YM67_CAEEL P34528 HYPOTHETICAL 65.3 KD PROTEIN K12H4.7 IN CHROMOSOME III. [1] ;, mRNA sequence.    48  0.28  1
CB097763|CB097763.1 ku44g10.y1 Strongyloides ratti PA female naive pAMP1 v1 Strongyloides ratti cDNA 5' similar to SW:YM67_CAEEL P34528 HYPOTHETICAL 65.3 KD PROTEIN K12H4.7 IN CHROMOSOME III. [1] ;, mRNA sequence.    48  0.28  1
BI178421|BI178421.1 EST519366 cSTE Solanum tuberosum cDNA clone cSTE13N22 5' sequence, mRNA sequence.    40  0.33  2
AP004475|AP004475.1 Lotus japonicus genomic DNA, chromosome 1, clone:LjT01I13, TM0010.    44  0.65  3
AE004376|AE004376.1 Vibrio cholerae chromosome II, section 33 of 93 of the complete chromosome.    38  0.73  3
AC084578|AC084578.1 Caenorhabditis briggsae cosmid G39O07, complete sequence.    40  0.91  2
AP004173|AP004173.1 Mycoplasma penetrans genomic DNA, complete sequence, section 4/5.    36  0.98  10
BI317694|BI317694.1 saf19h09.y1 Gm-c1076 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1076-1506 5', mRNA sequence.    46  1.1  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AE014296_1236(AE014296|pid:none) Drosophila melanogaster chromos...   139   3e-31
AE014297_2752(AE014297|pid:none) Drosophila melanogaster chromos...   138   5e-31
BC133755_1(BC133755|pid:none) Xenopus laevis hypothetical LOC495...   135   2e-30
BC108759_1(BC108759|pid:none) Xenopus laevis hypothetical protei...   135   2e-30
EF147757_1(EF147757|pid:none) Populus trichocarpa clone WS0124_N...   135   3e-30
EF085613_1(EF085613|pid:none) Picea sitchensis clone WS0289_O21 ...   133   2e-29
FB848274_1(FB848274|pid:none) Sequence 67547 from Patent WO20080...   132   2e-29
BT080886_1(BT080886|pid:none) Caligus clemensi clone ccle-evs-50...   130   8e-29
BC161646_1(BC161646|pid:none) Xenopus tropicalis hypothetical pr...   129   3e-28
BT030459_1(BT030459|pid:none) Arabidopsis thaliana At4g36190 mRN...   128   5e-28

 psg: 0.77  gvh: 0.72  alm: 0.20  top: 0.40  tms: 0.07  mit: 0.29  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 20.0 %: cytoplasmic
	 20.0 %: endoplasmic reticulum
	 16.0 %: Golgi
	 16.0 %: nuclear
	 12.0 %: mitochondrial
	  4.0 %: extracellular, including cell wall
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system
	  4.0 %: peroxisomal

>> prediction for VFB490 is cyt

>VFB490F.Seq
AATTCTGACATTACAAGCATTGAAAAAAATGAACAAATNAATTTTATTATTAGTTTTAGT
AATTGTATCAATTATTGGTTCAATTGACTCAAAGAAAGTATTCAATGTTCGTACTGATTT
AAAAAATCAATTAAAATTTAGACATTTTGATGATAGTTTAGATTATGATGCAATCAATTA
TCAATGGTTCACTCAAAGTGTTGATCATTTTAATCCAGCAAACCCAACAACTTTCCAACA
AAGATATTTAATTAATGACCAATATTGGGATGGTACAGGTCCAGTTTTTATCATGATTAA
TGGTGAAGGTCCAATGGATATTAATACTGTAACCCAACTTCAATTTGTTGTTTGGGCTAA
ACAAGTTAGTGCACTTGTTGTTTCTCTTGAACATCGTTATTATGGTGCTAGTTTTGTAAC
TGAAGACCTTTCACTTGAAAATTTACAATGGTTAAATTCAGCACAAGCATTAGCAGATAA
TGCAGTTTTTAGAAATTTTGTAGCACAACAATATAATGTACCAAAAGAATCAAAATGGAT
TTCATTTGGTGGTAGTTATTCAGGAGCATTAACTTCATGGTTTAGAAT----------

>VFB490Z.Seq
----------CAAATGTTTAACCTTTGTACACCACTTGGTAATCAAAATGATGTTGCCAC
TTTTATGCAATCACTTGCAGGTAATTTCATGGGTGTAGTTCAATATAACGATGAAGAACC
AGGTCAAATCGATATCGATTACCTTTGCAATATCATGACTAATCAATCATCAGATCCATT
GACAAACTATATCCAAATTTGGGATCAATATGCTGATGGTGAATGTGTTGATGTTTCATA
TGCTTCAATGATCGCTCAAAATCAAAATGTTACCAATGATGAAAATGCCATTGGTGGTAG
AATGTGGTTCTATCAAACTTGTGTTGAATTTGGTTATTATCAATCAAGTGATGCTCCATC
TGCAAATCAACCATTTGGTAATTTATTCCCATTCCAACCATATCAAATTCAACAATGTGC
CGATAGTTTTGGTATTCCAAATATGTATCCAAACGTTAATTGGACAATCACTGAATATGG
TGGTATTAATCCAGAACCATCCTCTGTTGATAATACCTTATATGTAAATGGTTCAAATGA
TGAATGGCACAATCTTGCAATCTTACCAGGTAATGCAAATGCTAAAAATACTTTATACAT
TATTGGTACAAGTCATTGTGCTATANANATTCCAACTTCAGTATCACCTCCAACTCTGCT
CAAGCACAACAAATATTTTNAATTCATCCAAGC

>VFB490P.Seq
AATTCTGACATTACAAGCATTGAAAAAAATGAACAAATNAATTTTATTATTAGTTTTAGT
AATTGTATCAATTATTGGTTCAATTGACTCAAAGAAAGTATTCAATGTTCGTACTGATTT
AAAAAATCAATTAAAATTTAGACATTTTGATGATAGTTTAGATTATGATGCAATCAATTA
TCAATGGTTCACTCAAAGTGTTGATCATTTTAATCCAGCAAACCCAACAACTTTCCAACA
AAGATATTTAATTAATGACCAATATTGGGATGGTACAGGTCCAGTTTTTATCATGATTAA
TGGTGAAGGTCCAATGGATATTAATACTGTAACCCAACTTCAATTTGTTGTTTGGGCTAA
ACAAGTTAGTGCACTTGTTGTTTCTCTTGAACATCGTTATTATGGTGCTAGTTTTGTAAC
TGAAGACCTTTCACTTGAAAATTTACAATGGTTAAATTCAGCACAAGCATTAGCAGATAA
TGCAGTTTTTAGAAATTTTGTAGCACAACAATATAATGTACCAAAAGAATCAAAATGGAT
TTCATTTGGTGGTAGTTATTCAGGAGCATTAACTTCATGGTTTAGAAT----------CA
AATGTTTAACCTTTGTACACCACTTGGTAATCAAAATGATGTTGCCACTTTTATGCAATC
ACTTGCAGGTAATTTCATGGGTGTAGTTCAATATAACGATGAAGAACCAGGTCAAATCGA
TATCGATTACCTTTGCAATATCATGACTAATCAATCATCAGATCCATTGACAAACTATAT
CCAAATTTGGGATCAATATGCTGATGGTGAATGTGTTGATGTTTCATATGCTTCAATGAT
CGCTCAAAATCAAAATGTTACCAATGATGAAAATGCCATTGGTGGTAGAATGTGGTTCTA
TCAAACTTGTGTTGAATTTGGTTATTATCAATCAAGTGATGCTCCATCTGCAAATCAACC
ATTTGGTAATTTATTCCCATTCCAACCATATCAAATTCAACAATGTGCCGATAGTTTTGG
TATTCCAAATATGTATCCAAACGTTAATTGGACAATCACTGAATATGGTGGTATTAATCC
AGAACCATCCTCTGTTGATAATACCTTATATGTAAATGGTTCAAATGATGAATGGCACAA
TCTTGCAATCTTACCAGGTAATGCAAATGCTAAAAATACTTTATACATTATTGGTACAAG
TCATTGTGCTATANANATTCCAACTTCAGTATCACCTCCAACTCTGCTCAAGCACAACAA
ATATTTTNAATTCATCCAAGC