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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFB489
Library VF
(Link to library)
Clone ID VFB489
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16382-1
Original site URL
Representative seq. ID VFB489P
(Link to Original site)
Representative DNA sequence 
>VFB489 (VFB489Q) /CSM/VF/VFB4-D/VFB489Q.Seq.d/
ATAATTTAAAACTCTAAAATAAATAAAATATAATATAAAATGGATGGTGAAGATGTTCAA
GCTTTAGTAATTGATAACGGTTCTGGTATGTGTAAAGCCGGTTTTGCTGGTGATGATGCC
CCACGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGACACACTGGTGTTATGGTTGXX
XXXXXXXXGNAATTGTCAGAGATATTAAAGAGAAATTAGCCTANGTCGCTCTCGACTTTG
AAGCTGAAATGCAAACTGCTGCTTCATCATCAGCATTAGAAAAATCATACGAATTACCAG
ACGGTCAAGTTATTACCATCGGTAATGAACGTTTCCGTTGTCCAGAAGCCCTCTTCCAAC
CATCATTCTTAGGTATGGAATCAGCTGGTATCCACGAAACCACCTACAACTCCATCATGA
AATGTGATGTCGATATCCGTAAAGATTTATACGGTAATGTCGTCTTATCAGGTGGTACAA
CTATGTTCCCAGGTATTGCTGATCGTATGAACAAAGAATTAACTGCATTAGCACCATCAA
CCATGAAAATTAAAATTATTGCACCACCAGAACGTAAATACTCAGTTTGGATCGGTGGAT
CTATTTTAGCTTCACTCTCAACTTTCCAACAAATGTGGATTTCAAAAGAAGAATATGACG
AATCAGGTCCATCAATGTCCACAGAAAAT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
ii*nskinki*YKMDGEDVQALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHTGVMV-
--

---XIVRDIKEKLAXVALDFEAEMQTAASSSALEKSYELPDGQVITIGNERFRCPEALFQ
PSFLGMESAGIHETTYNSIMKCDVDIRKDLYGNVVLSGGTTMFPGIADRMNKELTALAPS
TMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKEEYDESGPSMSTEN
Translated Amino Acid sequence (All Frames) 
Frame A:
ii*nskinki*YKMDGEDVQALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHTGVMV-
--

---XIVRDIKEKLAXVALDFEAEMQTAASSSALEKSYELPDGQVITIGNERFRCPEALFQ
PSFLGMESAGIHETTYNSIMKCDVDIRKDLYGNVVLSGGTTMFPGIADRMNKELTALAPS
TMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKEEYDESGPSMSTEN

Frame B:
*fktlk*ikynikwmvkmfkl**litvlvcvkpvllvmmphvlfshqllvvqdtlvlwl-
--

---xlseilkrn*pxslstlklkcklllhhqh*knhtnyqtvkllpsvmnvsvvqkpssn
hhs*vwnqlvstkppttps*nvmsisvkiytvmssyqvvqlcsqvlliv*tkn*lh*hhq
p*klkllhhqnvntqfgsvdlf*lhsqlsnkcgfqkknmtnqvhqcpqk

Frame C:
nlkl*nk*nii*ngw*rcssfsn**rfwyv*srfcw**cptccfpincwsskthwcyg--
-

---ncqry*reislxrsrl*s*nanccfiisirkiiritrrssyyhr**tfplsrsplpt
iilrygiswyprnhlqlhhem*cryp*rfir*crlirwynyvpryc*syeqrincistin
hen*nycttrt*ilsldrwiyfsftlnfptnvdfkrri*rirsinvhrk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFB489 (VFB489Q) /CSM/VF/VFB4-D/VFB489Q.Seq.d/                       1215   0.0
VFN306 (VFN306Q) /CSM/VF/VFN3-A/VFN306Q.Seq.d/                        969   0.0
VFL794 (VFL794Q) /CSM/VF/VFL7-D/VFL794Q.Seq.d/                        963   0.0
CHA122 (CHA122Q) /CSM/CH/CHA1-A/CHA122Q.Seq.d/                        963   0.0
SLB173 (SLB173Q) /CSM/SL/SLB1-D/SLB173Q.Seq.d/                        961   0.0
AFN183 (AFN183Q) /CSM/AF/AFN1-D/AFN183Q.Seq.d/                        961   0.0
VFI430 (VFI430Q) /CSM/VF/VFI4-B/VFI430Q.Seq.d/                        938   0.0
VFH562 (VFH562Q) /CSM/VF/VFH5-C/VFH562Q.Seq.d/                        938   0.0
VFD511 (VFD511Q) /CSM/VF/VFD5-A/VFD511Q.Seq.d/                        938   0.0
VFC602 (VFC602Q) /CSM/VF/VFC6-A/VFC602Q.Seq.d/                        938   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.   957  0.0  3
M14146|M14146.1 D.discoideum actin 15 gene, complete cds.        783  0.0  3
AC115579|AC115579.2 Dictyostelium discoideum chromosome 2 map 4915084-5005461 strain AX4, complete sequence.   783  0.0  3
AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.   783  0.0  3
X03281|X03281.1 Dictyostelium discoideum gene for actin A8.      751  0.0  2
X03282|X03282.1 dictyostelium discoideum gene for actin A12.     743  0.0  2
AC116956|AC116956.2 Dictyostelium discoideum chromosome 2 map 1418423-1684967 strain AX4, complete sequence.   735  0.0  3
AC115577|AC115577.2 Dictyostelium discoideum chromosome 2 map 4657875-4914984 strain AX4, complete sequence.   696  0.0  8
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.   613  0.0  3
X03283|X03283.1 Dictyostelium discoideum gene for actin A3-S1.   547  0.0  2
dna update 2003. 9. 3
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54H30) RecName: Full=Major actin; AltName: Full=Actin-1; AltNa...   318   7e-86
X03282_1(X03282|pid:none) dictyostelium discoideum gene for acti...   318   7e-86
B23412(B23412)actin 12 - slime mold (Dictyostelium discoideum)        318   7e-86
ATDO(A93223;A92871;S67999;A03004)actin - slime mold (Dictyosteli...   314   1e-84
(P02576) RecName: Full=Actin, plasmodial isoform;  &ATFY(A29044;...   314   1e-84
(Q553U6) RecName: Full=Putative actin-22;  &AC116305_3(AC116305|...   314   1e-84
(P07829) RecName: Full=Actin-3; AltName: Full=Actin-3-sub 1;          311   9e-84
AF508263_1(AF508263|pid:none) Pyrocystis lunula actin mRNA, comp...   311   1e-83
AY615868_1(AY615868|pid:none) Amastigomonas marina actin gene, p...   310   2e-83
AY294153_1(AY294153|pid:none) Platyamoeba placida actin mRNA, pa...   309   6e-83
protein update 2009. 6.17
PSORT 

 psg: 0.75  gvh: 0.34  alm: 0.46  top: 0.53  tms: 0.00  mit: 0.15  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 40.0 %: nuclear
	  8.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: mitochondrial
	  4.0 %: vesicles of secretory system

>> prediction for VFB489 is cyt
5' end seq. ID VFB489F
5' end seq. 
>VFB489F.Seq
ATAATTTAAAACTCTAAAATAAATAAAATATAATATAAAATGGATGGTGAAGATGTTCAA
GCTTTAGTAATTGATAACGGTTCTGGTATGTGTAAAGCCGGTTTTGCTGGTGATGATGCC
CCACGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGACACACTGGTGTTATGGTTG--
--------
Length of 5' end seq. 178
3' end seq. ID VFB489Z
3' end seq. 
>VFB489Z.Seq
----------GNAATTGTCAGAGATATTAAAGAGAAATTAGCCTANGTCGCTCTCGACTT
TGAAGCTGAAATGCAAACTGCTGCTTCATCATCAGCATTAGAAAAATCATACGAATTACC
AGACGGTCAAGTTATTACCATCGGTAATGAACGTTTCCGTTGTCCAGAAGCCCTCTTCCA
ACCATCATTCTTAGGTATGGAATCAGCTGGTATCCACGAAACCACCTACAACTCCATCAT
GAAATGTGATGTCGATATCCGTAAAGATTTATACGGTAATGTCGTCTTATCAGGTGGTAC
AACTATGTTCCCAGGTATTGCTGATCGTATGAACAAAGAATTAACTGCATTAGCACCATC
AACCATGAAAATTAAAATTATTGCACCACCAGAACGTAAATACTCAGTTTGGATCGGTGG
ATCTATTTTAGCTTCACTCTCAACTTTCCAACAAATGTGGATTTCAAAAGAAGAATATGA
CGAATCAGGTCCATCAATGTCCACAGAAAAT
Length of 3' end seq. 501
Connected seq. ID VFB489P
Connected seq. 
>VFB489P.Seq
ATAATTTAAAACTCTAAAATAAATAAAATATAATATAAAATGGATGGTGAAGATGTTCAA
GCTTTAGTAATTGATAACGGTTCTGGTATGTGTAAAGCCGGTTTTGCTGGTGATGATGCC
CCACGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGACACACTGGTGTTATGGTTG--
--------GNAATTGTCAGAGATATTAAAGAGAAATTAGCCTANGTCGCTCTCGACTTTG
AAGCTGAAATGCAAACTGCTGCTTCATCATCAGCATTAGAAAAATCATACGAATTACCAG
ACGGTCAAGTTATTACCATCGGTAATGAACGTTTCCGTTGTCCAGAAGCCCTCTTCCAAC
CATCATTCTTAGGTATGGAATCAGCTGGTATCCACGAAACCACCTACAACTCCATCATGA
AATGTGATGTCGATATCCGTAAAGATTTATACGGTAATGTCGTCTTATCAGGTGGTACAA
CTATGTTCCCAGGTATTGCTGATCGTATGAACAAAGAATTAACTGCATTAGCACCATCAA
CCATGAAAATTAAAATTATTGCACCACCAGAACGTAAATACTCAGTTTGGATCGGTGGAT
CTATTTTAGCTTCACTCTCAACTTTCCAACAAATGTGGATTTCAAAAGAAGAATATGACG
AATCAGGTCCATCAATGTCCACAGAAAAT
Length of connected seq. 679
Full length Seq ID -
Full length Seq. -
Length of full length seq. -