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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFM177
Library AF
(Link to library)
Clone ID AFM177
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID AFM177P
(Link to Original site)
Representative DNA sequence 
>AFM177 (AFM177Q) /CSM/AF/AFM1-D/AFM177Q.Seq.d/
GGCAGTTGGACATTGCCATATTGATTTATGCTGGTTATGGAGTTTTGAAAAGACCAAAGA
GAAATGTGCAAGATCATTCTCCACACAAATCCTCTACATGGATTACTACCCACAATTTAA
ATTCACCCAATCTCAAGCACAAGCTTACCAATGGACCAAAGAGAACTATCCAGAACTTTA
TGAAAGAATCAAAGAGAAAGTAGTCACTGGCCAATTCATCCCCACCGGTGGTACATGGGT
CGAAATGGATGGTAATCTCCCATCTGGTGAATCATTCATCCGTCAGTTCCTATACGGCCA
AAGATTCTTTGAAAAGGAATTTGGTAAAAAATGCACAGAATTCTTTTTACCAGATACATT
TGGTTACTCTGCTCAATTGCCACAAGTTATTCGTCATATGGGTATTGAAAATTTTATCAC
TCAAAAATTGTCTTGGAATAATTTAAATAAATTCCCACATAGTACATTCATTTGGGAAGG
AATTGACGGATCAAGTGTGTTAACTCATTTCCCACCTGCTGATACTTATAATTCACAAGC
CGATGTAAAGXXXXXXXXXXCGATTTCGATACCAATATTAGAGCAAAGAATGCAAATTAT
GAAATTCAATTTGGTCATATCGAACGTCCAACTCATTATAACACAAGTTGGGATTTCGCA
AGATTTGAAGTTGTCGGACATAAATGGGCCGATCTTAGTGAATATGATTTTGGTATGGCA
TTATTGAACGATTGTAAATATGGTTACTCAACATTAGGTGGTCGTATTGGTTTATCATTA
CTAAGATCACCAAAATCACCCGATGATACTTGTGATATGGGTTCTCATAAATTCACCTAC
TCAATTTATCCACATCGTGGTTCACTTCAATCTGCCTCCGTCATTAAAGAAGGCTATTCA
TTAAATAATAACTTTTATATCAGTGAAACACCATTCTCTTTAGCTTCTACAACTCATATC
GATAAAACTTTTATCTCAACAAATAAAGAAGCCATCATTGTTGATACAATTAAAAAAGCT
GAAGATGGCACCTCTTTTGTTGTGCGTGTTTATGAATCTTTTGGTGGTGCCACTACTTTT
AACTTTACATCTTCAATCCTTCCAATTCCATTCAAATCAATCATTGAATGTAATGGTCTT
GAAGAAGTTAATCAATCTTCAAAATCTTATAAATTTAATGATACAATTAAAATTAATCCA
TTTGAAATCAAAACTTTTAGATTTATTTCAAATTAAAAATTAAAAATAAAATTAATAAAT
AAATATAAAAATAAATTT
sequence update 2009. 4. 3
Translated Amino Acid sequence 
AVGHCHIDLCWLWSFEKTKEKCARSFSTQILYMDYYPQFKFTQSQAQAYQWTKENYPELY
ERIKEKVVTGQFIPTGGTWVEMDGNLPSGESFIRQFLYGQRFFEKEFGKKCTEFFLPDTF
GYSAQLPQVIRHMGIENFITQKLSWNNLNKFPHSTFIWEGIDGSSVLTHFPPADTYNSQA
DVK---

---DFDTNIRAKNANYEIQFGHIERPTHYNTSWDFARFEVVGHKWADLSEYDFGMALLND
CKYGYSTLGGRIGLSLLRSPKSPDDTCDMGSHKFTYSIYPHRGSLQSASVIKEGYSLNNN
FYISETPFSLASTTHIDKTFISTNKEAIIVDTIKKAEDGTSFVVRVYESFGGATTFNFTS
SILPIPFKSIIECNGLEEVNQSSKSYKFNDTIKINPFEIKTFRFISN*klkiklinkykn
kf
Translated Amino Acid sequence (All Frames) 
Frame A:
gswtlpy*fmlvmef*kdqremckiilhtnplhgllpti*ihpisstslpmdqrelsrtl
*knqresshwpihphrwymgrngw*spiw*iihpsvpirpkil*kgiw*kmhrilftryi
wllcsiatsyssygy*kfyhskivle*fk*ipt*yihlgrn*rikcvnsfptc*yl*fts
rck---

---rfryqy*skeckl*nsiwsyrtsnsl*hklgfrki*scrt*mgrs**i*fwygiier
l*iwllnirwsywfiitkitkitr*yl*ygfs*ihllnlstswftsiclrh*rrlfik**
llyq*ntilfsfynsyr*nfylnk*rshhc*yn*ks*rwhlfccacl*ifwwchyf*lyi
fnpsnsiqinh*m*ws*rs*sifkil*i**yn*n*si*nqnf*iyfklkiknkink*i*k
*i

Frame B:
AVGHCHIDLCWLWSFEKTKEKCARSFSTQILYMDYYPQFKFTQSQAQAYQWTKENYPELY
ERIKEKVVTGQFIPTGGTWVEMDGNLPSGESFIRQFLYGQRFFEKEFGKKCTEFFLPDTF
GYSAQLPQVIRHMGIENFITQKLSWNNLNKFPHSTFIWEGIDGSSVLTHFPPADTYNSQA
DVK---

---DFDTNIRAKNANYEIQFGHIERPTHYNTSWDFARFEVVGHKWADLSEYDFGMALLND
CKYGYSTLGGRIGLSLLRSPKSPDDTCDMGSHKFTYSIYPHRGSLQSASVIKEGYSLNNN
FYISETPFSLASTTHIDKTFISTNKEAIIVDTIKKAEDGTSFVVRVYESFGGATTFNFTS
SILPIPFKSIIECNGLEEVNQSSKSYKFNDTIKINPFEIKTFRFISN*klkiklinkykn
kf

Frame C:
qldiailiyagygvlkrpkrnvqdhsphksstwitthnlnspnlkhkltngpkrtiqnfm
keskrk*slanssppvvhgskwmvishlvnhssvssytakdslkrnlvknaqnsfyqihl
vtllnchklfviwvlkilslknclgii*inshivhsfgkeltdqvc*lishlliliihkp
m*---

---isipileqrmqimkfnlvisnvqliitqvgisqdlklsdingpilvnmilvwhy*ti
vnmvtqh*vvvlvyhy*dhqnhpmilviwvlinsptqfihivvhfnlppslkkaih*iit
fisvkhhsl*llqlisikllsqqikkpslliqlkklkmaplllcvfmnllvvpllltlhl
qsfqfhsnqslnvmvlkklinlqnlinlmiqlklihlksklldlfqikn*k*n**iniki
n
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFM177 (AFM177Q) /CSM/AF/AFM1-D/AFM177Q.Seq.d/                       2288   0.0
SHF873 (SHF873Q) /CSM/SH/SHF8-D/SHF873Q.Seq.d/                       1237   0.0
SHF554 (SHF554Q) /CSM/SH/SHF5-C/SHF554Q.Seq.d/                       1237   0.0
SHA333 (SHA333Q) /CSM/SH/SHA3-B/SHA333Q.Seq.d/                       1237   0.0
CHQ611 (CHQ611Q) /CSM/CH/CHQ6-A/CHQ611Q.Seq.d/                       1237   0.0
AHR366 (AHR366Q) /CSM/AH/AHR3-C/AHR366Q.Seq.d/                       1237   0.0
AHN834 (AHN834Q) /CSM/AH/AHN8-B/AHN834Q.Seq.d/                       1237   0.0
AHL346 (AHL346Q) /CSM/AH/AHL3-B/AHL346Q.Seq.d/                       1237   0.0
CHE158 (CHE158Q) /CSM/CH/CHE1-C/CHE158Q.Seq.d/                       1233   0.0
AHK633 (AHK633Q) /CSM/AH/AHK6-B/AHK633Q.Seq.d/                       1231   0.0
own update 2009. 4. 3
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ406580) Dictyostelium discoideum cDNA clone:dds35b20, 3' ...  1237   0.0    1
(BJ406544) Dictyostelium discoideum cDNA clone:dds35k13, 3' ...  1237   0.0    1
(BJ403159) Dictyostelium discoideum cDNA clone:dds24b09, 3' ...  1237   0.0    1
(BJ385463) Dictyostelium discoideum cDNA clone:ddc55e04, 3' ...  1237   0.0    1
(BJ358029) Dictyostelium discoideum cDNA clone:dda65d17, 3' ...  1237   0.0    1
(BJ355810) Dictyostelium discoideum cDNA clone:dda58d10, 3' ...  1237   0.0    1
(BJ354373) Dictyostelium discoideum cDNA clone:dda53l11, 3' ...  1237   0.0    1
(BJ347016) Dictyostelium discoideum cDNA clone:dda25i19, 3' ...  1237   0.0    1
(BJ376906) Dictyostelium discoideum cDNA clone:ddc30c15, 3' ...  1233   0.0    1
(BJ354013) Dictyostelium discoideum cDNA clone:dda52a10, 3' ...  1231   0.0    1
dna update 2009. 4. 9
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54K67) RecName: Full=Alpha-mannosidase G;          EC=3.2.1.24;     432   e-120
BC070781_1(BC070781|pid:none) Xenopus laevis hypothetical protei...   265   3e-69
BC127332_1(BC127332|pid:none) Xenopus tropicalis hypothetical pr...   261   4e-68
BC105160_1(BC105160|pid:none) Bos taurus mannosidase, alpha, cla...   259   1e-67
CU633901_161(CU633901|pid:none) Podospora anserina genomic DNA c...   259   2e-67
AF044414_1(AF044414|pid:none) Homo sapiens alpha mannosidase 6A8...   257   7e-67
(Q9NTJ4) RecName: Full=Alpha-mannosidase 2C1;          EC=3.2.1....   257   7e-67
AJ851692_1(AJ851692|pid:none) Gallus gallus mRNA for hypothetica...   256   1e-66
AK301170_1(AK301170|pid:none) Homo sapiens cDNA FLJ60004 complet...   255   2e-66
AK049370_1(AK049370|pid:none) Mus musculus ES cells cDNA, RIKEN ...   254   3e-66
protein update 2009. 4. 5
PSORT 

 psg: 0.00  gvh: 0.00  alm: 0.00  top: 0.00  tms: 0.00  mit: 0.00  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	100.0 %: 

>> prediction for AFM177 is

5' end seq. ID AFM177F
5' end seq. 
>AFM177F.Seq
GGCAGTTGGACATTGCCATATTGATTTATGCTGGTTATGGAGTTTTGAAAAGACCAAAGA
GAAATGTGCAAGATCATTCTCCACACAAATCCTCTACATGGATTACTACCCACAATTTAA
ATTCACCCAATCTCAAGCACAAGCTTACCAATGGACCAAAGAGAACTATCCAGAACTTTA
TGAAAGAATCAAAGAGAAAGTAGTCACTGGCCAATTCATCCCCACCGGTGGTACATGGGT
CGAAATGGATGGTAATCTCCCATCTGGTGAATCATTCATCCGTCAGTTCCTATACGGCCA
AAGATTCTTTGAAAAGGAATTTGGTAAAAAATGCACAGAATTCTTTTTACCAGATACATT
TGGTTACTCTGCTCAATTGCCACAAGTTATTCGTCATATGGGTATTGAAAATTTTATCAC
TCAAAAATTGTCTTGGAATAATTTAAATAAATTCCCACATAGTACATTCATTTGGGAAGG
AATTGACGGATCAAGTGTGTTAACTCATTTCCCACCTGCTGATACTTATAATTCACAAGC
CGATGTAAAGXXXXXXXXXX
Length of 5' end seq. 560
3' end seq. ID AFM177Z
3' end seq. 
>AFM177Z.Seq
XXXXXXXXXXCGATTTCGATACCAATATTAGAGCAAAGAATGCAAATTATGAAATTCAAT
TTGGTCATATCGAACGTCCAACTCATTATAACACAAGTTGGGATTTCGCAAGATTTGAAG
TTGTCGGACATAAATGGGCCGATCTTAGTGAATATGATTTTGGTATGGCATTATTGAACG
ATTGTAAATATGGTTACTCAACATTAGGTGGTCGTATTGGTTTATCATTACTAAGATCAC
CAAAATCACCCGATGATACTTGTGATATGGGTTCTCATAAATTCACCTACTCAATTTATC
CACATCGTGGTTCACTTCAATCTGCCTCCGTCATTAAAGAAGGCTATTCATTAAATAATA
ACTTTTATATCAGTGAAACACCATTCTCTTTAGCTTCTACAACTCATATCGATAAAACTT
TTATCTCAACAAATAAAGAAGCCATCATTGTTGATACAATTAAAAAAGCTGAAGATGGCA
CCTCTTTTGTTGTGCGTGTTTATGAATCTTTTGGTGGTGCCACTACTTTTAACTTTACAT
CTTCAATCCTTCCAATTCCATTCAAATCAATCATTGAATGTAATGGTCTTGAAGAAGTTA
ATCAATCTTCAAAATCTTATAAATTTAATGATACAATTAAAATTAATCCATTTGAAATCA
AAACTTTTAGATTTATTTCAAATTAAAAATTAAAAATAAAATTAATAAATAAATATAAAA
ATAAATTT
Length of 3' end seq. 728
Connected seq. ID AFM177P
Connected seq. 
>AFM177P.Seq
GGCAGTTGGACATTGCCATATTGATTTATGCTGGTTATGGAGTTTTGAAAAGACCAAAGA
GAAATGTGCAAGATCATTCTCCACACAAATCCTCTACATGGATTACTACCCACAATTTAA
ATTCACCCAATCTCAAGCACAAGCTTACCAATGGACCAAAGAGAACTATCCAGAACTTTA
TGAAAGAATCAAAGAGAAAGTAGTCACTGGCCAATTCATCCCCACCGGTGGTACATGGGT
CGAAATGGATGGTAATCTCCCATCTGGTGAATCATTCATCCGTCAGTTCCTATACGGCCA
AAGATTCTTTGAAAAGGAATTTGGTAAAAAATGCACAGAATTCTTTTTACCAGATACATT
TGGTTACTCTGCTCAATTGCCACAAGTTATTCGTCATATGGGTATTGAAAATTTTATCAC
TCAAAAATTGTCTTGGAATAATTTAAATAAATTCCCACATAGTACATTCATTTGGGAAGG
AATTGACGGATCAAGTGTGTTAACTCATTTCCCACCTGCTGATACTTATAATTCACAAGC
CGATGTAAAG----------CGATTTCGATACCAATATTAGAGCAAAGAATGCAAATTAT
GAAATTCAATTTGGTCATATCGAACGTCCAACTCATTATAACACAAGTTGGGATTTCGCA
AGATTTGAAGTTGTCGGACATAAATGGGCCGATCTTAGTGAATATGATTTTGGTATGGCA
TTATTGAACGATTGTAAATATGGTTACTCAACATTAGGTGGTCGTATTGGTTTATCATTA
CTAAGATCACCAAAATCACCCGATGATACTTGTGATATGGGTTCTCATAAATTCACCTAC
TCAATTTATCCACATCGTGGTTCACTTCAATCTGCCTCCGTCATTAAAGAAGGCTATTCA
TTAAATAATAACTTTTATATCAGTGAAACACCATTCTCTTTAGCTTCTACAACTCATATC
GATAAAACTTTTATCTCAACAAATAAAGAAGCCATCATTGTTGATACAATTAAAAAAGCT
GAAGATGGCACCTCTTTTGTTGTGCGTGTTTATGAATCTTTTGGTGGTGCCACTACTTTT
AACTTTACATCTTCAATCCTTCCAATTCCATTCAAATCAATCATTGAATGTAATGGTCTT
GAAGAAGTTAATCAATCTTCAAAATCTTATAAATTTAATGATACAATTAAAATTAATCCA
TTTGAAATCAAAACTTTTAGATTTATTTCAAATTAAAAATTAAAAATAAAATTAATAAAT
AAATATAAAAATAAATTT
Length of connected seq. 1268
Full length Seq ID -
Full length Seq. -
Length of full length seq. -