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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFB457
Library VF
(Link to library)
Clone ID VFB457
Atlas ID -
NBRP ID
dictyBase ID
Link to Contig Contig-U03440-1
Original site URL
Representative seq. ID VFB457E
(Link to Original site)
Representative DNA sequence 
>VFB457 (VFB457Q) /CSM/VF/VFB4-C/VFB457Q.Seq.d/
ATTTTGAATAAAATTTAAATTTCTTACAATTGGAAAAGCACACACACACACCCACACTTA
CATATAGAGAGTAACAATGAGCAAAGAGATTTTTATTGGCATTGATGGTGGTGGTACAAA
AACATCAACGGTTGCAGTTGATTCAAATGGTCAAGAATTAGCAAGACATACATCACCATG
TAGTAACTATCATAGTGTTGGTGAAGATTTAGCAAAAGCAGCAATTAACGAAGGTATAAA
ATATGTAATTAGAAAAGTTAAGGAGACAATCACAGATGATGATAATAAAGAAGTTACAGT
TGGATCAATTTGTTTAGGTATGAGTGGTGTAGATAGAGAGAAAGATAAACTATTAGTTAA
ATCATGGGTAACTGAATTATTGGGAGAATCAATCAACTACAGCATTCACAACGATGCAAT
CGTAGCTTTATCAAGTGGAACTCAAGGTAAACTATTTGGAGTTGTCATTATCTGTGGCAC
AGGTTGCATTTCATTAGGATTCAATAGGGAGGGTGTCTCAGGCAGATCAGGTGGTTGGGG
ACCATTATTAGGCGACTATGGCAGTGGCTACCAAATTGGTTACGATATCCTCAGACATGT
TTTAAAAGCAAAAGATCAAGTTGGTCCAAAAACTTCACTAACTCAAGTTTTATTAGAAAA
ATTACAACTCACCAAAGAAGAAGATTTAATCTCATGGGCTTATGATCCAAAAACTCAAAG
TTGGCAAAAGTTTGCTCAATTATCACCTTTAGCTTTTGAGCAAGCCCAATTAGGTGATGA
AATTTCAAATTTAATTTTAGTTGATGCTGCAAATGCACTTTATGATTTAATTAATAGTGT
TATTAAAAAGTTGGGATTGGATAAAGAAGAGAAATTCCCATTGGTTTATACTGGAGGAAA
TATTGAGAGAAAAGGTATTCTTTCTGATCTTTTATCCAAGAAAATTATGGAAAATTATCC
AAATGCTAAATTTTAAATACTACTTGTGATCCTTCAATGGGTGCTGCTTTATGGCATTAA
CTCTAAGAAATAACTATTTTATCTTTTTTTTTCCAA
sequence update 2001. 6. 9
Translated Amino Acid sequence 
f*ikfkfltigkahththtyi*RVTMSKEIFIGIDGGGTKTSTVAVDSNGQELARHTSPC
SNYHSVGEDLAKAAINEGIKYVIRKVKETITDDDNKEVTVGSICLGMSGVDREKDKLLVK
SWVTELLGESINYSIHNDAIVALSSGTQGKLFGVVIICGTGCISLGFNREGVSGRSGGWG
PLLGDYGSGYQIGYDILRHVLKAKDQVGPKTSLTQVLLEKLQLTKEEDLISWAYDPKTQS
WQKFAQLSPLAFEQAQLGDEISNLILVDAANALYDLINSVIKKLGLDKEEKFPLVYTGGN
IERKGILSDLLSKKIMENYPNAKF*illvilqwvllyginskk*lfylffs
Translated Amino Acid sequence (All Frames) 
Frame A:
ilnki*isynwksththphlhiesnneqrdfywh*wwwykningcs*fkwsrisktyitm
**ls*cw*rfskssn*rrykicn*ks*gdnhr****rsyswinlfryewcr*rer*tis*
imgn*iigrinqlqhsqrcnrsfikwnsr*tiwschylwhrlhfiriq*ggclrqirwlg
tiirrlwqwlpnwlrypqtcfkskrsswsknftnssfirkitthqrrrfnlmgl*sknsk
lakvcsiitfsf*aspir**nfkfnfs*cckctl*fn**cy*kvgig*rreipiglywrk
y*ekrysf*sfiqenygklskc*ilnttcdpsmgaalwh*l*eitilsfffq


Frame B:
f*ikfkfltigkahththtyi*RVTMSKEIFIGIDGGGTKTSTVAVDSNGQELARHTSPC
SNYHSVGEDLAKAAINEGIKYVIRKVKETITDDDNKEVTVGSICLGMSGVDREKDKLLVK
SWVTELLGESINYSIHNDAIVALSSGTQGKLFGVVIICGTGCISLGFNREGVSGRSGGWG
PLLGDYGSGYQIGYDILRHVLKAKDQVGPKTSLTQVLLEKLQLTKEEDLISWAYDPKTQS
WQKFAQLSPLAFEQAQLGDEISNLILVDAANALYDLINSVIKKLGLDKEEKFPLVYTGGN
IERKGILSDLLSKKIMENYPNAKF*illvilqwvllyginskk*lfylffs


Frame C:
fe*nlnflqlekhthtptltyre*q*akrfllalmvvvqkhqrlqliqmvkn*qdihhhv
vtiivlvki*qkqqltkv*nm*leklrrqsqmmiikklqldqfv*v*vv*ierkiny*ln
hg*lnywenqsttafttmqs*lyqvelkvnylelslsvaqvafh*dsigrvsqadqvvgd
hy*atmavatklvtissdmf*kqkiklvqklh*lkfy*knynspkkki*shglmiqklkv
gksllnyhl*llskpn*vmkfqi*f*lmlqmhfmi*livllkswdwikkrnshwfileei
lrekvfflifyprklwkiiqmlnfkyyl*sfngccfmaltlrnnyfifffp
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFB457 (VFB457Q) /CSM/VF/VFB4-C/VFB457Q.Seq.d/                       1955   0.0
VFC880 (VFC880Q) /CSM/VF/VFC8-D/VFC880Q.Seq.d/                       1893   0.0
SFC471 (SFC471Q) /CSM/SF/SFC4-C/SFC471Q.Seq.d/                       1255   0.0
CFI247 (CFI247Q) /CSM/CF/CFI2-B/CFI247Q.Seq.d/                         42   0.017
SFL331 (SFL331Q) /CSM/SF/SFL3-B/SFL331Q.Seq.d/                         40   0.067
AFK513 (AFK513Q) /CSM/AF/AFK5-A/AFK513Q.Seq.d/                         40   0.067
AFI613 (AFI613Q) /CSM/AF/AFI6-A/AFI613Q.Seq.d/                         40   0.067
AFI249 (AFI249Q) /CSM/AF/AFI2-C/AFI249Q.Seq.d/                         40   0.067
VFE376 (VFE376Q) /CSM/VF/VFE3-D/VFE376Q.Seq.d/                         38   0.27
VHN533 (VHN533Q) /CSM/VH/VHN5-B/VHN533Q.Seq.d/                         36   1.1
own update 2004. 6. 1
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AL844509|AL844509.1 Plasmodium falciparum chromosome 13.          36  0.058  11
BZ984693|BZ984693.1 PUGGI79TD ZM_0.6_1.0_KB Zea mays genomic clone ZMMBTa378M13, DNA sequence.    38  0.21  3
AC108745|AC108745.7 Homo sapiens 3 BAC RP11-232M24 (Roswell Park Cancer Institute Human BAC Library) complete sequence.    38  0.23  4
AC102851|AC102851.2 Mus musculus clone RP24-444B2, WORKING DRAFT SEQUENCE, 15 unordered pieces.    40  0.25  4
AC068448|AC068448.10 Homo sapiens chromosome 15, clone RP11-624A21, complete sequence.    34  0.27  7
AB044658|AB044658.1 Ornithoptera victoriae mitochondrial gene for NADH dehydrogenase subunit 5, partial cds, country:Papua New Guinea,Gela.    38  0.43  2
AB027598|AB027598.1 Ornithoptera victoriae mitochondrial mRNA for NADH dehydrogenase subunit 5, partial cds.    38  0.43  2
AC114942|AC114942.2 Homo sapiens chromosome 5 clone CTD-2327J4, complete sequence.    34  0.51  7
AC077691|AC077691.1 Mus musculus chromosome 12 clone RP23-208A10 strain C57BL6/J, WORKING DRAFT SEQUENCE, 17 unordered pieces.    38  0.57  5
AE017037|AE017037.1 Bacillus anthracis str. Ames section 14 of 18 of the complete genome.    32  0.75  8
dna update 2003. 8.30
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54PM7) RecName: Full=N-acetyl-D-glucosamine kinase;          S...   597   e-169
AK227991_1(AK227991|pid:none) Arabidopsis thaliana mRNA for hypo...   201   5e-50
AC009917_16(AC009917|pid:none) Arabidopsis thaliana chromosome 1...   196   1e-48
EU964915_1(EU964915|pid:none) Zea mays clone 282525 ATPase mRNA,...   188   2e-46
AK101816_1(AK101816|pid:none) Oryza sativa Japonica Group cDNA c...   183   8e-45
AK319058_1(AK319058|pid:none) Arabidopsis thaliana AT1G30540 mRN...   182   1e-44
EU973919_1(EU973919|pid:none) Zea mays clone 424247 ATPase mRNA,...   182   2e-44
AP006716_193(AP006716|pid:none) Staphylococcus haemolyticus JCSC...   147   5e-34
AE001437_178(AE001437|pid:none) Clostridium acetobutylicum ATCC ...   143   1e-32
CP000724_4029(CP000724|pid:none) Alkaliphilus metalliredigens QY...   137   8e-31
protein update 2009. 7.10
PSORT 

 psg: 0.83  gvh: 0.35  alm: 0.26  top: 0.57  tms: 0.07  mit: 0.27  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.60  pox: 0.30  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.09  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 1.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: cytoplasmic
	 28.0 %: nuclear
	 20.0 %: mitochondrial
	  8.0 %: plasma membrane
	  8.0 %: endoplasmic reticulum
	  4.0 %: vesicles of secretory system
	  4.0 %: peroxisomal

>> prediction for VFB457 is cyt
5' end seq. ID VFB457F
5' end seq. 
>VFB457F.Seq
ATTTTGAATAAAATTTAAATTTCTTACAATTGGAAAAGCACACACACACACCCACACTTA
CATATAGAGAGTAACAATGAGCAAAGAGATTTTTATTGGCATTGATGGTGGTGGTACAAA
AACATCAACGGTTGCAGTTGATTCAAATGGTCAAGAATTAGCAAGACATACATCACCATG
TAGTAACTATCATAGTGTTGGTGAAGATTTAGCAAAAGCAGCAATTAACGAAGGTATAAA
ATATGTAATTAGAAAAGTTAAGGAGACAATCACAGATGATGATAATAAAGAAGTTACAGT
TGGATCAATTTGTTTAGGTATGAGTGGTGTAGATAGAGAGAAAGATAAACTATTAGTTAA
ATCATGGGTAACTGAATTATTGGGAGAATCAATCAACTACAGCATTCACAACGATGCAAT
CGTAGCTTTATCAAGTGGAACTCAAGGTAAACTATTTGGAGTTGTCATTATCTGTGGCAC
AGGTTGCATTTCATTAGGATTCAATAGGGAGGGTGTCTCAGGCAGATCAGGTGGTTGGGG
ACC----------
Length of 5' end seq. 543
3' end seq. ID VFB457Z
3' end seq. 
>VFB457Z.Seq
----------TTAAATCATGGGTAACTGAATTATTGGGAGAATCAATCAACTACAGCATT
CACAACGATGCAATCGTAGCTTTATCAAGTGGAACTCAAGGTAAACTATTTGGAGTTGTC
ATTATCTGTGGCACAGGTTGCATTTCATTAGGATTCAATAGGGAGGGTGTCTCAGGCAGA
TCAGGTGGTTGGGGACCATTATTAGGCGACTATGGCAGTGGCTACCAAATTGGTTACGAT
ATCCTCAGACATGTTTTAAAAGCAAAAGATCAAGTTGGTCCAAAAACTTCACTAACTCAA
GTTTTATTAGAAAAATTACAACTCACCAAAGAAGAAGATTTAATCTCATGGGCTTATGAT
CCAAAAACTCAAAGTTGGCAAAAGTTTGCTCAATTATCACCTTTAGCTTTTGAGCAAGCC
CAATTAGGTGATGAAATTTCAAATTTAATTTTAGTTGATGCTGCAAATGCACTTTATGAT
TTAATTAATAGTGTTATTAAAAAGTTGGGATTGGATAAAGAAGAGAAATTCCCATTGGTT
TATACTGGAGGAAATATTGAGAGAAAAGGTATTCTTTCTGATCTTTTATCCAAGAAAATT
ATGGAAAATTATCCAAATGCTAAATTTTAAATACTACTTGTGATCCTTCAATGGGTGCTG
CTTTATGGCATTAACTCTAAGAAATAACTATTTTATCTTTTTTTTTCCAA
Length of 3' end seq. 700
Connected seq. ID VFB457P
Connected seq. 
>VFB457P.Seq
ATTTTGAATAAAATTTAAATTTCTTACAATTGGAAAAGCACACACACACACCCACACTTA
CATATAGAGAGTAACAATGAGCAAAGAGATTTTTATTGGCATTGATGGTGGTGGTACAAA
AACATCAACGGTTGCAGTTGATTCAAATGGTCAAGAATTAGCAAGACATACATCACCATG
TAGTAACTATCATAGTGTTGGTGAAGATTTAGCAAAAGCAGCAATTAACGAAGGTATAAA
ATATGTAATTAGAAAAGTTAAGGAGACAATCACAGATGATGATAATAAAGAAGTTACAGT
TGGATCAATTTGTTTAGGTATGAGTGGTGTAGATAGAGAGAAAGATAAACTATTAGTTAA
ATCATGGGTAACTGAATTATTGGGAGAATCAATCAACTACAGCATTCACAACGATGCAAT
CGTAGCTTTATCAAGTGGAACTCAAGGTAAACTATTTGGAGTTGTCATTATCTGTGGCAC
AGGTTGCATTTCATTAGGATTCAATAGGGAGGGTGTCTCAGGCAGATCAGGTGGTTGGGG
ACC----------TTAAATCATGGGTAACTGAATTATTGGGAGAATCAATCAACTACAGC
ATTCACAACGATGCAATCGTAGCTTTATCAAGTGGAACTCAAGGTAAACTATTTGGAGTT
GTCATTATCTGTGGCACAGGTTGCATTTCATTAGGATTCAATAGGGAGGGTGTCTCAGGC
AGATCAGGTGGTTGGGGACCATTATTAGGCGACTATGGCAGTGGCTACCAAATTGGTTAC
GATATCCTCAGACATGTTTTAAAAGCAAAAGATCAAGTTGGTCCAAAAACTTCACTAACT
CAAGTTTTATTAGAAAAATTACAACTCACCAAAGAAGAAGATTTAATCTCATGGGCTTAT
GATCCAAAAACTCAAAGTTGGCAAAAGTTTGCTCAATTATCACCTTTAGCTTTTGAGCAA
GCCCAATTAGGTGATGAAATTTCAAATTTAATTTTAGTTGATGCTGCAAATGCACTTTAT
GATTTAATTAATAGTGTTATTAAAAAGTTGGGATTGGATAAAGAAGAGAAATTCCCATTG
GTTTATACTGGAGGAAATATTGAGAGAAAAGGTATTCTTTCTGATCTTTTATCCAAGAAA
ATTATGGAAAATTATCCAAATGCTAAATTTTAAATACTACTTGTGATCCTTCAATGGGTG
CTGCTTTATGGCATTAACTCTAAGAAATAACTATTTTATCTTTTTTTTTCCAA
Length of connected seq. 1243
Full length Seq ID VFB457E
Full length Seq. 
>VFB457E.Seq
ATTTTGAATAAAATTTAAATTTCTTACAATTGGAAAAGCACACACACACACCCACACTTA
CATATAGAGAGTAACAATGAGCAAAGAGATTTTTATTGGCATTGATGGTGGTGGTACAAA
AACATCAACGGTTGCAGTTGATTCAAATGGTCAAGAATTAGCAAGACATACATCACCATG
TAGTAACTATCATAGTGTTGGTGAAGATTTAGCAAAAGCAGCAATTAACGAAGGTATAAA
ATATGTAATTAGAAAAGTTAAGGAGACAATCACAGATGATGATAATAAAGAAGTTACAGT
TGGATCAATTTGTTTAGGTATGAGTGGTGTAGATAGAGAGAAAGATAAACTATTAGTTAA
ATCATGGGTAACTGAATTATTGGGAGAATCAATCAACTACAGCATTCACAACGATGCAAT
CGTAGCTTTATCAAGTGGAACTCAAGGTAAACTATTTGGAGTTGTCATTATCTGTGGCAC
AGGTTGCATTTCATTAGGATTCAATAGGGAGGGTGTCTCAGGCAGATCAGGTGGTTGGGG
ACCATTATTAGGCGACTATGGCAGTGGCTACCAAATTGGTTACGATATCCTCAGACATGT
TTTAAAAGCAAAAGATCAAGTTGGTCCAAAAACTTCACTAACTCAAGTTTTATTAGAAAA
ATTACAACTCACCAAAGAAGAAGATTTAATCTCATGGGCTTATGATCCAAAAACTCAAAG
TTGGCAAAAGTTTGCTCAATTATCACCTTTAGCTTTTGAGCAAGCCCAATTAGGTGATGA
AATTTCAAATTTAATTTTAGTTGATGCTGCAAATGCACTTTATGATTTAATTAATAGTGT
TATTAAAAAGTTGGGATTGGATAAAGAAGAGAAATTCCCATTGGTTTATACTGGAGGAAA
TATTGAGAGAAAAGGTATTCTTTCTGATCTTTTATCCAAGAAAATTATGGAAAATTATCC
AAATGCTAAATTTTAAATACTACTTGTGATCCTTCAATGGGTGCTGCTTTATGGCATTAA
CTCTAAGAAATAACTATTTTATCTTTTTTTTTCCAA
Length of full length seq. 1056