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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFM174
Library AF
(Link to library)
Clone ID AFM174
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID AFM174P
(Link to Original site)
Representative DNA sequence 
>AFM174 (AFM174Q) /CSM/AF/AFM1-D/AFM174Q.Seq.d/
ATTCAATTTTGTAATTATAAAAAAAAGGAATCAAATAATTATATATATATATAAAAATAT
AAAAAAAATAAAATGAGAATTTTATATTTAGCTTCTCTTTTATTTTTAATTACATTATAT
CTATCACCAACTTTTGGTTGGGGTGGTGGCAGAGATTGTGAAAGTCATCGTTCAGAATAT
ACATGTAAATCAGATAGAAGTTGTGCATATTTACCATTTGTATCATGTTGTGGTAAGAAA
GAATTTTTCTGTGTTAATCGTGATCATAGAAATTGTTGTGATGATTTATCATGTGCTAAA
AACACGAGAACTGGTGAAATCTTTGAGATTTGGTCAAGTTGTAAACCACATAGAGATTTT
GTACCATATCATTCACCAAATACAACCACATGTGAATCATTAGGTTGTGAAGCAAGAGGT
ATGGAATGTGAATGGGTTGAATCATCACCATGTTATGGAACTTCTTGTTGCCCAAGAATT
CCAAGATGTGTTGGTCATCATGGTGGCGGTCATAAATGTGACAGAATGAGATGTXXXXXX
XXXXTNNTNAATGTNCANNTGATTTNTCTTGTAAAATGATTAGAGGTAATNCAACTTGTA
TTAAAGCAAGACCAGCGGTACCACCACCACATTGCTCAACTTGTGCAGAGCTATCATCAG
CATGTAATCACGTTGGAATGATTTGTATTCAAGTACCAAGCAATTGTACCAATACTAGAT
TCCCATGTTGCCCATCTCATCCAATTTGTATTCATCCATCAACTACTGCTGCTTCAACCA
TTGCAACAACTGCATCAACTGTCGCAACTACAACCTCTGCAACTACTGCAGGTACAACAA
CTGGTGGAACTACAACTGGTGGTTCAACTTCTGATAGTAGTGCTGCTTCCTCAGCCGATA
GTAGCGCTGCCTCCTCATCACCATCAAGCAGTGCTGCTTCAAGTGCCGCTTCAAGTGAAC
CATCAAGTAGCGCCGCTTCAAGTAGTGCTCCATCCTCTGCTTCAAGTAGCGCTCCATCCT
CTGCCTCAAGTAGTGCTCCATCATCATCAGCTTCAAGTTCAGCTGCATCATCAGCTGCAA
GCTCAGAAAGTCNCAGAAAG
sequence update 2009. 4. 3
Translated Amino Acid sequence 
iqfcnykkkesnnyiyi*KYKKNKMRILYLASLLFLITLYLSPTFGWGGGRDCESHRSEY
TCKSDRSCAYLPFVSCCGKKEFFCVNRDHRNCCDDLSCAKNTRTGEIFEIWSSCKPHRDF
VPYHSPNTTTCESLGCEARGMECEWVESSPCYGTSCCPRIPRCVGHHGGGHKCDRMRC--
-

---XXCXXDXSCKMIRGNXTCIKARPAVPPPHCSTCAELSSACNHVGMICIQVPSNCTNT
RFPCCPSHPICIHPSTTAASTIATTASTVATTTSATTAGTTTGGTTTGGSTSDSSAASSA
DSSAASSSPSSSAASSAASSEPSSSAASSSAPSSASSSAPSSASSSAPSSSASSSAASSA
ASSESXRK
Translated Amino Acid sequence (All Frames) 
Frame A:
iqfcnykkkesnnyiyi*KYKKNKMRILYLASLLFLITLYLSPTFGWGGGRDCESHRSEY
TCKSDRSCAYLPFVSCCGKKEFFCVNRDHRNCCDDLSCAKNTRTGEIFEIWSSCKPHRDF
VPYHSPNTTTCESLGCEARGMECEWVESSPCYGTSCCPRIPRCVGHHGGGHKCDRMRC--
-

---xxmxx*fxl*nd*r*xnly*sktsgttttllnlcraiism*srwndlysstkqlyqy
*ipmlpissnlyssinyccfnhcnncincrnynlcnycrynnwwnynwwfnf***ccfls
r**rcllitikqccfkcrfk*tik*rrfk*csilcfk*rsilclk*csiiisfkfsciis
cklrksqk

Frame B:
fnfviikkrnqiiiyiyknikkik*efyi*llfyf*lhyiyhqllvgvvaeivkvivqni
hvnqievvhiyhlyhvvvrknfsvliviieivvmiyhvlktrelvkslrfgqvvnhieil
yhiihqiqphvnh*vvkqevwnvnglnhhhvmellvaqefqdvlvimvavinvte*d---

---XXCXXDXSCKMIRGNXTCIKARPAVPPPHCSTCAELSSACNHVGMICIQVPSNCTNT
RFPCCPSHPICIHPSTTAASTIATTASTVATTTSATTAGTTTGGTTTGGSTSDSSAASSA
DSSAASSSPSSSAASSAASSEPSSSAASSSAPSSASSSAPSSASSSAPSSSASSSAASSA
ASSESXRK

Frame C:
sil*l*kkgik*lyiyiki*kk*nenfifsfsfifnyiisitnfwlgwwqrl*kssfriy
m*ir*klcifticimlw*eriflc*s*s*kll**fimc*khenw*nl*dlvkl*tt*rfc
tisftkynhm*iirl*skrygm*mg*iitmlwnfllpknskmcwsswwrs*m*qnem---

---xnvxxixlvk*levxqlvlkqdqryhhhiaqlvqsyhqhvitle*fvfkyqaivpil
dshvahliqfvfihqllllqplqqlhqlsqlqplqllqvqqlvelqlvvqllivvllpqp
ivalpphhhqavllqvplqvnhqvaplqvvlhpllqvalhplpqvvlhhhqlqvqlhhql
qaqkvxe
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFM174 (AFM174Q) /CSM/AF/AFM1-D/AFM174Q.Seq.d/                       1923   0.0
SFJ689 (SFJ689Q) /CSM/SF/SFJ6-D/SFJ689Q.Seq.d/                       1049   0.0
SFF642 (SFF642Q) /CSM/SF/SFF6-B/SFF642Q.Seq.d/                       1049   0.0
SFK842 (SFK842Q) /CSM/SF/SFK8-B/SFK842Q.Seq.d/                       1043   0.0
SFE171 (SFE171Q) /CSM/SF/SFE1-C/SFE171Q.Seq.d/                       1043   0.0
CHL889 (CHL889Q) /CSM/CH/CHL8-D/CHL889Q.Seq.d/                       1043   0.0
CFK438 (CFK438Q) /CSM/CF/CFK4-B/CFK438Q.Seq.d/                       1043   0.0
CFJ770 (CFJ770Q) /CSM/CF/CFJ7-C/CFJ770Q.Seq.d/                       1043   0.0
CFJ224 (CFJ224Q) /CSM/CF/CFJ2-A/CFJ224Q.Seq.d/                       1043   0.0
CFJ121 (CFJ121Q) /CSM/CF/CFJ1-A/CFJ121Q.Seq.d/                       1043   0.0
own update 2009. 4. 3
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(D13973) Dictyostelium discoideum gene for Dp87 protein, com...  1035   0.0    4
(BJ346986) Dictyostelium discoideum cDNA clone:dda25c19, 3' ...  1057   0.0    1
(BJ402177) Dictyostelium discoideum cDNA clone:dds20a24, 3' ...  1049   0.0    1
(BJ398391) Dictyostelium discoideum cDNA clone:dds12c12, 3' ...  1049   0.0    1
(BJ402948) Dictyostelium discoideum cDNA clone:dds18e23, 3' ...  1043   0.0    1
(BJ402296) Dictyostelium discoideum cDNA clone:dds16n03, 3' ...  1043   0.0    1
(BJ402285) Dictyostelium discoideum cDNA clone:dds16j01, 3' ...  1043   0.0    1
(BJ402280) Dictyostelium discoideum cDNA clone:dds16h04, 3' ...  1043   0.0    1
(BJ401894) Dictyostelium discoideum cDNA clone:dds19b22, 3' ...  1043   0.0    1
(BJ401144) Dictyostelium discoideum cDNA clone:dds22d12, 3' ...  1043   0.0    1
dna update 2009. 4. 9
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q04503) RecName: Full=Prespore protein Dp87; Flags: Precursor;       353   7e-96
D13973_1(D13973|pid:none) Dictyostelium discoideum gene for Dp87...   349   8e-95
(Q54QX2) RecName: Full=Probable spore coat protein DDB_G0283555;...   131   5e-31
(P15269) RecName: Full=Spore coat protein SP70; AltName: Full=Pr...    97   9e-19
AC117267_7(AC117267|pid:none) Dictyostelium discoideum chromosom...    64   1e-08
B25439(B25439)Beejin protein - slime mold (Dictyostelium discoid...    62   3e-08
(Q6TU45) RecName: Full=Probable spore coat protein sigD; AltName...    53   2e-05
AC117076_49(AC117076|pid:none) Dictyostelium discoideum chromoso...    47   3e-05
AC117076_50(AC117076|pid:none) Dictyostelium discoideum chromoso...    48   5e-04
FJ501956_1(FJ501956|pid:none) Oryza sativa Indica Group keratin ...    40   0.016
protein update 2009. 4. 5
PSORT 

 psg: 0.00  gvh: 0.00  alm: 0.00  top: 0.00  tms: 0.00  mit: 0.00  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	100.0 %: 

>> prediction for AFM174 is

5' end seq. ID AFM174F
5' end seq. 
>AFM174F.Seq
ATTCAATTTTGTAATTATAAAAAAAAGGAATCAAATAATTATATATATATATAAAAATAT
AAAAAAAATAAAATGAGAATTTTATATTTAGCTTCTCTTTTATTTTTAATTACATTATAT
CTATCACCAACTTTTGGTTGGGGTGGTGGCAGAGATTGTGAAAGTCATCGTTCAGAATAT
ACATGTAAATCAGATAGAAGTTGTGCATATTTACCATTTGTATCATGTTGTGGTAAGAAA
GAATTTTTCTGTGTTAATCGTGATCATAGAAATTGTTGTGATGATTTATCATGTGCTAAA
AACACGAGAACTGGTGAAATCTTTGAGATTTGGTCAAGTTGTAAACCACATAGAGATTTT
GTACCATATCATTCACCAAATACAACCACATGTGAATCATTAGGTTGTGAAGCAAGAGGT
ATGGAATGTGAATGGGTTGAATCATCACCATGTTATGGAACTTCTTGTTGCCCAAGAATT
CCAAGATGTGTTGGTCATCATGGTGGCGGTCATAAATGTGACAGAATGAGATGTXXXXXX
XXXX
Length of 5' end seq. 544
3' end seq. ID AFM174Z
3' end seq. 
>AFM174Z.Seq
XXXXXXXXXXTNNTNAATGTNCANNTGATTTNTCTTGTAAAATGATTAGAGGTAATNCAA
CTTGTATTAAAGCAAGACCAGCGGTACCACCACCACATTGCTCAACTTGTGCAGAGCTAT
CATCAGCATGTAATCACGTTGGAATGATTTGTATTCAAGTACCAAGCAATTGTACCAATA
CTAGATTCCCATGTTGCCCATCTCATCCAATTTGTATTCATCCATCAACTACTGCTGCTT
CAACCATTGCAACAACTGCATCAACTGTCGCAACTACAACCTCTGCAACTACTGCAGGTA
CAACAACTGGTGGAACTACAACTGGTGGTTCAACTTCTGATAGTAGTGCTGCTTCCTCAG
CCGATAGTAGCGCTGCCTCCTCATCACCATCAAGCAGTGCTGCTTCAAGTGCCGCTTCAA
GTGAACCATCAAGTAGCGCCGCTTCAAGTAGTGCTCCATCCTCTGCTTCAAGTAGCGCTC
CATCCTCTGCCTCAAGTAGTGCTCCATCATCATCAGCTTCAAGTTCAGCTGCATCATCAG
CTGCAAGCTCAGAAAGTCNCAGAAAG
Length of 3' end seq. 566
Connected seq. ID AFM174P
Connected seq. 
>AFM174P.Seq
ATTCAATTTTGTAATTATAAAAAAAAGGAATCAAATAATTATATATATATATAAAAATAT
AAAAAAAATAAAATGAGAATTTTATATTTAGCTTCTCTTTTATTTTTAATTACATTATAT
CTATCACCAACTTTTGGTTGGGGTGGTGGCAGAGATTGTGAAAGTCATCGTTCAGAATAT
ACATGTAAATCAGATAGAAGTTGTGCATATTTACCATTTGTATCATGTTGTGGTAAGAAA
GAATTTTTCTGTGTTAATCGTGATCATAGAAATTGTTGTGATGATTTATCATGTGCTAAA
AACACGAGAACTGGTGAAATCTTTGAGATTTGGTCAAGTTGTAAACCACATAGAGATTTT
GTACCATATCATTCACCAAATACAACCACATGTGAATCATTAGGTTGTGAAGCAAGAGGT
ATGGAATGTGAATGGGTTGAATCATCACCATGTTATGGAACTTCTTGTTGCCCAAGAATT
CCAAGATGTGTTGGTCATCATGGTGGCGGTCATAAATGTGACAGAATGAGATGT------
----TNNTNAATGTNCANNTGATTTNTCTTGTAAAATGATTAGAGGTAATNCAACTTGTA
TTAAAGCAAGACCAGCGGTACCACCACCACATTGCTCAACTTGTGCAGAGCTATCATCAG
CATGTAATCACGTTGGAATGATTTGTATTCAAGTACCAAGCAATTGTACCAATACTAGAT
TCCCATGTTGCCCATCTCATCCAATTTGTATTCATCCATCAACTACTGCTGCTTCAACCA
TTGCAACAACTGCATCAACTGTCGCAACTACAACCTCTGCAACTACTGCAGGTACAACAA
CTGGTGGAACTACAACTGGTGGTTCAACTTCTGATAGTAGTGCTGCTTCCTCAGCCGATA
GTAGCGCTGCCTCCTCATCACCATCAAGCAGTGCTGCTTCAAGTGCCGCTTCAAGTGAAC
CATCAAGTAGCGCCGCTTCAAGTAGTGCTCCATCCTCTGCTTCAAGTAGCGCTCCATCCT
CTGCCTCAAGTAGTGCTCCATCATCATCAGCTTCAAGTTCAGCTGCATCATCAGCTGCAA
GCTCAGAAAGTCNCAGAAAG
Length of connected seq. 1090
Full length Seq ID -
Full length Seq. -
Length of full length seq. -