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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFB409
Library VF
(Link to library)
Clone ID VFB409
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16382-1
Original site URL
Representative seq. ID VFB409P
(Link to Original site)
Representative DNA sequence 
>VFB409 (VFB409Q) /CSM/VF/VFB4-A/VFB409Q.Seq.d/
AGTTTTAATAAACAAATATTTAAATATAATACAACAATAAAATGGACGGTGAAGATGTTC
AAGCTTTAGTTATCGATAATGGTTCTGGTATGTGTAAAGCCGGTTTTGCTGGTGATGATG
CCCCACGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGACACACTGGTGTTATGGTCG
GTATGGGTCAAAAAGACTCTTACGTAGGTGATGAAGCCCAATCAAAGAGAGGTATCTTAA
CCCTCAAATACCCAATTGAACACGGTATTGTTACCAACTGGGATGATATGGAAAAAATCT
GGCATCATACTTTCTACAATGAACTCCGTGTTGCTCCAGAAGAACACCCAGTTCTCTTAA
CTGAAGCCCCATTAAATCCAAAGGCCAACAGAGAAAAAATGACCCAAATTATGTTTGAAA
CCTTCAACACCCCAGCCATGTACGTTGCCATTCAAGCTGTCTTATCCTTATATGCCTCTG
GTCGTACCACCGGTATTGTTATGGATTCAGGTGATGGTGTCTCCCACACTGTTCCAATCT
ATGAAGGTTATGCCTTACCACATGCCATCTTACGTTTAGATTTAGCTGGTCGTGATCTTA
CTGATTACATGATGAAAATCTXXXXXXXXXXGTCGTGATCTTACTGATTACATGATGAAA
ATCTTAACTGAACGTGGTTACTCATTCACCACCACTGCTGAAAGAGAAATCGTCAGAGAT
ATCAAAGAAAAATTAGCCTACGTCGCCCTCGACTTTGAAGCTGAAATGCAAACTGCTGCC
TCATCATCAGCCCTCGAAAAATCATACGAATTACCAGACGGTCAAGTCATCACCATCGGT
AATGAACGTTTCCGTTGCCCAGAAGCCCTCTTCCAACCATCATTCTTAGGTATGGAATCA
GCTGGTATCCACGAAACCACCTATAACTCCATCATGAAATGTGATGTTGATATCCGTAAA
GATTTATACGGTAATGTCGTCTTATCTGGTGGTACAACTATGTTCCCAGGTATTGCTGAT
CGTATGAACAAAGAATTAACTGCTTTAGCCCCATCAACCATGAAAATTAAAATCATTGCT
CCACCAGAACGTAAATACTCTGTCTGGATTGGTGGATCTATCTTAGCTTCACTCTCAACT
TTCCAACAAATGTGGATCTCCAAAGAAGAATATGACGAATCAGGTCCATCAATTGTCCAC
AGAAAATGTTTCTAAACAATTAAAACTAGTGATGAAAGTGCTTCTCACACAAACAAATAA
TTTAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
f**tni*i*YNNKMDGEDVQALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHTGVMVG
MGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLT
EAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVSHTVPIY
EGYALPHAILRLDLAGRDLTDYMMKI---

---RDLTDYMMKILTERGYSFTTTAEREIVRDIKEKLAYVALDFEAEMQTAASSSALEKS
YELPDGQVITIGNERFRCPEALFQPSFLGMESAGIHETTYNSIMKCDVDIRKDLYGNVVL
SGGTTMFPGIADRMNKELTALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISK
EEYDESGPSIVHRKCF*tiktsdesashtnk*f
Translated Amino Acid sequence (All Frames) 
Frame A:
sfnkqifkynttikwtvkmfkl*lsimvlvcvkpvllvmmphvlfshqllvvqdtlvlws
vwvkktlt*vmkpnqrevs*psntqlntvllptgmiwkksgiilstmnsvllqkntqfs*
lkph*iqrptekk*pklclkpstpqpctlpfklsypymplvvppvllwiqvmvsptlfqs
mkvmpyhmpsyv*i*lvvillit**ks---

---vvillit**ks*lnvvthsppllkeksseiskkn*ptspstlklkckllphhqpskn
htnyqtvksspsvmnvsvaqkpssnhhs*vwnqlvstkppitps*nvmlisvkiytvmss
ylvvqlcsqvlliv*tkn*ll*phqp*klksllhqnvntlsglvdls*lhsqlsnkcgsp
kknmtnqvhqlstenvskqlklvmkvlltqtnnl

Frame B:
vlinkylniiqq*ngr*rcssfsyr*wfwyv*srfcw**cptccfpincwsskthwcygr
ygskrllrr**spikerylnpqipn*trycyqlg*ygknlasyflq*tpccsrrtpssln
*spikskgqqrkndpnyv*nlqhpshvrchsscliliclwsyhrycygfr*wclphcsnl
*rlclttchltfrfsws*sy*lhden---

---s*sy*lhdenln*twllihhhc*krnrqryqrkislrrprl*s*nanccliisprki
iritrrsshhhr**tfplprsplptiilrygiswyprnhl*lhhem*c*yp*rfir*crl
iwwynyvpryc*syeqrincfspinhen*nhcstrt*ilcldwwiylsftlnfptnvdlq
rri*rirsincpqkmflnn*n***kcfshkqii*

Frame C:
f**tni*i*YNNKMDGEDVQALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHTGVMVG
MGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLT
EAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVSHTVPIY
EGYALPHAILRLDLAGRDLTDYMMKI---

---RDLTDYMMKILTERGYSFTTTAEREIVRDIKEKLAYVALDFEAEMQTAASSSALEKS
YELPDGQVITIGNERFRCPEALFQPSFLGMESAGIHETTYNSIMKCDVDIRKDLYGNVVL
SGGTTMFPGIADRMNKELTALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISK
EEYDESGPSIVHRKCF*tiktsdesashtnk*f
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFB409 (VFB409Q) /CSM/VF/VFB4-A/VFB409Q.Seq.d/                       2448   0.0
VFJ616 (VFJ616Q) /CSM/VF/VFJ6-A/VFJ616Q.Seq.d/                       1257   0.0
VFJ832 (VFJ832Q) /CSM/VF/VFJ8-B/VFJ832Q.Seq.d/                       1249   0.0
VFO858 (VFO858Q) /CSM/VF/VFO8-C/VFO858Q.Seq.d/                       1245   0.0
VFI569 (VFI569Q) /CSM/VF/VFI5-C/VFI569Q.Seq.d/                       1245   0.0
VFI269 (VFI269Q) /CSM/VF/VFI2-C/VFI269Q.Seq.d/                       1245   0.0
VFE460 (VFE460Q) /CSM/VF/VFE4-C/VFE460Q.Seq.d/                       1245   0.0
VFE392 (VFE392Q) /CSM/VF/VFE3-D/VFE392Q.Seq.d/                       1245   0.0
VFE112 (VFE112Q) /CSM/VF/VFE1-A/VFE112Q.Seq.d/                       1245   0.0
VFD651 (VFD651Q) /CSM/VF/VFD6-C/VFD651Q.Seq.d/                       1245   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.  1245  0.0  4
X03281|X03281.1 Dictyostelium discoideum gene for actin A8.     1094  0.0  1
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.  1078  0.0  6
AC115577|AC115577.2 Dictyostelium discoideum chromosome 2 map 4657875-4914984 strain AX4, complete sequence.   898  0.0  11
AC116956|AC116956.2 Dictyostelium discoideum chromosome 2 map 1418423-1684967 strain AX4, complete sequence.   884  0.0  8
X03283|X03283.1 Dictyostelium discoideum gene for actin A3-S1.   650  0.0  2
M14146|M14146.1 D.discoideum actin 15 gene, complete cds.       1025  0.0  2
AC115579|AC115579.2 Dictyostelium discoideum chromosome 2 map 4915084-5005461 strain AX4, complete sequence.  1025  0.0  4
U25660|U25660.1 Dictyostelium discoideum actin gene, partial cds.   989  0.0  1
AF508263|AF508263.1 Pyrocystis lunula actin mRNA, complete cds.   591  0.0  3
dna update 2003. 8.29
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P53689) RecName: Full=Actin;  &A73764_1(A73764|pid:none) &S7037...   369   0.0
(Q9Y707) RecName: Full=Actin-2;  &AF156258_1(AF156258|pid:none) ...   367   0.0
(Q9Y702) RecName: Full=Actin-1; AltName: Full=Beta-actin;  &AB02...   369   0.0
(Q9UVX4) RecName: Full=Actin;  &AB034637_1(AB034637|pid:none)         370   0.0
EF544569_1(EF544569|pid:none) Amanita muscaria actin (act2) mRNA...   361   0.0
AB115328_1(AB115328|pid:none) Phanerochaete chrysosporium act mR...   364   0.0
EU399629_1(EU399629|pid:none) Cryptococcus albidus strain CBS142...   346   0.0
(Q54HF1) RecName: Full=Putative actin-24;                             329   e-179
(Q554S6) RecName: Full=Actin-17; AltName: Full=Actin-2-sub 2;  &...   330   e-178
(P27132) RecName: Full=Actin-2; AltName: Full=Actin II; Flags: F...   350   e-176
protein update 2009. 6.17
PSORT 

 psg: 0.75  gvh: 0.34  alm: 0.42  top: 0.53  tms: 0.00  mit: 0.15  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 36.0 %: nuclear
	 20.0 %: cytoskeletal
	  4.0 %: peroxisomal

>> prediction for VFB409 is cyt
5' end seq. ID VFB409F
5' end seq. 
>VFB409F.Seq
AGTTTTAATAAACAAATATTTAAATATAATACAACAATAAAATGGACGGTGAAGATGTTC
AAGCTTTAGTTATCGATAATGGTTCTGGTATGTGTAAAGCCGGTTTTGCTGGTGATGATG
CCCCACGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGACACACTGGTGTTATGGTCG
GTATGGGTCAAAAAGACTCTTACGTAGGTGATGAAGCCCAATCAAAGAGAGGTATCTTAA
CCCTCAAATACCCAATTGAACACGGTATTGTTACCAACTGGGATGATATGGAAAAAATCT
GGCATCATACTTTCTACAATGAACTCCGTGTTGCTCCAGAAGAACACCCAGTTCTCTTAA
CTGAAGCCCCATTAAATCCAAAGGCCAACAGAGAAAAAATGACCCAAATTATGTTTGAAA
CCTTCAACACCCCAGCCATGTACGTTGCCATTCAAGCTGTCTTATCCTTATATGCCTCTG
GTCGTACCACCGGTATTGTTATGGATTCAGGTGATGGTGTCTCCCACACTGTTCCAATCT
ATGAAGGTTATGCCTTACCACATGCCATCTTACGTTTAGATTTAGCTGGTCGTGATCTTA
CTGATTACATGATGAAAATCT----------
Length of 5' end seq. 621
3' end seq. ID VFB409Z
3' end seq. 
>VFB409Z.Seq
----------GTCGTGATCTTACTGATTACATGATGAAAATCTTAACTGAACGTGGTTAC
TCATTCACCACCACTGCTGAAAGAGAAATCGTCAGAGATATCAAAGAAAAATTAGCCTAC
GTCGCCCTCGACTTTGAAGCTGAAATGCAAACTGCTGCCTCATCATCAGCCCTCGAAAAA
TCATACGAATTACCAGACGGTCAAGTCATCACCATCGGTAATGAACGTTTCCGTTGCCCA
GAAGCCCTCTTCCAACCATCATTCTTAGGTATGGAATCAGCTGGTATCCACGAAACCACC
TATAACTCCATCATGAAATGTGATGTTGATATCCGTAAAGATTTATACGGTAATGTCGTC
TTATCTGGTGGTACAACTATGTTCCCAGGTATTGCTGATCGTATGAACAAAGAATTAACT
GCTTTAGCCCCATCAACCATGAAAATTAAAATCATTGCTCCACCAGAACGTAAATACTCT
GTCTGGATTGGTGGATCTATCTTAGCTTCACTCTCAACTTTCCAACAAATGTGGATCTCC
AAAGAAGAATATGACGAATCAGGTCCATCAATTGTCCACAGAAAATGTTTCTAAACAATT
AAAACTAGTGATGAAAGTGCTTCTCACACAAACAAATAATTTAA
Length of 3' end seq. 634
Connected seq. ID VFB409P
Connected seq. 
>VFB409P.Seq
AGTTTTAATAAACAAATATTTAAATATAATACAACAATAAAATGGACGGTGAAGATGTTC
AAGCTTTAGTTATCGATAATGGTTCTGGTATGTGTAAAGCCGGTTTTGCTGGTGATGATG
CCCCACGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGACACACTGGTGTTATGGTCG
GTATGGGTCAAAAAGACTCTTACGTAGGTGATGAAGCCCAATCAAAGAGAGGTATCTTAA
CCCTCAAATACCCAATTGAACACGGTATTGTTACCAACTGGGATGATATGGAAAAAATCT
GGCATCATACTTTCTACAATGAACTCCGTGTTGCTCCAGAAGAACACCCAGTTCTCTTAA
CTGAAGCCCCATTAAATCCAAAGGCCAACAGAGAAAAAATGACCCAAATTATGTTTGAAA
CCTTCAACACCCCAGCCATGTACGTTGCCATTCAAGCTGTCTTATCCTTATATGCCTCTG
GTCGTACCACCGGTATTGTTATGGATTCAGGTGATGGTGTCTCCCACACTGTTCCAATCT
ATGAAGGTTATGCCTTACCACATGCCATCTTACGTTTAGATTTAGCTGGTCGTGATCTTA
CTGATTACATGATGAAAATCT----------GTCGTGATCTTACTGATTACATGATGAAA
ATCTTAACTGAACGTGGTTACTCATTCACCACCACTGCTGAAAGAGAAATCGTCAGAGAT
ATCAAAGAAAAATTAGCCTACGTCGCCCTCGACTTTGAAGCTGAAATGCAAACTGCTGCC
TCATCATCAGCCCTCGAAAAATCATACGAATTACCAGACGGTCAAGTCATCACCATCGGT
AATGAACGTTTCCGTTGCCCAGAAGCCCTCTTCCAACCATCATTCTTAGGTATGGAATCA
GCTGGTATCCACGAAACCACCTATAACTCCATCATGAAATGTGATGTTGATATCCGTAAA
GATTTATACGGTAATGTCGTCTTATCTGGTGGTACAACTATGTTCCCAGGTATTGCTGAT
CGTATGAACAAAGAATTAACTGCTTTAGCCCCATCAACCATGAAAATTAAAATCATTGCT
CCACCAGAACGTAAATACTCTGTCTGGATTGGTGGATCTATCTTAGCTTCACTCTCAACT
TTCCAACAAATGTGGATCTCCAAAGAAGAATATGACGAATCAGGTCCATCAATTGTCCAC
AGAAAATGTTTCTAAACAATTAAAACTAGTGATGAAAGTGCTTCTCACACAAACAAATAA
TTTAA
Length of connected seq. 1255
Full length Seq ID -
Full length Seq. -
Length of full length seq. -