>VFB330 (VFB330Q) /CSM/VF/VFB3-B/VFB330Q.Seq.d/
ATAAAAATAAAAATAAAATAAAATGAAATTAGATCCAAAAGCATTAAGATACTTAAGTAA
AGATGATTTTAGAACATTAGTTGCAGTTGAAATGGGTATGAAAAATCATGAACTCGTACC
AGTCAGTCTCATTTGCACAATTGCAAATTTAAAATATGGTGGTACTAAAAAATCAATTCA
AACATTACATAAATTTAAATTACTTTTCCATGATGGCAGAAATTATGATGGTTATAAATT
AACATATTTAGGATATGATTTTTTAGCATTAAAAACATTGGTATCAAGAGGAGTATGTTC
ATATGTTGGTAATCAAATTGGTGTTGGTAAAGAATCGGATATTTATATAGTTGCCAATGA
CGATAATCAAGAGATGGTATTAAAATTACATAGATTGGGTAGAGTTTCATTCAAGACAAT
TAAGAACAATCGTGACTATTTGAAGCACAGAAAATCAGCATCATGGCTATACTTGTCAAG
ATTGGCGGCACXXXXXXXXXXCANGTCATTTAAATGCTGAAATGTATTTCGATCGTGATG
TAACTTGTATTCGTGTATTTTTCGAGAAAAGATTTAATTTCATTGGTGAACGTTGGCCAA
AATTTAAAGATTGTCAAAATATTCATTCTTTAGATGTTGAAATATCTGCTTCAGGTTTCA
CTCGTGATATGGAAAAGGAATTTCAAGATTTAAGTAAACAACAAGATGATGATGAAATTA
ATAATAATGATAGTGATGAAAATGATGAAGATGATGATGAAGATGATGATGATGATGATG
AAAATAATGATGATGATGAAAATAATGATAATAATATTGAAAATGAAAATGAAGAAAATA
TTAAAAAATCATCAAAAGTAAATAAAAAAGAAGTTGGAAAGGAAATTAAAAAAGAAGAGG
AGGAAAATAATAACTCTGAAGAGGAGGATGAAGAAGAAGAAGAAGAGGAAGATTCAGATA
GTGAAGAATCTGTTGAAGACAATACACCAGTAATTGAAGAATTTAAAGGTTTATCATTAA
ACAAAAATAGAGATTTCAAATTTTCAAACTTAAAAGATGAAGATCAAAATGCACCAATTG
TATTAAGAAAAGAGTTTATAGAGAATCTTGANAAAATCAACCAACGAACCAAAGAAAGAG
AAAGTNAATTAACNAAGAAGAGAA

*k*k*NKMKLDPKALRYLSKDDFRTLVAVEMGMKNHELVPVSLICTIANLKYGGTKKSIQ
TLHKFKLLFHDGRNYDGYKLTYLGYDFLALKTLVSRGVCSYVGNQIGVGKESDIYIVAND
DNQEMVLKLHRLGRVSFKTIKNNRDYLKHRKSASWLYLSRLAA---

---XHLNAEMYFDRDVTCIRVFFEKRFNFIGERWPKFKDCQNIHSLDVEISASGFTRDME
KEFQDLSKQQDDDEINNNDSDENDEDDDEDDDDDDENNDDDENNDNNIENENEENIKKSS
KVNKKEVGKEIKKEEEENNNSEEEDEEEEEEEDSDSEESVEDNTPVIEEFKGLSLNKNRD
FKFSNLKDEDQNAPIVLRKEFIENLXKINQRTKERESXLTKKR

Frame A:
ikikik*neirsksikilk*r*f*niscs*ngyeks*trtsqshlhnckfkiwwy*kins
nit*i*itfp*wqkl*wl*inifri*ffsiknigikrsmficw*snwcw*rigylyscq*
r*srdgikit*ig*sfiqdn*eqs*lfeaqkisimailvkigg---

---xvi*mlkcisivm*lvfvyfsrkdlislvnvgqnlkivkifil*mlkyllqvslviw
krnfki*vnnkmmmkliimivmkmmkmmmkmmmmmmkimmmmkimiiilkmkmkkilknh
qk*ikkklerklkkkrrkiitlkrrmkkkkkrkiqivknllktihq*lknlkvyh*tkie
isnfqt*kmkikmhqly*eksl*rilxkstnepkkekvn*xrre

Frame B:
*k*k*NKMKLDPKALRYLSKDDFRTLVAVEMGMKNHELVPVSLICTIANLKYGGTKKSIQ
TLHKFKLLFHDGRNYDGYKLTYLGYDFLALKTLVSRGVCSYVGNQIGVGKESDIYIVAND
DNQEMVLKLHRLGRVSFKTIKNNRDYLKHRKSASWLYLSRLAA---

---xsfkc*nvfrs*cnlyscifreki*fhw*tlaki*rlskysffrc*nicfrfhs*yg
kgisrfk*ttr***n*******k**r***r******k*****k****y*k*k*rky*kii
ksk*krswkgn*krrggk**l*rgg*rrrrrgrfr**ric*rqytsn*ri*rfiikqk*r
fqifklkr*rskctncikkrvyres*xnqptnqrkrkxinxee

Frame C:
knknkik*n*iqkh*dt*vkmileh*lqlkwv*kimnsyqsvsfaqlqi*nmvvlknqfk
hyinlnyfsmmaeimmvin*hi*dmif*h*khwyqeeyvhmlviklvlvknrifi*lpmt
iikrwy*nyidwvefhsrqlrtivti*stenqhhgytcqdwrh---

---XHLNAEMYFDRDVTCIRVFFEKRFNFIGERWPKFKDCQNIHSLDVEISASGFTRDME
KEFQDLSKQQDDDEINNNDSDENDEDDDEDDDDDDENNDDDENNDNNIENENEENIKKSS
KVNKKEVGKEIKKEEEENNNSEEEDEEEEEEEDSDSEESVEDNTPVIEEFKGLSLNKNRD
FKFSNLKDEDQNAPIVLRKEFIENLXKINQRTKERESXLTKKR

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFB330 (VFB330Q) /CSM/VF/VFB3-B/VFB330Q.Seq.d/                       1285   0.0
SFG578 (SFG578Q) /CSM/SF/SFG5-D/SFG578Q.Seq.d/                         40   0.074
AHA868 (AHA868Q) /CSM/AH/AHA8-C/AHA868Q.Seq.d/                         40   0.074
VSI707 (VSI707Q) /CSM/VS/VSI7-A/VSI707Q.Seq.d/                         36   1.2
SLF745 (SLF745Q) /CSM/SL/SLF7-B/SLF745Q.Seq.d/                         36   1.2
SLF606 (SLF606Q) /CSM/SL/SLF6-A/SLF606Q.Seq.d/                         36   1.2
SLF592 (SLF592Q) /CSM/SL/SLF5-D/SLF592Q.Seq.d/                         36   1.2
SLE791 (SLE791Q) /CSM/SL/SLE7-D/SLE791Q.Seq.d/                         36   1.2
SLD503 (SLD503Q) /CSM/SL/SLD5-A/SLD503Q.Seq.d/                         36   1.2
SLC682 (SLC682Q) /CSM/SL/SLC6-D/SLC682Q.Seq.d/                         36   1.2

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AL035476|AL035476.7 Plasmodium falciparum MAL4P3.                 88  6e-20  8
BD281706|BD281706.1 Flea head, nerve cord, hindgut and malpighian tubule nucleic acid molecules, proteins and uses thereof.    58  3e-09  2
AJ275272|AJ275272.1 Sus scrofa EST clone RIIIH1.                  58  3e-04  1
BJ043131|BJ043131.1 Xenopus laevis cDNA clone:XL033o18, 5' end, single read.    36  0.009  3
BJ043085|BJ043085.1 Xenopus laevis cDNA clone:XL033m13, 5' end, single read.    36  0.010  3
BJ037982|BJ037982.1 Xenopus laevis cDNA clone:XL042c05, 5' end, single read.    36  0.010  3
BQ384688|BQ384688.1 NISC_mn08b04.y1 NICHD XGC Ov1 Xenopus laevis cDNA clone IMAGE:5049175 5', mRNA sequence.    36  0.011  3
BJ065359|BJ065359.1 Xenopus laevis cDNA clone:XL082l16, 5' end, single read.    36  0.013  3
BU908304|BU908304.1 AGENCOURT_10489671 NICHD XGC Emb1 Xenopus laevis cDNA clone IMAGE:6633695 5', mRNA sequence.    36  0.021  3
AJ453299|AJ453299.1 Gallus gallus EST, clone library riken1, clone 33o6r1.    50  0.064  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54T05) RecName: Full=Serine/threonine-protein kinase rio2;    ...   312   2e-83
AK073376_1(AK073376|pid:none) Oryza sativa Japonica Group cDNA c...   209   1e-52
BT038255_1(BT038255|pid:none) Zea mays full-length cDNA clone ZM...   209   1e-52
EU952236_1(EU952236|pid:none) Zea mays clone 1235111 serine/thre...   209   1e-52
CR753867_2(CR753867|pid:none) Zebrafish DNA sequence from clone ...   196   2e-48
AY398407_1(AY398407|pid:none) Danio rerio clone RK123A3C08 RIO k...   196   2e-48
BC067141_1(BC067141|pid:none) Danio rerio RIO kinase 2 (yeast), ...   196   2e-48
AJ719586_1(AJ719586|pid:none) Gallus gallus mRNA for hypothetica...   195   3e-48
BC161483_1(BC161483|pid:none) Xenopus tropicalis RIO kinase 2, m...   194   7e-48
BC084165_1(BC084165|pid:none) Xenopus laevis RIO kinase 2 (yeast...   194   7e-48

 psg: 0.41  gvh: 0.47  alm: 0.41  top: 0.53  tms: 0.00  mit: 0.22  mip: 0.00
 nuc: 0.09  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 60.0 %: cytoplasmic
	 36.0 %: nuclear
	  4.0 %: extracellular, including cell wall

>> prediction for VFB330 is cyt

>VFB330F.Seq
ATAAAAATAAAAATAAAATAAAATGAAATTAGATCCAAAAGCATTAAGATACTTAAGTAA
AGATGATTTTAGAACATTAGTTGCAGTTGAAATGGGTATGAAAAATCATGAACTCGTACC
AGTCAGTCTCATTTGCACAATTGCAAATTTAAAATATGGTGGTACTAAAAAATCAATTCA
AACATTACATAAATTTAAATTACTTTTCCATGATGGCAGAAATTATGATGGTTATAAATT
AACATATTTAGGATATGATTTTTTAGCATTAAAAACATTGGTATCAAGAGGAGTATGTTC
ATATGTTGGTAATCAAATTGGTGTTGGTAAAGAATCGGATATTTATATAGTTGCCAATGA
CGATAATCAAGAGATGGTATTAAAATTACATAGATTGGGTAGAGTTTCATTCAAGACAAT
TAAGAACAATCGTGACTATTTGAAGCACAGAAAATCAGCATCATGGCTATACTTGTCAAG
ATTGGCGGCAC----------

>VFB330Z.Seq
----------CANGTCATTTAAATGCTGAAATGTATTTCGATCGTGATGTAACTTGTATT
CGTGTATTTTTCGAGAAAAGATTTAATTTCATTGGTGAACGTTGGCCAAAATTTAAAGAT
TGTCAAAATATTCATTCTTTAGATGTTGAAATATCTGCTTCAGGTTTCACTCGTGATATG
GAAAAGGAATTTCAAGATTTAAGTAAACAACAAGATGATGATGAAATTAATAATAATGAT
AGTGATGAAAATGATGAAGATGATGATGAAGATGATGATGATGATGATGAAAATAATGAT
GATGATGAAAATAATGATAATAATATTGAAAATGAAAATGAAGAAAATATTAAAAAATCA
TCAAAAGTAAATAAAAAAGAAGTTGGAAAGGAAATTAAAAAAGAAGAGGAGGAAAATAAT
AACTCTGAAGAGGAGGATGAAGAAGAAGAAGAAGAGGAAGATTCAGATAGTGAAGAATCT
GTTGAAGACAATACACCAGTAATTGAAGAATTTAAAGGTTTATCATTAAACAAAAATAGA
GATTTCAAATTTTCAAACTTAAAAGATGAAGATCAAAATGCACCAATTGTATTAAGAAAA
GAGTTTATAGAGAATCTTGANAAAATCAACCAACGAACCAAAGAAAGAGAAAGTNAATTA
ACNAAGAAGAGAA

>VFB330P.Seq
ATAAAAATAAAAATAAAATAAAATGAAATTAGATCCAAAAGCATTAAGATACTTAAGTAA
AGATGATTTTAGAACATTAGTTGCAGTTGAAATGGGTATGAAAAATCATGAACTCGTACC
AGTCAGTCTCATTTGCACAATTGCAAATTTAAAATATGGTGGTACTAAAAAATCAATTCA
AACATTACATAAATTTAAATTACTTTTCCATGATGGCAGAAATTATGATGGTTATAAATT
AACATATTTAGGATATGATTTTTTAGCATTAAAAACATTGGTATCAAGAGGAGTATGTTC
ATATGTTGGTAATCAAATTGGTGTTGGTAAAGAATCGGATATTTATATAGTTGCCAATGA
CGATAATCAAGAGATGGTATTAAAATTACATAGATTGGGTAGAGTTTCATTCAAGACAAT
TAAGAACAATCGTGACTATTTGAAGCACAGAAAATCAGCATCATGGCTATACTTGTCAAG
ATTGGCGGCAC----------CANGTCATTTAAATGCTGAAATGTATTTCGATCGTGATG
TAACTTGTATTCGTGTATTTTTCGAGAAAAGATTTAATTTCATTGGTGAACGTTGGCCAA
AATTTAAAGATTGTCAAAATATTCATTCTTTAGATGTTGAAATATCTGCTTCAGGTTTCA
CTCGTGATATGGAAAAGGAATTTCAAGATTTAAGTAAACAACAAGATGATGATGAAATTA
ATAATAATGATAGTGATGAAAATGATGAAGATGATGATGAAGATGATGATGATGATGATG
AAAATAATGATGATGATGAAAATAATGATAATAATATTGAAAATGAAAATGAAGAAAATA
TTAAAAAATCATCAAAAGTAAATAAAAAAGAAGTTGGAAAGGAAATTAAAAAAGAAGAGG
AGGAAAATAATAACTCTGAAGAGGAGGATGAAGAAGAAGAAGAAGAGGAAGATTCAGATA
GTGAAGAATCTGTTGAAGACAATACACCAGTAATTGAAGAATTTAAAGGTTTATCATTAA
ACAAAAATAGAGATTTCAAATTTTCAAACTTAAAAGATGAAGATCAAAATGCACCAATTG
TATTAAGAAAAGAGTTTATAGAGAATCTTGANAAAATCAACCAACGAACCAAAGAAAGAG
AAAGTNAATTAACNAAGAAGAGAA