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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFM162
Library AF
(Link to library)
Clone ID AFM162
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID AFM162P
(Link to Original site)
Representative DNA sequence 
>AFM162 (AFM162Q) /CSM/AF/AFM1-C/AFM162Q.Seq.d/
ACTCAAACATCCACCATCACTTTTTATAGTATTACTACTTTAAAATTTGACCTATTTTTT
ATTTATTAAATTAAATAATTCATTGTAAATTCGATAATAAGGGGATTTAAAAAAGGAATC
ATTTTTTTTCACTCTTTATTTTGTACACTTTAAAAAAAAAAXXXXXXXXXXAGATTCAGG
TGATGGTGTCACCCATATCATTCCAGTCTATGAGGGTTACTCAATCCCACATTTGACTCG
TCGTCTCGATGTTGCCGGTCGTGATGTAACTCGTTACCTCATCAAATTATTATTACTCCG
TGGTTACGCTTTCAATCGTACCGCCGATTTCGAAACCATCCGTCAAATCAAAGAGAAACT
CTGTTATGTCGCATACGATGTTCAACAAGAGATGAAATTGGCCTCAGAGACCACTGTTCT
CGTCGAAAACTACACCCTCCCAGACGGTCGTGTAATCAAAGTTGGCCAAGAGAGATTCCA
AGCCTCTGAAGCCCTCTTCAATCCATCTTTGGTCGATGTCGAAGGTGGTGGTGTCCATGA
ACAATTGTTTGATTGTATCACCAAGGCAGACCGTGATCTTCAACAAGGTTTCTATCAACA
TATCGTCCTTTCTGGTGGTTCCTCTATGTATCCAGGTTTACCATCTCGTCTCGAAAA
sequence update 2009. 4. 3
Translated Amino Acid sequence 
lkhppslfivlll*nltyflfiklnnsl*ir**GDLKKESFFFTLYFVHFKKK---

---DSGDGVTHIIPVYEGYSIPHLTRRLDVAGRDVTRYLIKLLLLRGYAFNRTADFETIR
QIKEKLCYVAYDVQQEMKLASETTVLVENYTLPDGRVIKVGQERFQASEALFNPSLVDVE
GGGVHEQLFDCITKADRDLQQGFYQHIVLSGGSSMYPGLPSRLE
Translated Amino Acid sequence (All Frames) 
Frame A:
tqtstitfysittlkfdlffiy*ik*fivnsiirgfkkgiiffhslfctl*kk---

---rfr*wchpyhssl*gllnptfdsssrccrs*cnslphqiiitpwlrfqsyrrfrnhp
snqretllcrircstrdeiglrdhcsrrklhpprrscnqswpreipsl*splqsifgrcr
rwwcp*tiv*lyhqgrp*sstrflstyrpfwwflyvsrftissrk

Frame B:
lkhppslfivlll*nltyflfiklnnsl*ir**GDLKKESFFFTLYFVHFKKK---

---DSGDGVTHIIPVYEGYSIPHLTRRLDVAGRDVTRYLIKLLLLRGYAFNRTADFETIR
QIKEKLCYVAYDVQQEMKLASETTVLVENYTLPDGRVIKVGQERFQASEALFNPSLVDVE
GGGVHEQLFDCITKADRDLQQGFYQHIVLSGGSSMYPGLPSRLE

Frame C:
snihhhfl*yyyfki*pifylln*iihckfdnkgi*krnhffslfilytlkkk---

---iqvmvspisfqsmrvtqshi*lvvsmlpvvm*lvtssnyyysvvtlsivppiskpsv
kskrnsvmshtmfnkr*nwpqrplfsskttpsqtvv*sklakrdskplkpssihlwsmsk
vvvsmnnclivsprqtvifnkvsinissflvvplciqvyhlvsk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SLB194 (SLB194Q) /CSM/SL/SLB1-D/SLB194Q.Seq.d/                        963   0.0
FC-AH01 (FC-AH01Q) /CSM/FC/FC-AH/FC-AH01Q.Seq.d/                      963   0.0
FC-131 (FC-131Q) /CSM/CG/CG-FC/FC-131Q.Seq.d/                         963   0.0
AFM162 (AFM162Q) /CSM/AF/AFM1-C/AFM162Q.Seq.d/                        963   0.0
AFC587 (AFC587Q) /CSM/AF/AFC5-D/AFC587Q.Seq.d/                        963   0.0
VFD448 (VFD448Q) /CSM/VF/VFD4-B/VFD448Q.Seq.d/                        950   0.0
SHK686 (SHK686Q) /CSM/SH/SHK6-D/SHK686Q.Seq.d/                        948   0.0
AFJ309 (AFJ309Q) /CSM/AF/AFJ3-A/AFJ309Q.Seq.d/                        948   0.0
CFH233 (CFH233Q) /CSM/CF/CFH2-B/CFH233Q.Seq.d/                        944   0.0
AFJ315 (AFJ315Q) /CSM/AF/AFJ3-A/AFJ315Q.Seq.d/                        942   0.0
own update 2009. 4. 3
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AF095929) Dictyostelium discoideum actin related protein 2 ...   963   0.0    3
(AC116963) Dictyostelium discoideum chromosome 2 map 4657875...   963   0.0    4
(AU033584) Dictyostelium discoideum slug cDNA, clone SLB194.      963   0.0    1
(BJ346945) Dictyostelium discoideum cDNA clone:dda25k15, 3' ...   963   0.0    1
(BJ341497) Dictyostelium discoideum cDNA clone:dda6m21, 3' e...   963   0.0    1
(BJ430501) Dictyostelium discoideum cDNA clone:ddv7p12, 3' e...   950   0.0    1
(BJ344359) Dictyostelium discoideum cDNA clone:dda19b03, 3' ...   948   0.0    1
(BJ372986) Dictyostelium discoideum cDNA clone:ddc15a10, 3' ...   944   0.0    1
(BJ344408) Dictyostelium discoideum cDNA clone:dda19n03, 3' ...   942   0.0    1
(BJ341712) Dictyostelium discoideum cDNA clone:dda7i20, 3' e...   942   0.0    1
dna update 2009. 4. 9
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(O96621) RecName: Full=Actin-related protein 2; AltName: Full=Ac...   324   1e-87
(P53487) RecName: Full=Actin-related protein 2; AltName: Full=Ac...   256   5e-67
BC047530_1(BC047530|pid:none) Mus musculus ARP2 actin-related pr...   255   9e-67
(Q5BL41) RecName: Full=Actin-related protein 2; AltName: Full=Ac...   255   9e-67
AY891099_1(AY891099|pid:none) Synthetic construct Homo sapiens c...   255   9e-67
(Q5M7U6) RecName: Full=Actin-related protein 2; AltName: Full=Ac...   255   9e-67
BC036253_1(BC036253|pid:none) Homo sapiens ARP2 actin-related pr...   255   9e-67
(P53488) RecName: Full=Actin-related protein 2; AltName: Full=Ac...   255   9e-67
(Q7ZTP2) RecName: Full=Actin-related protein 2-A; AltName: Full=...   255   9e-67
(Q7ZXV3) RecName: Full=Actin-related protein 2-B; AltName: Full=...   254   2e-66
protein update 2009. 4. 5
PSORT 

 psg: 0.00  gvh: 0.00  alm: 0.00  top: 0.00  tms: 0.00  mit: 0.00  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	100.0 %: 

>> prediction for AFM162 is

5' end seq. ID AFM162F
5' end seq. 
>AFM162F.Seq
ACTCAAACATCCACCATCACTTTTTATAGTATTACTACTTTAAAATTTGACCTATTTTTT
ATTTATTAAATTAAATAATTCATTGTAAATTCGATAATAAGGGGATTTAAAAAAGGAATC
ATTTTTTTTCACTCTTTATTTTGTACACTTTAAAAAAAAAAXXXXXXXXXX
Length of 5' end seq. 171
3' end seq. ID AFM162Z
3' end seq. 
>AFM162Z.Seq
XXXXXXXXXXAGATTCAGGTGATGGTGTCACCCATATCATTCCAGTCTATGAGGGTTACT
CAATCCCACATTTGACTCGTCGTCTCGATGTTGCCGGTCGTGATGTAACTCGTTACCTCA
TCAAATTATTATTACTCCGTGGTTACGCTTTCAATCGTACCGCCGATTTCGAAACCATCC
GTCAAATCAAAGAGAAACTCTGTTATGTCGCATACGATGTTCAACAAGAGATGAAATTGG
CCTCAGAGACCACTGTTCTCGTCGAAAACTACACCCTCCCAGACGGTCGTGTAATCAAAG
TTGGCCAAGAGAGATTCCAAGCCTCTGAAGCCCTCTTCAATCCATCTTTGGTCGATGTCG
AAGGTGGTGGTGTCCATGAACAATTGTTTGATTGTATCACCAAGGCAGACCGTGATCTTC
AACAAGGTTTCTATCAACATATCGTCCTTTCTGGTGGTTCCTCTATGTATCCAGGTTTAC
CATCTCGTCTCGAAAA
Length of 3' end seq. 496
Connected seq. ID AFM162P
Connected seq. 
>AFM162P.Seq
ACTCAAACATCCACCATCACTTTTTATAGTATTACTACTTTAAAATTTGACCTATTTTTT
ATTTATTAAATTAAATAATTCATTGTAAATTCGATAATAAGGGGATTTAAAAAAGGAATC
ATTTTTTTTCACTCTTTATTTTGTACACTTTAAAAAAAAAA----------AGATTCAGG
TGATGGTGTCACCCATATCATTCCAGTCTATGAGGGTTACTCAATCCCACATTTGACTCG
TCGTCTCGATGTTGCCGGTCGTGATGTAACTCGTTACCTCATCAAATTATTATTACTCCG
TGGTTACGCTTTCAATCGTACCGCCGATTTCGAAACCATCCGTCAAATCAAAGAGAAACT
CTGTTATGTCGCATACGATGTTCAACAAGAGATGAAATTGGCCTCAGAGACCACTGTTCT
CGTCGAAAACTACACCCTCCCAGACGGTCGTGTAATCAAAGTTGGCCAAGAGAGATTCCA
AGCCTCTGAAGCCCTCTTCAATCCATCTTTGGTCGATGTCGAAGGTGGTGGTGTCCATGA
ACAATTGTTTGATTGTATCACCAAGGCAGACCGTGATCTTCAACAAGGTTTCTATCAACA
TATCGTCCTTTCTGGTGGTTCCTCTATGTATCCAGGTTTACCATCTCGTCTCGAAAA
Length of connected seq. 647
Full length Seq ID -
Full length Seq. -
Length of full length seq. -