>VFB245 (VFB245Q) /CSM/VF/VFB2-B/VFB245Q.Seq.d/
XXXXXXXXXXCCTTTACCACCAGGTGCAAGATTTACATCATCACCAATCTCAACATTCCA
AATTAAAATTTTATCAGCTCGTAATTTAATTGCAGCAGATGTTGGTGGAACTTCAGATCC
TTATGTAAAGATTAAAACACCAGCTACCAATGGCAAAGATTATAAAACTAGACATATTAG
TAAATCATTGAACCCAATTTGGAATGAAACTTTTAATATCGATCTAGGCAATTGCGTCAA
TGATTTAGTCATCATTGAAGTTTACGACCATGATAAAGTGGGTTCCGACGATTTGATTGG
GTTCGTCGCTTTGGACCCCTCCACTTTCCCACGTGGTATTGAAGTTGTCACTACTGAACA
ATTAAGTTTTGTCCCACATGGTGAGATTAACATTGCAATTACTGCAATCAATTTCGGTAG
AGAATTAGTACCACCAACTTATAAATATGACTATGAAAGATGGAGAGCTTCCATTCCATC
AATCTCAAAGAAAGGTAATGAAAAATTAAAAAATAAACAAAAGAAAAATGATCATAAAGC
AAAGAAAAATAAACCATTAGGTATCTATCATAATAAATACCCACCACAAGGTTATTCTAT
CATTA

---LPPGARFTSSPISTFQIKILSARNLIAADVGGTSDPYVKIKTPATNGKDYKTRHISK
SLNPIWNETFNIDLGNCVNDLVIIEVYDHDKVGSDDLIGFVALDPSTFPRGIEVVTTEQL
SFVPHGEINIAITAINFGRELVPPTYKYDYERWRASIPSISKKGNEKLKNKQKKNDHKAK
KNKPLGIYHNKYPPQGYSII

Frame A:
---lyhqvqdlhhhqsqhsklkfyqlvi*lqqmlvelqilm*rlkhqlpmakiikldilv
nh*tqfgmkllisi*aiasmi*sslkfttmikwvpti*lgsslwtpplshvvlklsllnn
*vlshmvrltlqllqsisven*yhqlinmtmkdgelpfhqsqrkvmkn*kinkrkmiikq
rkinh*vsiiinthhkvilsl


Frame B:
---fttrckiyiitnlnipn*nfiss*fncsrcwwnfrslckd*ntsyqwqrl*n*ty**
iiepnle*nf*yrsrqlrq*fshh*slrp**sgfrrfdwvrrfgplhfptwy*schy*ti
kfcptw*d*hcnycnqfr*risttnl*i*l*kmesfhsinlker**kikk*tkek*s*sk
ek*tiryls**ipttrlfyh


Frame C:
---LPPGARFTSSPISTFQIKILSARNLIAADVGGTSDPYVKIKTPATNGKDYKTRHISK
SLNPIWNETFNIDLGNCVNDLVIIEVYDHDKVGSDDLIGFVALDPSTFPRGIEVVTTEQL
SFVPHGEINIAITAINFGRELVPPTYKYDYERWRASIPSISKKGNEKLKNKQKKNDHKAK
KNKPLGIYHNKYPPQGYSII

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFB245 (VFB245Q) /CSM/VF/VFB2-B/VFB245Q.Seq.d/                        971   0.0
VSD693 (VSD693Q) /CSM/VS/VSD6-D/VSD693Q.Seq.d/                         40   0.038
AFL733 (AFL733Q) /CSM/AF/AFL7-B/AFL733Q.Seq.d/                         40   0.038
VFA513 (VFA513Q) /CSM/VF/VFA5-A/VFA513Q.Seq.d/                         38   0.15
VSI765 (VSI765Q) /CSM/VS/VSI7-C/VSI765Q.Seq.d/                         36   0.60
VSB829 (VSB829Q) /CSM/VS/VSB8-B/VSB829Q.Seq.d/                         36   0.60
VSJ546 (VSJ546Q) /CSM/VS/VSJ5-B/VSJ546Q.Seq.d/                         34   2.4
VSA512 (VSA512Q) /CSM/VS/VSA5-A/VSA512Q.Seq.d/                         34   2.4
VFD705 (VFD705Q) /CSM/VF/VFD7-A/VFD705Q.Seq.d/                         34   2.4
VFD505 (VFD505Q) /CSM/VF/VFD5-A/VFD505Q.Seq.d/                         34   2.4

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.   971  0.0  2
AB082542|AB082542.1 Dictyostelium discoideum forA mRNA for formin homology protein A, complete cds.    48  0.13  1
AC130547|AC130547.3 Mus musculus chromosome UNK clone RP24-378G9, WORKING DRAFT SEQUENCE, 4 unordered pieces.    40  0.30  4
D63797|D63797.1 Loligo pealei mRNA for synaptotagmin, complete cds.    46  0.51  1
X72386|X72386.1 L.pealei mRNA for synaptotagmin (partial).        46  0.51  1
AF020408|AF020408.1 Dictyostelium discoideum R2005 mRNA, complete cds.    40  0.78  2
AC124342|AC124342.6 Mus musculus clone RP23-159H24, WORKING DRAFT SEQUENCE, 4 unordered pieces.    44  2.0  1
AQ933599|AQ933599.1 RPCI-23-282N4.TV RPCI-23 Mus musculus genomic clone RPCI-23-282N4, genomic survey sequence.    44  2.0  1
CC844083|CC844083.1 NDL.102J11.T7 Notre Dame Liverpool Aedes aegypti genomic clone NotreDame Liverpool-102J11, genomic survey sequence.    44  2.0  1
BM546991|BM546991.1 AGENCOURT_6499271 NIH_MGC_124 Homo sapiens cDNA clone IMAGE:5729929 5', mRNA sequence.    44  2.0  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC116986_30(AC116986|pid:none) Dictyostelium discoideum chromoso...   355   7e-97
AC115599_39(AC115599|pid:none) Dictyostelium discoideum chromoso...   147   3e-34
AC116920_16(AC116920|pid:none) Dictyostelium discoideum chromoso...   127   2e-28
CR382129_40(CR382129|pid:none) Yarrowia lipolytica strain CLIB12...    75   2e-12
(Q50L43) RecName: Full=Cytosolic phospholipase A2 delta;        ...    72   2e-11
AB195276_1(AB195276|pid:none) Mus musculus Pla2g4d mRNA for cyto...    72   2e-11
CR382136_399(CR382136|pid:none) Debaryomyces hansenii strain CBS...    70   3e-11
(Q9ESF1) RecName: Full=Otoferlin; AltName: Full=Fer-1-like prote...    70   6e-11
(Q9ERC5) RecName: Full=Otoferlin; AltName: Full=Fer-1-like prote...    70   6e-11
AK033317_1(AK033317|pid:none) Mus musculus 15 days embryo male t...    70   6e-11

 psg: 0.87  gvh: 0.42  alm: 0.44  top: 0.47  tms: 0.00  mit: 0.42  mip: 0.05
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: mitochondrial
	 32.0 %: nuclear
	 16.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: peroxisomal

>> prediction for VFB245 is mit

>VFB245Z.Seq
----------CCTTTACCACCAGGTGCAAGATTTACATCATCACCAATCTCAACATTCCA
AATTAAAATTTTATCAGCTCGTAATTTAATTGCAGCAGATGTTGGTGGAACTTCAGATCC
TTATGTAAAGATTAAAACACCAGCTACCAATGGCAAAGATTATAAAACTAGACATATTAG
TAAATCATTGAACCCAATTTGGAATGAAACTTTTAATATCGATCTAGGCAATTGCGTCAA
TGATTTAGTCATCATTGAAGTTTACGACCATGATAAAGTGGGTTCCGACGATTTGATTGG
GTTCGTCGCTTTGGACCCCTCCACTTTCCCACGTGGTATTGAAGTTGTCACTACTGAACA
ATTAAGTTTTGTCCCACATGGTGAGATTAACATTGCAATTACTGCAATCAATTTCGGTAG
AGAATTAGTACCACCAACTTATAAATATGACTATGAAAGATGGAGAGCTTCCATTCCATC
AATCTCAAAGAAAGGTAATGAAAAATTAAAAAATAAACAAAAGAAAAATGATCATAAAGC
AAAGAAAAATAAACCATTAGGTATCTATCATAATAAATACCCACCACAAGGTTATTCTAT
CATTA