>VFB228 (VFB228Q) /CSM/VF/VFB2-B/VFB228Q.Seq.d/
AAAATTTTTTTTTTAATGATTAAAAATGATTAAAAAAAGTGCATTAATAACATTATTTTT
AGTTTCACTAATTTTGGGTGTTTCTTTATCCCAAAAACCATTTTATTGCCAAGCACCAGA
ACCAACACCAAGTTTAAATACAGATGGATTAACATTAAAGATGGTACAAATTTTAACAAG
ACATGGTGATAGAACACCATTGTATAGTACATTAAAACCAACAATGAATACATGGGATTG
TAATTTAGGATGGTTAATGGTATCAAGTTTAAATAATGTACCAGGAGCAGCAACAGATGT
TGATAGATTATTTAGAAAAGTTTATATGCCAAATAGAGAATATTTCCCAGGTAATTGTTC
AGACGGTCAATTGACCTCACTTGGATTCCAACAACATTTACAATTAGGTCAAAGTTTACG
TCAACTCTACGTTGATAAATATGAATTATTACCAAGTGAATTATCAGTGGATGCAGCATC
AACCATTTGGGTTCGTTCAACCGATGTACCAAGAACCATTCAATCGGTTCAAGGTCATTT
AACTGCTTXXXXXXXXXXTATGAAAATATGACACCAAATCCAACTCTTTGCCCAGAGTTG
GCTGTTTTGATCGCCAATACTACCACCACACCAGAGTGGGGGGAATTCATTACCAACACC
ACCCAATTAAAAGAGGATGTTATGGAAACATTGGGTATCTCTGTATTCCCAGGTTGGAGT
TCATTAATGGATTTATTCTTTGCGACTCAATGTCACGATTTCCCATTACCAGAGGGTGTC
ACTCAAGATATGGTCACTCAAGTCTATGAAGCTGCCTATTGGCAATACCAATATCAACTC
TCTTTCCCAATGATTGCTCGTCTTGGTATGTCTACTTTCTTAGAGGAGGTAGTCGATAAT
ATTAGAGCTTTTGTTAATGGTACTTCCTCTGTCAAATATATCGTTTTCTCTGGTCATGAT
GATTCAGTTGGTCCATTCACTAATTTATTTGGTCTCATGAAAGAATGGCCACCTTATGCT
TCACATGTTGAACTTGAATTGTGGTCTGATGAAAAAGATAATTATTTCCTCCAATTTAAA
TTTAATGGTCAAAGTTATACATTAAATGGTTGTGAAGATGTTATGTGCCCAATTNATTCA
TTCTTTGAAACTGCATACTCAATTTTAGTTCCAAATTATGCTGATGCATGTTCAAATTCA
ACAANACATTTAATTTAACAAAAAAACCCTCCGATTTCT

kfff**LKMIKKSALITLFLVSLILGVSLSQKPFYCQAPEPTPSLNTDGLTLKMVQILTR
HGDRTPLYSTLKPTMNTWDCNLGWLMVSSLNNVPGAATDVDRLFRKVYMPNREYFPGNCS
DGQLTSLGFQQHLQLGQSLRQLYVDKYELLPSELSVDAASTIWVRSTDVPRTIQSVQGHL
TA---

---YENMTPNPTLCPELAVLIANTTTTPEWGEFITNTTQLKEDVMETLGISVFPGWSSLM
DLFFATQCHDFPLPEGVTQDMVTQVYEAAYWQYQYQLSFPMIARLGMSTFLEEVVDNIRA
FVNGTSSVKYIVFSGHDDSVGPFTNLFGLMKEWPPYASHVELELWSDEKDNYFLQFKFNG
QSYTLNGCEDVMCPIXSFFETAYSILVPNYADACSNSTXHLI*qknppis

Frame A:
kifflmiknd*kkcinniifsftnfgcffipktillpstrtntkfkyrwinikdgtnfnk
tw**ntiv*yiktnneymgl*frmvngikfk*ctrssnrc**ii*kslyak*rifpr*lf
rrsidltwipttftirskftstlr**i*iitk*iisgcsinhlgsfnrctknhsigsrsf
nc---

---YENMTPNPTLCPELAVLIANTTTTPEWGEFITNTTQLKEDVMETLGISVFPGWSSLM
DLFFATQCHDFPLPEGVTQDMVTQVYEAAYWQYQYQLSFPMIARLGMSTFLEEVVDNIRA
FVNGTSSVKYIVFSGHDDSVGPFTNLFGLMKEWPPYASHVELELWSDEKDNYFLQFKFNG
QSYTLNGCEDVMCPIXSFFETAYSILVPNYADACSNSTXHLI*qknppis

Frame B:
kfff**LKMIKKSALITLFLVSLILGVSLSQKPFYCQAPEPTPSLNTDGLTLKMVQILTR
HGDRTPLYSTLKPTMNTWDCNLGWLMVSSLNNVPGAATDVDRLFRKVYMPNREYFPGNCS
DGQLTSLGFQQHLQLGQSLRQLYVDKYELLPSELSVDAASTIWVRSTDVPRTIQSVQGHL
TA---

---mki*hqiqlfaqswlf*spilpphqsggnslptppn*krmlwkhwvslysqvgvh*w
iyslrlnvtishyqrvslkiwslksmklpigntninslsq*llvlvclls*rr*siilel
llmvlplsnisfslvmmiqlvhsliylvs*knghlmlhmlnlncglmkkiiissnlnlmv
kvih*mvvkmlcaqxihslklhtqf*fqimlmhvqiqqxi*fnkktlrf

Frame C:
nfffnd*k*lkkvh**hyf*fh*fwvflypknhfiakhqnqhqv*iqmd*h*rwykf*qd
mviehhcivh*nqq*ihgivi*dg*wyqv*imyqeqqqmlidylekficqienisqvivq
tvn*phldsnniyn*vkvyvnstlinmnyyqvnyqwmqhqpfgfvqpmyqepfnrfkvi*
ll---

---*kydtksnslprvgcfdrqyyhhtrvggihyqhhpikrgcygnigylciprlefing
filcdsmsrfpitrgchsryghssl*scllaipistlfpndcsswyvyflrggsr*y*sf
c*wyflcqiyrflws**fswsih*fiwshermatlcftc*t*ivv**kr*lfppi*i*ws
klyikwl*rcyvpnxfil*ncilnfssklc*cmfkfnxtfnltkkpsdf

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFB228 (VFB228Q) /CSM/VF/VFB2-B/VFB228Q.Seq.d/                       2276   0.0
CFA225 (CFA225Q) /CSM/CF/CFA2-B/CFA225Q.Seq.d/                       1291   0.0
VFB675 (VFB675Q) /CSM/VF/VFB6-D/VFB675Q.Seq.d/                       1277   0.0
AFO855 (AFO855Q) /CSM/AF/AFO8-C/AFO855Q.Seq.d/                       1277   0.0
VFB114 (VFB114Q) /CSM/VF/VFB1-A/VFB114Q.Seq.d/                       1273   0.0
VFM810 (VFM810Q) /CSM/VF/VFM8-A/VFM810Q.Seq.d/                       1267   0.0
VFG714 (VFG714Q) /CSM/VF/VFG7-A/VFG714Q.Seq.d/                       1267   0.0
CHD582 (CHD582Q) /CSM/CH/CHD5-D/CHD582Q.Seq.d/                       1267   0.0
SFI824 (SFI824Q) /CSM/SF/SFI8-A/SFI824Q.Seq.d/                       1265   0.0
VFG445 (VFG445Q) /CSM/VF/VFG4-B/VFG445Q.Seq.d/                       1263   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

BH164673|BH164673.1 ENTTE80TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    38  0.60  2
AZ544963|AZ544963.1 ENTEB40TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    38  0.60  2
AC145042|AC145042.2 Macropus eugenii clone ME_KBa-289E24, WORKING DRAFT SEQUENCE, 4 ordered pieces.    38  1.6  3
AC144964|AC144964.1 Silurana tropicalis clone ISB-343A1, WORKING DRAFT SEQUENCE, 5 unordered pieces.    40  2.0  3
AC132034|AC132034.4 Rattus norvegicus clone CH230-401A9, WORKING DRAFT SEQUENCE, 4 unordered pieces.    44  4.2  1
BV062901|BV062901.1 S212P6423FB4.T0 CZECHII/Ei Mus musculus STS genomic, sequence tagged site.    44  4.2  1
AC117184|AC117184.2 Mus musculus chromosome UNK clone RP23-37L10, WORKING DRAFT SEQUENCE, 5 unordered pieces.    44  4.2  1
AC093321|AC093321.1 Homo sapiens chromosome UNK clone RP11-225G3, WORKING DRAFT SEQUENCE, 3 unordered pieces.    44  4.2  1
AC092792|AC092792.2 Homo sapiens chromosome UNK clone RP11-806O14, *** SEQUENCING IN PROGRESS ***, 4 unordered pieces.    44  4.2  1
BH151597|BH151597.1 ENTPW89TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    44  4.2  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54PR9) RecName: Full=Counting factor 60; Flags: Precursor;  &D...   451   e-125
BC154980_1(BC154980|pid:none) Xenopus laevis hypothetical protei...    65   3e-18
AM270193_80(AM270193|pid:none) Aspergillus niger contig An09c004...    56   4e-15
AM920431_449(AM920431|pid:none) Penicillium chrysogenum Wisconsi...    70   8e-15
(Q0P5F0) RecName: Full=Lysosomal acid phosphatase;          Shor...    57   8e-14
CT030733_1(CT030733|pid:none) Mouse DNA sequence from clone RP23...    57   2e-13
DQ150475_1(DQ150475|pid:none) Macaca mulatta prostatic acid phos...    60   2e-13
(Q8CE08) RecName: Full=Prostatic acid phosphatase;          EC=3...    57   2e-13
AF210243_1(AF210243|pid:none) Mus musculus prostatic acid phosph...    56   4e-13
BC090765_1(BC090765|pid:none) Danio rerio zgc:113200, mRNA (cDNA...    52   1e-12

 psg: 0.97  gvh: 0.80  alm: 0.38  top: 0.27  tms: 0.00  mit: 0.38  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 64.0 %: extracellular, including cell wall
	 12.0 %: endoplasmic reticulum
	  8.0 %: vacuolar
	  8.0 %: mitochondrial
	  4.0 %: cytoplasmic
	  4.0 %: nuclear

>> prediction for VFB228 is exc

>VFB228F.Seq
AAAATTTTTTTTTTAATGATTAAAAATGATTAAAAAAAGTGCATTAATAACATTATTTTT
AGTTTCACTAATTTTGGGTGTTTCTTTATCCCAAAAACCATTTTATTGCCAAGCACCAGA
ACCAACACCAAGTTTAAATACAGATGGATTAACATTAAAGATGGTACAAATTTTAACAAG
ACATGGTGATAGAACACCATTGTATAGTACATTAAAACCAACAATGAATACATGGGATTG
TAATTTAGGATGGTTAATGGTATCAAGTTTAAATAATGTACCAGGAGCAGCAACAGATGT
TGATAGATTATTTAGAAAAGTTTATATGCCAAATAGAGAATATTTCCCAGGTAATTGTTC
AGACGGTCAATTGACCTCACTTGGATTCCAACAACATTTACAATTAGGTCAAAGTTTACG
TCAACTCTACGTTGATAAATATGAATTATTACCAAGTGAATTATCAGTGGATGCAGCATC
AACCATTTGGGTTCGTTCAACCGATGTACCAAGAACCATTCAATCGGTTCAAGGTCATTT
AACTGCTT----------

>VFB228Z.Seq
----------TATGAAAATATGACACCAAATCCAACTCTTTGCCCAGAGTTGGCTGTTTT
GATCGCCAATACTACCACCACACCAGAGTGGGGGGAATTCATTACCAACACCACCCAATT
AAAAGAGGATGTTATGGAAACATTGGGTATCTCTGTATTCCCAGGTTGGAGTTCATTAAT
GGATTTATTCTTTGCGACTCAATGTCACGATTTCCCATTACCAGAGGGTGTCACTCAAGA
TATGGTCACTCAAGTCTATGAAGCTGCCTATTGGCAATACCAATATCAACTCTCTTTCCC
AATGATTGCTCGTCTTGGTATGTCTACTTTCTTAGAGGAGGTAGTCGATAATATTAGAGC
TTTTGTTAATGGTACTTCCTCTGTCAAATATATCGTTTTCTCTGGTCATGATGATTCAGT
TGGTCCATTCACTAATTTATTTGGTCTCATGAAAGAATGGCCACCTTATGCTTCACATGT
TGAACTTGAATTGTGGTCTGATGAAAAAGATAATTATTTCCTCCAATTTAAATTTAATGG
TCAAAGTTATACATTAAATGGTTGTGAAGATGTTATGTGCCCAATTNATTCATTCTTTGA
AACTGCATACTCAATTTTAGTTCCAAATTATGCTGATGCATGTTCAAATTCAACAANACA
TTTAATTTAACAAAAAAACCCTCCGATTTCT

>VFB228P.Seq
AAAATTTTTTTTTTAATGATTAAAAATGATTAAAAAAAGTGCATTAATAACATTATTTTT
AGTTTCACTAATTTTGGGTGTTTCTTTATCCCAAAAACCATTTTATTGCCAAGCACCAGA
ACCAACACCAAGTTTAAATACAGATGGATTAACATTAAAGATGGTACAAATTTTAACAAG
ACATGGTGATAGAACACCATTGTATAGTACATTAAAACCAACAATGAATACATGGGATTG
TAATTTAGGATGGTTAATGGTATCAAGTTTAAATAATGTACCAGGAGCAGCAACAGATGT
TGATAGATTATTTAGAAAAGTTTATATGCCAAATAGAGAATATTTCCCAGGTAATTGTTC
AGACGGTCAATTGACCTCACTTGGATTCCAACAACATTTACAATTAGGTCAAAGTTTACG
TCAACTCTACGTTGATAAATATGAATTATTACCAAGTGAATTATCAGTGGATGCAGCATC
AACCATTTGGGTTCGTTCAACCGATGTACCAAGAACCATTCAATCGGTTCAAGGTCATTT
AACTGCTT----------TATGAAAATATGACACCAAATCCAACTCTTTGCCCAGAGTTG
GCTGTTTTGATCGCCAATACTACCACCACACCAGAGTGGGGGGAATTCATTACCAACACC
ACCCAATTAAAAGAGGATGTTATGGAAACATTGGGTATCTCTGTATTCCCAGGTTGGAGT
TCATTAATGGATTTATTCTTTGCGACTCAATGTCACGATTTCCCATTACCAGAGGGTGTC
ACTCAAGATATGGTCACTCAAGTCTATGAAGCTGCCTATTGGCAATACCAATATCAACTC
TCTTTCCCAATGATTGCTCGTCTTGGTATGTCTACTTTCTTAGAGGAGGTAGTCGATAAT
ATTAGAGCTTTTGTTAATGGTACTTCCTCTGTCAAATATATCGTTTTCTCTGGTCATGAT
GATTCAGTTGGTCCATTCACTAATTTATTTGGTCTCATGAAAGAATGGCCACCTTATGCT
TCACATGTTGAACTTGAATTGTGGTCTGATGAAAAAGATAATTATTTCCTCCAATTTAAA
TTTAATGGTCAAAGTTATACATTAAATGGTTGTGAAGATGTTATGTGCCCAATTNATTCA
TTCTTTGAAACTGCATACTCAATTTTAGTTCCAAATTATGCTGATGCATGTTCAAATTCA
ACAANACATTTAATTTAACAAAAAAACCCTCCGATTTCT