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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFB219
Library VF
(Link to library)
Clone ID VFB219
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16455-1
Original site URL
Representative seq. ID VFB219P
(Link to Original site)
Representative DNA sequence 
>VFB219 (VFB219Q) /CSM/VF/VFB2-A/VFB219Q.Seq.d/
CACTGGTCATTTAATCTACAAATGTGGTGGTATCGATAAGAGAGTTATCGAAAAGTATGA
AAAGGAAGCTTCTGAAATGGGTAAACAATCCTTCAAATACGCCTGGGTTATGGATAAATT
AAAGGCTGAACGTGAAAGAGGTATCACCATCGATATTGCTTTATGGAAATTCGAAACCTC
CAAATACTACTTCACCATCATTGATGCCCCAGGTCACAGAGATTTCATCAAGAATATGAT
TACTGGTACCTCCCAAGCCGATTGTGCCGTCTTAGTCATTGCCTCCCCAACTGGTGAATT
CGAAGCTGGTATCGCCAAAAACGGTCAAACTCGTGAACACGCTCTCCTTGCTTACACTTT
AGGTGTCAAACAAATGATCGTTGCTATCAACAAGATGGATGAAAAATCAACCAACTACTC
ACAAGCCCGTTACGACGAAXXXXXXXXXXTATTCCATTACAAGATGTCTACAAGATCGGT
GGTATCGGTACTGTTTCCAGTAGGTCGTGTCGAAACTGGTATCATTAAACCAGGTATGGT
CGTCACCTTTGCCCCAGCTGGTCTCTCAACTGAAGTCAAATCAGTCGAAATGCATCACGA
ACAACTCCCAGAAGCCCGTCCAGGTGACAATGTAGGTTTCAACGTTAAAAACGTTTCAGT
CAAAGAAATTAAAAGAGGTATGGTCGCTGGTGACTCCAAAAACGATCCACCACAAGAAAC
TGAAAAATTCGTTGCTCAAGTTATCGTCCTCAACCATCCAGGTCAAATCCATGCTGGTTA
CTCACCAGTCTTAGATTGTCACACTGCTCACATTGCCTGTAAATTCACTGAAATCGTCGA
TAAAGTTGATCGTCGTACTGGTGCCGTCGTTGCCAAAGAAGGTACTGCCGCCGTCGTCTT
AAAGAATGGTGATGCCGCTATGGTCGAATTAACCCCATCTCGTCCAATGTGTGTTGAATC
CTTCACTGAATACCCACCATTAGGTCGTTTCGCCGTCAGAGATATGAGACAAACCGTCGC
CGTCGGTGTCATCAAATCAACCGTCAAGAAAGCCCCAGGTAAAGCCGGTATAAGAAAGGT
GCTGCGCCCCATCAAAAAAGAAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
TGHLIYKCGGIDKRVIEKYEKEASEMGKQSFKYAWVMDKLKAERERGITIDIALWKFETS
KYYFTIIDAPGHRDFIKNMITGTSQADCAVLVIASPTGEFEAGIAKNGQTREHALLAYTL
GVKQMIVAINKMDEKSTNYSQARYDE---

---FHYKMSTRSVVSVLFPVGRVETGIIKPGMVVTFAPAGLSTEVKSVEMHHEQLPEARP
GDNVGFNVKNVSVKEIKRGMVAGDSKNDPPQETEKFVAQVIVLNHPGQIHAGYSPVLDCH
TAHIACKFTEIVDKVDRRTGAVVAKEGTAAVVLKNGDAAMVELTPSRPMCVESFTEYPPL
GRFAVRDMRQTVAVGVIKSTVKKAPGKAGIRKVLRPIKKE
Translated Amino Acid sequence (All Frames) 
Frame A:
hwsfnlqmwwyr*esyrkv*kgsf*ng*tilqirlgyg*ikg*t*kryhhrycfmeirnl
qillhhh*cprsqrfhqeydywylpsrlcrlshclpnw*irswyrqkrsns*trspclhf
rcqtndrcyqqdg*kinqlltsplrr---

---ysitrclqdrwyrycfq*vvsklvslnqvwssplpqlvsqlksnqskcitnnsqkpv
qvtm*vstlktfqskklkevwslvtpktihhkklknsllklssstiqvksmlvthqs*iv
tlltlpvnslkssiklivvlvpslpkkvlppss*rmvmplwsn*phlvqcvlnpslnthh
*vvspsei*dkpspsvssnqpsrkpqvkpv*erccapskkk

Frame B:
TGHLIYKCGGIDKRVIEKYEKEASEMGKQSFKYAWVMDKLKAERERGITIDIALWKFETS
KYYFTIIDAPGHRDFIKNMITGTSQADCAVLVIASPTGEFEAGIAKNGQTREHALLAYTL
GVKQMIVAINKMDEKSTNYSQARYDE---

---iplqdvykiggigtvssrscrnwyh*trygrhlcpswsln*sqisrnasrttprsps
r*qcrfqr*krfsqrn*krygrw*lqkrsttrn*kircssyrpqpsrsnpcwlltslrls
hcshcl*ih*nrr*s*ssywcrrcqrrycrrrlkew*crygrinpissnvc*ilh*ipti
rsfrrqryetnrrrrchqinrqespr*srykkgaaphqkr

Frame C:
lvi*stnvvvsirelsksmkrkllkwvnnpsntpglwin*rlnvkevspsillygnskpp
nttspslmpqvteissri*llvppkpivps*slppqlvnsklvspktvklvntlslltl*
vsnk*sllstrwmknqptthkpvtt---

---FHYKMSTRSVVSVLFPVGRVETGIIKPGMVVTFAPAGLSTEVKSVEMHHEQLPEARP
GDNVGFNVKNVSVKEIKRGMVAGDSKNDPPQETEKFVAQVIVLNHPGQIHAGYSPVLDCH
TAHIACKFTEIVDKVDRRTGAVVAKEGTAAVVLKNGDAAMVELTPSRPMCVESFTEYPPL
GRFAVRDMRQTVAVGVIKSTVKKAPGKAGIRKVLRPIKKE
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFB219 (VFB219Q) /CSM/VF/VFB2-A/VFB219Q.Seq.d/                       2127   0.0
AFJ556 (AFJ556Q) /CSM/AF/AFJ5-C/AFJ556Q.Seq.d/                       1289   0.0
VFO757 (VFO757Q) /CSM/VF/VFO7-C/VFO757Q.Seq.d/                       1281   0.0
VFA633 (VFA633Q) /CSM/VF/VFA6-B/VFA633Q.Seq.d/                       1281   0.0
VFA160 (VFA160Q) /CSM/VF/VFA1-C/VFA160Q.Seq.d/                       1281   0.0
VFK312 (VFK312Q) /CSM/VF/VFK3-A/VFK312Q.Seq.d/                       1275   0.0
VFB450 (VFB450Q) /CSM/VF/VFB4-C/VFB450Q.Seq.d/                       1275   0.0
VFA634 (VFA634Q) /CSM/VF/VFA6-B/VFA634Q.Seq.d/                       1275   0.0
CFH887 (CFH887Q) /CSM/CF/CFH8-D/CFH887Q.Seq.d/                       1275   0.0
CFB512 (CFB512Q) /CSM/CF/CFB5-A/CFB512Q.Seq.d/                       1275   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

X55973|X55973.1 D. discoideum EF1-I gene for elongation factor 1 alpha.  1140  0.0  4
X55972|X55972.1 D. discoideum EF1-II gene for elongation factor 1 alpha.  1092  0.0  4
AF016242|AF016242.1 Dictyostelium discoideum protein synthesis elongation factor 1-alpha (tef2) gene, partial cds.   963  0.0  4
M29935|M29935.1 C.albicans elongation factor 1-alpha (TEF-2) gene, complete cds.   204  e-167  9
M29934|M29934.1 C.albicans elongation factor 1-alpha (TEF-1) gene, complete cds.   192  e-158  9
AX109715|AX109715.1 Sequence 448 from Patent WO0123604.          121  e-150  9
AX109714|AX109714.1 Sequence 447 from Patent WO0123604.          121  e-149  9
AX109679|AX109679.1 Sequence 412 from Patent WO0123604.          161  e-148  9
AX109682|AX109682.1 Sequence 415 from Patent WO0123604.          161  e-147  9
AX109681|AX109681.1 Sequence 414 from Patent WO0123604.          161  e-147  9
dna update 2003. 8.28
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AB174733_1(AB174733|pid:none) Macaca fascicularis brain cDNA clo...   509   e-143
AK297878_1(AK297878|pid:none) Homo sapiens cDNA FLJ52573 complet...   463   e-129
AM055942_137(AM055942|pid:none) Toxoplasma gondii RH, genomic DN...   442   e-122
AY785728_1(AY785728|pid:none) Trypanosoma rangeli strain San Agu...   410   e-113
AY785691_1(AY785691|pid:none) Trypanosoma cruzi strain Esmeraldo...   406   e-112
AY785665_1(AY785665|pid:none) Trypanosoma cruzi strain M6241 cl6...   405   e-111
AY785676_1(AY785676|pid:none) Trypanosoma cruzi strain NR cl3 cl...   405   e-111
AY785685_1(AY785685|pid:none) Trypanosoma cruzi strain Basileu c...   405   e-111
AY785660_1(AY785660|pid:none) Trypanosoma cruzi strain Dm 28 c c...   405   e-111
AY785657_1(AY785657|pid:none) Trypanosoma cruzi strain Colombian...   405   e-111
protein update 2009. 6.17
PSORT 

 psg: 0.54  gvh: 0.25  alm: 0.38  top: 0.53  tms: 0.00  mit: 0.20  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: nuclear
	 44.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: mitochondrial

>> prediction for VFB219 is nuc
5' end seq. ID VFB219F
5' end seq. 
>VFB219F.Seq
CACTGGTCATTTAATCTACAAATGTGGTGGTATCGATAAGAGAGTTATCGAAAAGTATGA
AAAGGAAGCTTCTGAAATGGGTAAACAATCCTTCAAATACGCCTGGGTTATGGATAAATT
AAAGGCTGAACGTGAAAGAGGTATCACCATCGATATTGCTTTATGGAAATTCGAAACCTC
CAAATACTACTTCACCATCATTGATGCCCCAGGTCACAGAGATTTCATCAAGAATATGAT
TACTGGTACCTCCCAAGCCGATTGTGCCGTCTTAGTCATTGCCTCCCCAACTGGTGAATT
CGAAGCTGGTATCGCCAAAAACGGTCAAACTCGTGAACACGCTCTCCTTGCTTACACTTT
AGGTGTCAAACAAATGATCGTTGCTATCAACAAGATGGATGAAAAATCAACCAACTACTC
ACAAGCCCGTTACGACGAA----------
Length of 5' end seq. 439
3' end seq. ID VFB219Z
3' end seq. 
>VFB219Z.Seq
----------TATTCCATTACAAGATGTCTACAAGATCGGTGGTATCGGTACTGTTTCCA
GTAGGTCGTGTCGAAACTGGTATCATTAAACCAGGTATGGTCGTCACCTTTGCCCCAGCT
GGTCTCTCAACTGAAGTCAAATCAGTCGAAATGCATCACGAACAACTCCCAGAAGCCCGT
CCAGGTGACAATGTAGGTTTCAACGTTAAAAACGTTTCAGTCAAAGAAATTAAAAGAGGT
ATGGTCGCTGGTGACTCCAAAAACGATCCACCACAAGAAACTGAAAAATTCGTTGCTCAA
GTTATCGTCCTCAACCATCCAGGTCAAATCCATGCTGGTTACTCACCAGTCTTAGATTGT
CACACTGCTCACATTGCCTGTAAATTCACTGAAATCGTCGATAAAGTTGATCGTCGTACT
GGTGCCGTCGTTGCCAAAGAAGGTACTGCCGCCGTCGTCTTAAAGAATGGTGATGCCGCT
ATGGTCGAATTAACCCCATCTCGTCCAATGTGTGTTGAATCCTTCACTGAATACCCACCA
TTAGGTCGTTTCGCCGTCAGAGATATGAGACAAACCGTCGCCGTCGGTGTCATCAAATCA
ACCGTCAAGAAAGCCCCAGGTAAAGCCGGTATAAGAAAGGTGCTGCGCCCCATCAAAAAA
GAAA
Length of 3' end seq. 654
Connected seq. ID VFB219P
Connected seq. 
>VFB219P.Seq
CACTGGTCATTTAATCTACAAATGTGGTGGTATCGATAAGAGAGTTATCGAAAAGTATGA
AAAGGAAGCTTCTGAAATGGGTAAACAATCCTTCAAATACGCCTGGGTTATGGATAAATT
AAAGGCTGAACGTGAAAGAGGTATCACCATCGATATTGCTTTATGGAAATTCGAAACCTC
CAAATACTACTTCACCATCATTGATGCCCCAGGTCACAGAGATTTCATCAAGAATATGAT
TACTGGTACCTCCCAAGCCGATTGTGCCGTCTTAGTCATTGCCTCCCCAACTGGTGAATT
CGAAGCTGGTATCGCCAAAAACGGTCAAACTCGTGAACACGCTCTCCTTGCTTACACTTT
AGGTGTCAAACAAATGATCGTTGCTATCAACAAGATGGATGAAAAATCAACCAACTACTC
ACAAGCCCGTTACGACGAA----------TATTCCATTACAAGATGTCTACAAGATCGGT
GGTATCGGTACTGTTTCCAGTAGGTCGTGTCGAAACTGGTATCATTAAACCAGGTATGGT
CGTCACCTTTGCCCCAGCTGGTCTCTCAACTGAAGTCAAATCAGTCGAAATGCATCACGA
ACAACTCCCAGAAGCCCGTCCAGGTGACAATGTAGGTTTCAACGTTAAAAACGTTTCAGT
CAAAGAAATTAAAAGAGGTATGGTCGCTGGTGACTCCAAAAACGATCCACCACAAGAAAC
TGAAAAATTCGTTGCTCAAGTTATCGTCCTCAACCATCCAGGTCAAATCCATGCTGGTTA
CTCACCAGTCTTAGATTGTCACACTGCTCACATTGCCTGTAAATTCACTGAAATCGTCGA
TAAAGTTGATCGTCGTACTGGTGCCGTCGTTGCCAAAGAAGGTACTGCCGCCGTCGTCTT
AAAGAATGGTGATGCCGCTATGGTCGAATTAACCCCATCTCGTCCAATGTGTGTTGAATC
CTTCACTGAATACCCACCATTAGGTCGTTTCGCCGTCAGAGATATGAGACAAACCGTCGC
CGTCGGTGTCATCAAATCAACCGTCAAGAAAGCCCCAGGTAAAGCCGGTATAAGAAAGGT
GCTGCGCCCCATCAAAAAAGAAA
Length of connected seq. 1093
Full length Seq ID -
Full length Seq. -
Length of full length seq. -