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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFB215
Library VF
(Link to library)
Clone ID VFB215
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID VFB215P
(Link to Original site)
Representative DNA sequence 
>VFB215 (VFB215Q) /CSM/VF/VFB2-A/VFB215Q.Seq.d/
ATATTTATAATTTATCTTTTTTGTTTAATAAAAAAGAAAGAATACCAACATGAGACTTTT
ATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTATCAACAGTTCATTTCAA
AGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGTGATTGGCATAAAGAAGA
TGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTTGGTGATGAAAATCAAAA
AGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCAGCTAAATTCCCAAGTTT
CAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAGAATGAACAAAAAGTTGA
TTGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGATCAATCAGCATTCGATGG
TGAATCTGAATATTCCATTATGTTTGGTCCAGATGTTTGTGGTGCCTCAAAGAGAGTTCA
CGTCATTCTCAACTACAAAGGAAAGAACCATTTAATCAAGAAAGAAATCAACAAAGTTGA
AACCGATCAATTAACTCATCAATACACTTTGGTCATTTXXXXXXXXXXAAAGGAAATCGA
TGACCCAGAAGATGTTAAACCAGCCGGTCATGACGATATCCCAGCTTCAATCGTTGATCC
AGAAGCTGTCAAACCAGAGGATTGGAATGAAGAAGACGACGGTGAATGGGAAGCCCCAAC
CATCGCCAATCCAGAATACAAAGGTGAATGGAAAGCAAAGAAAATCCCAAATCCAGAATA
CAAAGGTGAATGGGTACACCCATTAATTGATAACCCAGAATACGCTGAAGACAATGAACT
CTACCTCTTTAATGATCTCGGTGCTATCGGTTTCGAATTATGGCAAGTTAAATCTGGTTC
CATCTTTAACAATATGATCGTCNCCGACTCTGTCGAAGAAGCTAAAGATTTCTCTGAAAA
GACCTTTGTTGCCAACCAAGAAGCCGAAAAGAAAATGTTTGACGACCTCGAAGCCGCCAA
AGCTGAAGAGAGAAAGAAAGCTGACGAAAAATTAGCTGCCGAAAAAGCTGCTGAAAAAGA
AGCCGAAGAAGCTGATGAAGAAGAAGAAGAAGTCGCTGAAGAAGATTTAGTTAAAACTAN
GACAAAAAAGAAGAAGTCAAAAAATCAACCAAAAAAGT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
yl*fiffv**KRKNTNMRLLLCLIFLVFVFNFALSTVHFKDTFDNDWESRWVVSDWHKED
GKSGKLVHTAGKWFGDENQKGIQTSEDARFYAVSAKFPSFSNKGKDLVLQYTVKNEQKVD
CGGSYIKLLPSKLDQSAFDGESEYSIMFGPDVCGASKRVHVILNYKGKNHLIKKEINKVE
TDQLTHQYTLVI---

---KEIDDPEDVKPAGHDDIPASIVDPEAVKPEDWNEEDDGEWEAPTIANPEYKGEWKAK
KIPNPEYKGEWVHPLIDNPEYAEDNELYLFNDLGAIGFELWQVKSGSIFNNMIVXDSVEE
AKDFSEKTFVANQEAEKKMFDDLEAAKAEERKKADEKLAAEKAAEKEAEEADEEEEEVAE
EDLVKTXTKKKKSKNQPKK
Translated Amino Acid sequence (All Frames) 
Frame A:
ifiiylfclikkkeyqhetfivfnflsfcfqfciinssfqryl**rlgikmgc**la*rr
w*ir*issycr*mvw**kskrysn**rcsflrcis*ipkfq*qr*rfsspihcke*tks*
lwwfihqvitiqirsisirw*i*ifhyvwsrclwclkessrhsqlqrkepfnqernqqs*
nrsinssihfgh---

---kgnr*prrc*tsrs*rypsfnr*srscqtrgle*rrrr*mgspnhrqsriqr*mesk
enpksriqr*mgtpin**prir*rq*tlpl**srcyrfrimas*iwfhl*qydrxrlcrr
s*rfl*kdlccqprsrkenv*rprsrqs*rekes*rkiscrksc*krsrrs**rrrrsr*
rrfs*nxdkkeevkkstkk

Frame B:
yl*fiffv**KRKNTNMRLLLCLIFLVFVFNFALSTVHFKDTFDNDWESRWVVSDWHKED
GKSGKLVHTAGKWFGDENQKGIQTSEDARFYAVSAKFPSFSNKGKDLVLQYTVKNEQKVD
CGGSYIKLLPSKLDQSAFDGESEYSIMFGPDVCGASKRVHVILNYKGKNHLIKKEINKVE
TDQLTHQYTLVI---

---KEIDDPEDVKPAGHDDIPASIVDPEAVKPEDWNEEDDGEWEAPTIANPEYKGEWKAK
KIPNPEYKGEWVHPLIDNPEYAEDNELYLFNDLGAIGFELWQVKSGSIFNNMIVXDSVEE
AKDFSEKTFVANQEAEKKMFDDLEAAKAEERKKADEKLAAEKAAEKEAEEADEEEEEVAE
EDLVKTXTKKKKSKNQPKK

Frame C:
iynlsflfnkkeript*dfycv*fs*flfsilhyqqfiskiplitignqdgllvigikkm
vnqvn*filqvnglvmkikkvfklvkmlvstlyqlnsqvsvtkvki*fsntl*rmnkkli
vvvhtssyyhpn*inqhsmvnlniplclvqmfvvpqreftsfsttkerti*srkkstklk
pin*lintlwsf---

---rksmtqkmlnqpvmtisqlqsliqklsnqrigmkkttvngkpqpspiqntkvngkqr
ksqiqntkvngyth*litqntlktmnstslmisvlsvsnygklnlvpslti*ssptlskk
lkislkrpllptkkpkrkclttskppklkrerkltkn*lpkkllkkkpkklmkkkkkslk
ki*lklxqkrrsqkinqks
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFB215 (VFB215Q) /CSM/VF/VFB2-A/VFB215Q.Seq.d/                       1965   0.0
AFL337 (AFL337Q) /CSM/AF/AFL3-B/AFL337Q.Seq.d/                       1941   0.0
VFA823 (VFA823Q) /CSM/VF/VFA8-A/VFA823Q.Seq.d/                       1140   0.0
AFE410 (AFE410Q) /CSM/AF/AFE4-A/AFE410Q.Seq.d/                       1140   0.0
AFB848 (AFB848Q) /CSM/AF/AFB8-B/AFB848Q.Seq.d/                       1140   0.0
AFE267 (AFE267Q) /CSM/AF/AFE2-C/AFE267Q.Seq.d/                       1138   0.0
CFA521 (CFA521Q) /CSM/CF/CFA5-A/CFA521Q.Seq.d/                       1136   0.0
AFD843 (AFD843Q) /CSM/AF/AFD8-B/AFD843Q.Seq.d/                       1136   0.0
AFB650 (AFB650Q) /CSM/AF/AFB6-C/AFB650Q.Seq.d/                       1136   0.0
AFE494 (AFE494Q) /CSM/AF/AFE4-D/AFE494Q.Seq.d/                       1134   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

U36937|U36937.1 Dictyostelium discoideum calreticulin mRNA, complete cds.  1122  0.0  2
BD248385|BD248385.1 Gene participating in tolerance against environmental stress.    92  9e-24  3
BH160872|BH160872.1 ENTRI18TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    80  1e-17  3
BI921109|BI921109.1 EST541012 tomato callus Lycopersicon esculentum cDNA clone cLEC72A21 5' end, mRNA sequence.    74  7e-11  2
AZ543757|AZ543757.1 ENTGD24TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    80  7e-11  1
AW649196|AW649196.1 EST327650 tomato germinating seedlings, TAMU Lycopersicon esculentum cDNA clone cLEI7M10 5', mRNA sequence.    74  4e-09  1
AW626316|AW626316.1 EST320223 tomato radicle, 5 d post-imbibition, Cornell University Lycopersicon esculentum cDNA clone cLEZ19K24 5', mRNA sequence.    74  4e-09  1
BE459798|BE459798.1 EST415090 tomato developing/immature green fruit Lycopersicon esculentum cDNA clone cLEM8G13, mRNA sequence.    74  4e-09  1
BE435540|BE435540.1 EST406618 tomato breaker fruit, TIGR Lycopersicon esculentum cDNA clone cLEG27F24, mRNA sequence.    74  4e-09  1
AI771812|AI771812.1 EST252912 tomato ovary, TAMU Lycopersicon esculentum cDNA clone cLED38E1, mRNA sequence.    74  4e-09  1
dna update 2003. 8.28
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U36937_1(U36937|pid:none) Dictyostelium discoideum calreticulin ...   564   e-159
AC133339_28(AC133339|pid:none) Oryza sativa chromosome 3 BAC OSJ...   342   2e-92
AK318734_1(AK318734|pid:none) Arabidopsis thaliana AT1G56340 mRN...   338   3e-91
(Q40401) RecName: Full=Calreticulin; Flags: Precursor;  &T16968(...   338   3e-91
EU984501_1(EU984501|pid:none) Nicotiana tabacum calreticulin mRN...   336   8e-91
AF283816_1(AF283816|pid:none) Pinus taeda calreticulin mRNA, com...   335   2e-90
EF085019_1(EF085019|pid:none) Picea sitchensis clone WS0297_I16 ...   334   4e-90
AB196794_1(AB196794|pid:none) Glycine max Gm crt-1 mRNA for calr...   334   4e-90
(Q9XF98) RecName: Full=Calreticulin; Flags: Precursor;  &AF13473...   332   2e-89
AY836753_1(AY836753|pid:none) Triticum aestivum calreticulin-lik...   332   2e-89
protein update 2009. 6.17
PSORT 

 psg: 1.01  gvh: 0.69  alm: 0.47  top: 0.10  tms: 0.00  mit: 0.40  mip: 0.03
 nuc: 0.10  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: extracellular, including cell wall
	 16.0 %: cytoplasmic
	 12.0 %: mitochondrial
	  8.0 %: Golgi
	  8.0 %: vacuolar
	  8.0 %: nuclear
	  8.0 %: endoplasmic reticulum

>> prediction for VFB215 is exc
5' end seq. ID VFB215F
5' end seq. 
>VFB215F.Seq
ATATTTATAATTTATCTTTTTTGTTTAATAAAAAAGAAAGAATACCAACATGAGACTTTT
ATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTATCAACAGTTCATTTCAA
AGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGTGATTGGCATAAAGAAGA
TGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTTGGTGATGAAAATCAAAA
AGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCAGCTAAATTCCCAAGTTT
CAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAGAATGAACAAAAAGTTGA
TTGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGATCAATCAGCATTCGATGG
TGAATCTGAATATTCCATTATGTTTGGTCCAGATGTTTGTGGTGCCTCAAAGAGAGTTCA
CGTCATTCTCAACTACAAAGGAAAGAACCATTTAATCAAGAAAGAAATCAACAAAGTTGA
AACCGATCAATTAACTCATCAATACACTTTGGTCATTT----------
Length of 5' end seq. 578
3' end seq. ID VFB215Z
3' end seq. 
>VFB215Z.Seq
----------AAAGGAAATCGATGACCCAGAAGATGTTAAACCAGCCGGTCATGACGATA
TCCCAGCTTCAATCGTTGATCCAGAAGCTGTCAAACCAGAGGATTGGAATGAAGAAGACG
ACGGTGAATGGGAAGCCCCAACCATCGCCAATCCAGAATACAAAGGTGAATGGAAAGCAA
AGAAAATCCCAAATCCAGAATACAAAGGTGAATGGGTACACCCATTAATTGATAACCCAG
AATACGCTGAAGACAATGAACTCTACCTCTTTAATGATCTCGGTGCTATCGGTTTCGAAT
TATGGCAAGTTAAATCTGGTTCCATCTTTAACAATATGATCGTCNCCGACTCTGTCGAAG
AAGCTAAAGATTTCTCTGAAAAGACCTTTGTTGCCAACCAAGAAGCCGAAAAGAAAATGT
TTGACGACCTCGAAGCCGCCAAAGCTGAAGAGAGAAAGAAAGCTGACGAAAAATTAGCTG
CCGAAAAAGCTGCTGAAAAAGAAGCCGAAGAAGCTGATGAAGAAGAAGAAGAAGTCGCTG
AAGAAGATTTAGTTAAAACTANGACAAAAAAGAAGAAGTCAAAAAATCAACCAAAAAAGT
Length of 3' end seq. 590
Connected seq. ID VFB215P
Connected seq. 
>VFB215P.Seq
ATATTTATAATTTATCTTTTTTGTTTAATAAAAAAGAAAGAATACCAACATGAGACTTTT
ATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTATCAACAGTTCATTTCAA
AGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGTGATTGGCATAAAGAAGA
TGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTTGGTGATGAAAATCAAAA
AGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCAGCTAAATTCCCAAGTTT
CAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAGAATGAACAAAAAGTTGA
TTGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGATCAATCAGCATTCGATGG
TGAATCTGAATATTCCATTATGTTTGGTCCAGATGTTTGTGGTGCCTCAAAGAGAGTTCA
CGTCATTCTCAACTACAAAGGAAAGAACCATTTAATCAAGAAAGAAATCAACAAAGTTGA
AACCGATCAATTAACTCATCAATACACTTTGGTCATTT----------AAAGGAAATCGA
TGACCCAGAAGATGTTAAACCAGCCGGTCATGACGATATCCCAGCTTCAATCGTTGATCC
AGAAGCTGTCAAACCAGAGGATTGGAATGAAGAAGACGACGGTGAATGGGAAGCCCCAAC
CATCGCCAATCCAGAATACAAAGGTGAATGGAAAGCAAAGAAAATCCCAAATCCAGAATA
CAAAGGTGAATGGGTACACCCATTAATTGATAACCCAGAATACGCTGAAGACAATGAACT
CTACCTCTTTAATGATCTCGGTGCTATCGGTTTCGAATTATGGCAAGTTAAATCTGGTTC
CATCTTTAACAATATGATCGTCNCCGACTCTGTCGAAGAAGCTAAAGATTTCTCTGAAAA
GACCTTTGTTGCCAACCAAGAAGCCGAAAAGAAAATGTTTGACGACCTCGAAGCCGCCAA
AGCTGAAGAGAGAAAGAAAGCTGACGAAAAATTAGCTGCCGAAAAAGCTGCTGAAAAAGA
AGCCGAAGAAGCTGATGAAGAAGAAGAAGAAGTCGCTGAAGAAGATTTAGTTAAAACTAN
GACAAAAAAGAAGAAGTCAAAAAATCAACCAAAAAAGT
Length of connected seq. 1168
Full length Seq ID -
Full length Seq. -
Length of full length seq. -