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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFB213
Library VF
(Link to library)
Clone ID VFB213
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U13856-1
Original site URL
Representative seq. ID VFB213P
(Link to Original site)
Representative DNA sequence 
>VFB213 (VFB213Q) /CSM/VF/VFB2-A/VFB213Q.Seq.d/
AATATATCTGTGACTTCTGTAAAAAAACAAAGAATGAAGACTCTTTCTTTATTATTTATT
GTTATTTCTTTAATCTCCTTAATNAATGCTGATGTTTTTTNAAACATAGTTGATTTTGGA
TTAGTTTCAAAAAGTAAATTTGTTAGTCAAACTTTTTCAAATATTGCACTTCCAGCATCA
TCATTTAATTTGGAAGGTGTCCAAGTTGTCATTTTAAATAAAACATCAAATCAAGCTTTT
AACCATAACTTCATTTCTACATGCACAATTGATTTCACAAGTAGTGGTAGAAAAATTGTA
TCATTCACTGCTTCAAATATTGAAAATCAATTAGTTTTCCCATCACCATATTTAGTTCAA
ATTTCTGGTAATTTAAATTTAACAGTTTCAATTTATGCATTAAATGTTGAACCACAAGAT
ATTAGTATTGCATATTATATGAAATGGGAAGAGAATAGTGGAAGTAAAACCATTGTTAAA
TTAACAAGAACTGCAACATCAGTAGTTTCAAATTTCTCAACATTTAGTGTACCACAAGGA
TCAGGTACAGGATXXXXXXXXXXGTCAAAACACAGTAATTTGTAAATCATTACCAGTTTA
TAATGATAAATTCTTAGTTAAAATGAGTAGTTGTGATAATGTAGTTAGTTTATTCAAAGG
TGCAACATACGTAACTCAAGCAGTTTATAATAATACTGTCCCATTAAAGAATGTTATTGC
TAATTTTGGTTTTTATGTTGGTTATCCAACTCCAACAACTACAACTACCACTGGTGGTGG
TTCAACATCCCATGCTACAACCTCTACCACTGGTGGTGGTTCAACATCTCACGTCACAAC
CTCTACCACTGGTGGTGGTTCAACAAGTCCATCTACCACTCACGCATCAACAACTGGTGG
TGGTCATACAACAGGTCCATCAACATCATCATCATCACATGCAACCTCTGGTCATCACGG
TTCACACTCACAATCTGAATCTGGTTCACATTCACAATCTGAATCTGGCTCACAATCTGA
ATCTGGCTCACAATCACAATCACAATCACAATCACAATCACAATCTGAATCAGGATCAGA
TTCATTCACAACCACCACAGCAGGTTCACAATCTGATTCATTCCCAGATTCATCAGAGGT
CCATCTTATACATCTGGTTCTTCTGGTTCATCAGGTACTTACATTNGAANGTAAAGGNTA
AAATTAATCTTTAAAATNAACACCCA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
NISVTSVKKQRMKTLSLLFIVISLISLXNADVFXNIVDFGLVSKSKFVSQTFSNIALPAS
SFNLEGVQVVILNKTSNQAFNHNFISTCTIDFTSSGRKIVSFTASNIENQLVFPSPYLVQ
ISGNLNLTVSIYALNVEPQDISIAYYMKWEENSGSKTIVKLTRTATSVVSNFSTFSVPQG
SGTG---

---QNTVICKSLPVYNDKFLVKMSSCDNVVSLFKGATYVTQAVYNNTVPLKNVIANFGFY
VGYPTPTTTTTTGGGSTSHATTSTTGGGSTSHVTTSTTGGGSTSPSTTHASTTGGGHTTG
PSTSSSSHATSGHHGSHSQSESGSHSQSESGSQSESGSQSQSQSQSQSQSESGSDSFTTT
TAGSQSDSFPDSSEVHLIHLVLLVHQVLTXEXKG*n*slkxtp
Translated Amino Acid sequence (All Frames) 
Frame A:
NISVTSVKKQRMKTLSLLFIVISLISLXNADVFXNIVDFGLVSKSKFVSQTFSNIALPAS
SFNLEGVQVVILNKTSNQAFNHNFISTCTIDFTSSGRKIVSFTASNIENQLVFPSPYLVQ
ISGNLNLTVSIYALNVEPQDISIAYYMKWEENSGSKTIVKLTRTATSVVSNFSTFSVPQG
SGTG---

---vktq*fvnhyqfimins*lk*vvvim*lvyskvqht*lkqfiiilsh*rmlllilvf
mlviqlqqlqlplvvvqhpmlqplplvvvqhltsqplplvvvqqvhlplthqqlvvviqq
vhqhhhhhmqplvitvhthnlnlvhihnlnlahnlnlahnhnhnhnhnhnlnqdqihsqp
pqqvhnlihsqihqrsilyiwffwfirylhxxvkxkinl*nxhp

Frame B:
iyl*ll*knke*rlflyylllfl*sp*xmlmffxt*lild*fqkvnllvklfqilhfqhh
hliwkvsklsf*ikhqiklltitsflhaqlisqvvveklyhsllqilkin*fshhhi*fk
flvi*i*qfqfmh*mlnhkilvlhii*ngkrivevkplln*qelqhq*fqisqhlvyhkd
qvqd---

---skhsnl*iitsl***ils*ne*l**cs*fiqrcnirnsssl**ycpikecyc*fwfl
cwlsnsnnynyhwwwfnipcynlyhwwwfnisrhnlyhwwwfnksiyhsrinnwwwsynr
siniiiitcnlwssrftlti*iwftfti*iwlti*iwltitititititi*irirfihnh
hsrfti*fiprfirgpsytsgssgssgtyixx*rxklifkxnt

Frame C:
yicdfckktknedsffiiycyffnllnxc*cfxkhs*fwisfkk*ic*snffkyctssii
i*fgrcpschfk*nikssf*p*lhfymhn*fhk*w*knciihcfky*ksisfpitifssn
fw*fkfnsfnlcikc*ttry*ycilyemgre*wk*nhc*inkncnissfkflni*cttri
ryr---

---QNTVICKSLPVYNDKFLVKMSSCDNVVSLFKGATYVTQAVYNNTVPLKNVIANFGFY
VGYPTPTTTTTTGGGSTSHATTSTTGGGSTSHVTTSTTGGGSTSPSTTHASTTGGGHTTG
PSTSSSSHATSGHHGSHSQSESGSHSQSESGSQSESGSQSQSQSQSQSQSESGSDSFTTT
TAGSQSDSFPDSSEVHLIHLVLLVHQVLTXEXKG*n*slkxtp
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFB213 (VFB213Q) /CSM/VF/VFB2-A/VFB213Q.Seq.d/                       1826   0.0
VFN739 (VFN739Q) /CSM/VF/VFN7-B/VFN739Q.Seq.d/                        995   0.0
VFL859 (VFL859Q) /CSM/VF/VFL8-C/VFL859Q.Seq.d/                        995   0.0
VFL453 (VFL453Q) /CSM/VF/VFL4-C/VFL453Q.Seq.d/                        995   0.0
VFK754 (VFK754Q) /CSM/VF/VFK7-C/VFK754Q.Seq.d/                        995   0.0
VFJ863 (VFJ863Q) /CSM/VF/VFJ8-C/VFJ863Q.Seq.d/                        995   0.0
VFI833 (VFI833Q) /CSM/VF/VFI8-B/VFI833Q.Seq.d/                        995   0.0
VFI764 (VFI764Q) /CSM/VF/VFI7-C/VFI764Q.Seq.d/                        995   0.0
VFI289 (VFI289Q) /CSM/VF/VFI2-D/VFI289Q.Seq.d/                        995   0.0
VFH404 (VFH404Q) /CSM/VF/VFH4-A/VFH404Q.Seq.d/                        995   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

U72746|U72746.1 Dictyostelium discoideum cysteine proteinase (cprG) mRNA, complete cds.    54  0.004  1
AL833783|AL833783.8 Mouse DNA sequence from clone RP23-287B11 on chromosome 4.    44  0.010  4
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.    36  0.012  11
AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.    34  0.018  7
AL133496|AL133496.1 Drosophila melanogaster clone BACR43C24 WORKING DRAFT SEQUENCE; 144 unordered pieces.    44  0.044  3
CF350937|CF350937.1 rl65c10.y1 Meloidogyne javanica J2 SMART pGEM Meloidogyne javanica cDNA 5', mRNA sequence.    34  0.046  3
AC116920|AC116920.2 Dictyostelium discoideum chromosome 2 map 3879572-4071762 strain AX4, complete sequence.    34  0.047  7
AC020297|AC020297.1 Drosophila melanogaster, *** SEQUENCING IN PROGRESS ***, in ordered pieces.    44  0.057  2
AL954173|AL954173.8 Zebrafish DNA sequence from clone CH211-272N13.    36  0.10  5
AC079993|AC079993.2 Homo sapiens chromosome 8 clone RP11-496M14, WORKING DRAFT SEQUENCE, 20 unordered pieces.    40  0.32  6
dna update 2003. 8.28
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AM446570_1(AM446570|pid:none) Vitis vinifera contig VV78X034573....    48   6e-04
EU557269_8(EU557269|pid:none) Teleogryllus emma mitochondrion, c...    37   1.9
CP000123_158(CP000123|pid:none) Mycoplasma capricolum subsp. cap...    35   4.1
CP001068_1063(CP001068|pid:none) Ralstonia pickettii 12J chromos...    35   4.1
CU695238_87(CU695238|pid:none) Ralstonia solanacearum strain Mol...    35   7.1
CU914168_1593(CU914168|pid:none) Ralstonia solanacearum strain I...    35   7.1
CU633749_1834(CU633749|pid:none) Cupriavidus taiwanensis str. LM...    34   9.2
protein update 2009. 2. 5
PSORT 

 psg: 0.84  gvh: 0.77  alm: 0.40  top: 0.13  tms: 0.00  mit: 0.39  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: extracellular, including cell wall
	 12.0 %: vacuolar
	 12.0 %: endoplasmic reticulum
	  8.0 %: cytoplasmic
	  8.0 %: mitochondrial
	  4.0 %: Golgi
	  4.0 %: nuclear

>> prediction for VFB213 is exc
5' end seq. ID VFB213F
5' end seq. 
>VFB213F.Seq
AATATATCTGTGACTTCTGTAAAAAAACAAAGAATGAAGACTCTTTCTTTATTATTTATT
GTTATTTCTTTAATCTCCTTAATNAATGCTGATGTTTTTTNAAACATAGTTGATTTTGGA
TTAGTTTCAAAAAGTAAATTTGTTAGTCAAACTTTTTCAAATATTGCACTTCCAGCATCA
TCATTTAATTTGGAAGGTGTCCAAGTTGTCATTTTAAATAAAACATCAAATCAAGCTTTT
AACCATAACTTCATTTCTACATGCACAATTGATTTCACAAGTAGTGGTAGAAAAATTGTA
TCATTCACTGCTTCAAATATTGAAAATCAATTAGTTTTCCCATCACCATATTTAGTTCAA
ATTTCTGGTAATTTAAATTTAACAGTTTCAATTTATGCATTAAATGTTGAACCACAAGAT
ATTAGTATTGCATATTATATGAAATGGGAAGAGAATAGTGGAAGTAAAACCATTGTTAAA
TTAACAAGAACTGCAACATCAGTAGTTTCAAATTTCTCAACATTTAGTGTACCACAAGGA
TCAGGTACAGGAT----------
Length of 5' end seq. 553
3' end seq. ID VFB213Z
3' end seq. 
>VFB213Z.Seq
----------GTCAAAACACAGTAATTTGTAAATCATTACCAGTTTATAATGATAAATTC
TTAGTTAAAATGAGTAGTTGTGATAATGTAGTTAGTTTATTCAAAGGTGCAACATACGTA
ACTCAAGCAGTTTATAATAATACTGTCCCATTAAAGAATGTTATTGCTAATTTTGGTTTT
TATGTTGGTTATCCAACTCCAACAACTACAACTACCACTGGTGGTGGTTCAACATCCCAT
GCTACAACCTCTACCACTGGTGGTGGTTCAACATCTCACGTCACAACCTCTACCACTGGT
GGTGGTTCAACAAGTCCATCTACCACTCACGCATCAACAACTGGTGGTGGTCATACAACA
GGTCCATCAACATCATCATCATCACATGCAACCTCTGGTCATCACGGTTCACACTCACAA
TCTGAATCTGGTTCACATTCACAATCTGAATCTGGCTCACAATCTGAATCTGGCTCACAA
TCACAATCACAATCACAATCACAATCACAATCTGAATCAGGATCAGATTCATTCACAACC
ACCACAGCAGGTTCACAATCTGATTCATTCCCAGATTCATCAGAGGTCCATCTTATACAT
CTGGTTCTTCTGGTTCATCAGGTACTTACATTNGAANGTAAAGGNTAAAATTAATCTTTA
AAATNAACACCCA
Length of 3' end seq. 663
Connected seq. ID VFB213P
Connected seq. 
>VFB213P.Seq
AATATATCTGTGACTTCTGTAAAAAAACAAAGAATGAAGACTCTTTCTTTATTATTTATT
GTTATTTCTTTAATCTCCTTAATNAATGCTGATGTTTTTTNAAACATAGTTGATTTTGGA
TTAGTTTCAAAAAGTAAATTTGTTAGTCAAACTTTTTCAAATATTGCACTTCCAGCATCA
TCATTTAATTTGGAAGGTGTCCAAGTTGTCATTTTAAATAAAACATCAAATCAAGCTTTT
AACCATAACTTCATTTCTACATGCACAATTGATTTCACAAGTAGTGGTAGAAAAATTGTA
TCATTCACTGCTTCAAATATTGAAAATCAATTAGTTTTCCCATCACCATATTTAGTTCAA
ATTTCTGGTAATTTAAATTTAACAGTTTCAATTTATGCATTAAATGTTGAACCACAAGAT
ATTAGTATTGCATATTATATGAAATGGGAAGAGAATAGTGGAAGTAAAACCATTGTTAAA
TTAACAAGAACTGCAACATCAGTAGTTTCAAATTTCTCAACATTTAGTGTACCACAAGGA
TCAGGTACAGGAT----------GTCAAAACACAGTAATTTGTAAATCATTACCAGTTTA
TAATGATAAATTCTTAGTTAAAATGAGTAGTTGTGATAATGTAGTTAGTTTATTCAAAGG
TGCAACATACGTAACTCAAGCAGTTTATAATAATACTGTCCCATTAAAGAATGTTATTGC
TAATTTTGGTTTTTATGTTGGTTATCCAACTCCAACAACTACAACTACCACTGGTGGTGG
TTCAACATCCCATGCTACAACCTCTACCACTGGTGGTGGTTCAACATCTCACGTCACAAC
CTCTACCACTGGTGGTGGTTCAACAAGTCCATCTACCACTCACGCATCAACAACTGGTGG
TGGTCATACAACAGGTCCATCAACATCATCATCATCACATGCAACCTCTGGTCATCACGG
TTCACACTCACAATCTGAATCTGGTTCACATTCACAATCTGAATCTGGCTCACAATCTGA
ATCTGGCTCACAATCACAATCACAATCACAATCACAATCACAATCTGAATCAGGATCAGA
TTCATTCACAACCACCACAGCAGGTTCACAATCTGATTCATTCCCAGATTCATCAGAGGT
CCATCTTATACATCTGGTTCTTCTGGTTCATCAGGTACTTACATTNGAANGTAAAGGNTA
AAATTAATCTTTAAAATNAACACCCA
Length of connected seq. 1216
Full length Seq ID -
Full length Seq. -
Length of full length seq. -