>VFB187 (VFB187Q) /CSM/VF/VFB1-D/VFB187Q.Seq.d/
ATCTTTTTTGTTAATAAAAAAGAAAGAATACCAACATGAGACTTTTATTGTGTTTAATTT
TCTTAGTTTTTGTTTTNATTTTGCATTATCAACAGTTCATTTCAAAGATACCTTTGATAA
CGATTGGGAATCAAGATGGGTTGTTAGTGATTGGCATAAAGAAGATGGTAAATCAGGTAA
ATTAGTTCATACTGCAGGTAAATGGTTTGGTGATGAAAATCAAAAAGGTATTCAAACTAG
TGAAGATGCTCGTTTCTACGCTGTATCAGCTAAATTCCCAAGTTTCAGTAACAAAGGTAA
AGATTTAGTTCTCCAATACACTGTAAAGAATGAACAAAAAGTTGATTGTGGTGGTTCATA
CATCAAGTTATTACCATCCAAATTAGATCAATCAGCATTCGATGGTGAATCTGAATATTC
CATTATGTTTGGTCCAGATGTTTGTGGTGCCTCAAAGAGAGTTCACGTCATTCTCAACTA
CAAAGGAAAGAACCATTTAATCAAGAAAGAAATCAACAAAGTTGAAACCGATCAATTAAC
TCATCAATACACTTTGXXXXXXXXXXTACCTCTTTAATGATNTCGGNGCTATCGGTTTCG
AATTATGGCNAGTTNAATCTGGNTCCATCTTTAACAATATGATCGTCCCCGACTCTGTCG
AANAAGNTAAANATTTNTNTGAAAANACCTTTGNTGCCNACCNANAANCCGAAAANAAAA
TGTTTNNCGNCCNCGAANCCGCCNAAGCTNAANANANAAANAAAGCTGACGAAAAATTAG
CTGCCGAAAAAGCTGCTGAAAAANAAGCCGAANAAGCTTATGAANAANAANAAGAAGNCG
CTGAANAAGATTTAG

SFLLIKKKEYQHETFIVFNFLSFCFXFALSTVHFKDTFDNDWESRWVVSDWHKEDGKSGK
LVHTAGKWFGDENQKGIQTSEDARFYAVSAKFPSFSNKGKDLVLQYTVKNEQKVDCGGSY
IKLLPSKLDQSAFDGESEYSIMFGPDVCGASKRVHVILNYKGKNHLIKKEINKVETDQLT
HQYTL---

---YLFNDXGAIGFELWXVXSGSIFNNMIVPDSVEXXKXXXEXTFXAXXXXEXKMFXXXE
XAXAXXXXKADEKLAAEKAAEKXAEXAYEXXXEXAEXDL

Frame A:
iffvnkkeript*dfycv*fs*flfxfciinssfqryl**rlgikmgc**la*rrw*ir*
issycr*mvw**kskrysn**rcsflrcis*ipkfq*qr*rfsspihcke*tks*lwwfi
hqvitiqirsisirw*i*ifhyvwsrclwclkessrhsqlqrkepfnqernqqs*nrsin
ssihf---

---YLFNDXGAIGFELWXVXSGSIFNNMIVPDSVEXXKXXXEXTFXAXXXXEXKMFXXXE
XAXAXXXXKADEKLAAEKAAEKXAEXAYEXXXEXAEXDL

Frame B:
SFLLIKKKEYQHETFIVFNFLSFCFXFALSTVHFKDTFDNDWESRWVVSDWHKEDGKSGK
LVHTAGKWFGDENQKGIQTSEDARFYAVSAKFPSFSNKGKDLVLQYTVKNEQKVDCGGSY
IKLLPSKLDQSAFDGESEYSIMFGPDVCGASKRVHVILNYKGKNHLIKKEINKVETDQLT
HQYTL---

---tslmxsxlsvsnygxxnlxpslti*ssptlsxkxxixxkxplxptxxpkxkcxxxxx
ppklxxxxkltkn*lpkkllkxkpxklmxxxkkxlxki*

Frame C:
lfc**krkntnmrlllcliflvfvxilhyqqfiskiplitignqdgllvigikkmvnqvn
*filqvnglvmkikkvfklvkmlvstlyqlnsqvsvtkvki*fsntl*rmnkklivvvht
ssyyhpn*inqhsmvnlniplclvqmfvvpqreftsfsttkerti*srkkstklkpin*l
intl---

---pl**xrxyrfrimasxiwxhl*qydrprlcrxx*xfx*kxlxcxpxxrkxnvxrprx
rxsxxxkxs*rkiscrksc*kxsrxsl*xxxrxr*xrf

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFB187 (VFB187Q) /CSM/VF/VFB1-D/VFB187Q.Seq.d/                       1235   0.0
VFN642 (VFN642Q) /CSM/VF/VFN6-B/VFN642Q.Seq.d/                        940   0.0
VFM435 (VFM435Q) /CSM/VF/VFM4-B/VFM435Q.Seq.d/                        940   0.0
VFM276 (VFM276Q) /CSM/VF/VFM2-D/VFM276Q.Seq.d/                        940   0.0
VFL360 (VFL360Q) /CSM/VF/VFL3-C/VFL360Q.Seq.d/                        940   0.0
VFL214 (VFL214Q) /CSM/VF/VFL2-A/VFL214Q.Seq.d/                        940   0.0
VFK631 (VFK631Q) /CSM/VF/VFK6-B/VFK631Q.Seq.d/                        940   0.0
VFK516 (VFK516Q) /CSM/VF/VFK5-A/VFK516Q.Seq.d/                        940   0.0
VFH220 (VFH220Q) /CSM/VF/VFH2-A/VFH220Q.Seq.d/                        940   0.0
VFH144 (VFH144Q) /CSM/VF/VFH1-B/VFH144Q.Seq.d/                        940   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

U36937|U36937.1 Dictyostelium discoideum calreticulin mRNA, complete cds.   940  0.0  4
BD248385|BD248385.1 Gene participating in tolerance against environmental stress.    92  4e-24  3
BZ392863|BZ392863.1 EINBW38TF EI_10_12_KB Entamoeba invadens genomic clone EINBW38, DNA sequence.    52  2e-05  2
BI876472|BI876472.1 fl72h01.y1 Sugano Kawakami zebrafish DRA Danio rerio cDNA clone 3819504 5' similar to TR:Q9PUC1 Q9PUC1 CALRETICULIN. ;, mRNA sequence.    42  3e-04  2
CB086220|CB086220.1 hj81f04.g1 Hedyotis centranthoides flower - Stage 2 (NYBG) Hedyotis centranthoides cDNA clone hj81f04, mRNA sequence.    36  4e-04  3
BI468042|BI468042.1 EST00452 Atlantic salmon Lambda Zap Express liver cDNA library Salmo salar cDNA clone LRR7-f02 5' similar to Calreticulin, mRNA sequence.    56  8e-04  1
CB937139|CB937139.1 IpCGJx13_12_E07_23 IpCGJx13 Ictalurus punctatus cDNA clone IpCGJx13_12_E07 5', mRNA sequence.    56  8e-04  1
AY342298|AY342298.1 Ictalurus punctatus ER-resident chaperone calreticulin mRNA, complete cds.    56  8e-04  1
BQ261502|BQ261502.1 fz71d12.y1 Sugano SJD adult male Danio rerio cDNA clone 5914030 5' similar to TR:Q9PUC1 Q9PUC1 CALRETICULIN. ;, mRNA sequence.    40  0.001  2
BM957414|BM957414.1 fy77g03.y1 Sugano SJD adult male Danio rerio cDNA clone 5777093 5' similar to TR:Q9PUC1 Q9PUC1 CALRETICULIN. ;, mRNA sequence.    40  0.001  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U36937_1(U36937|pid:none) Dictyostelium discoideum calreticulin ...   340   3e-92
AC133339_28(AC133339|pid:none) Oryza sativa chromosome 3 BAC OSJ...   209   1e-52
EF085019_1(EF085019|pid:none) Picea sitchensis clone WS0297_I16 ...   207   5e-52
AK318734_1(AK318734|pid:none) Arabidopsis thaliana AT1G56340 mRN...   206   6e-52
U27698_1(U27698|pid:none) Arabidopsis thaliana calreticulin (AtC...   206   6e-52
(Q40401) RecName: Full=Calreticulin; Flags: Precursor;  &T16968(...   206   6e-52
AB196794_1(AB196794|pid:none) Glycine max Gm crt-1 mRNA for calr...   206   8e-52
EU984501_1(EU984501|pid:none) Nicotiana tabacum calreticulin mRN...   204   3e-51
AY086745_1(AY086745|pid:none) Arabidopsis thaliana clone 27210 m...   203   5e-51
AY836753_1(AY836753|pid:none) Triticum aestivum calreticulin-lik...   203   5e-51

 psg: 0.70  gvh: 0.62  alm: 0.30  top: 0.27  tms: 0.07  mit: 0.20  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.31  leu: 0.31  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 24.0 %: cytoplasmic
	 24.0 %: nuclear
	 20.0 %: endoplasmic reticulum
	 12.0 %: Golgi
	 12.0 %: mitochondrial
	  4.0 %: vesicles of secretory system
	  4.0 %: peroxisomal

>> prediction for VFB187 is cyt

>VFB187F.Seq
ATCTTTTTTGTTAATAAAAAAGAAAGAATACCAACATGAGACTTTTATTGTGTTTAATTT
TCTTAGTTTTTGTTTTNATTTTGCATTATCAACAGTTCATTTCAAAGATACCTTTGATAA
CGATTGGGAATCAAGATGGGTTGTTAGTGATTGGCATAAAGAAGATGGTAAATCAGGTAA
ATTAGTTCATACTGCAGGTAAATGGTTTGGTGATGAAAATCAAAAAGGTATTCAAACTAG
TGAAGATGCTCGTTTCTACGCTGTATCAGCTAAATTCCCAAGTTTCAGTAACAAAGGTAA
AGATTTAGTTCTCCAATACACTGTAAAGAATGAACAAAAAGTTGATTGTGGTGGTTCATA
CATCAAGTTATTACCATCCAAATTAGATCAATCAGCATTCGATGGTGAATCTGAATATTC
CATTATGTTTGGTCCAGATGTTTGTGGTGCCTCAAAGAGAGTTCACGTCATTCTCAACTA
CAAAGGAAAGAACCATTTAATCAAGAAAGAAATCAACAAAGTTGAAACCGATCAATTAAC
TCATCAATACACTTTG----------

>VFB187Z.Seq
----------TACCTCTTTAATGATNTCGGNGCTATCGGTTTCGAATTATGGCNAGTTNA
ATCTGGNTCCATCTTTAACAATATGATCGTCCCCGACTCTGTCGAANAAGNTAAANATTT
NTNTGAAAANACCTTTGNTGCCNACCNANAANCCGAAAANAAAATGTTTNNCGNCCNCGA
ANCCGCCNAAGCTNAANANANAAANAAAGCTGACGAAAAATTAGCTGCCGAAAAAGCTGC
TGAAAAANAAGCCGAANAAGCTTATGAANAANAANAAGAAGNCGCTGAANAAGATTTAG

>VFB187P.Seq
ATCTTTTTTGTTAATAAAAAAGAAAGAATACCAACATGAGACTTTTATTGTGTTTAATTT
TCTTAGTTTTTGTTTTNATTTTGCATTATCAACAGTTCATTTCAAAGATACCTTTGATAA
CGATTGGGAATCAAGATGGGTTGTTAGTGATTGGCATAAAGAAGATGGTAAATCAGGTAA
ATTAGTTCATACTGCAGGTAAATGGTTTGGTGATGAAAATCAAAAAGGTATTCAAACTAG
TGAAGATGCTCGTTTCTACGCTGTATCAGCTAAATTCCCAAGTTTCAGTAACAAAGGTAA
AGATTTAGTTCTCCAATACACTGTAAAGAATGAACAAAAAGTTGATTGTGGTGGTTCATA
CATCAAGTTATTACCATCCAAATTAGATCAATCAGCATTCGATGGTGAATCTGAATATTC
CATTATGTTTGGTCCAGATGTTTGTGGTGCCTCAAAGAGAGTTCACGTCATTCTCAACTA
CAAAGGAAAGAACCATTTAATCAAGAAAGAAATCAACAAAGTTGAAACCGATCAATTAAC
TCATCAATACACTTTG----------TACCTCTTTAATGATNTCGGNGCTATCGGTTTCG
AATTATGGCNAGTTNAATCTGGNTCCATCTTTAACAATATGATCGTCCCCGACTCTGTCG
AANAAGNTAAANATTTNTNTGAAAANACCTTTGNTGCCNACCNANAANCCGAAAANAAAA
TGTTTNNCGNCCNCGAANCCGCCNAAGCTNAANANANAAANAAAGCTGACGAAAAATTAG
CTGCCGAAAAAGCTGCTGAAAAANAAGCCGAANAAGCTTATGAANAANAANAAGAAGNCG
CTGAANAAGATTTAG