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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFB178
Library VF
(Link to library)
Clone ID VFB178
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16382-1
Original site URL
Representative seq. ID VFB178P
(Link to Original site)
Representative DNA sequence 
>VFB178 (VFB178Q) /CSM/VF/VFB1-D/VFB178Q.Seq.d/
ATATATAAATCATTTAAAAATGGACGGTGAAGATGTTCAAGCTTTAGTTATCGATAACGG
TTCTGGTATGTGTAAAGCCGGTTTTGCTGGTGATGATGCCCCACGTGCTGTTTTCCCATC
AATTGTTGGTCGTCCAAGACACACTGGTGTTATGGTCGGTATGGGTCAAAAAGACTCATA
TGTAGGTGATGAAGCCCAATCAAAGAGAGGTATCTTAACCCTCAAATACCCAATTGAACA
CGGTATCGTTACCAACTGGGATGATATGGAAAAAATCTGGCATCATACTTTCTACAATGA
ACTCCGTGTTGCCCCAGAAGAACACCCAGTTCTCTTAACTGAAGCCCCATTAAATCCAAA
GXXXXXXXXXXACTGAACGTGGTTACTCATTCACCACCACTGCCGAAAGAGAAATCGTCA
GAGATATCAAAGAAAAATTAGCCTACGTCGCCCTCGACTTTGAAGCTGAAATGCAAACTG
CTGCCTCATCATCAGCCCTCGAAAAATCATACGAATTACCAGACGGTCAAGTCATCACCA
TCGGTAATGAACGTTTCCGTTGCCCAGAAGCCCTCTTCCAACCATCATTCTTAGGTATGG
AATCTGCTGGTATCCACGAAACCACCTACAACTCCATCATGAAATGTGATGTTGATATCC
GTAAAGATTTATACGGTAACGTCGTCTTATCCGGTGGTACAACTATGTTCCCAGGTATTG
CTGATCGTATGAACAAAGAATTAACTGCTTTAGCCCCATCAACCATGAAAATTAAAATCA
TTGCTCCACCAGAACGTAAATACTCTGTCTGGATTGGTGGATCTATCTTAGCTTCACTCT
CAACTTTCCAACAAATGTGGATCTCCAAAGAAGAATACGACGAATCAGGTCCATCAATGT
TCACAGAAAATGTTTCTAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
YINHLKMDGEDVQALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDSY
VGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPK
---

---TERGYSFTTTAEREIVRDIKEKLAYVALDFEAEMQTAASSSALEKSYELPDGQVITI
GNERFRCPEALFQPSFLGMESAGIHETTYNSIMKCDVDIRKDLYGNVVLSGGTTMFPGIA
DRMNKELTALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKEEYDESGPSMF
TENVS
Translated Amino Acid sequence (All Frames) 
Frame A:
iyksfkngr*rcssfsyr*rfwyv*srfcw**cptccfpincwsskthwcygrygskrli
cr**spikerylnpqipn*tryryqlg*ygknlasyflq*tpccprrtpssln*spiksk
---

---TERGYSFTTTAEREIVRDIKEKLAYVALDFEAEMQTAASSSALEKSYELPDGQVITI
GNERFRCPEALFQPSFLGMESAGIHETTYNSIMKCDVDIRKDLYGNVVLSGGTTMFPGIA
DRMNKELTALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKEEYDESGPSMF
TENVS

Frame B:
YINHLKMDGEDVQALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDSY
VGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPK
---

---lnvvthspplpkeksseiskkn*ptspstlklkckllphhqpsknhtnyqtvkssps
vmnvsvaqkpssnhhs*vwnllvstkppttps*nvmlisvkiytvtssypvvqlcsqvll
iv*tkn*ll*phqp*klksllhqnvntlsglvdls*lhsqlsnkcgspkknttnqvhqcs
qkmfl

Frame C:
i*ii*kwtvkmfkl*lsitvlvcvkpvllvmmphvlfshqllvvqdtlvlwsvwvkkthm
*vmkpnqrevs*psntqlntvslptgmiwkksgiilstmnsvlpqkntqfs*lkph*iq-
--

---*twllihhhcrkrnrqryqrkislrrprl*s*nanccliisprkiiritrrsshhhr
**tfplprsplptiilrygicwyprnhlqlhhem*c*yp*rfir*rrlirwynyvpryc*
syeqrincfspinhen*nhcstrt*ilcldwwiylsftlnfptnvdlqrrirrirsinvh
rkcf*
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFB178 (VFB178Q) /CSM/VF/VFB1-D/VFB178Q.Seq.d/                       1762   0.0
VFJ681 (VFJ681Q) /CSM/VF/VFJ6-D/VFJ681Q.Seq.d/                       1086   0.0
VFJ565 (VFJ565Q) /CSM/VF/VFJ5-C/VFJ565Q.Seq.d/                       1086   0.0
VFI228 (VFI228Q) /CSM/VF/VFI2-B/VFI228Q.Seq.d/                       1086   0.0
VFB316 (VFB316Q) /CSM/VF/VFB3-A/VFB316Q.Seq.d/                       1086   0.0
VFB206 (VFB206Q) /CSM/VF/VFB2-A/VFB206Q.Seq.d/                       1086   0.0
VFB133 (VFB133Q) /CSM/VF/VFB1-B/VFB133Q.Seq.d/                       1086   0.0
VFA683 (VFA683Q) /CSM/VF/VFA6-D/VFA683Q.Seq.d/                       1086   0.0
VFJ542 (VFJ542Q) /CSM/VF/VFJ5-B/VFJ542Q.Seq.d/                       1078   0.0
VFO747 (VFO747Q) /CSM/VF/VFO7-B/VFO747Q.Seq.d/                       1072   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

X03281|X03281.1 Dictyostelium discoideum gene for actin A8.     1045  0.0  3
AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.  1005  0.0  4
M14146|M14146.1 D.discoideum actin 15 gene, complete cds.        934  0.0  3
AC115579|AC115579.2 Dictyostelium discoideum chromosome 2 map 4915084-5005461 strain AX4, complete sequence.   934  0.0  4
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.   862  0.0  5
AC115577|AC115577.2 Dictyostelium discoideum chromosome 2 map 4657875-4914984 strain AX4, complete sequence.   793  0.0  11
X03282|X03282.1 dictyostelium discoideum gene for actin A12.     743  0.0  3
AC116956|AC116956.2 Dictyostelium discoideum chromosome 2 map 1418423-1684967 strain AX4, complete sequence.   735  0.0  6
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.   624  0.0  4
U25660|U25660.1 Dictyostelium discoideum actin gene, partial cds.   615  0.0  2
dna update 2003. 8.27
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

FN357543_13(FN357543|pid:none) Schistosoma mansoni genome sequen...   330   e-152
AL160004_3(AL160004|pid:none) Human DNA sequence from clone RP5-...   303   e-140
A03000(A03000)actin 3 - fruit fly (Drosophila melanogaster) (fra...   270   e-133
(Q54H30) RecName: Full=Major actin; AltName: Full=Actin-1; AltNa...   349   6e-95
X03282_1(X03282|pid:none) dictyostelium discoideum gene for acti...   349   6e-95
B23412(B23412)actin 12 - slime mold (Dictyostelium discoideum)        349   6e-95
ATDO(A93223;A92871;S67999;A03004)actin - slime mold (Dictyosteli...   345   1e-93
(P02576) RecName: Full=Actin, plasmodial isoform;  &ATFY(A29044;...   345   1e-93
(Q553U6) RecName: Full=Putative actin-22;  &AC116305_3(AC116305|...   345   1e-93
(P07829) RecName: Full=Actin-3; AltName: Full=Actin-3-sub 1;          342   8e-93
protein update 2009. 6.17
PSORT 

 psg: 0.75  gvh: 0.34  alm: 0.46  top: 0.53  tms: 0.00  mit: 0.15  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 40.0 %: nuclear
	  8.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: mitochondrial
	  4.0 %: vesicles of secretory system

>> prediction for VFB178 is cyt
5' end seq. ID VFB178F
5' end seq. 
>VFB178F.Seq
ATATATAAATCATTTAAAAATGGACGGTGAAGATGTTCAAGCTTTAGTTATCGATAACGG
TTCTGGTATGTGTAAAGCCGGTTTTGCTGGTGATGATGCCCCACGTGCTGTTTTCCCATC
AATTGTTGGTCGTCCAAGACACACTGGTGTTATGGTCGGTATGGGTCAAAAAGACTCATA
TGTAGGTGATGAAGCCCAATCAAAGAGAGGTATCTTAACCCTCAAATACCCAATTGAACA
CGGTATCGTTACCAACTGGGATGATATGGAAAAAATCTGGCATCATACTTTCTACAATGA
ACTCCGTGTTGCCCCAGAAGAACACCCAGTTCTCTTAACTGAAGCCCCATTAAATCCAAA
G----------
Length of 5' end seq. 361
3' end seq. ID VFB178Z
3' end seq. 
>VFB178Z.Seq
----------ACTGAACGTGGTTACTCATTCACCACCACTGCCGAAAGAGAAATCGTCAG
AGATATCAAAGAAAAATTAGCCTACGTCGCCCTCGACTTTGAAGCTGAAATGCAAACTGC
TGCCTCATCATCAGCCCTCGAAAAATCATACGAATTACCAGACGGTCAAGTCATCACCAT
CGGTAATGAACGTTTCCGTTGCCCAGAAGCCCTCTTCCAACCATCATTCTTAGGTATGGA
ATCTGCTGGTATCCACGAAACCACCTACAACTCCATCATGAAATGTGATGTTGATATCCG
TAAAGATTTATACGGTAACGTCGTCTTATCCGGTGGTACAACTATGTTCCCAGGTATTGC
TGATCGTATGAACAAAGAATTAACTGCTTTAGCCCCATCAACCATGAAAATTAAAATCAT
TGCTCCACCAGAACGTAAATACTCTGTCTGGATTGGTGGATCTATCTTAGCTTCACTCTC
AACTTTCCAACAAATGTGGATCTCCAAAGAAGAATACGACGAATCAGGTCCATCAATGTT
CACAGAAAATGTTTCTAA
Length of 3' end seq. 548
Connected seq. ID VFB178P
Connected seq. 
>VFB178P.Seq
ATATATAAATCATTTAAAAATGGACGGTGAAGATGTTCAAGCTTTAGTTATCGATAACGG
TTCTGGTATGTGTAAAGCCGGTTTTGCTGGTGATGATGCCCCACGTGCTGTTTTCCCATC
AATTGTTGGTCGTCCAAGACACACTGGTGTTATGGTCGGTATGGGTCAAAAAGACTCATA
TGTAGGTGATGAAGCCCAATCAAAGAGAGGTATCTTAACCCTCAAATACCCAATTGAACA
CGGTATCGTTACCAACTGGGATGATATGGAAAAAATCTGGCATCATACTTTCTACAATGA
ACTCCGTGTTGCCCCAGAAGAACACCCAGTTCTCTTAACTGAAGCCCCATTAAATCCAAA
G----------ACTGAACGTGGTTACTCATTCACCACCACTGCCGAAAGAGAAATCGTCA
GAGATATCAAAGAAAAATTAGCCTACGTCGCCCTCGACTTTGAAGCTGAAATGCAAACTG
CTGCCTCATCATCAGCCCTCGAAAAATCATACGAATTACCAGACGGTCAAGTCATCACCA
TCGGTAATGAACGTTTCCGTTGCCCAGAAGCCCTCTTCCAACCATCATTCTTAGGTATGG
AATCTGCTGGTATCCACGAAACCACCTACAACTCCATCATGAAATGTGATGTTGATATCC
GTAAAGATTTATACGGTAACGTCGTCTTATCCGGTGGTACAACTATGTTCCCAGGTATTG
CTGATCGTATGAACAAAGAATTAACTGCTTTAGCCCCATCAACCATGAAAATTAAAATCA
TTGCTCCACCAGAACGTAAATACTCTGTCTGGATTGGTGGATCTATCTTAGCTTCACTCT
CAACTTTCCAACAAATGTGGATCTCCAAAGAAGAATACGACGAATCAGGTCCATCAATGT
TCACAGAAAATGTTTCTAA
Length of connected seq. 909
Full length Seq ID -
Full length Seq. -
Length of full length seq. -