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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFB160
Library VF
(Link to library)
Clone ID VFB160
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID VFB160P
(Link to Original site)
Representative DNA sequence 
>VFB160 (VFB160Q) /CSM/VF/VFB1-C/VFB160Q.Seq.d/
ATNTTATTGTTAAAATAAGAGTAAATATTAAATAATTAAATATTAAAAAATGTNATCAAT
TGGTATTGATTTAGGTACAACATATTCATGTGTTGGTGTTTGGCAAAATGATCGTGTTGA
AATXXXXXXXXXXCTGGTATTCCACCAGCACCAAGAGGTGTACCACAAGTTGAAGTTACA
TTTGATGTTGATGCAAATGGTATCTTAAATGTATCAGCTGAAGATAAATCAACTGGTAAC
AAACAAAAGATTACAATTACCAATGATAAAGGTCGTCTTTCAAAAGAAGAAATTGAAAAA
ATGGTTGCTGATGCAGAGAAATTCAAACAACAAGATGAACAACAAAAAGATCGTGTTGAA
TCAAAGAATAAATTGGAAAATTATGCATTCACTGTTAAAAATTCAATTAAAGATGAAAAA
GTTGCAGCCAAAATTTCAGATTCAGATAAATCAACTATTGAATCTGAAACTGAAAGTGTA
CTCAAATGGTTAGAATCAAATCAAACCGCAGAAAAGGATGAATATGAAGATAAGATGAAA
GCCTTAGAAGCAGTTGTCAATCCAATCATGAGTAAACTCTATCAAGAAGGAGGTATGCCA
CAAGGTGGTGGTATGCCAGGTGGTATGTCAAATGATTCACCAAAGAGTTCAAACAACAAA
GTAGACGAATTAGATTGACGGAATTTTTCAGAGGAAAACATCTCTATCAAATAGATTTCT
TAAAAAATATAGTAGTAAATCAATAAGCTTCTCA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
xyc*nksky*iiky*KMXSIGIDLGTTYSCVGVWQNDRVE---

---GIPPAPRGVPQVEVTFDVDANGILNVSAEDKSTGNKQKITITNDKGRLSKEEIEKMV
ADAEKFKQQDEQQKDRVESKNKLENYAFTVKNSIKDEKVAAKISDSDKSTIESETESVLK
WLESNQTAEKDEYEDKMKALEAVVNPIMSKLYQEGGMPQGGGMPGGMSNDSPKSSNNKVD
ELD*rnfseenisik*is*ki***inkll
Translated Amino Acid sequence (All Frames) 
Frame A:
xlllk*e*ilnn*ilknvinwy*frynifmcwclak*sc*n---

---lvfhqhqevyhklklhlmlmqmvs*myqlkinqlvtnkrlqlpmikvvfqkkklkkw
llmqrnsnnkmnnkkivlnqrinwkimhsllkiqlkmkklqpkfqiqinqllnlklkvys
ng*nqikpqkrmnmkir*kp*kqlsiqs*vnsikkevchkvvvcqvvcqmihqrvqttk*
tn*idgifqrktslsnrflkkyssksisfs

Frame B:
xyc*nksky*iiky*KMXSIGIDLGTTYSCVGVWQNDRVE---

---wyststkrctts*syi*c*ckwylkcis*r*inw*qtkdynyq**rssfkrrn*kng
c*creiqttr*ttkrsc*ike*igklcihc*kfn*r*kscsqnfrfr*iny*i*n*kctq
mvriksnrrkg*i*r*deslrsscqsnhe*tlsrrryatrwwyarwyvk*ftkefkqqsr
rirlteffrgkhlyqidflknivvnq*as

Frame C:
xivkirvnik*lnikkcxqlvli*vqhihvlvfgkmivlk---

---GIPPAPRGVPQVEVTFDVDANGILNVSAEDKSTGNKQKITITNDKGRLSKEEIEKMV
ADAEKFKQQDEQQKDRVESKNKLENYAFTVKNSIKDEKVAAKISDSDKSTIESETESVLK
WLESNQTAEKDEYEDKMKALEAVVNPIMSKLYQEGGMPQGGGMPGGMSNDSPKSSNNKVD
ELD*rnfseenisik*is*ki***inkll
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFB160 (VFB160Q) /CSM/VF/VFB1-C/VFB160Q.Seq.d/                       1328   0.0
VHN188 (VHN188Q) /CSM/VH/VHN1-D/VHN188Q.Seq.d/                       1231   0.0
CHO273 (CHO273Q) /CSM/CH/CHO2-D/CHO273Q.Seq.d/                       1231   0.0
SFC829 (SFC829Q) /CSM/SF/SFC8-B/SFC829Q.Seq.d/                       1229   0.0
VHL645 (VHL645Q) /CSM/VH/VHL6-B/VHL645Q.Seq.d/                       1225   0.0
AFJ113 (AFJ113Q) /CSM/AF/AFJ1-A/AFJ113Q.Seq.d/                       1225   0.0
CFA811 (CFA811Q) /CSM/CF/CFA8-A/CFA811Q.Seq.d/                       1223   0.0
VHM303 (VHM303Q) /CSM/VH/VHM3-A/VHM303Q.Seq.d/                       1217   0.0
VHL190 (VHL190Q) /CSM/VH/VHL1-D/VHL190Q.Seq.d/                       1217   0.0
VFA790 (VFA790Q) /CSM/VF/VFA7-D/VFA790Q.Seq.d/                       1217   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

X75263|X75263.1 D.discoideum (AX3) mRNA for heat shock cognate protein (hsc70).  1181  0.0  2
L22736|L22736.1 Dictyostelium discoideum heat shock protein (hspB) gene,.  1174  0.0  1
AF025951|AF025951.1 Dictyostelium discoideum heat-shock cognate protein 70 (hsc70) mRNA, complete cds.   105  2e-39  4
M84652|M84652.1 Entamoeba heat shock protein (Hsp70) mRNA, complete cds.   125  2e-38  2
U71181|U71181.1 Cryptosporidium parvum heat shock protein (Hsp70) mRNA, complete cds.   107  1e-36  3
U69698|U69698.2 Cryptosporidium parvum heat shock protein 70 (Hsp 70) mRNA, complete cds.   107  1e-36  3
U11761|U11761.1 Cryptosporidium parvum KSU-1 heat shock 70 kDa protein (hsp70) gene, complete cds.   107  3e-36  3
AF221528|AF221528.1 Cryptosporidium parvum isolate 11 heat shock protein 70 (HSP70) gene, partial cds.   107  3e-36  3
AF221541|AF221541.1 Cryptosporidium serpentis heat shock protein 70 (HSP70) gene, partial cds.    84  4e-35  5
AX536934|AX536934.1 Sequence 535 from Patent WO02064766.          78  3e-33  4
dna update 2003. 8.27
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P36415) RecName: Full=Heat shock cognate 70 kDa protein 1;     ...   293   5e-78
S37394(S37394) dnaK-type molecular chaperone hsc70 - slime mold ...   293   5e-78
AF033852_1(AF033852|pid:none) Spinacia oleracea cytosolic heat s...   203   5e-51
FB790108_1(FB790108|pid:none) Sequence 9381 from Patent WO200803...   201   2e-50
(Q54BE0) RecName: Full=Heat shock cognate 70 kDa protein 3;           200   3e-50
AY253326_1(AY253326|pid:none) Nicotiana tabacum heat shock prote...   199   8e-50
FB790112_1(FB790112|pid:none) Sequence 9385 from Patent WO200803...   199   1e-49
(P22953) RecName: Full=Heat shock cognate 70 kDa protein 1;     ...   198   1e-49
AC147536_23(AC147536|pid:none) Medicago truncatula clone mth2-9l...   198   1e-49
AY830127_1(AY830127|pid:none) Medicago sativa heat shock protein...   198   1e-49
protein update 2009. 6.17
PSORT 

 psg: 0.87  gvh: 0.37  alm: 0.45  top: 0.27  tms: 0.00  mit: 0.17  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 36.0 %: nuclear
	  8.0 %: vacuolar
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for VFB160 is cyt
5' end seq. ID VFB160F
5' end seq. 
>VFB160F.Seq
ATNTTATTGTTAAAATAAGAGTAAATATTAAATAATTAAATATTAAAAAATGTNATCAAT
TGGTATTGATTTAGGTACAACATATTCATGTGTTGGTGTTTGGCAAAATGATCGTGTTGA
AAT----------
Length of 5' end seq. 123
3' end seq. ID VFB160Z
3' end seq. 
>VFB160Z.Seq
----------CTGGTATTCCACCAGCACCAAGAGGTGTACCACAAGTTGAAGTTACATTT
GATGTTGATGCAAATGGTATCTTAAATGTATCAGCTGAAGATAAATCAACTGGTAACAAA
CAAAAGATTACAATTACCAATGATAAAGGTCGTCTTTCAAAAGAAGAAATTGAAAAAATG
GTTGCTGATGCAGAGAAATTCAAACAACAAGATGAACAACAAAAAGATCGTGTTGAATCA
AAGAATAAATTGGAAAATTATGCATTCACTGTTAAAAATTCAATTAAAGATGAAAAAGTT
GCAGCCAAAATTTCAGATTCAGATAAATCAACTATTGAATCTGAAACTGAAAGTGTACTC
AAATGGTTAGAATCAAATCAAACCGCAGAAAAGGATGAATATGAAGATAAGATGAAAGCC
TTAGAAGCAGTTGTCAATCCAATCATGAGTAAACTCTATCAAGAAGGAGGTATGCCACAA
GGTGGTGGTATGCCAGGTGGTATGTCAAATGATTCACCAAAGAGTTCAAACAACAAAGTA
GACGAATTAGATTGACGGAATTTTTCAGAGGAAAACATCTCTATCAAATAGATTTCTTAA
AAAATATAGTAGTAAATCAATAAGCTTCTCA
Length of 3' end seq. 621
Connected seq. ID VFB160P
Connected seq. 
>VFB160P.Seq
ATNTTATTGTTAAAATAAGAGTAAATATTAAATAATTAAATATTAAAAAATGTNATCAAT
TGGTATTGATTTAGGTACAACATATTCATGTGTTGGTGTTTGGCAAAATGATCGTGTTGA
AAT----------CTGGTATTCCACCAGCACCAAGAGGTGTACCACAAGTTGAAGTTACA
TTTGATGTTGATGCAAATGGTATCTTAAATGTATCAGCTGAAGATAAATCAACTGGTAAC
AAACAAAAGATTACAATTACCAATGATAAAGGTCGTCTTTCAAAAGAAGAAATTGAAAAA
ATGGTTGCTGATGCAGAGAAATTCAAACAACAAGATGAACAACAAAAAGATCGTGTTGAA
TCAAAGAATAAATTGGAAAATTATGCATTCACTGTTAAAAATTCAATTAAAGATGAAAAA
GTTGCAGCCAAAATTTCAGATTCAGATAAATCAACTATTGAATCTGAAACTGAAAGTGTA
CTCAAATGGTTAGAATCAAATCAAACCGCAGAAAAGGATGAATATGAAGATAAGATGAAA
GCCTTAGAAGCAGTTGTCAATCCAATCATGAGTAAACTCTATCAAGAAGGAGGTATGCCA
CAAGGTGGTGGTATGCCAGGTGGTATGTCAAATGATTCACCAAAGAGTTCAAACAACAAA
GTAGACGAATTAGATTGACGGAATTTTTCAGAGGAAAACATCTCTATCAAATAGATTTCT
TAAAAAATATAGTAGTAAATCAATAAGCTTCTCA
Length of connected seq. 744
Full length Seq ID -
Full length Seq. -
Length of full length seq. -