>VFB150 (VFB150Q) /CSM/VF/VFB1-C/VFB150Q.Seq.d/
XXXXXXXXXXCANCNATTCCATGTAAGAAAACTCAAACCTTCTCTACATACTNTGATAAT
CAACCAGGTGTATTAATTCAAGTTTATGAAGGTGAACGTGCTATGACCAAAGATAATAAT
TTATTGGGTNAATTTGAACTTTCTGGTATTCCACCAGCA

---XIPCKKTQTFSTYXDNQPGVLIQVYEGERAMTKDNNLLGXFELSGIPPA

Frame A:
---xxsm*ensnllyil**strcinssl*r*tcydqr**figxi*tfwysts


Frame B:
---XIPCKKTQTFSTYXDNQPGVLIQVYEGERAMTKDNNLLGXFELSGIPPA


Frame C:
---xfhvrklkpslhtxiinqvy*fkfmkvnvl*pkiiiywvnlnflvfhq

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHP236 (VHP236Q) /CSM/VH/VHP2-B/VHP236Q.Seq.d/                        274   4e-73
VHP224 (VHP224Q) /CSM/VH/VHP2-A/VHP224Q.Seq.d/                        274   4e-73
VHN562 (VHN562Q) /CSM/VH/VHN5-C/VHN562Q.Seq.d/                        274   4e-73
VHK295 (VHK295Q) /CSM/VH/VHK2-D/VHK295Q.Seq.d/                        274   4e-73
VHJ764 (VHJ764Q) /CSM/VH/VHJ7-C/VHJ764Q.Seq.d/                        274   4e-73
VHH751 (VHH751Q) /CSM/VH/VHH7-C/VHH751Q.Seq.d/                        274   4e-73
VHH614 (VHH614Q) /CSM/VH/VHH6-A/VHH614Q.Seq.d/                        274   4e-73
VHH124 (VHH124Q) /CSM/VH/VHH1-A/VHH124Q.Seq.d/                        274   4e-73
VHE119 (VHE119Q) /CSM/VH/VHE1-A/VHE119Q.Seq.d/                        274   4e-73
VHD566 (VHD566Q) /CSM/VH/VHD5-C/VHD566Q.Seq.d/                        274   4e-73

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

X75263|X75263.1 D.discoideum (AX3) mRNA for heat shock cognate protein (hsc70).   274  3e-70  1
L22736|L22736.1 Dictyostelium discoideum heat shock protein (hspB) gene,.   274  3e-70  1
BI946540|BI946540.1 bEST145.3' Honeybee brain cDNA library Apis mellifera cDNA 3' similar to heat shock cognate 70kDa protein, mRNA sequence.   117  4e-23  1
BI504420|BI504420.1 BB170011B10C03 Bee Brain Normalized/Subtracted Library, BB17 Apis mellifera cDNA clone BB170011B10C03 5', mRNA sequence.   117  4e-23  1
BX562466|BX562466.1 Glossina morsitans morsitans (Tseatse fly) EST from adult infected  midgut library, clone Tse60h02_q1c.   109  1e-20  1
BX569171|BX569171.1 Glossina morsitans morsitans (Tseatse fly) EST from adult infected  midgut library, clone Tse97h07_q1c.   109  1e-20  1
BX566698|BX566698.1 Glossina morsitans morsitans (Tseatse fly) EST from adult infected  midgut library, clone Tse83g02_q1c.   109  1e-20  1
BX559878|BX559878.1 Glossina morsitans morsitans (Tseatse fly) EST from adult infected  midgut library, clone Tse46b09_q1c.   109  1e-20  1
BI773193|BI773193.1 kq59e05.y1 TBN95TM-SSR Strongyloides stercoralis cDNA 5' similar to SW:HS70_ONCVO P11503 HEAT SHOCK 70 KD PROTEIN ;, mRNA sequence.    90  6e-20  2
BI773038|BI773038.1 kq28d03.y1 TBN95TM-SSR Strongyloides stercoralis cDNA 5' similar to SW:HS70_ONCVO P11503 HEAT SHOCK 70 KD PROTEIN ;, mRNA sequence.    90  6e-20  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P36415) RecName: Full=Heat shock cognate 70 kDa protein 1;     ...    98   1e-19
S37394(S37394) dnaK-type molecular chaperone hsc70 - slime mold ...    98   1e-19
U20256_1(U20256|pid:none) Ceratitis capitata heat shock-like pro...    91   1e-17
AY084193_1(AY084193|pid:none) Drosophila melanogaster RH04426 fu...    91   1e-17
AF252687_1(AF252687|pid:none) Drosophila simulans strain sim1 he...    91   1e-17
AY748350_1(AY748350|pid:none) Fenneropenaeus chinensis heat shoc...    91   1e-17
EF091692_1(EF091692|pid:none) Marsupenaeus japonicus heat shock ...    91   1e-17
DQ663761_1(DQ663761|pid:none) Marsupenaeus japonicus heat shock ...    91   1e-17
AY150371_1(AY150371|pid:none) Cotesia rubecula heat-shock protei...    91   1e-17
EF495128_1(EF495128|pid:none) Litopenaeus vannamei heat shock co...    91   1e-17

 psg: 0.79  gvh: 0.26  alm: 0.59  top: 0.53  tms: 0.00  mit: 0.22  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: nuclear
	 32.0 %: cytoplasmic
	  8.0 %: cytoskeletal
	  8.0 %: mitochondrial

>> prediction for VFB150 is nuc

>VFB150Z.Seq
----------CANCNATTCCATGTAAGAAAACTCAAACCTTCTCTACATACTNTGATAAT
CAACCAGGTGTATTAATTCAAGTTTATGAAGGTGAACGTGCTATGACCAAAGATAATAAT
TTATTGGGTNAATTTGAACTTTCTGGTATTCCACCAGCA