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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFM148
Library AF
(Link to library)
Clone ID AFM148
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID AFM148P
(Link to Original site)
Representative DNA sequence 
>AFM148 (AFM148Q) /CSM/AF/AFM1-B/AFM148Q.Seq.d/
AAATTAATTTTGTGTGTGGTGTGCCTTTGATTTGTATTTGTAATAAAAAAAATAATAATA
ATAATAATAATAATAATTTAAAAAATAAAAATAAAAATAAAAATCTAAAAATTAAATAAT
TAGAAATAATAAAAAAAAAATAATAATAAAAAAATATATATAAAAAAACAGTATGATTAG
TGCATTATTCTTAATGAATGGAAAAGGAGAGGTGTTAATATCACGTATCTATAGAGATGA
TATTAGCCGNGGTGTTGCAAATGCATTCAGATTAGAAGTTATTGGATCTCAAGAAACAAG
ATCACCAGTAAAATTAATTGGATCAACTTCATTTATGTATATTAAAGTTGGTAATATTTA
TATTGTTGGTGTATCAAGACAAAATGTTAATGCATGTATGGTATTCGAAGTATTACACCA
ATTGGTTGACATTTTTAAATCATATTTCGATAATTTAGATGAAGATTCAATTAGAAATAA
TTTTGTTTTAGTTTATGAGTTATTAGATGAAATTTTAGATTTTGGTTACCCACAAAATTG
TTCAACAGATGTATTAAAACTTTATATTACTCAAGGXXXXXXXXXXTGTCAGCAGAAGGT
AATATATTAAGAGCAGATGTATCAGGACAGGTTATGATGAAATGTTTCCTTTCTGGTATG
CCAGAATGTAAATTTGGTATGAATGATAAGGTTATTATGGATAGAGAGAAATCAACAAAT
GGTGGTAGTGCCGCTAGAAGTGGAGCACGTCGTGCAAATGGTATTGAAATCGATGATATT
ACATTCCATCAATGTGTACGTCTTGGTAAGTTTGATAGTGATCGTACAGTAAGTTTCATT
CCACCAGATGGTGAATTCGAATTGATGCGTTATCGTACCACTGAACATATCAATCTACCA
TTCAAAGTTATACCAATCGTTAGAGAGATGGGTAGAACTCGTTTAGAGTGCTCCGTCACT
GTTAAATCAAATTTTAGTAGTAAAATGTTTGGTGCAAATGTTAAAGTAATCATTCCAACA
CCTAAAAATACCGCTGTCTGTAAGATTGTCGTTGCAGCTGGTAAGGCTAAATATATGCCA
GAACAAGATGCTATCATTTGGAGAATTAGAAGATTCCCTGGTGATACTGAATTCACTCTC
AGAGCTGAAGTTGAACTTATGGCATCCGTTAATCTCGATAAAAAAGCTTGGTCACGTCCA
CCAATCTCTATGGAATTCCAAGTTACTATGTTCACTGCCTCTGGTTTCTCTGTTCGTTTC
CTTAAAGTCGTNAAAAAAGTAATTATACACCAATTAAATGGGTTAGATATTTAACAAAAG
CTGGTACCTATCAAAATCGTA
sequence update 2009. 4. 3
Translated Amino Acid sequence 
n*fcvwcafdlyl**kk********fkk*k*k*kskn*iirnnkkkiiikkyi*KNSMIS
ALFLMNGKGEVLISRIYRDDISRGVANAFRLEVIGSQETRSPVKLIGSTSFMYIKVGNIY
IVGVSRQNVNACMVFEVLHQLVDIFKSYFDNLDEDSIRNNFVLVYELLDEILDFGYPQNC
STDVLKLYITQ---

---SAEGNILRADVSGQVMMKCFLSGMPECKFGMNDKVIMDREKSTNGGSAARSGARRAN
GIEIDDITFHQCVRLGKFDSDRTVSFIPPDGEFELMRYRTTEHINLPFKVIPIVREMGRT
RLECSVTVKSNFSSKMFGANVKVIIPTPKNTAVCKIVVAAGKAKYMPEQDAIIWRIRRFP
GDTEFTLRAEVELMASVNLDKKAWSRPPISMEFQVTMFTASGFSVRFLKVVKKVIIHQLN
GLDI*qklvpikiv
Translated Amino Acid sequence (All Frames) 
Frame A:
klilcvvcl*fvfvikkiiiiiiiii*kikikiki*klnn*k**kknnnkkiyikkqyd*
ciilnewkrrgvnityl*r*y*pxcckciqirsywisrnkitskinwinfiyvy*sw*yl
ycwciktkc*cmygirsitpig*hf*iifr*fr*rfn*k*fcfsl*vir*nfrfwlptkl
fnrciktlyysr---

---cqqkviy*eqmyqdrl**nvsflvcqnvnlv*mirllwiernqqmvvvplevehvvq
mvlksmilhsinvyvlvslivivq*vsfhqmvnsn*cvivplnisiyhsklyqslerwve
lv*sapsllnqilvvkclvqmlk*sfqhlkiplsvrlslqlvrlnicqnkmlsfgeleds
lvilnslselklnlwhplisikklghvhqslwnskllcslplvslfvslksxkk*lytn*
mg*ifnkswylsks

Frame B:
n*fcvwcafdlyl**kk********fkk*k*k*kskn*iirnnkkkiiikkyi*KNSMIS
ALFLMNGKGEVLISRIYRDDISRGVANAFRLEVIGSQETRSPVKLIGSTSFMYIKVGNIY
IVGVSRQNVNACMVFEVLHQLVDIFKSYFDNLDEDSIRNNFVLVYELLDEILDFGYPQNC
STDVLKLYITQ---

---vsrr*yiksrcirtgydemfpfwyarm*iwye**gyyg*reinkww*cr*kwstsck
wy*nr*yyipsmctsw*v***syskfhstrw*iridalsyh*tyqstiqsytnr*rdg*n
sfrvlrhc*ikf***nvwckc*snhsnt*kyrcl*dcrcsw*g*iyartrcyhlen*kip
w*y*ihsqs*s*tygir*sr*kslvtstnlygipsyyvhclwflcsfp*srxksnytpik
wvryltkagtyqnr

Frame C:
infvcgvplicicnkknnnnnnnnnlknknknknlkik*leiikkk***kniykktv*lv
hys**mekerc*yhvsiemilaxvlqmhsd*klldlkkqdhq*n*ldqlhlcilklvifi
llvyqdkmlmhvwyskyytnwltflnhisii*mkiqleiilf*fmsy*mkf*ilvthkiv
qqmy*nfillk---

---SAEGNILRADVSGQVMMKCFLSGMPECKFGMNDKVIMDREKSTNGGSAARSGARRAN
GIEIDDITFHQCVRLGKFDSDRTVSFIPPDGEFELMRYRTTEHINLPFKVIPIVREMGRT
RLECSVTVKSNFSSKMFGANVKVIIPTPKNTAVCKIVVAAGKAKYMPEQDAIIWRIRRFP
GDTEFTLRAEVELMASVNLDKKAWSRPPISMEFQVTMFTASGFSVRFLKVVKKVIIHQLN
GLDI*qklvpikiv
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFM148 (AFM148Q) /CSM/AF/AFM1-B/AFM148Q.Seq.d/                       2218   0.0
AFM745 (AFM745Q) /CSM/AF/AFM7-B/AFM745Q.Seq.d/                       1439   0.0
CFJ660 (CFJ660Q) /CSM/CF/CFJ6-C/CFJ660Q.Seq.d/                       1269   0.0
SFJ890 (SFJ890Q) /CSM/SF/SFJ8-D/SFJ890Q.Seq.d/                       1259   0.0
AFJ822 (AFJ822Q) /CSM/AF/AFJ8-A/AFJ822Q.Seq.d/                       1245   0.0
SFL260 (SFL260Q) /CSM/SF/SFL2-C/SFL260Q.Seq.d/                       1223   0.0
AFN610 (AFN610Q) /CSM/AF/AFN6-A/AFN610Q.Seq.d/                       1132   0.0
AFN325 (AFN325Q) /CSM/AF/AFN3-B/AFN325Q.Seq.d/                        763   0.0
FC-AB28 (FC-AB28Q) /CSM/FC/FC-AB/FC-AB28Q.Seq.d/                      759   0.0
AFK120 (AFK120Q) /CSM/AF/AFK1-A/AFK120Q.Seq.d/                        646   0.0
own update 2009. 4. 3
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ346893) Dictyostelium discoideum cDNA clone:dda25o11, 3' ...  1491   0.0    1
(BJ347191) Dictyostelium discoideum cDNA clone:dda26j11, 3' ...  1451   0.0    2
(AC116977) Dictyostelium discoideum chromosome 2 map 5515173...  1358   0.0    2
(U44890) Dictyostelium discoideum adaptin complex medium cha...  1342   0.0    2
(BJ375934) Dictyostelium discoideum cDNA clone:ddc20g16, 3' ...  1265   0.0    1
(BJ402191) Dictyostelium discoideum cDNA clone:dds20d24, 3' ...  1251   0.0    3
(BJ401528) Dictyostelium discoideum cDNA clone:dds23g16, 3' ...  1233   0.0    2
(BJ344686) Dictyostelium discoideum cDNA clone:dda20l06, 3' ...  1168   0.0    2
(BJ345539) Dictyostelium discoideum cDNA clone:dda28c04, 3' ...  1132   0.0    1
(BJ329552) Dictyostelium discoideum cDNA clone:dda25o11, 5' ...   799   0.0    2
dna update 2009. 4. 9
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC116977_67(AC116977|pid:none) Dictyostelium discoideum chromoso...   424   e-126
(P54672) RecName: Full=AP-2 complex subunit mu; AltName: Full=Mu...   424   e-126
AK159534_1(AK159534|pid:none) Mus musculus osteoclast-like cell ...   243   e-106
AF467697_1(AF467697|pid:none) Dermacentor variabilis clathrin-ad...   226   e-101
AK304510_1(AK304510|pid:none) Homo sapiens cDNA FLJ53962 complet...   241   e-101
AB261064_1(AB261064|pid:none) Entamoeba histolytica gene for mu ...   205   4e-94
EF146351_1(EF146351|pid:none) Populus trichocarpa clone WS0117_H...   264   6e-73
EF678386_1(EF678386|pid:none) Picea sitchensis clone WS02919_E02...   263   3e-72
FJ664261_1(FJ664261|pid:none) Annona cherimola AP-2 complex subu...   261   9e-72
BX294009_23(BX294009|pid:none) Neurospora crassa DNA linkage gro...   253   9e-71
protein update 2009. 4. 6
PSORT 

 psg: 0.00  gvh: 0.00  alm: 0.00  top: 0.00  tms: 0.00  mit: 0.00  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	100.0 %: 

>> prediction for AFM148 is

5' end seq. ID AFM148F
5' end seq. 
>AFM148F.Seq
AAATTAATTTTGTGTGTGGTGTGCCTTTGATTTGTATTTGTAATAAAAAAAATAATAATA
ATAATAATAATAATAATTTAAAAAATAAAAATAAAAATAAAAATCTAAAAATTAAATAAT
TAGAAATAATAAAAAAAAAATAATAATAAAAAAATATATATAAAAAAACAGTATGATTAG
TGCATTATTCTTAATGAATGGAAAAGGAGAGGTGTTAATATCACGTATCTATAGAGATGA
TATTAGCCGNGGTGTTGCAAATGCATTCAGATTAGAAGTTATTGGATCTCAAGAAACAAG
ATCACCAGTAAAATTAATTGGATCAACTTCATTTATGTATATTAAAGTTGGTAATATTTA
TATTGTTGGTGTATCAAGACAAAATGTTAATGCATGTATGGTATTCGAAGTATTACACCA
ATTGGTTGACATTTTTAAATCATATTTCGATAATTTAGATGAAGATTCAATTAGAAATAA
TTTTGTTTTAGTTTATGAGTTATTAGATGAAATTTTAGATTTTGGTTACCCACAAAATTG
TTCAACAGATGTATTAAAACTTTATATTACTCAAGGXXXXXXXXXX
Length of 5' end seq. 586
3' end seq. ID AFM148Z
3' end seq. 
>AFM148Z.Seq
XXXXXXXXXXTGTCAGCAGAAGGTAATATATTAAGAGCAGATGTATCAGGACAGGTTATG
ATGAAATGTTTCCTTTCTGGTATGCCAGAATGTAAATTTGGTATGAATGATAAGGTTATT
ATGGATAGAGAGAAATCAACAAATGGTGGTAGTGCCGCTAGAAGTGGAGCACGTCGTGCA
AATGGTATTGAAATCGATGATATTACATTCCATCAATGTGTACGTCTTGGTAAGTTTGAT
AGTGATCGTACAGTAAGTTTCATTCCACCAGATGGTGAATTCGAATTGATGCGTTATCGT
ACCACTGAACATATCAATCTACCATTCAAAGTTATACCAATCGTTAGAGAGATGGGTAGA
ACTCGTTTAGAGTGCTCCGTCACTGTTAAATCAAATTTTAGTAGTAAAATGTTTGGTGCA
AATGTTAAAGTAATCATTCCAACACCTAAAAATACCGCTGTCTGTAAGATTGTCGTTGCA
GCTGGTAAGGCTAAATATATGCCAGAACAAGATGCTATCATTTGGAGAATTAGAAGATTC
CCTGGTGATACTGAATTCACTCTCAGAGCTGAAGTTGAACTTATGGCATCCGTTAATCTC
GATAAAAAAGCTTGGTCACGTCCACCAATCTCTATGGAATTCCAAGTTACTATGTTCACT
GCCTCTGGTTTCTCTGTTCGTTTCCTTAAAGTCGTNAAAAAAGTAATTATACACCAATTA
AATGGGTTAGATATTTAACAAAAGCTGGTACCTATCAAAATCGTA
Length of 3' end seq. 765
Connected seq. ID AFM148P
Connected seq. 
>AFM148P.Seq
AAATTAATTTTGTGTGTGGTGTGCCTTTGATTTGTATTTGTAATAAAAAAAATAATAATA
ATAATAATAATAATAATTTAAAAAATAAAAATAAAAATAAAAATCTAAAAATTAAATAAT
TAGAAATAATAAAAAAAAAATAATAATAAAAAAATATATATAAAAAAACAGTATGATTAG
TGCATTATTCTTAATGAATGGAAAAGGAGAGGTGTTAATATCACGTATCTATAGAGATGA
TATTAGCCGNGGTGTTGCAAATGCATTCAGATTAGAAGTTATTGGATCTCAAGAAACAAG
ATCACCAGTAAAATTAATTGGATCAACTTCATTTATGTATATTAAAGTTGGTAATATTTA
TATTGTTGGTGTATCAAGACAAAATGTTAATGCATGTATGGTATTCGAAGTATTACACCA
ATTGGTTGACATTTTTAAATCATATTTCGATAATTTAGATGAAGATTCAATTAGAAATAA
TTTTGTTTTAGTTTATGAGTTATTAGATGAAATTTTAGATTTTGGTTACCCACAAAATTG
TTCAACAGATGTATTAAAACTTTATATTACTCAAGG----------TGTCAGCAGAAGGT
AATATATTAAGAGCAGATGTATCAGGACAGGTTATGATGAAATGTTTCCTTTCTGGTATG
CCAGAATGTAAATTTGGTATGAATGATAAGGTTATTATGGATAGAGAGAAATCAACAAAT
GGTGGTAGTGCCGCTAGAAGTGGAGCACGTCGTGCAAATGGTATTGAAATCGATGATATT
ACATTCCATCAATGTGTACGTCTTGGTAAGTTTGATAGTGATCGTACAGTAAGTTTCATT
CCACCAGATGGTGAATTCGAATTGATGCGTTATCGTACCACTGAACATATCAATCTACCA
TTCAAAGTTATACCAATCGTTAGAGAGATGGGTAGAACTCGTTTAGAGTGCTCCGTCACT
GTTAAATCAAATTTTAGTAGTAAAATGTTTGGTGCAAATGTTAAAGTAATCATTCCAACA
CCTAAAAATACCGCTGTCTGTAAGATTGTCGTTGCAGCTGGTAAGGCTAAATATATGCCA
GAACAAGATGCTATCATTTGGAGAATTAGAAGATTCCCTGGTGATACTGAATTCACTCTC
AGAGCTGAAGTTGAACTTATGGCATCCGTTAATCTCGATAAAAAAGCTTGGTCACGTCCA
CCAATCTCTATGGAATTCCAAGTTACTATGTTCACTGCCTCTGGTTTCTCTGTTCGTTTC
CTTAAAGTCGTNAAAAAAGTAATTATACACCAATTAAATGGGTTAGATATTTAACAAAAG
CTGGTACCTATCAAAATCGTA
Length of connected seq. 1331
Full length Seq ID -
Full length Seq. -
Length of full length seq. -